基于HPLC指纹图谱、多成分定量结合化学计量学方法评价不同产地三棱药材的质量
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摘要
目的基于HPLC指纹图谱、多成分定量与化学计量学相结合的方法建立不同产地三棱质量评价方法。方法采用中药色谱指纹图谱相似度评价系统(2004A版)对7省12批不同产地的三棱药材的HPLC指纹图谱进行相似度评价;通过对照品比对指认9种化学成分,并测定样品中9种成分含量;使用SPSS 20.0软件进行聚类分析和主成分分析。结果选取了12个色谱峰作为指纹图谱共有峰,12批样品的相似度计算结果均大于0.800,说明各产地的药材有较好的一致性;测定了5-羟甲基糠醛、香草酸、阿魏酸、对羟基苯甲醛、对羟基苯甲酸、香兰素、原儿茶酸、对香豆酸、异阿魏酸9种成分的含量;通过聚类分析可将12批样品聚为4类;主成分分析用4个主成分对三棱药材进行综合评价,综合得分结果显示,河南新乡、江苏镇江、河北沧州、湖南岳阳、浙江金华、河南郑州的三棱药材在所有样品中的综合得分位于前6名;12批饮片主成分综合得分与含量测定结果存在相关性。结论建立的三棱质量评价方法操作简便、重复性好、结果可靠,可用于全面评价三棱药材的质量。
Objective To establish a combinative method based on HPLC fingerprint, multi-component assay, and pattern recognition for quality evaluation of Sparganii Rhizoma. Methods The similarity analyzed with "Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica 2004 A", Nine kinds of components were identified by comparison with reference substances and their content in samples were determined and hierarchical clustering analysis(HCA) and principal component analysis(PCA) were performed by SPSS 20.0. Results There were 12 common peaks, and the similarity degrees of 12 batches of samples were more than 0.800, which showed that all the samples from different origins were of good consistency. Nine of them were identified as 5-hydroxymethylfurfural, vanillic acid, ferulic acid, p-hydroxybenzaldehyde, p-hydroxybenzoic acid, vanillin, protocatechuic acid, p-coumaric acid, and isoferulic acid; The samples were divided into four clusters by HCA. The result of PCA showed, the quality of samples could be evaluated basically. According to the composite score, the quality of Sparganii Rhizomafrom different origins, Henan Xinxiang, Jiangsu Zhenjiang, Hebei Cangzhou, Hunan Yueyang, Zhejiang Jinhua, and Henan Zhengzhou were better than others. There was a correlation between the scores of the main components of the 12 batches of decoction pieces and the results of the content determination. Conclusion The method is simple, reproducible, and reliable, it can be used for quality control and evaluation of Sparganii Rhizoma from different origins.
Objective To establish a combinative method based on HPLC fingerprint, multi-component assay, and pattern recognition for quality evaluation of Sparganii Rhizoma. Methods The similarity analyzed with "Similarity Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica 2004 A", Nine kinds of components were identified by comparison with reference substances and their content in samples were determined and hierarchical clustering analysis(HCA) and principal component analysis(PCA) were performed by SPSS 20.0. Results There were 12 common peaks, and the similarity degrees of 12 batches of samples were more than 0.800, which showed that all the samples from different origins were of good consistency. Nine of them were identified as 5-hydroxymethylfurfural, vanillic acid, ferulic acid, p-hydroxybenzaldehyde, p-hydroxybenzoic acid, vanillin, protocatechuic acid, p-coumaric acid, and isoferulic acid; The samples were divided into four clusters by HCA. The result of PCA showed, the quality of samples could be evaluated basically. According to the composite score, the quality of Sparganii Rhizomafrom different origins, Henan Xinxiang, Jiangsu Zhenjiang, Hebei Cangzhou, Hunan Yueyang, Zhejiang Jinhua, and Henan Zhengzhou were better than others. There was a correlation between the scores of the main components of the 12 batches of decoction pieces and the results of the content determination. Conclusion The method is simple, reproducible, and reliable, it can be used for quality control and evaluation of Sparganii Rhizoma from different origins.
引文
[1]冯娅茹,张文婷,李二文,等.三棱化学成分及药理作用研究进展[J].中草药,2017,48(22):4804-4818.
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[12]刘昌孝.从中药资源-质量-质量标志物认识中药产业的健康发展[J].中草药,2016,47(18):3149-3154.
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[15]潘卓,邵禾欣,刘婷,等.中药注射剂中安全性相关物质5-羟甲基糠醛的研究进展[J].中国中药杂志,2017,42(10):1842-1846.
[16]Nadal J M,Gomes M L,Borsato D M,et al.Spray-dried solid dispersions containing ferulic acid:Comparative analysis of three carriers,in vitro dissolution,antioxidant potential and in vivo anti-platelet effect[J].Drug Dev IndPharm,2016,42(11):1813-1824.
[17]Krga I,Vidovic N,Milenkovic D,et al.Effects of anthocyanins and their gut metabolites on adenosine diphosphate-induced platelet activation and their aggregation with monocytes and neutrophils[J].Arch Biochem Biophys,2018,645:34-41.
[18]Neog M K,Joshua P S,Krishnan M,et al.p-Coumaric acid,a dietary polyphenol ameliorates inflammation and curtails cartilage and bone erosion in the rheumatoid arthritis rat model[J].Biofactors,2017,43(5):698-717.
[19]Adel A M,Ahmed I Y,Abd El-Twab S M,et al.Gallic acid and p-coumaric acid attenuate type 2diabetes-induced neurodegeneration in rats[J].Metab Brain Dis,2017,32(4):1279-1286.
[20]Jairajpuri D S.Isoferulic acid action against glycation-induced changes in structural and functional attributes of human high-density lipoprotein[J].Biochemistry,2016,81(3):289-295.
[2]张先洪,毛春芹,陆兔林,等.三棱饮片质量标准研究[J].时珍国医国药,2010,21(6):1384-1385.
[3]常乙玲,吴启南,梁侨丽,等.中药三棱HPLC指纹图谱的研究[J].南京中医药大学学报,2010,26(2):135-136.
[4]张海波,高羽,梁侨丽,等.HPLC测定中药三棱中游离阿魏酸和总阿魏酸含量[J].南京中医药大学学报,2011,27(2):169-171.
[5]吴昊,关树光,朴文博,等.中药三棱的化学成分指认和指纹图谱研究[J].中国医药指南,2012,10(34):431-433.
[6]Wang X S,Wu Q N,Wu Y F,et al.Determination of seven phenolic compounds in Rhizoma sparganii by RP-HPLC[J].J Chromatogr Sci,2013,51(4):371-375.
[7]陈广云,吴启南,王新胜,等.生物效价测定法用于活血化瘀中药三棱品质评价的研究[J].中国中药杂志,2012,37(19):2913-1916.
[8]徐男,曹铭晨,黄欣,等.三棱乙酸乙酯部位HPLC指纹图谱及4种酚类成分含量测定[J].中国医院药学杂志,2017,37(18):1783-1786.
[9]袁涛,华会明,裴月湖.三棱的化学成分研究(I)[J].中草药,2005,36(11):1607-1610.
[10]袁涛.中药三棱化学成分的研究[D].沈阳:沈阳药科大学,2006.
[11]王艳.中药三棱的化学成分,质量标准及开发利用研究[D].天津:天津中医学院,2005.
[12]刘昌孝.从中药资源-质量-质量标志物认识中药产业的健康发展[J].中草药,2016,47(18):3149-3154.
[13]刘昌孝,陈士林,肖小河,等.中药质量标志物(Q-Marker):中药产品质量控制的新概念[J].中草药,2016,47(9):1443-1457.
[14]熊亮,彭成.基于中药质量标志物(Q-Marker)的基本条件研究益母草和赶黄草的Q-Marker[J].中草药,2016,47(13):2212-2220.
[15]潘卓,邵禾欣,刘婷,等.中药注射剂中安全性相关物质5-羟甲基糠醛的研究进展[J].中国中药杂志,2017,42(10):1842-1846.
[16]Nadal J M,Gomes M L,Borsato D M,et al.Spray-dried solid dispersions containing ferulic acid:Comparative analysis of three carriers,in vitro dissolution,antioxidant potential and in vivo anti-platelet effect[J].Drug Dev IndPharm,2016,42(11):1813-1824.
[17]Krga I,Vidovic N,Milenkovic D,et al.Effects of anthocyanins and their gut metabolites on adenosine diphosphate-induced platelet activation and their aggregation with monocytes and neutrophils[J].Arch Biochem Biophys,2018,645:34-41.
[18]Neog M K,Joshua P S,Krishnan M,et al.p-Coumaric acid,a dietary polyphenol ameliorates inflammation and curtails cartilage and bone erosion in the rheumatoid arthritis rat model[J].Biofactors,2017,43(5):698-717.
[19]Adel A M,Ahmed I Y,Abd El-Twab S M,et al.Gallic acid and p-coumaric acid attenuate type 2diabetes-induced neurodegeneration in rats[J].Metab Brain Dis,2017,32(4):1279-1286.
[20]Jairajpuri D S.Isoferulic acid action against glycation-induced changes in structural and functional attributes of human high-density lipoprotein[J].Biochemistry,2016,81(3):289-295.