川椒方中挥发油的提取及包合物制备与表征
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摘要
目的优选川椒方中蛇床子、荆芥、川芎、花椒混合挥发油的最佳提取工艺,制备混合挥发油与β-环糊精(β-CD)的包合物并表征其形成。方法以收油率为指标,采用单因素试验优选挥发油提取工艺;以包封率为指标,采用饱和水溶液法制备包合物,通过单因素试验和正交试验优选包合工艺参数;采用显微镜法和红外分光光度法对包合物进行表征评价。结果挥发油提取工艺为:加8倍量水,浸泡2 h,提取5 h;包合工艺为:β-CD(g)与水(m L)的比例为1∶10,挥发油(m L)与β-CD(g)的比例为1∶10,在40℃条件下包合1.5 h。结论本研究优化筛选的提取包合工艺稳定可行,可用于川椒方的颗粒剂生产。
Objective To optimize the extraction process of mixed volatile oil of Cnidii Fructus, Schizonepetae Herba, Chuanxiong Rhizoma, Zanthoxyli Pericarpium of Chuanjiao Decoction; To prepare and identify the compound essential oil/β-cyclodextrin inclusion complex. Methods The extraction process of volatile oil was optimized by single factor experiment with the receiving rate as the index. The inclusion complex was prepared by the saturated aqueous solution method with the encapsulation efficiency as the index, and the inclusion process parameters were optimized by single factor test and orthogonal test. The inclusion complexes were characterized by microscopy and infrared spectrophotometry. Results The optimized extraction conditions were adding 8 fold water, soaking for 2 h, and extracting for 5 h. The optimized inclusion conditions were the β-cyclodextrin to water for 1:10(g/mL), volatile oil and β-cyclodextrin at ratio of 1:10(mL/g), inclusion temperature 40 ℃ for 1.5 h. Conclusion The optimal extraction and inclusion process are stable and feasible, which can be used for the granule production of Chuanjiao Decoction.
Objective To optimize the extraction process of mixed volatile oil of Cnidii Fructus, Schizonepetae Herba, Chuanxiong Rhizoma, Zanthoxyli Pericarpium of Chuanjiao Decoction; To prepare and identify the compound essential oil/β-cyclodextrin inclusion complex. Methods The extraction process of volatile oil was optimized by single factor experiment with the receiving rate as the index. The inclusion complex was prepared by the saturated aqueous solution method with the encapsulation efficiency as the index, and the inclusion process parameters were optimized by single factor test and orthogonal test. The inclusion complexes were characterized by microscopy and infrared spectrophotometry. Results The optimized extraction conditions were adding 8 fold water, soaking for 2 h, and extracting for 5 h. The optimized inclusion conditions were the β-cyclodextrin to water for 1:10(g/mL), volatile oil and β-cyclodextrin at ratio of 1:10(mL/g), inclusion temperature 40 ℃ for 1.5 h. Conclusion The optimal extraction and inclusion process are stable and feasible, which can be used for the granule production of Chuanjiao Decoction.
引文
[1]国家家药典委员会.中中华人民共和国药药典:一部[M].北北京:中国医药科科技出版版社,2015:315.
[2]刘明平,吴依娜,韦品清,等.蛇床子子止痒有效组分筛筛选及作用机制研研究[J]].中医药导报,20009,15(7):66-67.
[3]王凤凤,温桃群,桑文涛,等.荆芥挥发油化学成分及药理作用研究现状[J].中南药药学,2017,15(3):312-318.
[4]倪健健,董竞成.三种中药药效成分抗抗支气管哮喘变应应性炎症的实验研研究[J]].中国实验方剂学学杂志,2004,10(4):49-51.
[5]杜旌旌畅,谢晓芳,熊亮,等.川芎挥发发油的化学成分与药理活性研究进进展[J]].中国中药杂志,2016,41(23):44328-4333.
[6]梁辉辉,赵镭,杨静,等等.花椒化学成分分及药理作用的研研究进展[J].华西西药学杂杂志,2014,29(1):91-94.
[7]曾晓会,周瑞玲,陈玉兴,等.花椒超超临界萃取物治疗疗哮喘的药效学研研究[J]].中药材,2005,228(2):132-134.
[8]杨欢欢,詹雪艳,林宏英,等.中药挥发发油环糊精包合物物研究现状[J].中中国中医医药信息杂志,20015,22(10):129-133.
[9]杜杰杰峰,叶庆华,刘峻峻峰,等.4种植物物混合挥发油β--环糊精包合工艺艺的优化化及稳定性考察[[J].中国医药工业业杂志,2015,46(77):709-712.
[10]王王庆玲,倪健,张欣欣,等.桂芍子喘颗颗粒中挥发油包合合工艺及稳定性研研究[J]].现代中药研究与与实践,2017,31(5):48-51.
[11]郝郝晶晶,李海亮,龚龚慕辛.挥发油环糊精包合技术的研究进展及存在在问题分分析[J].中国实验验方剂学杂志,2013,19(2):352-3356.
[12]王王晓琴,曹礼,安玲玲,等.当归和党参参混合挥发油提取取和β-环糊精包包合工艺艺研究[J].中国酿酿造,2017,36(9):153-157.
[2]刘明平,吴依娜,韦品清,等.蛇床子子止痒有效组分筛筛选及作用机制研研究[J]].中医药导报,20009,15(7):66-67.
[3]王凤凤,温桃群,桑文涛,等.荆芥挥发油化学成分及药理作用研究现状[J].中南药药学,2017,15(3):312-318.
[4]倪健健,董竞成.三种中药药效成分抗抗支气管哮喘变应应性炎症的实验研研究[J]].中国实验方剂学学杂志,2004,10(4):49-51.
[5]杜旌旌畅,谢晓芳,熊亮,等.川芎挥发发油的化学成分与药理活性研究进进展[J]].中国中药杂志,2016,41(23):44328-4333.
[6]梁辉辉,赵镭,杨静,等等.花椒化学成分分及药理作用的研研究进展[J].华西西药学杂杂志,2014,29(1):91-94.
[7]曾晓会,周瑞玲,陈玉兴,等.花椒超超临界萃取物治疗疗哮喘的药效学研研究[J]].中药材,2005,228(2):132-134.
[8]杨欢欢,詹雪艳,林宏英,等.中药挥发发油环糊精包合物物研究现状[J].中中国中医医药信息杂志,20015,22(10):129-133.
[9]杜杰杰峰,叶庆华,刘峻峻峰,等.4种植物物混合挥发油β--环糊精包合工艺艺的优化化及稳定性考察[[J].中国医药工业业杂志,2015,46(77):709-712.
[10]王王庆玲,倪健,张欣欣,等.桂芍子喘颗颗粒中挥发油包合合工艺及稳定性研研究[J]].现代中药研究与与实践,2017,31(5):48-51.
[11]郝郝晶晶,李海亮,龚龚慕辛.挥发油环糊精包合技术的研究进展及存在在问题分分析[J].中国实验验方剂学杂志,2013,19(2):352-3356.
[12]王王晓琴,曹礼,安玲玲,等.当归和党参参混合挥发油提取取和β-环糊精包包合工艺艺研究[J].中国酿酿造,2017,36(9):153-157.