34份玉米种质材料的亲缘关系分析
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摘要
使用56K的基因芯片对34份玉米种质资源进行基因组水平上的分子鉴定。34份玉米种质材料在遗传结构上可以分为籽粒玉米种质和青贮玉米种质两种类型,大部分未知种质的遗传结构中青贮种质的遗传比例相对较大。通过亲缘关系分析发现种质材料亲缘关系较远的前5个种质材料分别为27(-5.000 64)>2(-3.300 79)>25(-1.475 92)>18(-1.352 37)>15(-1.217 94)。有20对种质材料间存在较大的亲缘关系,其亲缘关系系数在0.2以上,25对种质材料间存在一定的亲缘关系,其亲缘关系系数在0.1~0.2。大部分未知青贮种质在遗传结构上具有较大的青贮种质遗传比例,是潜在的青贮玉米种质创新材料;种质材料27、2、25、18、15的亲缘关系较远。
The research for breeding silage maize is limited in China due to the lack of silage maize germplasms,unclear genetic structure and kinship relationship of silage maize germplasms,therefore maize germplasms research genetic structure and kinship relationship were discovered to improve breeding criteria. Single nucleotide polymorphism of 34 maize germplasm resources was performed using a 56 K gene chip. The genetic structure of 34 maize germplasms could be divided into grain maize and silage maize types,unclear maize germplasms had high proportion of silage genetic structure.The lowest kinship coefficient of 5 maize germplasms in our research were maize germplasm 27(-5. 000 64) > maize germplasm 2(-3. 300 79) > maize germplasm 25(-1. 475 92) > maize germplasm 18(-1. 352 37) > maize germplasm 15(-1. 217 94). Over 0. 2 kinship coefficient were identified by 20 maize germplasms and kinship coefficient at 0. 1 to 0. 2 were identified by 25 maize germplasms. Most of unclear maize germplasms that were adopted to silage maize breeding had high proportion of silage genetic structure. Maize germplasm 27,2,25,18,15 that were useful silage maize lines had far kinship relationship.
The research for breeding silage maize is limited in China due to the lack of silage maize germplasms,unclear genetic structure and kinship relationship of silage maize germplasms,therefore maize germplasms research genetic structure and kinship relationship were discovered to improve breeding criteria. Single nucleotide polymorphism of 34 maize germplasm resources was performed using a 56 K gene chip. The genetic structure of 34 maize germplasms could be divided into grain maize and silage maize types,unclear maize germplasms had high proportion of silage genetic structure.The lowest kinship coefficient of 5 maize germplasms in our research were maize germplasm 27(-5. 000 64) > maize germplasm 2(-3. 300 79) > maize germplasm 25(-1. 475 92) > maize germplasm 18(-1. 352 37) > maize germplasm 15(-1. 217 94). Over 0. 2 kinship coefficient were identified by 20 maize germplasms and kinship coefficient at 0. 1 to 0. 2 were identified by 25 maize germplasms. Most of unclear maize germplasms that were adopted to silage maize breeding had high proportion of silage genetic structure. Maize germplasm 27,2,25,18,15 that were useful silage maize lines had far kinship relationship.
引文
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[4]孟令聪,路明,张志军,等.我国青贮玉米育种研究进展[J].北方农业学报,2016,44(4):99-104.
[5]张先文,贺治洲,江南,等.高通量基因型分型技术及其在水稻中的应用[J].生物技术通报,2017,33(12):1-6.
[6]邹喻苹,葛颂.新一代分子标记——SNPs及其应用[J].生物多样性,2003,11(5):370-380(特刊).
[7]王阳,候佳明,李继洪,等.基于SNP芯片的玉米黄改系遗传基础研究[J].东北农业科学,2016,41(4):5-7.
[8]路明,张志军,郑淑波,等.基于SNP标记分析玉米品种吉单50的遗传基础[J].玉米科学,2016,24(6):1-7.
[9]史亚兴,卢柏山,宋伟,等.基于SNP标记技术的糯玉米种质遗传多样性分析[J].华北农学报,2015,30(3):77-82.
[10]廖俊杰.采用CTAB法快速提取植物DNA[J].天津农业科学,1993(3):26.
[11]Alexander D H,Novembre J,Lange K.Fast model-based estimation of ancestry in unrelated individuals[J].Genome Research,2009,19:1655-1664.
[12]Manichaikul A,Palmas W,Rodriguez CJ,et al.Population structure of Hispanics in the United States:the multi-ethnic study of atherosclerosis[J].Plos Genetics,2012,8(4):1-14.
[13]王文斌,徐淑兔,高杰,等.基于SNP标记的玉米自交系遗传多样性分析[J].玉米科学,2015,23(2):41-45.
[14]吴承来,张倩倩,董炳雪,等.我国部分玉米自交系遗传关系和遗传结构解析[J].作物学报,2010,36(11):1820-1831.
[15]赵旭,方永丰,王汉宁.玉米SSR标记杂优类群划分及群体遗传结构分析[J].核农学报,2013,27(12):1828-1838.
[16]赵文明,陈艳萍,孟庆长,等.32份糯玉米自交系的遗传多样性分析[J].江西农业学报,2012,24(12):6-8.
[17]夏春兰,曹聪,江浩,等.新疆玉米自交系群体结构和聚类分析[J].分子植物育种,2017,15(3):1061-1068.