6种杀菌剂对黄瓜尖孢镰刀菌的毒力测定试验
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摘要
旨在采用含毒培养基生长速率法测定6种杀菌剂的抑制效果。以菌落直径为实验观察指标,测量对照组和处理组的菌落直径,以抑制率测定杀菌剂对尖孢镰刀菌的抑制效果。试验结果表明:药后72 h,杀菌剂丙环唑的EC_(50)值为0.9039 mg/L,其毒力指数最大,对尖孢镰刀菌的抑制效果最佳;代森、异菌肽的EC_(50)值为2.8939、6.8186 mg/L,效果较好;醚菌酯、嘧霉胺的EC_(50)值为29.3951、30.4126 mg/L,效果一般;苯醚甲环唑的EC_(50)值为143.2943 mg/L,毒力指数最小,抑制效果较差。综上,丙环唑对尖孢镰刀菌的抑制效果最佳。
The aim of this studyis to identify the inhibitory effects of six fungicides by the growth rate method on toxic culture medium. The colony diameterwasused as observation index. The colony diameters of the control group and the treatment group were detected, and the inhibitory effects offungicides on Fusarium Oxysporum were measured by inhibition ratio. The results showed that72 hours after medication, propiconazole had the maximum toxicity index and the best inhibition effect onFusarium oxysporum with EC_(50) value of0.9039 mg/L, followed by mancozeb and isopeptide, with the EC_(50) value of 2.8939 mg/L and 6.8186 mg/L,respectively. Kresoxim-methyl and pyrimethanil had normal effects,with EC_(50) value of 29.3951 mg/L and30.4126 mg/L, respectively. Difenoconazole had the minimumtoxicity index and the least effect, with EC_(50) value of 143.2943 mg/L. In conclusion, propiconazole hasthe best inhibition effect.
The aim of this studyis to identify the inhibitory effects of six fungicides by the growth rate method on toxic culture medium. The colony diameterwasused as observation index. The colony diameters of the control group and the treatment group were detected, and the inhibitory effects offungicides on Fusarium Oxysporum were measured by inhibition ratio. The results showed that72 hours after medication, propiconazole had the maximum toxicity index and the best inhibition effect onFusarium oxysporum with EC_(50) value of0.9039 mg/L, followed by mancozeb and isopeptide, with the EC_(50) value of 2.8939 mg/L and 6.8186 mg/L,respectively. Kresoxim-methyl and pyrimethanil had normal effects,with EC_(50) value of 29.3951 mg/L and30.4126 mg/L, respectively. Difenoconazole had the minimumtoxicity index and the least effect, with EC_(50) value of 143.2943 mg/L. In conclusion, propiconazole hasthe best inhibition effect.
引文
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[16]苗则彦,赵奎华,刘长远,等.内生细菌B504的鉴定及对黄瓜枯萎病的生防作用[J].植物保护,2009,35(6):73-77.
[17]郑爱萍,闰敏,李平,等.黄瓜枯萎病新型抑制蛋白L37的研究[J].园艺学报,2005,32(6):1102-1104.
[18]刘金秀,马正,申屠旭萍,等.黄瓜枯萎病拮抗放线菌筛选及其生防作用鉴定[J].园艺学报,2012,39(6):1123-1130.
[19]潘争艳,刘伟成,裘季燕,等.放线菌111-61和A-21对蔬菜枯萎病和灰霉病的控制作用[J].华北农学报,2005,20(4):92-97.
[20]潘荣光,刘明霞.黄瓜枯萎病菌的拮抗放线菌筛选[J].沈阳农业大学学报,2008,39(4):492-494.
[21]李晓虹,裴永娜,李学锋,等.几株农用拮抗链霉菌的初步研究[J].微生物学杂志,2006,26(l):26-28.
[22]谷祖敏,庄敬华,高增贵,等.黄瓜枯萎病菌无毒突变株的稳定性[J].植物保护学报,2003,30(3):331-332.
[2]毛爱军,张峰,张丽蓉,等.不同黄瓜材料对枯萎病的抗性评价[J].华北农学报,2008,23(2):214-216.
[3]刘爱荣,陈双臣,陈凯,等.哈茨木霉对黄瓜尖抱镰刀菌的抑制作用和抗性相关基因表达[J].植物保护学报,2010,30(:3)25o-254.
[4]刘爱荣,陈双臣,晋文娟,等.哈茨木霉对接种尖抱镰刀菌后黄瓜根系次生代谢物的影响[J].中国生物防治学报,2012,28(4):545-551.
[5]庄敬华,高增贵,杨长城,等.绿色木霉菌T23对黄瓜枯萎病防治效果及其几种防御酶活性的影响[J].植物病理学报,2005,35(2:)179-183.
[6]朱廷恒,邢小平,孙顺娣.木霉T97菌株对几种植物病原真菌的拮抗作用机制和温室防治试验[J].植物保护学报,2004,31(2):139-144.
[7]王倡宪,李晓林,宋福强,等.两种丛枝菌根真菌对黄瓜苗期枯萎病的防效及根系抗病相关酶活性的影响[J].中国生态农业学报,2012,20(l):53-57.
[8]Bradley G G,Punj Z K.Postseontaining Futoreseent Pseudomonadssu Press Fusarium root and stemrot development on greenhouse cucumber[J].Candaian Journal of Mierobiology,2010,56(11):896-905.
[9]张铎,陶秀娥,梁艳,等.黄瓜枯萎病拮抗内生菌BPT-18的鉴定及抗菌物质性质[J].农药,2012,51(6):464-467.
[10]高芬,马利平,乔雄梧,等.蜡质芽抱杆菌Bc98-I发酵液与抑菌粗提物对黄瓜枯萎病菌的抑菌特性研究[J].中国生态农业学报,2006,14(l):190-192.
[11]张璐,丁延芹,杜秉海,等.黄瓜枯萎病病原拮抗细菌Ds-1菌株鉴定及其生防效果研究[J].园艺学报,2010,37(4):575-580.
[12]Chung S,Kim D.Eseheriehia eoli can Produce recombinant ehitinase in the soil to control the Pahtogene by Fusarimu oxysporiunwithout colonization[J].Jourrnal of Mierobiology nad Biotechnolog,2007,17(3):474-480.
[13]赵新海,钟丽娟,徐冲,等.康地蕾得对黄瓜枯萎病的室内毒力测定及田间防效试验[J].农药,2008,47(9):696-698.
[14]许煌泉,唐玮宁,郑有丽,等.筛选假单胞菌株M18防治大棚黄瓜枯萎病害[J].上海交通大学学报,1999,33(2):210-213
[15]王灿华,祝新德,许煌泉,等.假单胞菌株M18分泌梭基吩嗦抑制黄瓜枯萎病害[J].上海交通大学学报,2000,34(11):1574-1578.
[16]苗则彦,赵奎华,刘长远,等.内生细菌B504的鉴定及对黄瓜枯萎病的生防作用[J].植物保护,2009,35(6):73-77.
[17]郑爱萍,闰敏,李平,等.黄瓜枯萎病新型抑制蛋白L37的研究[J].园艺学报,2005,32(6):1102-1104.
[18]刘金秀,马正,申屠旭萍,等.黄瓜枯萎病拮抗放线菌筛选及其生防作用鉴定[J].园艺学报,2012,39(6):1123-1130.
[19]潘争艳,刘伟成,裘季燕,等.放线菌111-61和A-21对蔬菜枯萎病和灰霉病的控制作用[J].华北农学报,2005,20(4):92-97.
[20]潘荣光,刘明霞.黄瓜枯萎病菌的拮抗放线菌筛选[J].沈阳农业大学学报,2008,39(4):492-494.
[21]李晓虹,裴永娜,李学锋,等.几株农用拮抗链霉菌的初步研究[J].微生物学杂志,2006,26(l):26-28.
[22]谷祖敏,庄敬华,高增贵,等.黄瓜枯萎病菌无毒突变株的稳定性[J].植物保护学报,2003,30(3):331-332.