F1-ATPase转动马达的随机动力学研究
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摘要
通过建立物理模型研究了马达F1-ATPase转动的过程,得到理想条件下马达F1-ATPase转动的角速度随ATP浓度、摩擦系数的变化规律.同时,在热涨落环境下,计算了马达F1-ATPase向前和向后转动的概率之比,修正了理想条件下马达的转动模型,得到与实验吻合的结果.
A physical model to investigate F1-ATPase rotation was developed in the present work.Analytical expression for mean rotational velocity was obtained as a function of external control variable nucleotide concentration [ATP]and friction coefficientγL.Furthermore,the ratio of forward to backward rotational motions in the presence of thermal fluctuation was used to modify analytical expression of mean rotational velocity.The obtained results agreed well with experimental data.
A physical model to investigate F1-ATPase rotation was developed in the present work.Analytical expression for mean rotational velocity was obtained as a function of external control variable nucleotide concentration [ATP]and friction coefficientγL.Furthermore,the ratio of forward to backward rotational motions in the presence of thermal fluctuation was used to modify analytical expression of mean rotational velocity.The obtained results agreed well with experimental data.
引文
[1]Ruben P C,Sancho J M.Theoretical analysis of the FATPase experimental data[J].Biophysical Journal,2010,98:2591
[2]Yasuda R,Noji H,Itoh H.Resolution of distinct rotational substeps by submillisecond kinetic analysis of F1-ATPase[J].Nature,2001,410(19):898
[3]Muneyuki E,Shoichi T,Tetsuaki O.Thermodynamic efficiency and mechanochemical coupling of F1-ATPase[J].Proc Natl Acad Sci,2011,108(44):17951
[4]Muneyuki E,Shoichi T,Tetsuaki O.Nonequilibrium energetics of a single F1-ATPase molecule[J].PRL,2010,104(19):198103
[5]Yasuda R,Noji H,Yoshida M.F1-ATPase is a highly efficient molecular motor that rotates with discrete 120steps[J].Cell,1998,93(7):1117
[6]Watanabe-Nakayama T,Toyabe S,Muneyuki E.Effect of external torque on the ATP-driven rotation of F1-ATPase[J].Biophys,2008,366(4):951
[7]Hirono-Hara Y,Ishizuka K,Noji H.Activation of pausing F1motor by external force[J].Proc Natl Acad Sci,2005,102(12):4288
[8]Spetzler D,York J,Frasch W.Microsecond timescale rotation measurements of single F1-ATPase molecules[J].Biochemistry,2006,45(10):3117
[9]包景东.经典和量子耗散系统的随机模拟方法[M].北京:科学出版社,2009
[10]Muneyuki E,Watanabe-Nakayama T,Noji H.Single molecule energetics of F1-ATPase motor[J].Biophys,2007,92(5):1806
[2]Yasuda R,Noji H,Itoh H.Resolution of distinct rotational substeps by submillisecond kinetic analysis of F1-ATPase[J].Nature,2001,410(19):898
[3]Muneyuki E,Shoichi T,Tetsuaki O.Thermodynamic efficiency and mechanochemical coupling of F1-ATPase[J].Proc Natl Acad Sci,2011,108(44):17951
[4]Muneyuki E,Shoichi T,Tetsuaki O.Nonequilibrium energetics of a single F1-ATPase molecule[J].PRL,2010,104(19):198103
[5]Yasuda R,Noji H,Yoshida M.F1-ATPase is a highly efficient molecular motor that rotates with discrete 120steps[J].Cell,1998,93(7):1117
[6]Watanabe-Nakayama T,Toyabe S,Muneyuki E.Effect of external torque on the ATP-driven rotation of F1-ATPase[J].Biophys,2008,366(4):951
[7]Hirono-Hara Y,Ishizuka K,Noji H.Activation of pausing F1motor by external force[J].Proc Natl Acad Sci,2005,102(12):4288
[8]Spetzler D,York J,Frasch W.Microsecond timescale rotation measurements of single F1-ATPase molecules[J].Biochemistry,2006,45(10):3117
[9]包景东.经典和量子耗散系统的随机模拟方法[M].北京:科学出版社,2009
[10]Muneyuki E,Watanabe-Nakayama T,Noji H.Single molecule energetics of F1-ATPase motor[J].Biophys,2007,92(5):1806