桃儿七不同部位2种木脂素含量的动态研究
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摘要
采用超声提取和HPLC测定栽培植株与野生植株桃儿七不同部位中4'-去甲基鬼臼毒素和鬼臼毒素的含量,结果表明,栽培植株各部位4'-去甲基鬼臼毒素含量依次为叶柄>根,叶中未检测到此物质,野生植株不同部位4'-去甲基鬼臼毒素含量依次为须根>茎>根状茎>叶,果实中未检测到此物质;栽培植株和野生植株不同部位鬼臼毒素含量均为根>茎>叶(>果实);桃儿七栽培植株各部位2种木脂素含量随季节变化呈W型,以7月份采收时含量最高。
The contents of two lignans,namely 4'-demethylpodophyllotoxin and podophyllotoxin in cultivated and wild Sinopodophyllum hexandrum plants were extracted by ultrasonicaction and determined by HPLC. According to the result showed,the order of parts of cultivated plants containing 4'-demethylpodophyllotoxin from high to low is as follows: stem > root,no 4'-demethypodophyllotoxin was detected in leaves of cultivated plants; The order of parts of wild plants 4'-demethylpodophyllotoxin from high to low is as follows: lateral root > petiole > rhizome > leaf,no 4'-demethypodophyllotoxin was detected in fruit. The order of parts of cultivated and wild S. hexandrum containing podophyllotoxin from high to low is as follows: root > petiole > leaf( > fruit). Both of the lignan contents in different parts of cultivated plant varied in a " W" curve with the changes in seasons,with the highest content in July.
The contents of two lignans,namely 4'-demethylpodophyllotoxin and podophyllotoxin in cultivated and wild Sinopodophyllum hexandrum plants were extracted by ultrasonicaction and determined by HPLC. According to the result showed,the order of parts of cultivated plants containing 4'-demethylpodophyllotoxin from high to low is as follows: stem > root,no 4'-demethypodophyllotoxin was detected in leaves of cultivated plants; The order of parts of wild plants 4'-demethylpodophyllotoxin from high to low is as follows: lateral root > petiole > rhizome > leaf,no 4'-demethypodophyllotoxin was detected in fruit. The order of parts of cultivated and wild S. hexandrum containing podophyllotoxin from high to low is as follows: root > petiole > leaf( > fruit). Both of the lignan contents in different parts of cultivated plant varied in a " W" curve with the changes in seasons,with the highest content in July.
引文
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[2]中国科学院中国植物志编辑委员会.中国植物志[M].北京:科学出版社,2001:249.
[3]强巴赤烈,嘎玛曲佩,占堆,等.中华本草·藏药卷[M].上海:上海科学技术出版社,2002.
[4]袁菊丽.太白七药桃儿七研究进展[J].辽宁中医药大学学报,2011,13(5):95.
[5]贾敏如,李星炜.中国民族药志要[M].北京:中国医药科技出版社,2005:568.
[6]虞泓.珍稀植物桃儿七[J].植物杂志,1999(3):6.
[7]周汉荣.中药资源学[M].北京:中国医药科技出版社,1993:57.
[8]Wang P,Liu Y,Chen T,et al.One-step separation and purification of three lignans and one flavonol from sinopodophyllum emodi by medium-pressure liquid chromatography and high-speed counter-current chromatography[J].Phytochem Anal,2013,24(6):603.
[9]Zhao C Q,Huang J,Nagatsu A,et al.Two new podophyllotoxin glucosides from Sinopodophyllum emodi(Wall.)Ying[J].Chem Pharm Bull,2001,49(6):773.
[10]Sun Y J,Li Z L,Chen H,et al.Three new cytotoxic aryltetralin lignans from Sinopodophyllum emodi[J].Bioorg Med Chem Lett,2011,21(12):3794.
[11]Li M F,Li W,Yang D L,et al.Relationship between podophyllotoxin accumulation and soil nutrients and the influence of Fe2+and Mn2+on podophyllotoxin biosynthesis in Podophyllum hexandrum tissue culture[J].Plant Physiol Bioch,2013,71:96.
[12]Vanisree M,Lee C Y,Lo S F,et al.Studies on the production of some important secondary metabolites from medicinal plants by plant tissue cultures[J].Bot Bull Acad Sin,2004,45(1):1.
[13]Alam E A.In vitro cultures for the production of some anticancer agents(review article)[J].Life Sci,2013,10(9s):297.
[14]Rajesh M,Sivanandhan G,Subramanyam K,et al.Establishment of somatic embryogenesis and podophyllotoxin production in liquid shake cultures of Podophyllum hexandrum Royle[J].Ind Crop Prod,2014,60:66.
[15]马绍宾,胡志浩,李俊.桃儿七生化生态适应的初步研究[J].生态学杂志,1997,16(3):67.
[16]白亚民,徐建中.桃儿七中鬼臼脂素的高效液相色谱法分离与含量测定[J].中药通报,1988,13(5):39.
[17]杜微.桃儿七种子成分分析和不同组织鬼臼毒素含量的比较研究[D].兰州:甘肃农业大学,2010.
[18]熊文勇,魏朔南,岳明.HPLC分析桃儿七中鬼臼毒素的含量[J].中成药,2010(5):875.
[19]黄坤,蒋伟,赵纪峰,等.濒危药用植物桃儿七中鬼臼毒素和总木脂素含量测定[J].中国中药杂志,2012,37(10):1360.
[20]马绍宾,徐正尧,胡志浩.桃儿七繁殖生物学研究[J].西北植物学报,1997,17(1):49.
[21]赵则海,曹建国,李庆勇,等.黑龙江省西部乌拉尔甘草总黄酮含量的动态变化研究[J].植物研究,2004(2):235.
[22]张康健,马希汉,马梅,等.杜仲叶次生代谢物生长积累动态的研究[J].林业科学,1999(2):18.
[23]曹建国,赵则海,李庆勇,等.刺五加丁香苷和总黄酮含量及其季节动态[J].植物学通报,2006(3):269.
[24]高蓉,狄旭东,杨振德,等.4种鬼臼毒素类似物对分月扇舟蛾的生物活性研究[J].农药,2004,43(9):424.