重金属铅对绢毛委陵菜光合特性及荧光参数的影响
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摘要
为探讨不同浓度重金属Pb~(2+)胁迫对绢毛委陵菜(Potentilla sericea L.)光合特性及荧光参数的影响,本文采用盆栽的方法,通过对绢毛委陵菜净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)、胞间CO_2浓度(Ci)等多项指标的测定,并对其变化规律做了比较研究。结果表明:绢毛委陵菜胞间CO_2浓度(Ci)随Pb~(2+)胁迫时间的延长及胁迫浓度的增大呈上升趋势,而净光合速率(Pn)、蒸腾速率(Tr)、气孔导度(Gs)变化趋势与胞间CO_2浓度(Ci)变化趋势相反;Pb~(2+)胁迫50d时PSII潜在活性(Fv/Fo)、最大光化学效率(Fv/Fm)均随胁迫浓度的增大逐渐降低;绢毛委陵菜在Pb~(2+)浓度为300mg·kg-1、600mg·kg~(-1)时能够生存,但生长缓慢。研究表明随着铅浓度的提高,铅通过影响植物的非气孔限制来影响绢毛委陵菜的光合特性。
The net photosynthetic rate(Pn)and transpiration rate of Potentilla sericea Linn.(Tr),stomatal conductance(Gs),intercellular CO_2 concentration(Ci)and other indicators were determined to investigate the effects of different concentrations of Pb~(2+) on the photosynthetic characteristics and fluorescence parameters of Potentilla sericea Linn.A pot experiment was conducted in greenhouse to investigate their change patterns.Our results showed that the intercellular CO_2 concentration(Ci)increased with the prolongation of Pb~(2+) stress and the increase of stress.However the net photosynthetic rate(Pn),transpiration rate(Tr),and stomatal conductance(Gs)were opposite to change of intercellular CO_2 concentration(Ci).The potential activity(Fv/Fo)and the maximum photochemical efficiency(Fv/Fm)of PSII gradually decreased with increasing Pb~(2+) stress concentration at 50 d.Potentilla sericea Linn.was capable of surviving at concentrations of 300 mg·kg~(-1) and 600 mg·kg-1 Pb~(2+) ,but the growth was slow.These results showed that it affected the photosynthetic characteristics of Potentilla sericea Linn.by affecting the nonstomatal limitation of the plant.
The net photosynthetic rate(Pn)and transpiration rate of Potentilla sericea Linn.(Tr),stomatal conductance(Gs),intercellular CO_2 concentration(Ci)and other indicators were determined to investigate the effects of different concentrations of Pb~(2+) on the photosynthetic characteristics and fluorescence parameters of Potentilla sericea Linn.A pot experiment was conducted in greenhouse to investigate their change patterns.Our results showed that the intercellular CO_2 concentration(Ci)increased with the prolongation of Pb~(2+) stress and the increase of stress.However the net photosynthetic rate(Pn),transpiration rate(Tr),and stomatal conductance(Gs)were opposite to change of intercellular CO_2 concentration(Ci).The potential activity(Fv/Fo)and the maximum photochemical efficiency(Fv/Fm)of PSII gradually decreased with increasing Pb~(2+) stress concentration at 50 d.Potentilla sericea Linn.was capable of surviving at concentrations of 300 mg·kg~(-1) and 600 mg·kg-1 Pb~(2+) ,but the growth was slow.These results showed that it affected the photosynthetic characteristics of Potentilla sericea Linn.by affecting the nonstomatal limitation of the plant.
引文
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[19]杨文权,褚继鹏,寇建村,等.遮阴对白三叶叶片解剖结构和光合特性的影响[J].草地学报,2015,23(3):653-656
[20]Krupa Z,Baranowka M,Orzol D.Can anthocya 2nins be considered as heavy metal Indicator in higher Plant[J].Acta Physiologiae Plantarum,1996,18:147-151
[21]刘洋,胡芳洁,陈良华,等.3种土壤类型下红椿幼苗对铅胁迫的光合响应[J].西北植物学报,2014,34(7):1411-1418
[22]李文娆,张岁岐,山仑.水分胁迫对紫花苜蓿根系吸水与光合特性的影响[J].草地学报,2007,15(3):206-211
[23]潘宝贵,王述彬,刘金兵,等.高温胁迫对不同辣椒品种苗期光合作用的影响[J].江苏农业学报,2006,22(2):137-140
[24]Farquhar G D,Sharkey T D.Stomatal conductance and photosynthesis[J].Annual Review of Plant Physiology,1982,33(1):317-345
[25]Ralph P J,Gademann R.Rapid light curves:A powerful tool to assess photosynthetic activity[J].Aquatic Botany,2005,82(3):222-237
[26]汪明霞,孙国荣,王建波,等.NaCl胁迫下星星草幼苗MDA含量与膜透性及叶绿素荧光参数之间的关系[J].生态学报,2006,26(1):122-129
[27]Maxwellk,Johnsongn.Chlorophyll fluorescence-apractical guide[J].Journal of Experimental Botany,2000,51(345):659-668
[28]Sarijevag,Knappm,Lichtenthalerhk.Differences in photosynthetic activity,chlorophyll and carotenoid levels,and in chlorophyll fluorescence parameters ingreen sun and shade lea-ves of Ginkgo and Fagus[J].Journal of Plant Physiology,2007,164(7):950-955
[29]韩张雄,李利,徐新文,等.NaCl胁迫对3种荒漠植物幼苗叶绿素荧光参数的影响[J].西北植物学报,2008,28(9):1843-1849
[30]万雪琴,张帆,夏新莉,等.镉处理对杨树光合作用及叶绿素荧光参数的影响[J].林业科学,2008,44(6):73-78
[31]刘晓静,张晓磊,齐敏兴,等.混合盐碱对紫花苜蓿种子萌发及幼苗期叶绿素荧光特性的影响[J].草地学报,2013,21(3):501-507
[2]刘大林,曹喜春,张华,等.铅、镉胁迫对饲用高粱部分生理指标的影响[J].草地学报,2014,22(1):122-125
[3]周芙蓉,王进鑫,杨楠,等.水分和铅胁迫对土壤酶活性的影响[J].草地学报,2013,21(3):479-484
[4]唐咏.铅污染对辣椒幼苗生长及SOD和POD活性的影响[J].沈阳农业大学学报,2001,32(1):26-28.
[5]苗明升,朱圆圆,曹明霞,等.重金属铅对玉米种子萌发和早期生长发育的影响[J].山东师范大学学报:自然科学版,2003,18(1):82-84
[6]Sharma P,Dubey R S.Lead toxicity in plants[J].Brazilian Journal of Plant Physiology,2005,17(1):35-52
[7]王慧忠.匍匐剪股颖和多年生黑麦草对镉、铅胁迫的响应及部分基因表达调控机理研究[D].雅安:四川农业大学,2006:5-6
[8]罗红艳.3种木本植物幼树重金属抗性的比较研究[D].南京:南京林业大学,2003:7-8
[9]孙航,曲波,张延菊.饲草植物委陵菜属(Potentma L)的化学成分[J].饲料工业及其资源植物的研究进展,2010,31(2):58-60
[10]吴建慧,郭瑶,崔艳桃.自然失水胁迫对绢毛委陵菜叶片超微结构的影响[J].东北林业大学学报,2011,39(11):53-55
[11]黄晓华,周青,程宏英.五种常绿树木对铅污染胁迫的反应[J].城市环境与城市生态,2000,13(6):48-50
[12]Ralph P J,Gademann R.Rapid light curves:A powerful tool to assess photosynthetic activity[J].Aquatic Botany,2005,82(3):222-237
[13]陈建明,俞晓平,程家安.叶绿素荧光动力学及其在植物抗逆生理研究中的应用[J].浙江农业学报,2006,18(1):51-55
[14]彭鸣,王焕校,吴玉树.镉铅诱导玉米苗细胞的超微结构的变化[J].中国环境科学,1991,11(6):426-431
[15]Klaus Q,Buzzer J.Chlorophyll content,cells and chloroplast number and cadmium distribution in Cd-treated cucumber plants[J].Acta Physiologies Plant arum,1985,7(3):139-147
[16]曹玲,王庆成,崔东海.土壤镉污染对四种阔叶树苗木叶绿素荧光特性和生长的影响[J].应用生态学报,2006,17(5):769-772
[17]刘飞虎.植物品种净光合速率测定的取样技术-以苎麻(Boehemeria nivea)为例[J].云南大学学报(自然科学版),2010,32(2):221-226
[18]牟兰,何承刚,姜华,等.干热胁迫对紫花苜蓿光合特性的影响[J].草地学报,2014,22(3):550-555
[19]杨文权,褚继鹏,寇建村,等.遮阴对白三叶叶片解剖结构和光合特性的影响[J].草地学报,2015,23(3):653-656
[20]Krupa Z,Baranowka M,Orzol D.Can anthocya 2nins be considered as heavy metal Indicator in higher Plant[J].Acta Physiologiae Plantarum,1996,18:147-151
[21]刘洋,胡芳洁,陈良华,等.3种土壤类型下红椿幼苗对铅胁迫的光合响应[J].西北植物学报,2014,34(7):1411-1418
[22]李文娆,张岁岐,山仑.水分胁迫对紫花苜蓿根系吸水与光合特性的影响[J].草地学报,2007,15(3):206-211
[23]潘宝贵,王述彬,刘金兵,等.高温胁迫对不同辣椒品种苗期光合作用的影响[J].江苏农业学报,2006,22(2):137-140
[24]Farquhar G D,Sharkey T D.Stomatal conductance and photosynthesis[J].Annual Review of Plant Physiology,1982,33(1):317-345
[25]Ralph P J,Gademann R.Rapid light curves:A powerful tool to assess photosynthetic activity[J].Aquatic Botany,2005,82(3):222-237
[26]汪明霞,孙国荣,王建波,等.NaCl胁迫下星星草幼苗MDA含量与膜透性及叶绿素荧光参数之间的关系[J].生态学报,2006,26(1):122-129
[27]Maxwellk,Johnsongn.Chlorophyll fluorescence-apractical guide[J].Journal of Experimental Botany,2000,51(345):659-668
[28]Sarijevag,Knappm,Lichtenthalerhk.Differences in photosynthetic activity,chlorophyll and carotenoid levels,and in chlorophyll fluorescence parameters ingreen sun and shade lea-ves of Ginkgo and Fagus[J].Journal of Plant Physiology,2007,164(7):950-955
[29]韩张雄,李利,徐新文,等.NaCl胁迫对3种荒漠植物幼苗叶绿素荧光参数的影响[J].西北植物学报,2008,28(9):1843-1849
[30]万雪琴,张帆,夏新莉,等.镉处理对杨树光合作用及叶绿素荧光参数的影响[J].林业科学,2008,44(6):73-78
[31]刘晓静,张晓磊,齐敏兴,等.混合盐碱对紫花苜蓿种子萌发及幼苗期叶绿素荧光特性的影响[J].草地学报,2013,21(3):501-507