耳复康口服液对小鼠微循环障碍的影响
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摘要
目的:观察耳复康口服液对肾上腺素致急性血瘀症小鼠微循环障碍的影响。方法:将小鼠随机分为模型组,脑得生片组(1.35 g·kg~(-1)),高(30 ml·kg~(-1))、中(15 ml·kg~(-1))、低(7.5 ml·kg~(-1))剂量耳复康口服液组,用微循环仪观察正常小鼠给药1 h后毛细血管开放数。尾静脉注射肾上腺素造成耳廓微循环障碍,观察造模后2 min小鼠的毛细血管开放数及血流情况。结果:和模型组相比,高、中、低剂量耳复康口服液对正常小鼠耳廓毛细血管开放数无明显影响(P>0.05);与模型组相比,高、中剂量耳复康口服液可显著改善肾上腺素致小鼠耳壳微循环障碍模型微循环血流情况(P<0.05或P<0.01),可明显对抗肾上腺素所致小鼠耳壳微循环障碍模型毛细血管网开放数的减少(P<0.05)。结论:耳复康口服液有改善微循环障碍的作用。
Objective: To observe the effect of Erfukang oral liquid on microcirculation disturbance in mice with acute blood stasis caused by adrenaline. Methods: The mice were randomly divided into the blank group,Naodesheng group( 1. 35 g·kg~(-1)),Erfukang oral liquid group respectively at high( 30 ml·kg~(-1)),medium( 15 ml·kg~(-1)) and low( 7. 5 ml·kg~(-1)) dose,and a microcirculation apparatus was used to observe the number of capillary opening in the normal mice at one hour after the administration. The microcirculation disturbance of auricle was induced by the injection of epinephrine into caudal vein. The number of capillary opening and blood flow in 2 min in the mice were observed. Results: Compared with the blank group,Ergukang oral liquid at high,medium and low dose showed effect on the number of opening capillaries without statistical significance( P > 0. 05); compared with the model group,Erfukang oral liquid at high and medium dose could significantly improve the microcirculation induced by epinephrine( P < 0. 05 or P <0. 01),and significantly confront the decreased number of capillary network opening induced by adrenaline( P < 0. 05). Conclusion:Erfukang oral liquid can improve microcirculation disturbance.
Objective: To observe the effect of Erfukang oral liquid on microcirculation disturbance in mice with acute blood stasis caused by adrenaline. Methods: The mice were randomly divided into the blank group,Naodesheng group( 1. 35 g·kg~(-1)),Erfukang oral liquid group respectively at high( 30 ml·kg~(-1)),medium( 15 ml·kg~(-1)) and low( 7. 5 ml·kg~(-1)) dose,and a microcirculation apparatus was used to observe the number of capillary opening in the normal mice at one hour after the administration. The microcirculation disturbance of auricle was induced by the injection of epinephrine into caudal vein. The number of capillary opening and blood flow in 2 min in the mice were observed. Results: Compared with the blank group,Ergukang oral liquid at high,medium and low dose showed effect on the number of opening capillaries without statistical significance( P > 0. 05); compared with the model group,Erfukang oral liquid at high and medium dose could significantly improve the microcirculation induced by epinephrine( P < 0. 05 or P <0. 01),and significantly confront the decreased number of capillary network opening induced by adrenaline( P < 0. 05). Conclusion:Erfukang oral liquid can improve microcirculation disturbance.
引文
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8 张冬慧,廖明琪,徐培涵,等.去甲肾上腺素和异丙肾上腺素对猪冠状动脉的舒张作用及机制研究[J].中国药师,2012,15(8):1073-1076
9 张榕文,李耿,刘丹丹,等.中药育发液对肾上腺素致小鼠耳廓微循环障碍的影响研究[J].中国当代医药学,2011,18(30):7-8
10 王希东,郝少君,李文俊.抗眩颗粒对小鼠脑膜微循环的影响[J].实用药物与临床,2015,18(6):653-655
2 唐丽燕,李利民,黄利,等.川芎提取物对大鼠主动脉环及小鼠微循环障碍的影响研究[J].中药药理与临床,2013,29(1):105-107
3 赵京生,易伟国.心得宁口服液对微循环障碍模型鼠动静脉管径及毛细血管开放数的影响[J].实用医药杂志,2013,30(9):820-8214路放,杨世海,孟宪兰.人参药理作用研究新进展[J].人参研究,2013,25(1):46-52
5 乔逸,杨志福,奚苗苗,等.羟基红花黄色素A在血瘀和正常大鼠体内药动学研究[J].中国药师,2009,12(8):995-997
6 马丁,王芍,孔德宁,等.白藜芦醇二聚体δ-viniferin及其乙酰化物对小鼠耳廓微循环的影响[J].中国药学杂志,2011,46(5):348-352
7 曹南开,林苗,朱裕勋,等.微循环技术及其在中药研究中的应用进展[J].微循环学杂志,2015,25(3):72-75
8 张冬慧,廖明琪,徐培涵,等.去甲肾上腺素和异丙肾上腺素对猪冠状动脉的舒张作用及机制研究[J].中国药师,2012,15(8):1073-1076
9 张榕文,李耿,刘丹丹,等.中药育发液对肾上腺素致小鼠耳廓微循环障碍的影响研究[J].中国当代医药学,2011,18(30):7-8
10 王希东,郝少君,李文俊.抗眩颗粒对小鼠脑膜微循环的影响[J].实用药物与临床,2015,18(6):653-655