摘要
实验以虹鳟为研究对象,选取17种中草药开展中草药免疫增强剂的体外快速筛选研究。采用Percoll密度梯度离心方法,对虹鳟外周血白细胞进行分离纯化实验。结果表明:白头翁、桂桔、白芨、大黄、贯众、红景天6种中草药的SPO显著高于对照组(P<0.05),且NBTN显著提高[NBTB(%)>15%];白芨、厚朴、麻黄、丹参、大黄、马齿苋、泽泻、黄柏、苍耳子、贯众、红景天组的吞噬活性(PA)值显著高于对照组(P<0.05),蒲公英组的吞噬指数(PI)值显著高于对照组(P<0.05)。综合上述评价指标:白芨、大黄、贯众、红景天可作为虹鳟免疫增强剂的备选中草药。
This research was conducted to deter-mine 17 Chinese herbal medicines were selectedfor rapid screening of immunostimulants of O.mykiss in vitro. Percoll density gradient centrifu-gation methods was used to isolate and purifywhite blood cells from peripheral blood of O.mykiss. The results showed that SPO-values ofAnemone chinensis, Cassia twig, Rhizoma bletil-lae, Radixet rhizomarhei, Cyrtomium rhizomeand Rhodiola rosea were significantly highest compared with control(P<0.05), and NBTBvalues were significantly improved[NBTB(%)>15%]. That PAvalues of Rhizoma bletillae, Cyrtomium rhizome, Rhodiola rosea, Cortex phellodendri, Fructus xanthii,Radix et rhizoma rhei, Port ulaca oleracea L, Rhizoma alismatis, Herba ephedrae, Radix salviae miltior-rhizae and Magnolia bark were significantly highest compared with control group(P<0.05), the PI val-ues of Taraxacum mongolicum were significantly highest compared with control group(P<0.05). Over-all, four kinds of Chinese herbal medicines such as white Radix Paeoniae Alba, Radix et rhizoma rhei,Cyrtomium rhizome, and Rhodiola rosea can be used as immunostimulants for O. mykiss.
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