苯醚甲环唑与戊唑醇混配对水稻稻曲病菌联合作用的测定
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摘要
为明确苯醚甲环唑与戊唑醇不同比例混配对水稻稻曲病菌联合作用的类型,采用病菌菌丝生长抑制率和菌丝生长速率法测定了苯醚甲环唑、戊唑醇及其5种配比对水稻稻曲病菌的毒力作用。结果表明,苯醚甲环唑与戊唑醇对水稻稻曲病菌的EC50分别为1.568、21.487 mg/L,苯醚甲环唑对水稻稻曲病菌的室内生物活性明显优于戊唑醇。在苯醚甲环唑与戊唑醇的5个不同配比中,4:5配比的EC50最小,为1.858 mg/L。1:8、2:7、3:6、4:5混配组合的SR均大于1.5,表明上述4种混配组合对水稻稻曲病菌均表现为增效作用;(2~4):(7~5)配比的SR均大于2.0,增效作用最强。苯醚甲环唑、戊唑醇对水稻稻曲病菌均有较高的室内生物活性,二者按(2~4):(7~5)的比例混配对水稻稻曲病菌有增效作用。建议用药浓度为117~375 mg/L。
Different proportion of difenoconazole and tebuconazole mixed together can affect ustilaginoideavirens. In order to clarify different types of this combined effect, the inhibitory rate of mycelium growth of thepathogen and mycelium growth rate method are used to determine virulence to ustilaginoidea virens fromdifenoconazole, tebuconazole and their 5 matching ratios. The results indicated that EC50 of ustilaginoideavirens was 1.568 and 21.487 mg/L, respectively, when affected by difenoconazole and tebuconazole, and indoorbioactive of ustilaginoidea virens influenced by difenoconazole was more than that influenced by tebuconazole.In the 5 different matching ratios of difenoconazole and tebuconazole, ratio 4:5 had the least EC50, which was1.858 mg/L. Ratio 1:8, 2:7, 3:6 and 4:5 had SR which were all above 1.5 and this illustrated these 4 matchingcombinations all had a synergistic effect towards ustilaginoidea virens. In addition, ratio(2-4):(7-5) produced SR greater than 2.0 and had the strongest synergistic effect. Difenoconazole and tebuconazole both had higherindoor bioactivity to ustilaginoidea virens, and the two with matching ratio of(2-4):(7-5) had the bestsynergistic effect towards ustilaginoidea virens. The suggested drug concentration was 117-375 mg/L.
Different proportion of difenoconazole and tebuconazole mixed together can affect ustilaginoideavirens. In order to clarify different types of this combined effect, the inhibitory rate of mycelium growth of thepathogen and mycelium growth rate method are used to determine virulence to ustilaginoidea virens fromdifenoconazole, tebuconazole and their 5 matching ratios. The results indicated that EC50 of ustilaginoideavirens was 1.568 and 21.487 mg/L, respectively, when affected by difenoconazole and tebuconazole, and indoorbioactive of ustilaginoidea virens influenced by difenoconazole was more than that influenced by tebuconazole.In the 5 different matching ratios of difenoconazole and tebuconazole, ratio 4:5 had the least EC50, which was1.858 mg/L. Ratio 1:8, 2:7, 3:6 and 4:5 had SR which were all above 1.5 and this illustrated these 4 matchingcombinations all had a synergistic effect towards ustilaginoidea virens. In addition, ratio(2-4):(7-5) produced SR greater than 2.0 and had the strongest synergistic effect. Difenoconazole and tebuconazole both had higherindoor bioactivity to ustilaginoidea virens, and the two with matching ratio of(2-4):(7-5) had the bestsynergistic effect towards ustilaginoidea virens. The suggested drug concentration was 117-375 mg/L.
引文
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[11]耿忠义,赵京岚,任永攀,等.氰烯菌酯与己唑醇及其混配对小麦赤霉病菌的影响[J].农学学报,2011(10):29-32.
[12]李喜书,王莎菲,韩新才,等.氯胺磷与井冈霉素混配增效作用研究[J].植物保护,2009,35(2):155-157.
[13]梁伟伶,台莲梅,靳学慧,等.马铃薯早疫病菌室内杀菌剂筛选及配比试验[J].植物保护,2009,35(4):168-171.
[14]冯雪玲.苯醚甲环唑在水稻中的农药残留分析研究[D].北京:北京化工大学,2009.
[15]丁孟,杨仁斌,王海萍,等.ECD气相色谱法对苯醚甲·丙环唑乳油在水稻上的残留研究[J].中国农学通报,2010,26(16):84-89.
[16]张志勇,王冬兰,张存政,等.苯醚甲环唑在水稻和稻田中的残留.中国水稻科学,2011,25(3):339-342.
[17]毕秋艳,马志强,张小风,等.多菌/戊唑醇复配对小麦赤霉病菌抗药性菌株的活性增效作用[J].植物保护,2010,36(2):119-122.
[2]阮宏椿,杨秀娟,石妞妞,等.不同杀菌剂对水稻稻曲病菌的室内毒力及田间药效[J].福建农业学报,2013,28(6):580-583.
[3]杨秀娟,王舒婷,甘林,等.稻曲病菌生长及其分生孢子萌发特性[J].福建农林大学学报,2011,40(6):576-577.
[4]耿忠义,赵京岚,任永攀,等.氰烯菌酯与己唑醇及其混配对小麦赤霉病菌的影响[J].农学学报,2011(10):29-32.
[5]李竞生,王学贵,代海霞,等.几种杀菌剂对水稻稻曲病的防治研究[J].现代农药,2008(3):52-54.
[6]何海永,谭清群,陈小均,等.不同杀菌剂对水稻稻曲病的防治效果评价[J].安徽农学通报,2013(17):1-2.
[7]李威,粟俊,范刚强,等.3种药剂防治水稻纹枯病、稻曲病和稻瘟病田间药效试验[J].生物灾害科学,2013,36(2):174-177.
[8]农业部农药检定所.NY/T 1156.6—2006农药室内生物测定试验准则杀菌剂第6部分:混配的联合作用测定[S].北京:中国农业出版社,2006:1-2.
[9]宗兆锋,康振生.植物病理学原理[M].北京:中国农业出版社,2002:244-245.
[10]黄彰欣.植物化学保护实验指导[M].北京:中国农业出版社,2007:56-58.
[11]耿忠义,赵京岚,任永攀,等.氰烯菌酯与己唑醇及其混配对小麦赤霉病菌的影响[J].农学学报,2011(10):29-32.
[12]李喜书,王莎菲,韩新才,等.氯胺磷与井冈霉素混配增效作用研究[J].植物保护,2009,35(2):155-157.
[13]梁伟伶,台莲梅,靳学慧,等.马铃薯早疫病菌室内杀菌剂筛选及配比试验[J].植物保护,2009,35(4):168-171.
[14]冯雪玲.苯醚甲环唑在水稻中的农药残留分析研究[D].北京:北京化工大学,2009.
[15]丁孟,杨仁斌,王海萍,等.ECD气相色谱法对苯醚甲·丙环唑乳油在水稻上的残留研究[J].中国农学通报,2010,26(16):84-89.
[16]张志勇,王冬兰,张存政,等.苯醚甲环唑在水稻和稻田中的残留.中国水稻科学,2011,25(3):339-342.
[17]毕秋艳,马志强,张小风,等.多菌/戊唑醇复配对小麦赤霉病菌抗药性菌株的活性增效作用[J].植物保护,2010,36(2):119-122.