温度和光照对浮游植物叶绿素a提取的影响
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摘要
采用分光光度法测定浮游植物中叶绿素a的含量,研究光照和温度对提取浮游植物叶绿素a的影响,以确定提取浮游植物叶绿素a的最佳条件。在抽滤过程中严格避光,并用冰水混合物冷却至0℃保存,抽滤水样。浸提分为避光(低于200 lx)冷藏(-1~1℃)组、光照(1 100~1 700l x)冷藏(-1~1℃)组、避光(低于2 0 0 l x)常温(2 6~3 0℃)组和光照(1 100~1 700 lx)常温(26~30℃)组,每组放入10 m L丙酮溶液(90%)浸提19 h。4组平均提取量分别为:避光冷藏组155.73μg/L;光照冷藏组136.24μg/L;避光常温组116.67μg/L;光照常温组89.85μg/L。结果表明浮游植物叶绿素a的最佳提取条件为冷藏、避光。
The spectrophotometric method in different conditions of light and temperature, extraction of phytoplankton chlorophyll a determined, to study the different illumination and temperature on the extraction of phytoplankton chlorophyll a and determine phytoplankton chlorophyll a in the optimum conditions of extraction. Strictly avoid light in the filtration process, and use the ice water mixture is cooled to 0 ℃ preservation, water filtration. Leaching provided to avoid light(less than 200 lx refrigerated(-1-1 ℃)group, light(1 100-1 700 lx)cold(-1-1 ℃)group, evades the light(less than 200 lx)at room temperature(26-30 ℃)group and light(1 100-1 700 lx)at room temperature(26-30 ℃)group, each group into the 90% acetone solution extraction 19 h. The average extraction amounts of the four groups were as follows: 155.73 μg/L of the cold storage group and 136.24 μg/L in the light cold storage group, 116.67 μg/L in the ambient temperature group and 89.85 μg/L in the normal temperature group. The results showed that the optimal extraction conditions of phytoplankton chlorophyll a were cold storage and light avoidance.
The spectrophotometric method in different conditions of light and temperature, extraction of phytoplankton chlorophyll a determined, to study the different illumination and temperature on the extraction of phytoplankton chlorophyll a and determine phytoplankton chlorophyll a in the optimum conditions of extraction. Strictly avoid light in the filtration process, and use the ice water mixture is cooled to 0 ℃ preservation, water filtration. Leaching provided to avoid light(less than 200 lx refrigerated(-1-1 ℃)group, light(1 100-1 700 lx)cold(-1-1 ℃)group, evades the light(less than 200 lx)at room temperature(26-30 ℃)group and light(1 100-1 700 lx)at room temperature(26-30 ℃)group, each group into the 90% acetone solution extraction 19 h. The average extraction amounts of the four groups were as follows: 155.73 μg/L of the cold storage group and 136.24 μg/L in the light cold storage group, 116.67 μg/L in the ambient temperature group and 89.85 μg/L in the normal temperature group. The results showed that the optimal extraction conditions of phytoplankton chlorophyll a were cold storage and light avoidance.
引文
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[2]Yentsch C S,Menzel D W.A method or the determination of phytoplankton chlorophyll and phaeophytin by fluorescene[J].Deep Sea Research,1963,10:221-231.
[3]Schmid H,Bauer F,Stich H.Determination of algal biomass with HPLC pigment analysis from lakes of different trophic state in comparison to microscopically measured biomass[J].Journal of Plankton Research,1998,20:1651-1661.
[4]国家标准化管理委员会.海洋监测规范:第7部分:GB17378.7—2007[S].北京:中国标准出版社,2008.
[5]张武昌,王荣.光和温度对叶绿素a和脱镁叶绿酸a降解的影响[J].海洋科学,2000,2(4):50-52.
[6]刘丽雪,王玉珏,邸宝平,等.2012年春季渤海中部及邻近海域叶绿素a与环境因子的分布特征[J].海洋科学,2014,38(12):8-15.
[7]郑晓艳,郭隽.浅谈地表水叶绿素a含量的测定[C]//中国环境科学学会.中国环境科学学会学术年会论文集(第四卷):2013年卷.北京:中国环境科学出版社,2013.
[8]黄昌妙,叶树才,王潮.浮游植物叶绿素a测定方法的比较分析[J].福建分析测试,2013,22(4):23-27.
[9]林少君,贺立静,黄沛生,等.浮游植物中叶绿素a提取方法的比较与改进[J].生态科学,2005,24(1):9-11.
[10]徐彩平,刘霞,陈宇炜.浮游植物叶绿素a浓度测定方法的比较研究[J].生态与农村环境学报,2013,29(4):438-442.
[11]周静,胡忠军,李培培,等.基于叶绿素a测定的分光光度法与bbe法比较:以千岛湖为例[J].水生态学杂志,2013,34(2):80-84.