植物源抑菌物质的筛选及其应用
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摘要
本试验主要研究了植物源抗菌物质特性,筛选可替代抗生素的原料。利用药敏试验(标准菌株大肠杆菌各型、金黄色葡萄球菌、沙门氏菌及霉菌)及临床攻毒试验对12种常见中草药进行研究分析。结果表明,12种单味中草药的粗提物中黄连、夏枯草、黄芩、连翘、石榴皮对大肠杆菌均有抑制作用,且效果明显强于其他提取液;临床试验结果表明黄芩、夏枯草、连翘和金银花对鸡的攻毒保护率高于感染对照组5%~10%。
To study the properties of antibacterial substances obtained from plant source,screening raw materials tosubstitute for antibiotic. By drug sensitive test(various types of standard strains of Escherichia coli, Staphylococcus aureus, Salmonella and mold) and Clinical challenge tests on 12 common herbs for research and analysis. Conclusions: E. coli was inhibited by12 kinds of single herb herbal crude extracts of Goldthread, Prunella, Scutellaria, Forsythia,pomegranate rind, and the effect was stronger than the other extracts. Clinical trials showed that radix scutellariae, selfheal, flos lonicerae, fructus forsythiae and tapping poison protection ratio is 5%~10% higher than the control group.
To study the properties of antibacterial substances obtained from plant source,screening raw materials tosubstitute for antibiotic. By drug sensitive test(various types of standard strains of Escherichia coli, Staphylococcus aureus, Salmonella and mold) and Clinical challenge tests on 12 common herbs for research and analysis. Conclusions: E. coli was inhibited by12 kinds of single herb herbal crude extracts of Goldthread, Prunella, Scutellaria, Forsythia,pomegranate rind, and the effect was stronger than the other extracts. Clinical trials showed that radix scutellariae, selfheal, flos lonicerae, fructus forsythiae and tapping poison protection ratio is 5%~10% higher than the control group.
引文
[1]崔一喆,张秀英,王秋菊,等.中草药对奶牛乳房炎致病菌体外抑菌筛选[J].中兽医医药杂志,2006,25(3):35-36.
[2]温周瑞,刘慧敏,骆敏,等.中草药对水产动物免疫作用研究进展[J].水利渔业,2004,24(2):1-3.
[3]吴增华,徐铁山,朱明,等.中草药添加剂对断奶羔羊增重及免疫力的影响[J].现代畜牧兽医,2012,12:49-50.
[4]温晓雪,王林.植物源性抗菌药物的前景[J].国外医学(药学分册),2007,34(3):233-234.
[5]万遂如.中兽药制剂在猪病防控中的科学应用[J].现代畜牧兽医,2014,1:27-33.
[6]林洋,曹艳子,谭娜,等.不同来源抗菌肽对人工感染鸡大肠杆菌的试验研究[J].黑龙江畜牧兽医,2014,1(上):150-151.
[7]刘骏,邓菲,李柯静,等.中药桔梗及其临床配伍的研究进展[J].中国药业,2014,23(8):84-86.
[8]王雪.大黄的抑菌作用分析[J].中国科技博览,2014,(4):250.
[9]王红刚,潘秋婷,梁斯婷.千草根粗提取物体外抑菌活性部位的筛选研究[J].海峡药学,2014,26(2):147-149.
[10]杜庆波.黄芩药理活性研究新进展[J].齐齐哈尔医学院学报,2014,(1):110-111.
[11]问鑫,高凤仙.植物提取物的作用机理及在猪生产中的应用[J].中国饲料,2013,(11):1-4.
[12]甘利平(译),张克富(译),张桂国(译).草本植物及其提取物作为生长促进剂在动物营养中的应用研究进展[J].中国饲料添加剂,2012,(9):31-35.
[2]温周瑞,刘慧敏,骆敏,等.中草药对水产动物免疫作用研究进展[J].水利渔业,2004,24(2):1-3.
[3]吴增华,徐铁山,朱明,等.中草药添加剂对断奶羔羊增重及免疫力的影响[J].现代畜牧兽医,2012,12:49-50.
[4]温晓雪,王林.植物源性抗菌药物的前景[J].国外医学(药学分册),2007,34(3):233-234.
[5]万遂如.中兽药制剂在猪病防控中的科学应用[J].现代畜牧兽医,2014,1:27-33.
[6]林洋,曹艳子,谭娜,等.不同来源抗菌肽对人工感染鸡大肠杆菌的试验研究[J].黑龙江畜牧兽医,2014,1(上):150-151.
[7]刘骏,邓菲,李柯静,等.中药桔梗及其临床配伍的研究进展[J].中国药业,2014,23(8):84-86.
[8]王雪.大黄的抑菌作用分析[J].中国科技博览,2014,(4):250.
[9]王红刚,潘秋婷,梁斯婷.千草根粗提取物体外抑菌活性部位的筛选研究[J].海峡药学,2014,26(2):147-149.
[10]杜庆波.黄芩药理活性研究新进展[J].齐齐哈尔医学院学报,2014,(1):110-111.
[11]问鑫,高凤仙.植物提取物的作用机理及在猪生产中的应用[J].中国饲料,2013,(11):1-4.
[12]甘利平(译),张克富(译),张桂国(译).草本植物及其提取物作为生长促进剂在动物营养中的应用研究进展[J].中国饲料添加剂,2012,(9):31-35.