转录组分析挖掘油菜耐旱基因
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摘要
本研究选用耐旱性好的甘蓝型油菜品系YAU200908为材料,通过对干旱胁迫下根和叶的转录组进行分析,发现干旱胁迫下根中和叶中差异表达Unigene分别为9 419个和13 230个,其中上调表达的略多于下调表达的。并对差异表达基因(differentially expressed genes, DEGs)进行COG、GO等功能分类和生物信息学分析,挖掘出20个unigene参与了YAU200908抗旱反应,它们分别是转录因子4个、渗透调节4个、蒸腾作用调节4个、活性氧清除2个及ABA水平调控6个。本研究对油菜耐旱分子机制的解析及耐旱品种选育提供了一定的依据。
In this study, Brassica napus var. YAU200908 with good drought-tolerance was used as materials. By analyzing the transcriptomes of roots and leaves under drought stress, we found that there were 9 419 Unigenes and13 230 Unigenes respectively expressed in roots and leaves under drought stress, of which the up-regulated expression was slightly more than the down-regulated expression. The differentially expressed genes(DEGs) have been analyzed in COG database and GO database, and 20 unigenes related to drought-tolerance in YAU200908 were discovered, such as 4 unigenes for transcription factor, 4 unigenes for osmotic adjustment, 4 unigenes for transpiration regulation, 2 unigenes for the removal of reactive oxygen and 6 unigenes for ABA level regulation.The research would provide a basis for illuminating molecular mechanism of rapeseed drought-tolerance and breeding rapeseed drought-tolerant varieties.
In this study, Brassica napus var. YAU200908 with good drought-tolerance was used as materials. By analyzing the transcriptomes of roots and leaves under drought stress, we found that there were 9 419 Unigenes and13 230 Unigenes respectively expressed in roots and leaves under drought stress, of which the up-regulated expression was slightly more than the down-regulated expression. The differentially expressed genes(DEGs) have been analyzed in COG database and GO database, and 20 unigenes related to drought-tolerance in YAU200908 were discovered, such as 4 unigenes for transcription factor, 4 unigenes for osmotic adjustment, 4 unigenes for transpiration regulation, 2 unigenes for the removal of reactive oxygen and 6 unigenes for ABA level regulation.The research would provide a basis for illuminating molecular mechanism of rapeseed drought-tolerance and breeding rapeseed drought-tolerant varieties.
引文
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Duan L.J.,Wang P.,Chen M.C.,and Wang S.M.,2015,Cloning outward-rectifying potassium channel PtSKOR gene and constructing its RNAi vector in halophyte Puccinellia tenuiflora,Fenzi Zhiwu Yuzhong(Molecular Plant Breeding),13(4):877-886(段丽婕,王沛,陈梦词,王锁民,2015,盐生植物小花碱茅外整流K+通道PtSKOR基因的克隆及RNAi载体构建,分子植物育种,13(4):877-886)
Kruse E.,Liu Z.L.,and Kloppstech K.,1993,Expression of heat shock proteins during development of barley,Plant Molecular Biology,23(1):111-122
Fatma K.,Dong Y.S.,and Charles L.G.,2006,Roles ofβ-amylase and starch breakdown during temperatures stress,Physiologia Plantarum,126:120-128
Feng J.,Yang Z.J.,Zhou J.P.,Liu C.,Bai L.L.,and Ren Z.L.,2005,Cloning and sequence analysis of zeaxanthin epoxidase gene(ZEP)isolated from Triticum aestivum L.,Xinan Nongye Xuebao(Southwest China Journal of Agricultural Sciences),15(5):603-607(冯娟,杨足君,周建平,刘成,白丽莉,任正隆,2005,小麦玉米黄质环氧化酶(ZEP)cDNA的克隆及序列分析,西南农业学报,15(5):603-607)
Guo Y.F.,and Gan S.S.,2006,At NAP,a NAC family transcription factor has an important role in leaf senescence,Plant Journal for Cell and Molecular Biology,46(4):601-612
Haake V.,Cook D.,Riechmann J.L.,Pineda O.,Thomashow M.F.,and Zhang J.Z.,2002,Ranscription factor CBF4 is a regulat or of drought adaptation in Arabidopsis,Plant Physiology,130(2):639-648
Hashem A.,Majumdar M.N.A.,Hamid A.,and Hossain M.M.,1998,Drought stress effects on seed yield,yield attributes,growth,cell membrane stability and gas exchange of synthesized Brassica napus L.,Journal of Agronomy and Crop Science,180(3):129-136
Hanson A.D.,May A.M.,Grumet R.,Bode J.,Jamieson G.C.,and Rhodes D.,1985,Betaine synthesis in chenopods:Localization in chloroplasts,Proceedings of the National A-cademy of Science of the United States,82(11):3678-3682
He Q.L.,Zhang Z.,Ran C.Y.,and Xie X.Y.,2016,Expression analysis of Brassica napus L.drought-related genes under drought stress,Huadong Shifan Daxue Xuebao(Journal of East China Normal University(Natural Sciences)),2016(1):113-122(何巧丽,张喆,冉春燕,谢小玉,2016,干旱胁迫下甘蓝型油菜相关抗旱基因的表达分析,华东师范大学学报(自然科学版),2016(1):113-122)
Ilami G.,Nespoulous C.,Huet J.C.,Vartanian N.,and Pernollet J.C.,1997,Characterization of BnD22,a drought-induced protein expressed in Brassica napus leaves,Phytochemistry,45(1):1-8
Kasukabe Y.,He L.,Nada K.,Misawa S.,Ihara I.,and Tachibana S.,2004,Overexpression of spermidine synthase enhances tolerance to multiple environmental stresses and up-regulates the expression of various stress-regulated genes in transgenic Arabidopsis thaliana,Plant Cell Physiology,45(6):712-722
Li F.L.,Wu X.M.,Lam P.,Bird D.,Zheng H.Q.,Samuels L.,Jetter R.,and Kunst L.,2008,Identification of the wax ester synthase/acyl-coenzyme A:diacylglycerol acyltransferase WSD1 required for stem wax ester biosynthesis in Arabidopsis,Plant Physiology,148(1):97-107
Li H.,2005,Arabidopsis gene expression pattern analysis andOSR family OSR2 involved in drought stress response of experimental evidence,Dissertation for ph.D.,China agri cultural university,Supervisor:Wu W.H.,pp.74-75(李虹,2005,拟南芥OSR家族基因的表达模式分析及OSR2参与干旱胁迫反应的实验证据,博士学位论文,中国农业大学,导师:武维华,pp.74-75)
Li S.J.,Liu D.,Sun G.L.,Peng S.D.,Chen S.W.,Wang Q.F.,and Lin L.B.,2014,Genetic diversity analysis and molecular identification of Brassic napus varieties mainly planting in Yunnan province,Zhongzi(Seed),33(11):27-31(李淑娟,刘岱,孙桂林,彭少丹,陈升位,王勤方,林良斌,2014,云南主栽甘蓝型油菜品种的遗传多样性分析与分子鉴定,种子,33(11):27-31)
Lu K.,Zhang L.,Qu C.M.,Liang Y.,Tang Z.L.,and Li J.N.,2015,Identification of drought stress-responsive genes in leaves of Brassica napus by RNA sequencing,Zhongguo Nongye Kex ue(Scientia Agricultura Sinica),48(4):630-645(卢坤,张琳,曲存民,梁颖,唐章林,李加纳,2015,利用RNA-Seq鉴定甘蓝型油菜叶片干旱胁迫应答基因,中国农业科学,48(4):630-645)
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