一株甲醛降解菌鉴定及其降解性能研究
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摘要
目的:从实验室的动物肝脏浸置标本分离得到一株甲醛降解菌CSLG1,并考察其对甲醛的降解特性。方法:通过形态学和18S rDNA测序鉴定菌种,改变液体培养基中甲醛浓度确定该菌株的甲醛抗性,并在较高甲醛浓度的培养基中测定菌株CSLG1的生长曲线,证实其对甲醛具有降解能力。结果:鉴定结果显示,该菌CSLG1属于拟青霉属(Paecilomyces daclylethromorphus),其能在6.52 g/L的高甲醛浓度下生长;当甲醛初始浓度为3.73 g/L时,菌株CSLG1能在60 h内彻底降解。结论:菌种CSLG1对甲醛具有良好的降解效率,研究结果对甲醛污染环境的生物降解具有较好的科学意义和应用价值。
Objective: To isolate the strain,which was capable of degrading formaldehyde from animal liver immersed specimens collected from laboratory,and investigate its degradation characteristics. Method: The strain was identified based on standard morphological identification and 18S rDNA sequence analysis. The capability of resistance to formaldehyde was determined by changing formaldehyde concentration in culture. The growth curve of strain CSLG1 was studied in culture containing higher formaldehyde concentration,which was based on making research on its ability of degrading formaldehyde. Result: The strain CSLG1 was identified as Paecilomyces daclylethromorphus. Its anti- formaldehyde capacity was 6. 52 g / L,which was much higher than other microorganism reported. When formaldehyde initial concentration was 3. 73 g / L,it could be degraded completely by strain CSLG1 after 60 h. Conclusion: Strain CSLG1 was effective in degrading formaldehyde,the results were of significance and application importance in the study of formaldehyde biodegradation.
Objective: To isolate the strain,which was capable of degrading formaldehyde from animal liver immersed specimens collected from laboratory,and investigate its degradation characteristics. Method: The strain was identified based on standard morphological identification and 18S rDNA sequence analysis. The capability of resistance to formaldehyde was determined by changing formaldehyde concentration in culture. The growth curve of strain CSLG1 was studied in culture containing higher formaldehyde concentration,which was based on making research on its ability of degrading formaldehyde. Result: The strain CSLG1 was identified as Paecilomyces daclylethromorphus. Its anti- formaldehyde capacity was 6. 52 g / L,which was much higher than other microorganism reported. When formaldehyde initial concentration was 3. 73 g / L,it could be degraded completely by strain CSLG1 after 60 h. Conclusion: Strain CSLG1 was effective in degrading formaldehyde,the results were of significance and application importance in the study of formaldehyde biodegradation.
引文
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[2]黄赛花,陈能场,毕鸿亮.3株霉菌对甲醛转化能力的研究[J].中国环境科学,2008,28(12):111-115.
[3]梁建聪,郭云霞,容学军.甲醛污染来源及防治措施[J].广西轻工业,2009,25(3):100-101.
[4]牛凤兰,宋德锋,陈霖,等.环境中甲醛污染来源及检测方法新进展[J].上海预防医学,2009,21(9):453-455.
[5]李宁慧,黄翔和,谢乐飞.一株甲醛降解菌的分离筛选与降解特性研究[J].高校理科研究,2008,28:86-87.
[6]谢文娟,王洁,孙珮石,等.高效降解甲醛菌株的分离鉴定及其特性[J].微生物学通报,2011,38(11):1626-1631.
[7]Mingbo Qu,Sanjoy K.Bhattacharya.Toxicity and biodegradation of formaldehyde in anaerobic methanogenic culture[J].Biotechnology and Bioengineering,1997,55(95):727-736.
[8]徐云,金晶,郑重,等.高活性高耐受甲醛降解菌株的分离鉴定及降解条件研究[J].环境科学,2010,31(10):2481-2486.
[9]国家环境保护总局.GB13197-1991水质甲醛的测定——乙酰丙酮分光光度法[S].北京:国家质量监督检验检疫总局,2005.
[10]宋鸽.抗甲醛微生物分分离鉴定及其相关基因的克隆[D].哈尔滨师范大学硕士学位论文,2011:45.
[11]Thomas J.White,Steven B.Lee,Thomas D.Bruns,et al.Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics[C]//INNIS M A,GELFAND D H,SNINSKY J J,et al.PCR Protocols:A Guide to Methods and Applications.San Diego:Academic Press,1990:315-322.
[12]曹培义.邻甲苯胺分光光度法测定甜饮料中的葡萄糖[J].职业与健康,2001,8(11):45-46.
[13]黄赛花,陈能场.一株甲醛降解真菌Aspergillus spp.H4的分离鉴定[J].生态环境,2007,16(4):1175-1179.
[14]李章良,林小园,林勇,等.甲醛降解菌的筛选及降解特性研究[J].环境工程学报,2011,5(11):2547-2551.
[15]Saeed Mirdamadi,Afsaneh Rajabi,Pooneh Khalilzadeh,et al.Isolation of bacteria able to metabolize high concentration of formaldehyde[J].World Journal of Microbiology and Biotechnology,2005,10(21):1299-1301.
[16]Tomohioko Yamazaki,Wakako Tsugawa,Koji Sode.Biodegradation of formaldehyde by a formaldehyde-resistant bacterium isolated from seawater[J].Applied Biochemistry and Biotechnology,2001,2(91-93):213
[17]Ryoji Mitsui,Masahiro Omori,Hideski Kitazawa,et al.Formaldehyde-limited cultivation of a newly isolated methylotrophic bacterium,Methylobacterium sp.MF1:enzymatic analysis related to C1 metabolism[J].Journal of Bioscience and Bioengineering,2005,99(1):18-22.