基于丙氨酸为底物的厌氧氨氧化过程研究
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摘要
通过批次实验,分别研究了丙氨酸对于厌氧氨氧化过程的短期以及长期影响.研究表明,当丙氨酸为唯一底物时,无论短期还是长期培养,厌氧氨氧化过程均受到很大影响,体系内未发生氮的去除.在缺乏电子受体NO_2~--N的情况下,体系内未发生厌氧氨氧化反应,虽然丙氨酸降解率达到了86%以上,但产生的NO_4~+-N在体系内积累.当向丙氨酸为底物的系统中投加NO_4~+-N和NO_2~--N时,短期(7h)培养中厌氧氨氧化活性受到的影响较小.同时,2mmol/L丙氨酸在厌氧氨氧化体系中10h即可去除78%,高浓度(10mmol/L)丙氨酸在60h达到99%的去除率;长期培养过程中,浓度为2mmol/L的丙氨酸一定程度上抑制了厌氧氨氧化活性.在厌氧氨氧化与反硝化的共同作用下,TN的去除率达到57%,丙氨酸的去除率为99%左右.
The short-term and long-term effects of alanine on ANAMMOX process were investigated by batch experiments. The ANAMMOX process was greatly affected both in short-term and long-term culture when alanine was the sole substrate, and there was no nitrogen removal in the system. Without electron acceptor NO_2~-N, ANAMMOX process could not occur in the system, although the alanine removal could reach more than 86%, NO_4~+-N was accumulated in the system. When NO_4~+-N and NO_2~--N were added to the system with alanine as a substrate, the activity of ANAMMOX bacteria was not affected so much in the short term(7h), and 2mmol/L and 10mmol/L alanine reached 78% and 99% removal efficiencies in 10 and 60 h, respectively. During the long term experiments, the activity of ANAMMOX bacteria could be inhibited by alanine with a concentration of 2mmol/L. Combined with ANAMMOX and denitrification processes, the removal efficiency of TN reached 57%, and the removal efficiency of alanine was about 99%.
The short-term and long-term effects of alanine on ANAMMOX process were investigated by batch experiments. The ANAMMOX process was greatly affected both in short-term and long-term culture when alanine was the sole substrate, and there was no nitrogen removal in the system. Without electron acceptor NO_2~-N, ANAMMOX process could not occur in the system, although the alanine removal could reach more than 86%, NO_4~+-N was accumulated in the system. When NO_4~+-N and NO_2~--N were added to the system with alanine as a substrate, the activity of ANAMMOX bacteria was not affected so much in the short term(7h), and 2mmol/L and 10mmol/L alanine reached 78% and 99% removal efficiencies in 10 and 60 h, respectively. During the long term experiments, the activity of ANAMMOX bacteria could be inhibited by alanine with a concentration of 2mmol/L. Combined with ANAMMOX and denitrification processes, the removal efficiency of TN reached 57%, and the removal efficiency of alanine was about 99%.
引文
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[23]魏思佳,于德爽,李津,等.厌氧氨氧化与反硝化耦合脱氮除碳研究Ⅰ:COD/NH4+-N对耦合反应的影响[J].中国环境科学,2016,36(3):759-767.
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[26]Jin R C,Yang G F,Yu J J,et al.The inhibition of the Anammox process:a review[J].Chemical Engineering Journal,2012,197:67-79.
[2]Strous M,Heijnen J J,Kuenen J G,et al.The sequencing batch reactor as a powerful tool for the study of slowly growing anaerobicammonium oxidizing microorganisms[J].Appl.Microbiol.Biotechnol.,1998,50(5):589-596.
[3]曹天昊,王淑莹,苗蕾,等.不同基质浓度下SBR进水方式对厌氧氨氧化的影响[J].中国环境科学,2015,35(8):2334-2341.
[4]王全,张克峰,王洪波,等.厌氧氨氧化技术研究进展[J].山东建筑大学学报,2011,26(1):80-83.
[5]蒋彬,吕锡武.生物脱氮除磷机理及技术研究[J].安全与环境工程,2005,12(3):74-77.
[6]高大文,侯国凤,陶彧,等.厌氧氨氧化菌代谢有机物研究[J].哈尔滨工业大学学报,2012,44(2):89-93.
[7]于英翠,高大文,陶彧,等.利用序批式生物膜反应器启动厌氧氨氧化研究[J].中国环境科学,2012,32(5):843-849.
[8]Kartal B,Rattray J,van Niftrik L A,et al.Candidatus"Anammoxoglobus propionicus"a new propionate oxidizing species of anaerobic ammonium oxidizing bacteria[J].Systematic and Applied Microbiology,2007,30(1):39-49.
[9]Huang X L,Gao D W,Tao Y,et al.C2/C3 fatty acid stress on anammox consortia dominated by Candidatus Jettenia asiatica[J].Chemical Engineering Journal,2014,253:402-407.
[10]Guven D,Dapena A,Kartal B,et al.Propionate oxidation by and methanol inhibition of anaerobic ammonium-oxidizing bacteria[J].Applied and Environmental Microbiology,2005,71(2):1066-1071.
[11]李良红,陈武,梅平.氨基酸废水处理技术的研究进展[J].氨基酸和生物资源,2006,28(2):63-66.
[12]雷智平.双极膜电渗析技术处理氨基酸废水的研究[D].太原:太原理工大学,2003.
[13]贾晓波.膜生物反应器处理氨基酸生产废液的研究[D].天津:天津大学,2007.
[14]陆林华.利用胱氨酸废水制备工业酸洗缓蚀剂[J].化工环保,2002,22(4):241-242.
[15]张开诚.利用胱氨酸废液生产复合氨基酸营养粉作饲料添加剂[J].饲料研究,2000,(7):30-31.
[16]叶伟.厌氧生物法处理氨基酸发酵废水的实验研究[J]..工业水处理,2004,24(1):11-13.
[17]丛岩,黄晓丽,王小龙,等.厌氧氨氧化颗粒污泥的快速形成[J].化工学报,2014,65(2):664-671.
[18]魏启航,王小龙,李龙伟,等.盐度对厌氧氨氧化脱氮效能的影响[J].工业水处理,2016,36(4):25-29.
[19]郑重,孙琦,石永伟,等.高效液相色谱-串联质谱法直接定量分析植物酵素中多种氨基酸成分[J].色谱,2015,33(3):309-313.
[20]胡勇有,梁辉强,朱静平,等.有机碳源环境下的厌氧氨氧化批式实验[J].华南理工大学学报:自然科学版,2007,35(6):710-715.
[21]杨洋,左剑恶,沈平,等.温度、p H值和有机物对厌氧氨氧化污泥活性的影响[J].环境科学,2006,27(4):691-695.
[22]刘金苓,钟玉鸣,谢志儒,等.厌氧氨氧化微生物在有机碳源条件下的代谢特性[J].环境科学学报,2009,29(10):2041-2047.
[23]魏思佳,于德爽,李津,等.厌氧氨氧化与反硝化耦合脱氮除碳研究Ⅰ:COD/NH4+-N对耦合反应的影响[J].中国环境科学,2016,36(3):759-767.
[24]Schalk J,Oustad H,Kuenen J G,et al.The anaerobic oxidation of hydrazine:a novel reaction in microbial nitrogen metabolism[J].FEMS Microbiology Letters,1998,158(1):61-67.
[25]Fern Ndez I,Dosta J,Fajardo C,et al.Short-and long-term effects of ammonium and nitrite on the Anammox process[J].Journal of Environmental Management,2012,95:S170-S174.
[26]Jin R C,Yang G F,Yu J J,et al.The inhibition of the Anammox process:a review[J].Chemical Engineering Journal,2012,197:67-79.