不同配伍下防己黄芪汤中黄芪甲苷含量差异性分析
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摘要
目的:分析不同配伍下防己黄芪汤中黄芪甲苷含量差异性。方法:使用高效液相色谱-蒸发光散射(HPLC-ELSD)法检测不同组方下黄芪甲苷含有量状况,N2体积流量为2.2 mL/min,Diamonsil C18(2)色谱柱(6 mm,150×4.5 mm),漂移管温度为95℃,柱温为30℃,体积流量为1.1 m L/min,流动相乙腈-水(33∶67),并制作对照品与供试品溶液。结果:选取20 mL同一供试品溶液,色谱条件下0、2、4、6、8、10及12 h检测,得出峰面积RSD%为3.1%,说明12 h内供试品溶液的稳定性较好。选取10 mL黄芪甲苷对照品溶液,色谱条件下共进样6次,检测峰面积RSD%为3.0%,说明仪器精密度较好。制备6份供试品溶液,均进行20 m L,色谱条件检测,结构显示黄芪甲苷含量RSD%为2.9%,说明本研究方法重复性较好。精密称量6份含量已知样品,每份约重2.50g(其他药材数量不变,黄芪大约2.50g),加入2 m L含量为0.71 mg/mL的对照品溶液,依据上述供试品制备发检测加样的回收率状况,结果显示加样平均回收率为95.93%,RSD%为2.0%。制作供试品溶液,20 mL进样,检测其含量状况,不同组方防己黄芪中黄芪甲苷含量有显著改变。结论:HPLC-ELSD法检测结果可靠、准确,对检测防己黄芪汤中黄芪甲苷含量比较适宜。
Objective: To analyze the difference in the content of Astragaloside Ⅳ in different combinations.Methods: HPLC-ELSD was used to detect the content of astragaloside Ⅳ in different formulas. The volume flow rate of N2 was 2.2 m L/min, Diamonsil C18(2) column(6 mm, 150 mm×4.5 mm), the drift tube temperature was 95 °C,the column temperature was 30°C, the volumetric flow rate was 1.1 mL/min, the mobile phase acetonitrile-water(33:67), and the control and the test solution were prepared. Results: 20 m L of the same test solution was selected and chromatographic conditions were tested at 0, 2, 4, 6, 8, 10, and 12 hours. The peak area RSD%was 3.1%, indicating that the stability of the test solution was less than 12 hours. It is good. 10 m L astragaloside reference solution was selected and the sample was co-injected 6 times under the chromatographic conditions. The RSD% of the detected peak area was 3.0%, indicating that the instrument precision was better.Preparation of six samples for the test solution, were carried out 20 mL, chromatographic conditions, the structure showed astragaloside content RSD% is 2.9%, indicating that this method is better reproducibility. Weigh accurately six known samples, each weighing about 2.50 g(the amount of other herbs unchanged, about 2.50 g jaundice), add 2 m L of the reference solution containing 0.71 mg/mL, and prepare according to the above test The recovery of the sample was tested. The results showed that the average recovery rate was 95.93%, and the RSD% was 2.0%. To prepare the test solution, 20 mL injection, to detect its status, the different groups of Fangji astragalus, containing a significant change in the number of astragaloside. Conclusion: The results of HPLC-ELSD m ethod are reliable and accurate. It is more appropriate to detect the quantity of jaundice contained in Fangji Huangqi Decoction.
Objective: To analyze the difference in the content of Astragaloside Ⅳ in different combinations.Methods: HPLC-ELSD was used to detect the content of astragaloside Ⅳ in different formulas. The volume flow rate of N2 was 2.2 m L/min, Diamonsil C18(2) column(6 mm, 150 mm×4.5 mm), the drift tube temperature was 95 °C,the column temperature was 30°C, the volumetric flow rate was 1.1 mL/min, the mobile phase acetonitrile-water(33:67), and the control and the test solution were prepared. Results: 20 m L of the same test solution was selected and chromatographic conditions were tested at 0, 2, 4, 6, 8, 10, and 12 hours. The peak area RSD%was 3.1%, indicating that the stability of the test solution was less than 12 hours. It is good. 10 m L astragaloside reference solution was selected and the sample was co-injected 6 times under the chromatographic conditions. The RSD% of the detected peak area was 3.0%, indicating that the instrument precision was better.Preparation of six samples for the test solution, were carried out 20 mL, chromatographic conditions, the structure showed astragaloside content RSD% is 2.9%, indicating that this method is better reproducibility. Weigh accurately six known samples, each weighing about 2.50 g(the amount of other herbs unchanged, about 2.50 g jaundice), add 2 m L of the reference solution containing 0.71 mg/mL, and prepare according to the above test The recovery of the sample was tested. The results showed that the average recovery rate was 95.93%, and the RSD% was 2.0%. To prepare the test solution, 20 mL injection, to detect its status, the different groups of Fangji astragalus, containing a significant change in the number of astragaloside. Conclusion: The results of HPLC-ELSD m ethod are reliable and accurate. It is more appropriate to detect the quantity of jaundice contained in Fangji Huangqi Decoction.
引文
[1]伍海.玉屏风汤剂煎煮过程中黄芪甲苷含量变化及临床意义[J].四川中医,2015,3(2):93-95.
[2]李玉梅,杨辛欣,韩旭,等.川芎嗪与黄芪甲苷配伍对过氧化氢诱导人脐静脉内皮细胞氧化损伤的保护作用及机制[J].中药新药与临床药理,2017,2(1):18-23.
[3]张建平,储利胜,陶水良,等.黄芪甲苷和三七总皂苷配伍对脑缺血再灌注损伤大鼠海马NSCs的保护作用[J].中成药,2017,39(11):2372-2376.
[4]李玉梅,杨辛欣,韩旭,等.川芎嗪与黄芪甲苷配伍对人脐静脉内皮细胞血管生成的作用及机制探讨[J].中草药,2017,48(4):722-727.
[5]魏春华.当归补血汤中当归与黄芪不同比例搭配对其化学成分的影响[J].四川中医,2015,7(6):51-53.
[6]张建伟,庞帼敏,麦国荣,等.黄芪白术配伍前后黄芪化学成分分析研究[J].世界中医药,2017,12(12):3154-3157.
[7]刘耀,石英,刘职瑞,等.黄芪多糖与黄芪甲苷配伍对辐射损伤模型小鼠的保护作用[J].中国药房,2014,5(3):211-214.
[8]李静娴,杨筱倩,唐标,等.黄芪甲苷和三七总皂苷配伍抗大鼠脑缺血再灌注损伤及其药动学的研究[J].中国中药杂志,2017,42(19):3786-3794.
[9]黄小平,欧阳国,丁煌,等.黄芪甲苷与三七有效成分配伍对小鼠脑缺血再灌注后神经细胞凋亡和内质网应激的影响[J].中草药,2015,46(15):2257-2264.
[10]周惠芬,何昱,张宇燕,等.黄芪川芎主要有效成分配伍的转运研究[J].中华中医药学刊,2015,7(4):868-871.
[11]刘晓丹,邓常清.黄芪甲苷和三七总皂苷中主要有效成分抗PC12细胞氧化损伤的配伍研究[J].湖南中医药大学学报,2012,32(1):8-12.
[12]丁煌,李静娴,唐标,等.黄芪甲苷与人参皂苷Rg1配伍抗PC12细胞氧糖剥夺/复糖复氧后自噬性损伤相互作用的研究[J].中国药理学通报,2017,33(2):235-243.
[13]崔运浩,初杰,范颖,等.黄芪甲苷、毛蕊异黄酮及其配伍对化疗性骨髓抑制小鼠骨髓干细胞JAK2/STAT5信号转导通路的影响[J].中华中医药学刊,2016,9(7):1576-1580.
[14]许航,丁一,刘文星,等.黄芪甲苷和羟基红花黄色素A配伍抗脑缺血再灌注损伤的协同作用及机制研究[J].中华神经外科疾病研究杂志,2017,16(5):427-431.
[15]黄小平,王蓓,邱咏园,等.黄芪甲苷和三七的主要有效成分配伍对小鼠脑缺血再灌注后脑组织能量代谢的影响[J].中草药,2014,45(2):220-226.
[16]陈萌,李爱平,李科,等.防己黄芪汤HPLC-UV/ELSD指纹图谱研究[J].中草药,2017,48(13):2653-2659.
[2]李玉梅,杨辛欣,韩旭,等.川芎嗪与黄芪甲苷配伍对过氧化氢诱导人脐静脉内皮细胞氧化损伤的保护作用及机制[J].中药新药与临床药理,2017,2(1):18-23.
[3]张建平,储利胜,陶水良,等.黄芪甲苷和三七总皂苷配伍对脑缺血再灌注损伤大鼠海马NSCs的保护作用[J].中成药,2017,39(11):2372-2376.
[4]李玉梅,杨辛欣,韩旭,等.川芎嗪与黄芪甲苷配伍对人脐静脉内皮细胞血管生成的作用及机制探讨[J].中草药,2017,48(4):722-727.
[5]魏春华.当归补血汤中当归与黄芪不同比例搭配对其化学成分的影响[J].四川中医,2015,7(6):51-53.
[6]张建伟,庞帼敏,麦国荣,等.黄芪白术配伍前后黄芪化学成分分析研究[J].世界中医药,2017,12(12):3154-3157.
[7]刘耀,石英,刘职瑞,等.黄芪多糖与黄芪甲苷配伍对辐射损伤模型小鼠的保护作用[J].中国药房,2014,5(3):211-214.
[8]李静娴,杨筱倩,唐标,等.黄芪甲苷和三七总皂苷配伍抗大鼠脑缺血再灌注损伤及其药动学的研究[J].中国中药杂志,2017,42(19):3786-3794.
[9]黄小平,欧阳国,丁煌,等.黄芪甲苷与三七有效成分配伍对小鼠脑缺血再灌注后神经细胞凋亡和内质网应激的影响[J].中草药,2015,46(15):2257-2264.
[10]周惠芬,何昱,张宇燕,等.黄芪川芎主要有效成分配伍的转运研究[J].中华中医药学刊,2015,7(4):868-871.
[11]刘晓丹,邓常清.黄芪甲苷和三七总皂苷中主要有效成分抗PC12细胞氧化损伤的配伍研究[J].湖南中医药大学学报,2012,32(1):8-12.
[12]丁煌,李静娴,唐标,等.黄芪甲苷与人参皂苷Rg1配伍抗PC12细胞氧糖剥夺/复糖复氧后自噬性损伤相互作用的研究[J].中国药理学通报,2017,33(2):235-243.
[13]崔运浩,初杰,范颖,等.黄芪甲苷、毛蕊异黄酮及其配伍对化疗性骨髓抑制小鼠骨髓干细胞JAK2/STAT5信号转导通路的影响[J].中华中医药学刊,2016,9(7):1576-1580.
[14]许航,丁一,刘文星,等.黄芪甲苷和羟基红花黄色素A配伍抗脑缺血再灌注损伤的协同作用及机制研究[J].中华神经外科疾病研究杂志,2017,16(5):427-431.
[15]黄小平,王蓓,邱咏园,等.黄芪甲苷和三七的主要有效成分配伍对小鼠脑缺血再灌注后脑组织能量代谢的影响[J].中草药,2014,45(2):220-226.
[16]陈萌,李爱平,李科,等.防己黄芪汤HPLC-UV/ELSD指纹图谱研究[J].中草药,2017,48(13):2653-2659.