摘要
大丽轮枝菌是一种重要的土传植物病原真菌,以休眠结构微菌核作为初始接种体,可侵染660多种植物引致黄萎病。微菌核是致密的多细胞结构,表面附着大量的DHN黑色素。许多报道指出,在微菌核发育过程中,传统的DHN黑色素合成途径中有5种催化酶编码基因Vd PKS、Vd T4HR、Vd SCD、Vd T3HR和Vd LAC均被诱导表达,但这些基因与微菌核形成的关系目前尚无报道。本研究通过基因敲除技术,系统研究了传统DHN黑色素合成通路上这5种关键酶编码基因及一种缩链催化酶编码基因Vayg1在大丽轮枝菌黑色素合成及微菌核形成中的作用。结果表明,大丽轮枝菌DHN黑色素合成需要Vayg1基因的参与,且Vayg1和Vd T3HR基因还参与微菌核的形成过程。因此,Vayg1基因和Vd T3HR基因可作为黄萎病防治的新靶标。
The soil-born plant fungal pathogen Verticillium dahliae, with its resting structure of microsclerotia as primary inoculum, can cause Verticillium wilt on more than 660 plant species. The well-developed microsclerotia are thick-walled structures, where DHN melanin particles are deposited. Many studies suggest that expressions of five genes, Vd PKS, VdT4HR, Vd SCD, VdT3HR and Vd LAC, encoding five enzymes involved in DHN melanin biosynthesis were induced during microsclerotium development. However, their role in microsclerotium formation remains unclear. In the current study, melanin deficient mutants generated by deletion of these five genes and the Vayg1 gene, which is responsible for chain-length shortening of a precursor,were used to systemically elucidate their role on melanin production and microsclerotium formation in V. dahliae. The results showed that Vayg1 is required for DHN melanin production in V. dahliae. Vayg1 and Vd T3 HR are involved in microsclerotium formation, indicating that these two enzymes would be potential targets for Verticillium wilt management.
引文
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