金针菇子实体膳食纤维对4种肠道菌群体外生长的影响
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摘要
以金针菇子实体为试验材料,研究其可溶性膳食纤维(SDF)与不溶性膳食纤维(IDF)对大肠杆菌、金黄色葡萄球菌、白色假丝酵母及保加利亚乳杆菌体外生长的影响。结果表明,金针菇可溶性膳食纤维对大肠杆菌、金黄色葡萄球菌、白色假丝酵母有显著的抑制作用,而对保加利亚乳杆菌有显著的促进作用,且其抑制或促进效果随着SDF添加量的增加而提高;金针菇不溶性膳食纤维对保加利亚乳杆菌有显著的促生长作用,与其添加量呈正相关,而对大肠杆菌、金黄色葡萄球菌的影响与其添加量有关。添加量为2.4 g/100 m L时,IDF对大肠杆菌和白色假丝酵母的抑制效果最佳,抑制率分别为6.68%和14.49%;添加量为0.6 g/100 m L时,IDF对金黄色葡萄球菌的抑制效果最佳,抑制率为3.50%。该研究结果将会为菌质膳食纤维保健食品的开发提供重要参考依据。
To evaluate the effect of Flammulina velutipes dietary fiber on the growth of intestinal microbiota, soluble dietary fiber(SDF)and insoluble dietary fiber(IDF)from F. velutipes fruit-bodies were used in in vitro cultures of Eschericia coli, Staphylococcus aureus, Monilia albican and Lactobacillus bulgaricus. The results showed that SDF had significant growth-inhibiting effects on E. coli,S. aureus and M. albican, and a significant growth-promoting effect on L. bulgaricus. And its inhibition or promotion effect increased with the increase of SDF content. IDF had significant growth-promoting effect on L. bulgaricus, and was positively correlated with its concentration. The effect of IDF on E. coli, S. aureus were related to the added concentration. IDF had significant inhibitory effects on E.coli and M. albican at the concentration of 2.4 g/100 m L, inhibition rates were 6.68% and 14.49%, respectively. IDF had a significant inhibitory effect on S. aureus at the concentration of 0.6 g/100 m L, its inhibition rate was 3.50%. Furthermore, this study might provide valuable evidences to product health food by using fungal substance dietary fiber in practice.
To evaluate the effect of Flammulina velutipes dietary fiber on the growth of intestinal microbiota, soluble dietary fiber(SDF)and insoluble dietary fiber(IDF)from F. velutipes fruit-bodies were used in in vitro cultures of Eschericia coli, Staphylococcus aureus, Monilia albican and Lactobacillus bulgaricus. The results showed that SDF had significant growth-inhibiting effects on E. coli,S. aureus and M. albican, and a significant growth-promoting effect on L. bulgaricus. And its inhibition or promotion effect increased with the increase of SDF content. IDF had significant growth-promoting effect on L. bulgaricus, and was positively correlated with its concentration. The effect of IDF on E. coli, S. aureus were related to the added concentration. IDF had significant inhibitory effects on E.coli and M. albican at the concentration of 2.4 g/100 m L, inhibition rates were 6.68% and 14.49%, respectively. IDF had a significant inhibitory effect on S. aureus at the concentration of 0.6 g/100 m L, its inhibition rate was 3.50%. Furthermore, this study might provide valuable evidences to product health food by using fungal substance dietary fiber in practice.
引文
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[13]曾巧莉,宋慧,郭慧慧,等.金针菇菇脚对肉鸡盲肠菌群及短链脂肪酸的影响[J].中国农业大学学报,2016,21(5):104-114.
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[16]陈龙,郭晓晖,李富华,等.食用菌膳食纤维功能特性及其应用研究进展[J].食品科学,2012,33(11):303-307.
[2]XU X,XU P,MA C,et al.Gut microbiota,hos thealth,and polysaccharides[J].Biotechnology Advances,2013,31(2):318-337.
[3]扈晓杰,韩冬,李铎.膳食纤维的定义、分析方法和摄入现状[J].中国食品学报,2011,6(3):133-137.
[4]陈燕卉,陈敏,张绍英,等.膳食纤维在食品加工中的应用与研究进展[J].食品科学,2004,25(Z1):253-257.
[5]鄢嫣,陈洁.食用菌深加工的现状及产业前景[N].中国食品报,2012-10-05.
[6]SHUKLA R,CHERYAN M.Zein:the industrial protein from corn[J].Industrial Crops and Products,2001,13(3):171-192.
[7]刘勇男,常明昌,刘靖宇,等.刺芹侧耳子实体中水溶性膳食纤维的提取工艺研究[J].食用菌学报,2014(4):67-70.
[8]刘靖宇,阎伟,孟俊龙,等.刺芹侧耳子实体水不溶性膳食纤维的提取工艺研究[J].山西农业大学学报(自然科学版),2014(2):189-192.
[9]沈萍,范秀容,李广武.微生物学实验[M].3版.北京:高等教育出版社,1999:49-100.
[10]彭章普,龚伟中,徐艳,等.苹果渣可溶性膳食纤维提取工艺的研究[J].食品科技,2007(7):238-241.
[11]韩冬.膳食纤维与肠道健康[J].中国微生态学杂志,2013,25(10):1225.
[12]余有贵,李忠海,黄国华,等.菌质可溶性膳食纤维对肠道菌群体外生长的影响[J].中国食品学报,2015,10(2):34-38.
[13]曾巧莉,宋慧,郭慧慧,等.金针菇菇脚对肉鸡盲肠菌群及短链脂肪酸的影响[J].中国农业大学学报,2016,21(5):104-114.
[14]孙元琳,陕方,赵立平.谷物膳食纤维:戊聚糖与肠道菌群调节研究进展[J].食品科学,2012,33(9):326-330.
[15]吴占威,胡志和,邬雄志.豆渣膳食纤维及豆渣超微化制品对小鼠肠道菌群的影响[J].食品科学,2013,34(3):271-275.
[16]陈龙,郭晓晖,李富华,等.食用菌膳食纤维功能特性及其应用研究进展[J].食品科学,2012,33(11):303-307.