苦碟子总黄酮对东莨菪碱诱导大鼠学习记忆损害的影响
|
摘要
目的:观察苦碟子总黄酮(TFIS)对东莨菪碱所致大鼠记忆获得障碍模型的影响。方法:120只大鼠随机分为正常对照组,模型组,盐酸多奈哌齐组,TFIS低剂量组(50 mg/kg)、中剂量组(100 mg/kg)、高剂量组(200 mg/kg),每天灌胃1次,连续给予7 d,对照组和模型组给予同体积的蒸馏水。各组以跳台法和水迷宫法测试大鼠学习记忆的能力。结果:跳台实验结果显示:与模型组比较,TFIS中、高剂量组跳台错误次数明显降低(P<0.05),潜伏期明显延长(P<0.05)。水迷宫试验结果显示:与模型组比较,TFIS中、高剂量组达到平台的潜伏期及达到平台的游程明显缩短(P<0.05),在2 min内穿越平台的次数,在平台区的逗留时间有增加趋势。结论:苦碟子总黄酮可以有效改善东莨菪碱所致的学习记忆障碍大鼠的学习记忆能力。
Objective: To study the memory-promoting effects of Total Flavonoids from Ixeris Sonchifolia(Bae.)Hance(TFIS) on rats. Methods:120 rats were randomly divided into blank control group, model group, Donepezil HCl group, TFIS three dose group(50,100,200 mg/kg) and daily administration of stomach,gastric gavage for 7 days. The leaning and memory ability was estimated by jumping test and maze test in rar. Results:Compared with model group, medium and high dose group prolonged the latency periods of memory and significally reduced the error times in impairment groups(P<0.05) in jumping test. Compared with model group,medium and high dose group sinificantly shortened the model rats escaping latent period, increased the frequency of crossing-over(P<0.05). Conclusion: TFIS can improve the learning and memory ability of rats induced by scopolamine.
Objective: To study the memory-promoting effects of Total Flavonoids from Ixeris Sonchifolia(Bae.)Hance(TFIS) on rats. Methods:120 rats were randomly divided into blank control group, model group, Donepezil HCl group, TFIS three dose group(50,100,200 mg/kg) and daily administration of stomach,gastric gavage for 7 days. The leaning and memory ability was estimated by jumping test and maze test in rar. Results:Compared with model group, medium and high dose group prolonged the latency periods of memory and significally reduced the error times in impairment groups(P<0.05) in jumping test. Compared with model group,medium and high dose group sinificantly shortened the model rats escaping latent period, increased the frequency of crossing-over(P<0.05). Conclusion: TFIS can improve the learning and memory ability of rats induced by scopolamine.
引文
[1] 戴锦娜,尹然,陈晓辉,等.苦碟子化学成分和药理作用研究进展[J].西北药学杂志,2006,21(4):94-96.
[2] 李金珍,袁杰,顾瑾. 苦碟子注射液在临床中的应用[J].中国医药指南,2010,8(1):44.
[3] 何林,蒲志勇,赵敏,等.苦碟子制剂注射液治疗急性脑梗塞32例疗效观察[J].西部医学,2007,19(4):587-589.
[4] 高杰.脑蛋白水解物联合苦碟子治疗血管性痴呆的临床效果分析[J].中国当代医药,2014,21(16):89-91.
[5] 徐叔云,卞如濂,陈修. 药理试验方法学[M].3 版,北京:人民卫生出版社,2002:826-830.
[6] 张小平,张文平,侯林,等. 醒脑益智胶囊对记忆获得障碍模型小鼠的学习记忆改善作用[J].时珍国医国药,2015,26(7):1598-1600.
[7] 刘瑾,刘永伟,李岩,等. 桑椹补脑膏对东莨菪碱所致记忆获得障碍小鼠学习记忆能力影响[J].辽宁中医药大学学报,2015,17(1):22-24.
[8] 周世月,邓之荣,谭琳,等. 五味子酚对东莨菪碱诱导的痴呆小鼠学习记忆损伤的保护作用[J].中国药学杂志,2014,49(23):2088-2091.
[9] 李裕倩,王英锋,时小礼,等. 益智仁乙醇提取物对东莨菪碱痴呆小鼠学习记忆的影响[J].首都师范大学学报,2016,36(1):54-56.
[10] 罗浩铭,韩威,王颖,等. 苦碟子注射液抗氧化成分的分离与活性评价研究[J].天然产物研究与开发,2013,25(5):672-675.
[11] 陈英红,张晓荧,罗浩铭,等. 苦碟子提取物中总黄酮含量测定方法的探讨[J].时珍国医国药,2012,23(2):313-315.
[12] 陈英红,姜翔之,罗浩铭,等. 苦碟子总黄酮提取工艺研究[J].特产研究,2011,33(1):29-30, 54.
[2] 李金珍,袁杰,顾瑾. 苦碟子注射液在临床中的应用[J].中国医药指南,2010,8(1):44.
[3] 何林,蒲志勇,赵敏,等.苦碟子制剂注射液治疗急性脑梗塞32例疗效观察[J].西部医学,2007,19(4):587-589.
[4] 高杰.脑蛋白水解物联合苦碟子治疗血管性痴呆的临床效果分析[J].中国当代医药,2014,21(16):89-91.
[5] 徐叔云,卞如濂,陈修. 药理试验方法学[M].3 版,北京:人民卫生出版社,2002:826-830.
[6] 张小平,张文平,侯林,等. 醒脑益智胶囊对记忆获得障碍模型小鼠的学习记忆改善作用[J].时珍国医国药,2015,26(7):1598-1600.
[7] 刘瑾,刘永伟,李岩,等. 桑椹补脑膏对东莨菪碱所致记忆获得障碍小鼠学习记忆能力影响[J].辽宁中医药大学学报,2015,17(1):22-24.
[8] 周世月,邓之荣,谭琳,等. 五味子酚对东莨菪碱诱导的痴呆小鼠学习记忆损伤的保护作用[J].中国药学杂志,2014,49(23):2088-2091.
[9] 李裕倩,王英锋,时小礼,等. 益智仁乙醇提取物对东莨菪碱痴呆小鼠学习记忆的影响[J].首都师范大学学报,2016,36(1):54-56.
[10] 罗浩铭,韩威,王颖,等. 苦碟子注射液抗氧化成分的分离与活性评价研究[J].天然产物研究与开发,2013,25(5):672-675.
[11] 陈英红,张晓荧,罗浩铭,等. 苦碟子提取物中总黄酮含量测定方法的探讨[J].时珍国医国药,2012,23(2):313-315.
[12] 陈英红,姜翔之,罗浩铭,等. 苦碟子总黄酮提取工艺研究[J].特产研究,2011,33(1):29-30, 54.