鹅肝酶解物对酒精性脂肪肝的改善作用
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摘要
为探索鹅肝酶解物对酒精性脂肪肝的防治效果,以6周龄SD大鼠为研究对象,将其随机分为对照组、模型组,以及鹅肝酶解物高(GLH-H)、低剂量处理组(GLH-L),其中对照组以Lieber-DeCarli液体对照饲料喂养,其他各组以Lieber-DeCarli乙醇液体饲料喂养;GLH-H、GLH-L处理组分别给药600、200 mg·kg~(-1) BW(体质量)GLH进行干预,对照组和模型组分别给予相同体积的生理盐水灌胃,试验共持续5周。试验结束后测定各试验组大鼠的体质量、肝脏指数、血清中尿素氮、总胆固醇、甘油三酯及炎症因子,分析大鼠肠道微生物的组成,并对各试验组大鼠肝脏病理切片进行观察。结果表明,GLH处理明显降低了酒精性脂肪肝大鼠的肝脏指数(P<0.05),改善了酒精性脂肪肝大鼠肝脏脂肪变性程度,显著降低了大鼠血清中的尿素氮、总胆固醇、甘油三酯及炎症水平(P<0.05)。高通量测序发现,GLH可改善大鼠肠道微生物紊乱的现象。综上,GLH对酒精性脂肪肝的发生具有一定的预防作用。本研究结果为酒精性脂肪肝治疗药物的研究提供了新思路。
This study aimed to explore the preventive effects of goose liver hydrolysate(GLH) on the alcoholic fatty liver(AFL) disease in rats. 6-week-old SD rats were randomly divided into 4 groups with different supplements in Lieber-DeCarli liquid basal diet: control group(normal saline), model group(normal saline + ethanol), GLH-H(600 mg·kg~(-1) BW GLH + ethanol) and GLH-L(200 mg·kg~(-1) BW GLH + ethanol). The experiment period lasted for 5 weeks. And then for each rat, the body weight, liver index, serum urea nitrogen, total cholesterol, triglyceride and inflammatory factors were measured, the composition of the intestinal microflora was analyzed, and the liver pathological section was prepared and observed. The results showed that GLH treatment improved the degree of hepatic steatosis and significantly reduced the liver index, serum nitrogen, total cholesterol, triglyceride and serum inflammation levels in GLH-H and GLH-L rats, compared to AM rats(P<0.05). At the same time, high-throughput sequencing revealed that GLH could also ameliorate the disorder of intestinal microflora caused by AFL. The results indicate that GLH has preventive effects on the occurrence of AFL disease. This this study would provide new ideas for the exploration of drugs for AFL disease.
This study aimed to explore the preventive effects of goose liver hydrolysate(GLH) on the alcoholic fatty liver(AFL) disease in rats. 6-week-old SD rats were randomly divided into 4 groups with different supplements in Lieber-DeCarli liquid basal diet: control group(normal saline), model group(normal saline + ethanol), GLH-H(600 mg·kg~(-1) BW GLH + ethanol) and GLH-L(200 mg·kg~(-1) BW GLH + ethanol). The experiment period lasted for 5 weeks. And then for each rat, the body weight, liver index, serum urea nitrogen, total cholesterol, triglyceride and inflammatory factors were measured, the composition of the intestinal microflora was analyzed, and the liver pathological section was prepared and observed. The results showed that GLH treatment improved the degree of hepatic steatosis and significantly reduced the liver index, serum nitrogen, total cholesterol, triglyceride and serum inflammation levels in GLH-H and GLH-L rats, compared to AM rats(P<0.05). At the same time, high-throughput sequencing revealed that GLH could also ameliorate the disorder of intestinal microflora caused by AFL. The results indicate that GLH has preventive effects on the occurrence of AFL disease. This this study would provide new ideas for the exploration of drugs for AFL disease.
引文
[1] 刘祥兰,徐颖,张钰泉,钱燕娟,吴耀萍,李琴韵.猪苓多糖降血脂抗大鼠酒精性脂肪肝的药理实验研究[J].中成药,2013,35(8):1760-1764
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[16] 昭日格图,白朝鲁门,博格日勒图,娜日苏,领小.荜茇宁对小鼠酒精性脂肪肝的预防作用[J].食品科学,2014,35(13):232-235
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[20] Sun F,Xie M L,Zhu L J,Xue J,Gu Z L.Inhibitory effect of osthole on alcohol-induced fatty liver in mice[J].Digestive & Liver Disease,2009,41(2):127-133
[21] 梁卫,吴承玉,梁涛.清肝利湿解酒汤对酒精性脂肪肝大鼠肝损伤保护作用研究[J].中国实验方剂学杂志,2013,19(4):163-167
[22] Keppler D.Uptake and efflux transporters for conjugates in human hepatocytes[J].Methods in Enzymology,2005,400:531-542
[23] Lu Y,Ward S C,Cederbaum A Ⅰ.Nicotine enhances ethanol-induced fat accumulation and collagen deposition but not inflammation in mouse liver[J].Alcohol,2013,47(5):353-357
[24] Zhang W,Hong R,Tian T.Silymarin′s protective effects andpossible mechanisms on alcoholic fatty liver for rats[J].Biomolecules & Therapeutics,2013,21(4):264-269
[25] Park H Y,Park Y,Lee Y,Noh S K,Sung E,Choi Ⅰ.Effect of oral administration of water-soluble extract from citrus peel (Citrus unshiu) on suppressing alcohol-induced fatty liver in rats[J].Food Chemistry,2012,130(3):598-604
[26] 胡成穆,曹琦,李俊.酒精性脂肪肝脂质代谢研究进展[J].安徽医药,2012,16(8):1045-1047
[27] Zhao Y Y,Yang R,Xiao M,Guan M J,Zhao N,Zeng T.Kupffer cells activation promoted binge drinking-induced fatty liver by activating lipolysis in white adipose tissues[J].Toxicology,2017,390:53-60
[28] 朱莹莹,李春保,周光宏.饮食、肠道微生物与健康的关系研究进展[J].食品科学,2015,36(15):234-239
[29] Dubinkina Ⅴ B,Tyakht A Ⅴ,Odintsova Ⅴ Y,Yarygin K S,Kovarsky B A,Pavlenko A Ⅴ,Ischenko D S.Links of gut microbiota composition with alcohol dependence syndrome and alcoholic liver disease[J].Microbiome,2017,5(1):141-153
[30] Tilg H,Grander C,Moschen A R.How does the microbiome affect liver disease?[J].Clinical Liver Disease,2016,8(5):123-126
[31] Henaomejia J,Elinav E,Thaiss C A,Flavell R A.The intestinal microbiota in chronic liver disease[J].Advances in Immunology,2013,117:73-97
[32] Szabo G,Bala S,Petrasek J,Gattu A.Gut-liver axis and sensing microbes[J].Digestive Diseases,2011,28(6):737-744
[33] Mutlu E A,Gillevet P M,Rangwala H.Colonic microbiome is altered in alcoholism[J].American Journal of Physiology - Gastrointestinal and Liver Physiology,2012,302(9):966-978
[2] Peng Z,Borea P A,Varani K,Wilder T,Yee H,Luis C,Blackburn M R,Azzena G,Resta G,Cronstein B N.Adenosine signaling contributes to ethanol-induced fatty liver in mice[J].Journal of Clinical Investigation,2009,119(3):582-594
[3] Li Y,Ding W X.Adipose tissue autophagy and homeostasis in alcohol-induced liver injury[J].Liver Research,2017,1(1):54-62
[4] Altamirano J Bataller R.Alcoholic liver disease:Pathogenesis and new targets for therapy[J].Nature Reviews.Gastroenterology & Hepatology,2011,8(9):491-501
[5] 李国春.赶黄草对大鼠酒精性脂肪肝的保护作用及机制[J].中国老年学杂志,2015,35(14):3845-3847
[6] Zhang M,Wang C,Wang C,Zhao H,Zhao C,Chen Y,Wang Y,McClain C,Feng W.Enhanced AMPK phosphorylation contributes to the beneficial effects of Lactobacillus rhamnosus GG supernatant on chronic-alcohol-induced fatty liver disease[J].The Journal of Nutritional Biochemistry,2015,26(4):337-344
[7] Ding R B,Tian K,Huang L L,He C W,Jiang Y,Wang Y T,Wan J B.Herbal medicines for the prevention of alcoholic liver disease:A review[J].Journal of Ethnopharmacology,2012,144(3):457-465
[8] 韩文宝.我国鹅养殖业的现状及未来发展方向[J].家禽科学,2017(8):42-44
[9] 鞠美玲,周晓燕.不同烹调方法对普通鹅肝中部分常量营养素的影响[J].扬州大学烹饪学报,2012,29(1):37-39
[10] 王立,卞欢,吴海虹,邹烨,王道营,徐为民.超声辅助酶解鹅肝蛋白的动力学和热力学及酶解物抗氧化性能[J].江苏农业学报,2017,33(6):1395-1401
[11] Yang K T,Lin C,Liu C W,Chen Y C.Effects of chicken-liver hydrolysates on lipid metabolism in a high-fat diet[J].Food Chemistry,2014,160(11):148-156
[12] Chou C H,Wang S Y,Lin Y T,Chen Y C.Antioxidant activities of chicken liver hydrolysates by pepsin treatment[J].International Journal of Food Science & Technology,2014,49(7):1654-1662
[13] 舒沿沿,胡冰雪,潘道东,曾小群,孙杨嬴,吴振,曹锦轩.发酵鹅肝肽的分离纯化及其抗氧化特性研究[J].食品工业科技,2016,37(24):99-104
[14] Zhao X,Wang L,Zhang H,Zhang D,Zhang Z,Zhang J.Protective effect of artemisinin on chronic alcohol induced-liver damage in mice[J].Environmental Toxicology & Pharmacology,2017,52:221-226
[15] 王宗伟,牟晓玲,杨国伟,李佶恺,刘大森.日粮营养素水平对东北肉鹅生长性能及血液生化指标的影响(1～28日龄)[J].核农学报,2009,23(5):891-897
[16] 昭日格图,白朝鲁门,博格日勒图,娜日苏,领小.荜茇宁对小鼠酒精性脂肪肝的预防作用[J].食品科学,2014,35(13):232-235
[17] Ajmo J M,Liang X,Rogers C Q,Pennock B,You M.Resveratrol alleviates alcoholic fatty liver in mice[J].American Jourrnal of Physiology-Gastrointestinal and Liver Physiology,2008,295(4):833-842
[18] Bai T,Yang Y,Yao Y L,Sun P,Lian L H,Wu Y L.Betulin alleviated ethanol-induced alcoholic liver injury via SIRT1/AMPK signaling pathway[J].Pharmacological Research,2016,105:1-12
[19] Kim M J,Sim M O,Lee H Ⅰ,Ham J R,Seo K,Lee M K.Dietary umbelliferone attenuates alcohol-induced fatty liver via regulation of PPARα and SREBP-1c in rats[J].Alcohol,2014,48(7):707-715
[20] Sun F,Xie M L,Zhu L J,Xue J,Gu Z L.Inhibitory effect of osthole on alcohol-induced fatty liver in mice[J].Digestive & Liver Disease,2009,41(2):127-133
[21] 梁卫,吴承玉,梁涛.清肝利湿解酒汤对酒精性脂肪肝大鼠肝损伤保护作用研究[J].中国实验方剂学杂志,2013,19(4):163-167
[22] Keppler D.Uptake and efflux transporters for conjugates in human hepatocytes[J].Methods in Enzymology,2005,400:531-542
[23] Lu Y,Ward S C,Cederbaum A Ⅰ.Nicotine enhances ethanol-induced fat accumulation and collagen deposition but not inflammation in mouse liver[J].Alcohol,2013,47(5):353-357
[24] Zhang W,Hong R,Tian T.Silymarin′s protective effects andpossible mechanisms on alcoholic fatty liver for rats[J].Biomolecules & Therapeutics,2013,21(4):264-269
[25] Park H Y,Park Y,Lee Y,Noh S K,Sung E,Choi Ⅰ.Effect of oral administration of water-soluble extract from citrus peel (Citrus unshiu) on suppressing alcohol-induced fatty liver in rats[J].Food Chemistry,2012,130(3):598-604
[26] 胡成穆,曹琦,李俊.酒精性脂肪肝脂质代谢研究进展[J].安徽医药,2012,16(8):1045-1047
[27] Zhao Y Y,Yang R,Xiao M,Guan M J,Zhao N,Zeng T.Kupffer cells activation promoted binge drinking-induced fatty liver by activating lipolysis in white adipose tissues[J].Toxicology,2017,390:53-60
[28] 朱莹莹,李春保,周光宏.饮食、肠道微生物与健康的关系研究进展[J].食品科学,2015,36(15):234-239
[29] Dubinkina Ⅴ B,Tyakht A Ⅴ,Odintsova Ⅴ Y,Yarygin K S,Kovarsky B A,Pavlenko A Ⅴ,Ischenko D S.Links of gut microbiota composition with alcohol dependence syndrome and alcoholic liver disease[J].Microbiome,2017,5(1):141-153
[30] Tilg H,Grander C,Moschen A R.How does the microbiome affect liver disease?[J].Clinical Liver Disease,2016,8(5):123-126
[31] Henaomejia J,Elinav E,Thaiss C A,Flavell R A.The intestinal microbiota in chronic liver disease[J].Advances in Immunology,2013,117:73-97
[32] Szabo G,Bala S,Petrasek J,Gattu A.Gut-liver axis and sensing microbes[J].Digestive Diseases,2011,28(6):737-744
[33] Mutlu E A,Gillevet P M,Rangwala H.Colonic microbiome is altered in alcoholism[J].American Journal of Physiology - Gastrointestinal and Liver Physiology,2012,302(9):966-978