芍药汤对湿热内蕴型溃疡性结肠炎大鼠疾病活动指数及血清IL-8的影响
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摘要
目的:探讨芍药汤对湿热内蕴型溃疡性结肠炎(UC)大鼠疾病活动指数及血清中白介素8(IL-8的影响。方法:将60只健康Wistar大鼠按照随机数字表法分为正常对照组、模型对照组、芍药汤高、中、低剂量组、柳氮磺吡啶(SASP)组。湿热内蕴型溃疡性结肠炎模型大鼠应用高脂高糖辛辣饮食加免疫复合法+TNBS(2,4,6-三硝基苯磺酸)结合乙醇方法复制。按照IL-8 ELISA试剂盒操作说明测定血清中的IL-8水平。在实验开始后24小时、造模成功后24小时、给药治疗后20天对各组大鼠进行疾病活动指数(DAI)评价。结果:DAI:在造模成功后24小时,空白对照组与其余各组相比较疾病活动指数差异有统计学意义(P<0.05)。在治疗后第20天时,空白对照组与各组相比差异有统计学意义(P<0.05),模型对照组与不同剂量的芍药汤组和SASP组对比具差异有统计学意义(P<0.05)。各组大鼠血清中IL-8含量:与正常对照组相比,模型对照组大鼠血清中IL-8含量明显升高,具有统计学意义(P<0.05)。与模型对照组相比较,各剂量芍药汤组、SASP组大鼠血清IL-8含量明显降低,组间比较差异有统计学意义(P<0.05)。结论:芍药汤可降低IL-8的含量,降低组织中炎性细胞含量,并加快对肠黏膜修复的作用。
Objective: To explore the effects of ShaoYao decoction on disease activity indexes and the levels of IL-8 of the rats with ulcerative colitis(UA) of damp-heat internal accumulation pattern. Methods: Sixty rats were divided into normal control group, model control group, high, moderate and low dose groups of ShaoYao decoction,SASP group. rat models suffering UC of damp-heat internal accumulation pattern were replicated by applying high-fat high-sugar acrid diet and immune complex method+TNBS combined with alcohol method. The levels of IL-8 were detected on the basis of the operation instruction of IL-8 ELISA kit. DAI of the rats in different groups were assessed over 24 hours after the experiment began, 24 hours after successfully establishing the models and 20 days after treating. Results: DAI: Over 24 hours after successfully establishing the models, statistical difference was found in DAI among blank control group and others groups(P < 0.05). Over 20 days after treating, the differences were statistically significant when blank control group was compared with the others(P < 0.05). Statistical differences were found when model control group was compared with different dose groups of ShaoYao decoction and SASP group(P< 0.05). The contents of IL-8 in the rats of different groups: Compared with normal control group,the contents of IL-8 raised notably in the rats of model control group, and the difference had statistical meaning(P < 0.05).Compared with model control group, the contents of IL-8 lowered obviously in different dose groups of ShaoYao decoction and SASP group, the differences had statistical meaning(P< 0.05). Conclusion: ShaoYao decoction could decrease the contents of IL-8, reduce the contents of inflammatory cells in the tissue and accelerate the recovery of intestinal mucosa.
Objective: To explore the effects of ShaoYao decoction on disease activity indexes and the levels of IL-8 of the rats with ulcerative colitis(UA) of damp-heat internal accumulation pattern. Methods: Sixty rats were divided into normal control group, model control group, high, moderate and low dose groups of ShaoYao decoction,SASP group. rat models suffering UC of damp-heat internal accumulation pattern were replicated by applying high-fat high-sugar acrid diet and immune complex method+TNBS combined with alcohol method. The levels of IL-8 were detected on the basis of the operation instruction of IL-8 ELISA kit. DAI of the rats in different groups were assessed over 24 hours after the experiment began, 24 hours after successfully establishing the models and 20 days after treating. Results: DAI: Over 24 hours after successfully establishing the models, statistical difference was found in DAI among blank control group and others groups(P < 0.05). Over 20 days after treating, the differences were statistically significant when blank control group was compared with the others(P < 0.05). Statistical differences were found when model control group was compared with different dose groups of ShaoYao decoction and SASP group(P< 0.05). The contents of IL-8 in the rats of different groups: Compared with normal control group,the contents of IL-8 raised notably in the rats of model control group, and the difference had statistical meaning(P < 0.05).Compared with model control group, the contents of IL-8 lowered obviously in different dose groups of ShaoYao decoction and SASP group, the differences had statistical meaning(P< 0.05). Conclusion: ShaoYao decoction could decrease the contents of IL-8, reduce the contents of inflammatory cells in the tissue and accelerate the recovery of intestinal mucosa.
引文
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[8]Guimbaud R,Bertrand V,Chauvelot-Moachon L,et al.Network of inflammatory cytokines and correlation with disease activity in ulcerative colitis[J].American Journal of Gastroenterology,1998,93(12):2397-2404.
[9]王允娜,牛崇信.温通针法治疗脾肾阳虚型溃疡性结肠炎临床疗效及对相关炎性因子的影响[J].西部中医药,2017,30(5):114-116.
[10]王福亭.致康胶囊对溃疡性结肠炎患者血清TNF-α、IL-6及CRP的影响[J].西部中医药,2016,29(2):100-102.
[11]郭军雄,马丽,王小莲.风药对兔结肠黏膜蛋白与乙酸诱发大鼠溃疡性结肠炎的治疗作用[J].西部中医药,2012,25(3):21-23.
[12]李明,韩文冬,黄雅慧.中西医结合治疗溃疡性结肠炎临床疗效及其对血小板计数的影响[J].西部中医药,2011,24(7):79-81.
[2]易蕊,常娟,刘晓艳,等.TNF-α、IL-6及IL-8在不同程度溃疡性结肠炎患者血清中的表达及意义[J].现代生物医学进展,2015,15(14):2641-2644.
[3]赵党生,晋梅,李琳,冯,等.参白合剂对溃疡性结肠炎模型小鼠疗效及对IL-10和TNF-α表达的影响[J].中医研究,2012,25(3):64-66.
[4]Uguccioni M,Gionchetti P,Robbiani DF,et al.Increased expression of IP-10,IL-8,MCP-1 and MCP-3 in ulcerative colitis[J].American Journal of Pathology,1999,155(2):331-336.
[5]刘凯,熊晶晶,刘梅,等.酪酸梭菌、美沙拉嗪、蒙脱石散对溃疡性结肠炎大鼠血IL-6、IL-8、IL-10及TNF-α水平的影响[J].免疫学杂志,2015,31(11):931-936
[6]张军,叶玉伟.中医药治疗溃疡性结肠炎研究进展概况[J].西部中医药,2013,26(12):150-152.
[7]景姗,王新月,杨雪,等.溃疡性结肠炎大鼠肺、肠组织中TGF-β1、SP-A表达的改变及中药的干预作用[J].西部中医药,2014,27(3):10-15.
[8]Guimbaud R,Bertrand V,Chauvelot-Moachon L,et al.Network of inflammatory cytokines and correlation with disease activity in ulcerative colitis[J].American Journal of Gastroenterology,1998,93(12):2397-2404.
[9]王允娜,牛崇信.温通针法治疗脾肾阳虚型溃疡性结肠炎临床疗效及对相关炎性因子的影响[J].西部中医药,2017,30(5):114-116.
[10]王福亭.致康胶囊对溃疡性结肠炎患者血清TNF-α、IL-6及CRP的影响[J].西部中医药,2016,29(2):100-102.
[11]郭军雄,马丽,王小莲.风药对兔结肠黏膜蛋白与乙酸诱发大鼠溃疡性结肠炎的治疗作用[J].西部中医药,2012,25(3):21-23.
[12]李明,韩文冬,黄雅慧.中西医结合治疗溃疡性结肠炎临床疗效及其对血小板计数的影响[J].西部中医药,2011,24(7):79-81.