ZnS量子点荧光探针对Pb~(2+)的检测研究
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摘要
以L-半胱氨酸修饰ZnS量子点为荧光探针,测定火腿肠中Pb~(2+)含量。对ZnS量子点进行了UV、IR、荧光性能表征。研究了酸度、ZnS量子点浓度对体系荧光强度的影响,最佳测定条件为pH 8,L-半胱氨酸-ZnS量子点(L-ZnS量子点)浓度为1.0×10~(-6)mol/L。基于Pb~(2+)浓度对体系的荧光猝灭效应,实现对Pb~(2+)的定量检测,Pb~(2+)浓度在1.0×10~(-7)~4.5×10~(-5)mol/L范围内与体系的荧光强度线性关系良好,R2=0.984 7,检出限(3σ/S)为3.7×10~(-8)mol/L。线性方程为F0/F=88.14-14.05cPb~(2+),RSD=5.4%(n=6)。该方法简便、灵敏度高,可应用到检测Pb~(2+)诸多方面。
Determination of Pb~(2+) ion content in ham was conducted by a fluorescent probe made of L-cysteine modified ZnS quantum dots.ZnS quantum dots were investigated by ultraviolet visible absorption(UV),infrared radiation(IR),fluorescence spectrum(FS).The effects of the system acidity and the concentration of ZnS quantum dots on the fluorescence intensity were investigated.Optimum condition was obtained as pH 8.0 and cL-ZnS= 1.0×10~(-6) mol/L.Quantitative detection of Pb~(2+) was achieved according to the quenched luminescence extent of ZnS quantum dots by Pb~(2+).The fluorescence intensity of the whole system shows a good linear relationship with the concentration of Pb~(2+)in the range of 1.0×10~(-7)~4.5×10~(-5) mol/L.The linear regression equation can be described F0/F=88.14-14.05 cPb~(2+),and the correlation coefficient is calculated to be 0.984 7.The limit detection is 3.7×10-8 mol/L and RSD=5.4%(n=6).The method is simple and sensitive,which can be applied for the determination of Pb~(2+) concentration in many actual systems.
Determination of Pb~(2+) ion content in ham was conducted by a fluorescent probe made of L-cysteine modified ZnS quantum dots.ZnS quantum dots were investigated by ultraviolet visible absorption(UV),infrared radiation(IR),fluorescence spectrum(FS).The effects of the system acidity and the concentration of ZnS quantum dots on the fluorescence intensity were investigated.Optimum condition was obtained as pH 8.0 and cL-ZnS= 1.0×10~(-6) mol/L.Quantitative detection of Pb~(2+) was achieved according to the quenched luminescence extent of ZnS quantum dots by Pb~(2+).The fluorescence intensity of the whole system shows a good linear relationship with the concentration of Pb~(2+)in the range of 1.0×10~(-7)~4.5×10~(-5) mol/L.The linear regression equation can be described F0/F=88.14-14.05 cPb~(2+),and the correlation coefficient is calculated to be 0.984 7.The limit detection is 3.7×10-8 mol/L and RSD=5.4%(n=6).The method is simple and sensitive,which can be applied for the determination of Pb~(2+) concentration in many actual systems.
引文
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[3]杨志广,王筠,段朋云.有机p H荧光探针研究进展[J].化学试剂,2015,37(1):44-48.
[4]张秀清,彭君,刘朝娟,等.基于银纳米三角片与罗丹明6G的荧光工作能量转移法检测钴离子[J].光谱学与光谱分析,2015,35(4):955-959.
[5]张艺,李紫薇,高红艳.聚丙烯酸功能化La F3:Ce3+/Tb3+荧光探针测定鲑鱼精DNA[J].应用化工,2015,44(5):956-958.
[6]张昊,刘传志,徐影,等.生物荧光传感器检测环境水样中氨基甲酸酯类农药残留[J].分析化学,2014,42(1):104-108.
[7]赵巍,汪钊,蔡强,等.基于荧光传感器Frex的特性检测水质环境中生物毒性物质[J].环境科学,2014,35(6):2 281-2 287.
[8]谢光杰,户明星,徐丽萍,等.选择性生物小分子硫醇荧光探针的研究进展[J].化学研究与应用,2016,28(9):1 177-1 185.
[9]CHEN Yong-fen,ROSENZWEIG Z.Luminescent Cd S quantum dots as selective ion probes[J].Anal.Chem.,2002,86(12):5 611-5 614.
[10]KRAVTSOVA A N,PANKIN I A,BUDNYK A P,et al.Synthesis and structure modeling of Zn S based quantum dots[J].J.Struct.Chem.,2016,57(5):926-933.
[11]GAYNANOVA G A,VASILIEVA E A,BEKMUKHAMETOVA A M,et al.Encapsulation of quantum dots in supramolecular systems based on amphiphilic compounds and polyelectrolytes[J].Russ.Chem.Bull.,2016,65(1):151-157.
[12]王雪婷,于俊生,谢颖.水溶性Cd Se/Cd S量子点和合成及其与牛血清蛋白的共轭作用[J].无机化学学报,2007,23(7):1 185-1 193.
[13]周磊,殷桃刚,黄玲.水溶性Cd Se量子点的制备[J].当代化工,2011,2(40):121-123.
[14]刘岩,白晓,李婷婷,等.量子点@Si O2微球的水相合成及其在流式分析技术中的应用[J].分析科学学报,2016,32(5):629-633.
[15]王玉珍,祝明,黄海龙,等.PGMA-g-EDA梳状聚合物的制备及其对量子点修饰的研究[J].高分子学报,2013,(8):1 039-1 046.
[16]王妍,贾红霞,曹雨虹,等.基于硫化镉量子点的应该猝灭效应测定微量钴离子[J].理化检验-化学分册,2016,52(10):1 123-1 126.