辐射诱变及其在大豆育种中的应用
详细信息    查看全文 | 推荐本文 |
  • 英文篇名:Irradiation-induced mutation and its application in soybean breeding
  • 作者:王雪 ; 刘长锴 ; 涂冰洁 ; 张秋英
  • 英文作者:WANG Xue;LIU Changkai;TU Bingjie;ZHANG Qiuying;Key Laboratory of Soybean Molecular Design and Breeding,Northeast Institute of Geography and Agroecology,CAS;University of Chinese Academy of Sciences;
  • 关键词:大豆 ; 辐射 ; 诱变 ; 育种
  • 英文关键词:soybean;;irradiation;;mutation;;breeding
  • 中文刊名:TRZW
  • 英文刊名:Soils and Crops
  • 机构:中国科学院东北地理与农业生态研究所大豆分子设计育种重点实验室;中国科学院大学;
  • 出版日期:2018-09-15
  • 出版单位:土壤与作物
  • 年:2018
  • 期:v.7;No.27
  • 基金:主要农作物诱变育种项目(2016YFD0102105)
  • 语种:中文;
  • 页:TRZW201803004
  • 页数:10
  • CN:03
  • ISSN:23-1580/S
  • 分类号:29-38
摘要
辐射诱变是创制大豆种质资源和选育新品种的有效途径。本文简要阐述了辐射诱变育种的基本原理和常用的诱变源,从表型水平、生理水平和分子水平3个方面总结了大豆辐射诱变的主要生物学效应,概述了国内外大豆诱变育种中诱变后代的筛选方法和主要突变类型的研究进展,讨论分析了大豆诱变育种工作中的难点,提出了辐射育种及其技术今后需要加强的几个方面,以期明确辐射诱变的作用机理,推进辐射诱变在大豆品种选育中的广泛应用。
        Mutation induced by irradiation is an effective method for both creating germplasm resources and developing new varieties in soybean. In this paper,we briefly elaborated basic principle of irradiation-induced mutation breeding and the commonly used mutagenic agents. We summarized the main biological effects of soybean radiation mutation breeding at phenotypic level,physiological level and molecular level. Technical methods in identifying and screening target mutants in mutated offsprings and research progress of main type mutants in soybean by irradiation-induced mutation were overviewed. Problems to be solved in soybean mutation breeding were also discussed. We highlighted several issues that require further investigation with aims in understanding the mechanisms of irradiation-induced mutation and promoting its extensive application in soybean breeding.
引文
[1]赵团结,盖钧镒.栽培大豆起源与演化研究进展[J].中国农业科学,2004(07):954-962.ZHAO T J,GAI J Y. The origin and evolution of cultivated soybean[Glycine max(L.)Merr.][J]. Scientia Agricultura Sinica,2004(7):954-962.
    [2] SINGH R J,HYMOWITZ T. Soybean genetic resources and crop improvement[J]. Génome,1999,42(4):605-616.
    [3] FAO. Food and Agriculture Organization[DB/OL].[2018-04-09]. http://www. fao. org/faostat/en/#data/QC.
    [4]张瑞军,师颖,穆志新,等.我国大豆育种的现状与发展对策[J].山西农业科学,2008,36(12):20-22.ZHANG R J,SHI Y,MU Z X,et al. Present situation of soybean breeding and development countermeasures[J]. Journal of Shanxi Agricultural Sciences,2008,36(12):20-22.
    [5] IAEA. IAEA mutant database[DB/OL].[2018-04-09]. http://mvd. iaea. org/.
    [6] NOVAK F J,BRUNNER H. Plant breeding:Induced mutation technology for crop improvement[J]. IAEA Bulletin,1992,4:25-33.
    [7]杨兆民,张璐.辐射诱变技术在农业育种中的应用与探析[J].基因组学与应用生物学,2011,30(1):87-91.YANG Z M,ZHANG L. Application and analysis of radiation mutation technology in agricultural breeding[J]. Genomics and Applied Biology,2011,30(1):87-91.
    [8]黄桂丹.60Co-γ射线辐射育种研究进展[J].林业与环境科学,2016,32(2):107-111.HUANG G D. Research progress for60Co-γray radiation breeding[J]. Forestry and Environmental Science,2016,32(2):107-111.
    [9] STADLER L J. Mutations in barley induced by X-rays and radium[J]. Science,1928,68(1756):186-187.
    [10]王玫,翁秀英.大豆诱变育种的研究进展及动向[J].核农学通报,1987(2):1-4.WANG M,WENG X Y. Research progress and trend of soybean mutation breeding[J]. Nuclear Agricultural Bulletin,1987(2):1-4.
    [11] OLADOSU Y,RAFII M Y,ABDULLAH N,et al. Principle and application of plant mutagenesis in crop improvement:A review[J]. Biotechnologyand Biotechnological Equipment,2016,30(1):1-16.
    [12]陈丽霞,杜吉到,费志宏,等.诱变育种技术在大豆育种中的应用[J].大豆科学,2008,27(5):874-878.CHEN L X,DU J D,FEI Z H,et al. Induced mutation technique and the application on soybean breeding[J]. Soybean Science,2008,27(5):874-878.
    [13]谢皓,陈学珍,韩世文,等.60Co-γ射线辐照剂量对大豆早期世代致变效应的研究[J].华北农学报,2008(4):89-93.XIE H,CHEN X Z,HAN S W,et al. Study on effect of60Co-γrays irradiate dosage for induced mutation in early generations of soybean[J]. Acta Agriculturae Boreali-Sinica,2008(4):89-93.
    [14]余丽霞,李文建,杜艳,等.碳离子束辐照大豆当代诱变效应及褐皮突变体的初步研究[J].辐射研究与辐射工艺学报,2014,32(2):02040.YU L X,LI W J,DU Y,et al. Preliminary study on current mutagenic effects and brown seed mutant of soybean induced by carbon ion irradiation[J]. Journal of Radiation Research and Radiation Processing,2014,32(2):02040.
    [15]张秋英,余丽霞,李彦生,等.重离子束辐照大豆籽粒当代效应的初步研究[J].大豆科学,2013,32(5):587-590.ZHANG Q Y,YU L X,LI Y S,et al. Preliminary investigation of acceptable heavy ion beam irradiation dosage treated to soybean(Glycine max L.)seed[J]. Soybean Science,2013,32(5):587-590.
    [16]陈学珍,谢皓,燕平,等.大豆种子辐照处理后早期世代农艺性状的综合评价及遗传变异[J].分子植物育种,2004,2(1):60-69.CHEN X Z,XIE H,YAN P,et al. Phenotypes and genetic variation of agronomic traits of soybean irradiated byγray[J]. Molecular Plant Breeding,2004,2(1):60-69.
    [17] ADITYA K,VERMA N,SRIVASTAVA N,et al. Effect of gamma rays on seed germination,plant survival and quantitative characters on two varieties of soybean(Glycine Max.(L.)Merrill.)in M1 Generation[J] Asian Journal of Science and Technology,2017,8(11):6763-6768.
    [18]陈学珍,谢皓,栾涛.60Co-γ射线辐照处理后大豆M2农艺性状相关分析[J].北京农学院学报,2001,16(3):13-17.CHEN X Z,XIE H,LUAN T. Relationship of the agronomic character in M2 of soybean treated by60Co-γray[J]. Journal of Beijing Agricultural College,2001,16(3):13-17.
    [19]陈洪文,孙光祖.我省农作物辐射育种主要成就及其展望[J].黑龙江农业科学,1979,1(2):11-14.CHEN H W,SUN G Z,Major achievements and prospects of radiation crop breeding in our province[J]. Heilongjiang Agricultural Sciences,1979,1(2):11-14.
    [20]宋晓峰.大豆叶形突变体的遗传和功能分析[D].济南:山东师范大学,2016.SONG X F. The genetic and function analysis of soybean leaf shape mutant[D]. Jinan:Shandong Normal University,2016.
    [21]韩锁义.大豆突变体库的构建及子叶折叠突变相关基因的初步研究[D].南京:南京农业大学,2008.HAN S Y. Construction of mutant populationin soybean(Glycine max(L.)Merr.)and study on curled-cotyledon mutation related genes[D]. Nanjing:Nanjing Agriculture University,2008.
    [22]李元龙.大豆矮化短柄突变体baf6表型分析与基因定位[D].杨凌:西北农林科技大学,2017.LI Y L. The phenotypic analysis and gene location of dwarf and short petiole soybean mutant baf6[D]. Yangling:Northwest Agriculture and Forestry University,2017.
    [23]王亚琪,简朴,费云燕,等.大豆2个种皮不完整突变体的形态特点与遗传分析[J].核农学报,2017,31(4):621-626.WANG Y Q,JIAN P,FEI Y Y,et al. Morphological characters and inheritance of incomplete seed coat in two induced soybean mutants[J].Journal of Nuclear Agricultural Sciences,2017,31(4):621-626.
    [24] HA B K,LEE K J,VELUSAMY V,et al. Improvement of soybean through radiation-induced mutation breeding techniques in Korea[J].Plant Genetic Resources,2014,12(S1):S54-S57.
    [25]郭宁,郑佳佳,胡博,等.菜豆品种黄金勾伽玛射线突变体库的建立及突变表型观察[J].土壤与作物,2018,7(2):168GUO N,ZHENG J J,HU B,et al. Construction and phenotypic observation of a gamma radiation mutant library of common bean cultivar Golden Hook[J]. Soils and Crops,2018,7(2):168-176.
    [26]吴春雷,翟红,吴红艳,等.大豆极早熟品种60Coγ射线突变体库的建立及突变表型鉴定[J].大豆科学,2014,33(6):820-825.WU C L,ZHAI H,WU H Y,et al. Identify of mutant phynotype and construction of60Coγmutant population for soybean extremely early maturing cultivar[J]. Soybean science,2014,33(6):820-825.
    [27]孔福全.重离子致DNA损伤及修复的研究[D].天津:河北工业大学,2005.KONG F Q. Study of lesion and repair of DNA after irradiating by heavy ions[D]. Tianjin:Hebei University of Technology,2005.
    [28]李蕊.不同剂量12C重离子辐射对燕麦的诱变效应[D].兰州:兰州大学,2014.LI R. Mutagenic effects of different doses of12C heavy ion radiation on oat[D]. Lanzhou:Lanzhou University,2014.
    [29]王曾珍,陈太祥,白史且,等.三种诱变方式对普那菊苣M1代和SP1代各项生理指标的影响[J].西南农业学报,2010,31(8):1-10,21.WANG Z Z,CHEN T X,BAI S Q,et al. Mutation effect of three different wayson physiological index of M1and SP1of Pune chrysanthemum[J]. Journal of Grassland and Forage Science,2010,31(8):1-10,21.
    [30]刘智全,谷卫彬,李文建.重离子束辐照对甜高粱幼苗存活率及抗氧化酶活性的影响[J].安徽农业科学,2012,40(25):12454-12456,12604.LIU Z Q,GU W B,LI W J. Effects of heavy-ion beam irradiation on survival rate and antioxidant enzymes of sweet sorghum seedlings[J].Journal of Anhui Agricultural Sciences,2012,40(25):12454-12456,12604.
    [31]李群,顾瑞琦. X射线辐射对小麦幼苗生长及一些抗氧化酶活性的影响[J].辐射研究与辐射工艺学报,1997,15(3):32-36.LI Q,GU R Q. Effect of X-irradiation on the growth and some antioxidase activity in wheat seedlings[J]. Journal of Radiation Research and Radiation Processing,1997,15(3):32-36.
    [32]梁俊青.60Coγ辐照、12C6+离子束辐照和氮离子注入对小麦苗期生物学效应的研究[D].郑州:郑州大学,2017.LIANG J Q. Study on the biological effects of60Coγirradiation,12C6+ion beam irradiation and nitrogen ion implantation on wheat seedling[D]. Zhengzhou:Zhengzhou University,2017.
    [33]杜世章,刘婷婷,代其林,等.辐照对烟草抗氧化酶活性的影响[J].中国农业科技导报,2012,14(1):72-75.DU S Z,LIU T T,DAI Q L,et al. Effects of60Co-γray irradiation on antioxidant enzyme activities in tobacco[J]. Journal of Agricultural Science and Technology,2012,14(1):72-75.
    [34] ALIKAMANOGLU S,YAYCILI O,SEN A. Effect of gamma radiation on growth factors,biochemical parameters,and accumulation of trace elements in soybean plants(Glycine max L. Merrill)[J]. Biological Trace Element Research,2011,141(1-3):283-293.
    [35] TEWARI K,KUMARI S,VINUTHA T,et al. Gamma irradiation induces reduction in the off-flavour generation in soybean through enhancement of its antioxidant potential[J]. Journal of Radioanalytical and Nuclear Chemistry,2015,303(3):2041-2051.
    [36] MOUSSA H. Low dose of gamma irradiation enhanced drought tolerance in soybean[J]. Bulgarian Journal of Agricultural Science,2011,17(1):63-72.
    [37]隋丽.高LET辐射致DNA和生物体损伤的机制及其防护研究[D].北京:中国原子能科学研究院,2006.SUI L. Investigation of mechanism of DNA or organism damage induced by high LET radiation and corresponding radioprotection[D]. Beijing:China Institute of Atomic Energy,2006.
    [38]强维亚,汤红官,侯宗东,等.增强UV-B辐射对大豆胚轴DNA损伤、修复和蛋白质含量的影响[J].生态学报,2004,24(4):852-856.QIANG W Y,TANG H G,HOU Z D,et al. Effect of enhanced UV-B radiation on DNA damage,repair and protein content in soybean hypocotyls[J]. Acta Ecologica Sinica,2004,24(4):852-856.
    [39] LACKEY J A. Chromosome numbers in the Phaseoleae(Fabaceae:Faboideae)and their relationship to taxonomy[J]. American Journal of Botany,1980,67(4):595-602.
    [40] SCHMUTZ J,CANNON S B,SCHLUETER J,et al. Genome sequence of the palaeopolyploid soybean[J]. Nature,2010,463(7278):178-183.
    [41]夏正俊.大豆基因组解析与重要农艺性状基因克隆研究进展[J].植物学报,2017,52(2):148-158.XIA Z J. Research progress in whole-genome analysis and cloning of genes underlying important agronomic traits in soybean[J]. Chinese Bulletin of Botany,2017,52(2):148-158.
    [42] TANHINDARTO R P,HARIYADI P,PURNOMO E H,et al. Effects of gamma irradiation at different combinations of dose-rate and time of exposure on the isoflavone contents of soybean[J]. Asian Journal of Food and Agro-Industry,2013,6(6):322-328.
    [43] PATIL A,TAWARE S P,OAK M D,et al. Improvement of oil quality in soybean[Glycine max(L.)Merrill]by mutation breeding[J].Journal of the American Oil Chemists'Society,2007,84(12):1117-1124.
    [44]李多芳,曹天光,耿金鹏,等.电离辐射致植物诱变效应的损伤-修复模型[J].物理学报,2015,64(24):415-422.LI D F,CAO T G,GENG J P,et al. Damage-repair model for mutagenic effects of plant induced by ionizing radiation[J]. Acta Physica Sinica,2015,64(24):0248701.
    [45]薛云云.60Co诱变(晋大78)后代遗传性状变异分析[D].晋中:山西农业大学,2013.XUE Y Y. The analysis of the heredity character variance of the progeny(of Jinda 78)irradiated by60Co-ray[D]. Jinzhong:Shanxi Agricultural University,2013.
    [46]谷秀芝,翁秀英.大豆辐射与杂交相结合后代选择方法的研究[J].核农学通报,1990,11(6):254-258.GU X Z,WENG X Y. Soybean radiation hybrid offspring combined selection method[J]. Journal of Nuclear and Agronomy Science,1990,11(6):254-258.
    [47]尚娜. EMS诱变番茄突变体库的构建及表型分析[D].哈尔滨:东北农业大学,2017.SHANG N. Construction and phenotypic analysis of EMS mutagenesis tomato mutant[D]. Harbin:Northeast Agricultural University. 2017.
    [48] ARASE S,HASE Y,ABE J,et al. Optimization of ion-beam irradiation for mutagenesis in soybean:Effects on plant growth and production of visibly altered mutants[J]. Plant Biotechnology,2011,28(3):323-329.
    [49]邱丽娟,李英慧,关荣霞,等.大豆核心种质和微核心种质的构建、验证与研究进展[J].作物学报,2009,35(4):571-579.QIU L J,LI Y H,GUAN R X,et al. Establishment,representative testing and research progress of soybean core collection and mini core collection[J]. Acta Agronomica Sinica,2009,35(4):571-579.
    [50]杨柳,张彬彬,韩英鹏,等.大豆亚麻酸含量的QTL分析[J].大豆科学,2006,25(3):270-274,278.YANG L,ZHANG B B,HAN Y P,et al. The study on the screening of transgene soybean by way of pollen-tube using kanamycin[J]. Soybean Science,2006,25(3):270-274,278.
    [51]王培英,翁秀英,王彬如,等.大豆诱变育种研究四十年[J].激光生物学报,1998,7(3):52-55.WANG P Y,WENG X Y,WANG B R,et al. Study on soybean mutation breeding for 40 years[J]. Acta Laser Biology Sinica,1998,7(3):52-55.
    [52]李雪华.大豆突变体库的初步构建及突变类型的鉴定[D].南京:南京农业大学,2003.LI X H. Construction of soybean mutant pool sand characterization of different mutant-types[D]. Nanjing:Nanjing Agricultural University,2003.
    [53] BOLON Y T,HAUN W J,XU W W,et al. Phenotypic and genomic analyses of a fast neutron mutant population resource in soybean[J].Plant Physiology,2011,156(1):240-253.
    [54]杜维广,盖钧镒.大豆超高产育种研究进展的讨论[J].土壤与作物,2014,3(3):81-92.DU W G,GAI J Y. A discussion on advances in breeding for super high-yielding soybean cultivars[J]. Soils and Crops,2014,3(3):81-92.
    [55]翁秀英,王彬如,吴和礼,等.大豆辐射育种的研究[J].遗传学报,1974,1(2):157-169.WENG X Y,WANG B R,WU H L,et al. Study on soybean radiation breeding[J]. Journal of Genetics and Genomics,1974,1(2):157-169.
    [56]鹿文成,闫洪睿,张雷,等.60Co-γ射线诱变选育早熟和高产及优质大豆新品种黑河52[J].黑龙江农业科学,2011(9):4-6.LU W C,YAN H R,ZHANG L,et al. Breeding of soybean cultivar heihe 52 with early maturity,high yield and good quality by hybridization and60Coγ-ray irradiation[J]. Heilongjiang Agricultural Sciences,2011(9):4-6.
    [57]孟继武,胡亚坤,郑荣儿.激光诱导优质高产大豆育种[J].激光生物学报,2001,10(3):215-217.MENG J W,HU Y K,ZHENG R E. The breeding of high yield soybean with laser irradiation[J]. Acta Laser Biology Sinica,2001,10(3):215-217.
    [58]孙玉,姜永平,刘军民.我国大豆辐射诱变育种研究进展与展望[J].山东农业科学,2008,40(1):14-17.SUN Y,JIANG Y P,LIU J M. Research progress and prospect of soybean radiation mutation breeding in China[J]. Shandong Agricultural Sciences,2008,40(1):14-17.
    [59]张燕玲,吴立蓉,贺红.人工诱变技术在植物抗病育种中的应用(综述)[J].亚热带植物科学,2007,36(3):69-73.ZHANG Y L,WU L R,HE H. Application and prospect of artificial mutation technology on breeding for resistance to the diseases of plants[J]. Subtropical Plant Science,2007,36(3):69-73.
    [60]吕秀珍,刘忠堂,郭泰,等.辐射选育抗灰斑病兼抗病毒病的大豆新品种合丰33号[J].核农学通报,1996,17(2):51-52.LX Z,LIU Z T,GU T,et al. Radiation breeding of new soybean variety hefeng 33 against gray leaf spot and virus resistance[J]. Acta Agriculturae Nucleatae Sinica,1996,17(2):51-52.
    [61]吴昆.高蛋白大豆牡丰6号生育特点及栽培技术[J].农业科技通讯,1991,21(3):13.WU K. Fertility characteristics and cultural techniques of high protein soybean variety,mufeng 6[J]. Bulletin of Agricultural Science and Technology,1991,21(3):13.
    [62]仝本英,李恒杰.大豆诱变30及其高产栽培技术[J].河南农业科学,1989,16(11):3-4.TONG B Y,LI H J. Soybean mutation 30 high yield cultivation technique[J]. Journal of Henan Agricultural Sciences,1989,16(11):3-4.
    [63]孙维琦.大豆提取物对辐射损伤保护作用的研究[D].长春:吉林大学,2007.SUN W Q. Study on the protective effects of soybean extracton radiation-injured mice[D]. Changchun:Jilin University,2007.
    [64] TANAKA A,SHIKAZONO N,HASE Y. Studies on biological effects of ion beams on lethality,molecular nature of mutation,mutation rate,and spectrum of mutation phenotype for mutation breeding in higher plants[J]. Journal of Radiation Research,2010,51(3):223-233.
    [65]杜艳.碳离子束辐照拟南芥突变体筛选及诱变效应研究[D].北京:中国科学院大学,2015.DU Y. Mutation screening and mutagenic effects research of carbon-ion irradiation on Arabidopsis thaliana[D]. Beijing:University of Chinese Academy of Sciences,2015.
    [66]郑伟,郭泰,王志新,等.大豆航天育种研究进展[J].辐射研究与辐射工艺学报,2015,33(5):050101.ZHENG W,GUO T,WANG Z X,et al. Research progress on the space-flight mutation breeding of soybean[J]. Journal of Radiation Research and Radiation Processing,2015,33(5):050101.
    [67]张文涛,杜久元,白斌.作物空间诱变及其在遗传育种中的应用[J].种子,2014,33(4):48-52.ZHANG W T,DU J Y,BAI B. Aerospace induced mutations for crop genetics and breeding[J]. Seed,2014,33(4):48-52.

© 2004-2018 中国地质图书馆版权所有 京ICP备05064691号 京公网安备11010802017129号

地址:北京市海淀区学院路29号 邮编:100083

电话:办公室:(+86 10)66554848;文献借阅、咨询服务、科技查新:66554700