旱田草的HPLC指纹图谱构建及聚类分析
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摘要
目的采用指纹图谱与系统聚类分析方法评价不同产地旱田草的质量。方法采用Phenomenex C_(18)色谱柱(250 mm×4. 60 mm,5μm),流动相为乙腈-0. 2%磷酸水溶液,梯度洗脱,检测波长334 nm,柱温30℃;建立旱田草药材的HPLC指纹图谱,对14批药材进行相似度评价,并进行聚类分析。结果 14批旱田草药材HLPC指纹图谱的精密度、重复性及稳定性的RSD均<3%,指纹图谱的相似度均> 0. 96; 14批旱田草总共可聚为5类。结论 HPLC指纹图谱与聚类分析相结合,数据稳定可靠,方法简便高效,可为旱田草的质量评价提供参考。
OBJECTIVE To evaluate the quality evaluation of Lindernia ruellioides in different producing areas based on fingerprint and cluster analysis. METHODS The chromatographic column was phenomenex C_(18)( 250 mm × 4. 60 mm,5 μm). Flow phase was acetonitrile-0. 2% phosphoric acid aqueous solution,gradient elution. The detection wavelength was 334 nm and the column temperature was 30 ℃. HPLC fingerprints were established to evaluate the similarity and the clustoring of 14 batches of L. ruellioides.RESULTS The established HPLC fingerprint chromatogram of L. ruellioides was specfic,precise,reproduccible and stable. The similarities of 14 batches of L. ruellioides were more than 0. 96. Further cluster analysis was carried out for 14 batches of L. ruellioides and accordingly they were devided into 5 clusters. CONCLUSION Combining HPLC fingerprint and cluster analysis,the data is stable and reliable,and the method is simple and efficient,which can provide a reference for the quality evaluation of L. ruellioides.
OBJECTIVE To evaluate the quality evaluation of Lindernia ruellioides in different producing areas based on fingerprint and cluster analysis. METHODS The chromatographic column was phenomenex C_(18)( 250 mm × 4. 60 mm,5 μm). Flow phase was acetonitrile-0. 2% phosphoric acid aqueous solution,gradient elution. The detection wavelength was 334 nm and the column temperature was 30 ℃. HPLC fingerprints were established to evaluate the similarity and the clustoring of 14 batches of L. ruellioides.RESULTS The established HPLC fingerprint chromatogram of L. ruellioides was specfic,precise,reproduccible and stable. The similarities of 14 batches of L. ruellioides were more than 0. 96. Further cluster analysis was carried out for 14 batches of L. ruellioides and accordingly they were devided into 5 clusters. CONCLUSION Combining HPLC fingerprint and cluster analysis,the data is stable and reliable,and the method is simple and efficient,which can provide a reference for the quality evaluation of L. ruellioides.
引文
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[2]覃海宁,刘演.广西植物名录[M].北京:科学出版社,2010:378-379.
[3]梁启成,钟鸣.中国壮药学[M].南宁:广西民族出版社,2005:397.
[4]唐玉荣,颜萍花,曾祥燕,等.旱田草显微鉴别研究[J].时珍国医国药,2015,26(8):1938-1939.
[5]唐玉荣,颜萍花,曾祥燕,等.旱田草的生药学研究[J].华西药学杂志,2016,31(4):386-388.
[6]曹雨虹,武尉杰,谭睿,等. HPLC测定母草属药用植物中的齐墩果酸和绿原酸[J].华西药学杂志,2015,30(4):514-516.