海南习用药材无柄灵芝质量标准研究
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摘要
为建立海南习用药材无柄灵芝的质量标准,依据《中国药典》2015年版中药质量标准研制的要求,对无柄灵芝药材的性状、鉴别、检查、浸出物、灰分、含量测定等项目进行研究。本研究明确了无柄灵芝药材的性状和显微结构特征,建立了专属的薄层鉴别方法;规定无柄灵芝多糖含量不得低于0. 40%、三萜与甾醇含量不得低于0. 40%、水分不得超过18. 0%、总灰分不得超过4. 0%、浸出物不得低于2. 0%,为无柄灵芝药材质量控制及资源开发利用提供科学依据。
To establish the quality standard of Ganoderma Sessilia,a traditional medicinal material in Hainan,the properties,identification,inspection,extract,ash and content determination of Ganoderma Sessilia were studied according to the requirement of the quality standard of Traditional Chinese Medicine of the Chinese Pharmacopoeia 2015 edition. The properties and microstructural characteristics of Ganoderma Sessilia were identified,and the exclusive thin layer chromatography identification method of Ganoderma Sessilia was established. It is stipulated that the content of polysaccharide,triterpenoid and sterol in Ganoderma Sessilia should not be less than 0. 40%,water content should not be less than 18. 0%,total ash content should not be more than 4. 0%,and extracts should not be less than 2. 0%,which could provide scientific basis for the quality control,resource development and utilization of Ganoderma Sessilia.
To establish the quality standard of Ganoderma Sessilia,a traditional medicinal material in Hainan,the properties,identification,inspection,extract,ash and content determination of Ganoderma Sessilia were studied according to the requirement of the quality standard of Traditional Chinese Medicine of the Chinese Pharmacopoeia 2015 edition. The properties and microstructural characteristics of Ganoderma Sessilia were identified,and the exclusive thin layer chromatography identification method of Ganoderma Sessilia was established. It is stipulated that the content of polysaccharide,triterpenoid and sterol in Ganoderma Sessilia should not be less than 0. 40%,water content should not be less than 18. 0%,total ash content should not be more than 4. 0%,and extracts should not be less than 2. 0%,which could provide scientific basis for the quality control,resource development and utilization of Ganoderma Sessilia.
引文
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[2]Wang G Q.Compilation of Chinese herbal medicine[M].Beijing:People's Medical Publishing House,2014:327-329.王国强.全国中草药汇编[M].北京:人民卫生出版社,2014:327-329.
[3]Li B Y,Wu X L,Song B,et al.Distribution of the me-dicinal Ganoderma ssp.in Hainan[J].Guangdong Ag-ric Sci,2013,40(3):171-172.李碧英,吴兴亮,宋斌,等.海南岛药用灵芝真菌资源及分布[J].广东农业科学,2013,40(3):171-172.
[4]Zhao J D.Studies on the taxonomy of ganoder-mataceae in China[J].Acta Mycologica Sinica,1989,8(1):25-34,83.赵继鼎.中国灵芝科的分类研究Ⅺ.灵芝亚属灵芝组[J].真菌学报,1989,8(1):25-34,83.
[5]Zhao J D,Zhang X Q.Chinese fungi[M].Beijing:Sci-ence Press,2000:37-38,66-67.赵继鼎,张小青.中国真菌志[M].北京:科学出版社,2000:37-38,66-67.
[6]Lan N N,Ma Q Y,Huang S Z,et al.Chemical con-stituents from fruiting bodies of Ganoderma daiqingshanense[J].Chin Tradit Herb Drugs,2017,48(3):437-442.兰宁宁,马青云,黄圣卓,等.大青山灵芝的化学成分研究[J].中草药,2017,48(3):437-442.
[7]Liu L Y,Kang J,Wu C H,et al.Triterpenoids from Ganoderma theaecolum[J].China J Chin Mater Med,2016,41(6):1075-1080.刘莉莹,康洁,吴长辉,等.茶病灵芝中三萜类成分研究[J].中国中药杂志,2016,41(6):1075-1080.
[8]Chen R Y,Yu D Q.Research progress on chemical constituents of triterpenes from Ganoderma lucidum[J].Acta Pharmaceutica Sinica,1990,25(12):940-953.陈若芸,于德泉.灵芝三萜化学成分研究进展[J].药学学报,1990,25(12):940-953.
[9]Liu L Y.Studies on the chemical constituents and bioac-tivities of the fruiting bodies of Ganoderma theaecolum,Ganoderma sessile and Ganoderma mastoporum[D].Peking Union Medical College,2017:1-39.刘莉莹.茶病灵芝、树灵芝和无柄紫灵芝化学成分及生物活性研究[D].北京协和医学院,2017:1-39.
[10]Li L R,Zhang H M,Ni C,et al.Discussion on the di-agnosis and treatment of bronchial asthma[J].Tradition-al Chinese Medicine Journal,2012,11(3):8-15.李玲孺,张惠敏,倪诚,等.关于支气管哮喘诊疗思路的探讨[J].中医药通报,2012,11(3):8-15.
[11]Nanjing University Of Chinese Medicine.Chinese medi-cine dictionary[M].Shanghai:Shanghai Science and Technology Press,2017:1649-1653.南京中医药大学.中药大辞典[M].上海:上海科学技术出版社,2017:1649-1653.
[12]Chinese Pharmacopoeia Commission.Pharmacopoeia of People′s Republic of China(Part 4)[M].Beijing:Chi-na Medical Science Press,2015:30.国家药典委员会.中华人民共和国药典(四部)[M].北京:中国医药科技出版社,2015:30.
[13]Dai Z F,Peng M.Hainan Chinese Medicine Resources List[M].China Agriculture Press,2009:3-4.代正福,彭明.海南中药资源名录[M].北京:中国农业出版社,2009:3-4.