脂质体药物微粒的共焦显微拉曼光谱鉴别
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摘要
雷公藤红素为雷公藤的根皮当中提取而来的抗癌活性成分,利用共焦显微拉曼光谱仪无损快速地检测了脂质体包裹的雷公藤红素药物微粒,并与空白脂质体微粒、单纯雷公藤红素的拉曼光谱进行对比,可以看出,药物经脂质体包裹后,仍可以在光谱中进行清晰识别的情况,为后续研究奠定基础.
Celastrol was a kind of anti-tumor active ingredient extracted from tripterygium wilfordii root and cortex,liposome was relatively mature as drug carrier of application,this paper used the confocal micro Raman spectroscopy non-destructive to detect celastrol packaged by liposome quickly and scathelessly,The Raman spectrum with the blank liposome 's and celastrol's had been compared. It can be seen from these Raman spectra,liposomal celastrol still can be clearly identify in the spectrum,which had provided some ways and means for future research of celastrol.
Celastrol was a kind of anti-tumor active ingredient extracted from tripterygium wilfordii root and cortex,liposome was relatively mature as drug carrier of application,this paper used the confocal micro Raman spectroscopy non-destructive to detect celastrol packaged by liposome quickly and scathelessly,The Raman spectrum with the blank liposome 's and celastrol's had been compared. It can be seen from these Raman spectra,liposomal celastrol still can be clearly identify in the spectrum,which had provided some ways and means for future research of celastrol.
引文
[1]黄煜伦.雷公藤红素纳米脂质体的制备及抗胶质瘤的实验研究[D].苏州:苏州大学,2009.
[2]潘东宁.脂质体辅料PEG—L—Chol的合成研究[D].石家庄:河北科技大学,2011:1-2.
[3]任玉芹.脂质体作为药物载体的研究概述[J]. 2005,17(2):9-11.
[4]杨宝,杨四涛,王磊.脂质体的制备技术及研究进展[J].医药前沿,2013(5):96-98.
[5]陶站华,姚辉璐,王桂文,等.利用拉曼光谱分析顺铂诱导的胃癌细胞凋亡[J].光谱学与光谱分析,2009,29(9):2441-2445.
[6] TAO Z H,WANG G W,XU X D,et al. Monitoring and rapid quantifi-cation of total carotenoids in Rhodotorula glutiniscells using laser tweezers Raman spectroscopy[J]. FEMS Microbiology Letters,2011,314(1):42-48.
[7] BUIJTELS P C,WILLEMSE-ERIX H F,PETIT P L,et al.Rapid identification of mycobacteria by Raman spectroscopy[J].Journal of Clinical Microbiology,2008,46(3):961-965.
[8] PAPAIOANNOU E H,LIAKOPOULOU-KYRIAKIDES M,CHRISTOFILOS D,et al. Raman spectroscopy for intracellular monitoring of carotenoid in Blakeslea trispora[J]. Applied Biochemistry and Biotechnology,2009,159(2):478-487.
[9] PALCHAUDHURI S,REHSE S J,HAMASHA K,et al. Raman spectroscopy of xylitol uptake and metabolism in Grampositive and Gram-negative bacteria[J]. Applied and Environment Microbiology,2011,77(1):131-137.
[10]孙美娟,刘军贤,王雪,等.拉曼光谱技术在微生物学中的应用[J].生物技术通报,2012(10):63-67.
[11]钟会清,张武,侯雨晴,等.激光共焦显微拉曼光谱技术在人舌鳞癌细胞检测中的应用[J].中国医学物理学杂志,2017,34(7):753-756.
[12]张阳德.纳米药物学[M].北京:化学工业出版社,2005. 10.
[13] THRALL J H. Nanotechnology and medicine[J]. Radiology,2004,230(2):315-318.
[14]袁菱,童德银.雷公藤红素及其制剂的抗肿瘤研究进展[J].中国医院药学杂志,2016,36(14):1224-1229.
[15] HUANG W,WU S S. Study of both fingerprint and high wavenumber Raman spectroscopy of pathological nanopharyngeal tissues[J]. Journal of Raman Spectroscopy,2015,46:537-544.
[2]潘东宁.脂质体辅料PEG—L—Chol的合成研究[D].石家庄:河北科技大学,2011:1-2.
[3]任玉芹.脂质体作为药物载体的研究概述[J]. 2005,17(2):9-11.
[4]杨宝,杨四涛,王磊.脂质体的制备技术及研究进展[J].医药前沿,2013(5):96-98.
[5]陶站华,姚辉璐,王桂文,等.利用拉曼光谱分析顺铂诱导的胃癌细胞凋亡[J].光谱学与光谱分析,2009,29(9):2441-2445.
[6] TAO Z H,WANG G W,XU X D,et al. Monitoring and rapid quantifi-cation of total carotenoids in Rhodotorula glutiniscells using laser tweezers Raman spectroscopy[J]. FEMS Microbiology Letters,2011,314(1):42-48.
[7] BUIJTELS P C,WILLEMSE-ERIX H F,PETIT P L,et al.Rapid identification of mycobacteria by Raman spectroscopy[J].Journal of Clinical Microbiology,2008,46(3):961-965.
[8] PAPAIOANNOU E H,LIAKOPOULOU-KYRIAKIDES M,CHRISTOFILOS D,et al. Raman spectroscopy for intracellular monitoring of carotenoid in Blakeslea trispora[J]. Applied Biochemistry and Biotechnology,2009,159(2):478-487.
[9] PALCHAUDHURI S,REHSE S J,HAMASHA K,et al. Raman spectroscopy of xylitol uptake and metabolism in Grampositive and Gram-negative bacteria[J]. Applied and Environment Microbiology,2011,77(1):131-137.
[10]孙美娟,刘军贤,王雪,等.拉曼光谱技术在微生物学中的应用[J].生物技术通报,2012(10):63-67.
[11]钟会清,张武,侯雨晴,等.激光共焦显微拉曼光谱技术在人舌鳞癌细胞检测中的应用[J].中国医学物理学杂志,2017,34(7):753-756.
[12]张阳德.纳米药物学[M].北京:化学工业出版社,2005. 10.
[13] THRALL J H. Nanotechnology and medicine[J]. Radiology,2004,230(2):315-318.
[14]袁菱,童德银.雷公藤红素及其制剂的抗肿瘤研究进展[J].中国医院药学杂志,2016,36(14):1224-1229.
[15] HUANG W,WU S S. Study of both fingerprint and high wavenumber Raman spectroscopy of pathological nanopharyngeal tissues[J]. Journal of Raman Spectroscopy,2015,46:537-544.