重金属对硫酸盐还原菌影响
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摘要
含重金属硫酸盐废水是我国工业水污染的突出问题,利用硫酸盐还原菌的生物去除重金属的方法具有投资少、成本低、能耗少、去除率高,没有二次污染等优点而成为研究的热点。文章以混合培混养物作为接种污泥,考察不同浓度的重金属离子(Cu2+、Cd2+、Ni2+、Hg2+)对硫酸盐还原菌(Sulfate reducing bacteria,SRB)的抑制作用。研究表明:10 mg/L的Cu2+、Cd2+和20 mg/L的Hg2+对SRB还原硫酸盐的影响较小,硫酸盐最大去除率可分别达到94.1%、94.6%、91.3%,与空白(93.9%)相近;20 mg/L的Cu2+对SRB的抑制最为强烈,硫酸盐最大还原率仅为48.2%,剩余金属离子(Cd2+、Ni2+、Hg2+)都分别随着浓度的增大而对SRB的抑制作用增强;相同浓度的重金属离子对SRB的抑制顺序为Ni2+>Cu2+>Cd2+>Hg2+,抑制浓度分别为10、20、30、60 mg/L。最后阐述了各个反应器中硫酸盐还原率最大时,(WCOD/WSO42-)与硫酸盐还原率的关系。
This paper related to a bench scale experiment for treatment of wastewater containing both sulfate and heavy metals ions of Cu2+,Cd2+,Ni2+ and Hg2+ with emphasis on influences of heavy metals on sulfate-reduction bacteria(SRB)in the anaerobic activated sludge system(UASB reactor).The experiment presented the maximum sulfate reduction rates-all above 91%-when affected by Cu2+,Cd2+ and Hg2+ of limited concentrations respectively;the strongest inhibitory effect exerted by heavy metal ions,e.g.copper ion,when its concentration rose to as high as 20 mg/L,as a result,the sulfate reduction rate was brought down to 48.2%.According to the experiment,inhibitory rating of the above heavy metals was Ni >Cu>Cd>Hg.In addition,due to the fact that COD in the wastewater sample decreased when sulfate being removed,relationship between COD reduction and sulfate reduction rate (WCOD/WSO42-) was studied in this paper.
This paper related to a bench scale experiment for treatment of wastewater containing both sulfate and heavy metals ions of Cu2+,Cd2+,Ni2+ and Hg2+ with emphasis on influences of heavy metals on sulfate-reduction bacteria(SRB)in the anaerobic activated sludge system(UASB reactor).The experiment presented the maximum sulfate reduction rates-all above 91%-when affected by Cu2+,Cd2+ and Hg2+ of limited concentrations respectively;the strongest inhibitory effect exerted by heavy metal ions,e.g.copper ion,when its concentration rose to as high as 20 mg/L,as a result,the sulfate reduction rate was brought down to 48.2%.According to the experiment,inhibitory rating of the above heavy metals was Ni >Cu>Cd>Hg.In addition,due to the fact that COD in the wastewater sample decreased when sulfate being removed,relationship between COD reduction and sulfate reduction rate (WCOD/WSO42-) was studied in this paper.
引文
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[9]Oliver J H,Li H,Chen J M.Effects of metal additions on sulfate reduction activity in wastewaters[J].Toxicological and Environmental Chemistry,1994,46:197-212.
[10]Hao O J,Huang L,Chen J M,et al.Effects of metal ad-ditions on sulfate reduction activity in wastewaters[J].Toxi-col Environ Chem,1994,46(4):197-212.
[11]Aksu Z,Sag Y,Kutsal T.The biosorption of coppe(rⅡ)by vulguris and ramigera[J].Environ Technol,1992,13(3):579-590.
[12]Poulson S R,Colberg P J,Drever J I.Toxicity of heavy metals Ni,Zn to Desulfovibrio desulfuricans[J].Geomicro-biol,1997(14):41-49.
[13]程为,辛宝平,藿祥明,等.生物还原-化学沉淀去除烟气SO2中Na2SO3和硫化物的研究[J].环境化学,2005,24(5):506-509.Cheng Wei,Xin Bao-ping,Huo Xiang-ming,et al.Re-moval of SO 2 by combined processes of microbial deoxi-dization and chemical precipitation[J].Environmental Chem-istry,2005,24(5):506-509.(in Chinese)
[2]Cetin D,Donmez S,Donmez G.The treatment of textile wastewater including chromium(Ⅵ)and reactive dye by sulfate reducing bacterial enrichment[J].Journal of Envi-ronmental Management,2008,88(1):76-82.
[3]Yi Z,Lian B,Yang Y Q,et al.Treatment of simulated wastewater from in situ leaching uranium mining by ze-rovaent iron and sulfate reducing bacteria[J].Transactions of Nonferrous Metals Society of China,2009,19:840-844.
[4]刘恢,柴立元,闵小波,等.活性污泥处理重金属废水的研究进展[J].工业用水与废水,2004,35(4):9-12.Liu-hui,Chai Li-yuan,Min Xiao-bo,et al.Study and development of activated-sludge treatment of heavy metal-containing wastewater[J].Industrial Water&Wasterwater,2004,35(4):9-12.(in Chinese)
[5]Teclu D,Tivchev G,Laing M,et al.Determination of the elemental composition of molasses and its suitability as car-bon source for growth of sulphate reducing bacteria[J].Journal of Hazardous Materials,2009,161(2-3):1157-1165.
[6]Costa M C,Santos E S,Barros R J,et al.Wine wastes as carbon source for biological treatment of acid mine drainage[J].Chemo sphere,2009,75:831-836.
[7]魏复胜,刘文启,王心芳,等.水和废水监测分析方法[M].第四版.北京:中国环境科学出版社,2002:120-165.Wei Fu-sheng,Liu Wen-qi,Wang Xin-fang,et al.Moni-toring Method of Water and Wastewater[M].Fourth Editon.Beijing:Chinese Envionmental Scenice Press,2002,120-165.(in Chinese)
[8]苏宇,王进,彭书传,等.以稻草和污泥为碳源硫酸盐还原菌处理酸性矿山排水[J].环境科学,2010,31(8):1858-1863.Su Yu,Wang Jin,Peng Shu-chuan,et al.Rice straw and sewage sludge as carbon sources for sulfate-reducing bacte-ria treating acid mine drainage[J].Environmental Science,2010,31(8):1858-1863.(in Chinese)
[9]Oliver J H,Li H,Chen J M.Effects of metal additions on sulfate reduction activity in wastewaters[J].Toxicological and Environmental Chemistry,1994,46:197-212.
[10]Hao O J,Huang L,Chen J M,et al.Effects of metal ad-ditions on sulfate reduction activity in wastewaters[J].Toxi-col Environ Chem,1994,46(4):197-212.
[11]Aksu Z,Sag Y,Kutsal T.The biosorption of coppe(rⅡ)by vulguris and ramigera[J].Environ Technol,1992,13(3):579-590.
[12]Poulson S R,Colberg P J,Drever J I.Toxicity of heavy metals Ni,Zn to Desulfovibrio desulfuricans[J].Geomicro-biol,1997(14):41-49.
[13]程为,辛宝平,藿祥明,等.生物还原-化学沉淀去除烟气SO2中Na2SO3和硫化物的研究[J].环境化学,2005,24(5):506-509.Cheng Wei,Xin Bao-ping,Huo Xiang-ming,et al.Re-moval of SO 2 by combined processes of microbial deoxi-dization and chemical precipitation[J].Environmental Chem-istry,2005,24(5):506-509.(in Chinese)