甘蓝型油菜隐性细胞核雄性不育的研究及利用
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摘要
甘蓝型油菜隐性细胞核雄性不育系育性稳定、无负效应、易转育且恢复源广,在油菜杂种优势利用中被广泛利用.目前我国主要利用的隐性细胞核雄性不育系有2类:双基因隐性细胞核雄性不育以及由2对基因互作控制的核不育类型.随着这2类不育系不育基因相继被克隆,人们对甘蓝型油菜细胞核雄性不育的分子作用机理已经有一定了解.本文综述了在甘蓝型油菜核不育分子机理的研究方面的进展,在此基础上提出了繁殖甘蓝型油菜核不育系全不育系群体可行的途径,展现了油菜细胞核雄性不育杂种优势利用广阔的前景.
The Brassica napus recessive genic male sterile(RGMS) line was widely used in heterosis utilization because it possesses stable sterility, no negative cytoplasmic effects, easy restoration and multi-restored resources. There are mainly two recessive genic male sterile lines in China. One is controlled by two duplicate recessive genes, and another is controlled by two loci, one recessive mutant locus and one multiple allele locus. With cloning of these sterile genes, some molecular mechanisms of genic male sterility in Brassica napus was deciphered. In this review, we summarize the advances in research on molecular mechanism of genic male sterility in Brassica napus. A feasible way is proposed to breed entire sterile populations of genic male sterile line in Brassica napus, which shows broad prospects in heterosis utilization.
The Brassica napus recessive genic male sterile(RGMS) line was widely used in heterosis utilization because it possesses stable sterility, no negative cytoplasmic effects, easy restoration and multi-restored resources. There are mainly two recessive genic male sterile lines in China. One is controlled by two duplicate recessive genes, and another is controlled by two loci, one recessive mutant locus and one multiple allele locus. With cloning of these sterile genes, some molecular mechanisms of genic male sterility in Brassica napus was deciphered. In this review, we summarize the advances in research on molecular mechanism of genic male sterility in Brassica napus. A feasible way is proposed to breed entire sterile populations of genic male sterile line in Brassica napus, which shows broad prospects in heterosis utilization.
引文
1 潘涛,曾凡亚,吴书慧,等.甘蓝型低芥酸油菜雄性不育两用系的选育与利用研究.中国油料,1988,3:5–8
2 侯国佐,王华,张瑞茂.甘蓝型油菜细胞核雄性不育材料117A的遗传研究.中国油料作物学报,1990,12:7–10
3 陈凤祥,胡宝成,李成,等.甘蓝型油菜细胞核雄性不育性的遗传研究Ⅰ.隐性核不育系9012A的遗传.作物学报,1998,24:431 –438
4 Huang Z,Chen Y,Yi B,et al.Fine mapping of the recessive genic male sterility gene(Bnms3)in Brassica napus L.Theor Appl Genet,2007,115:113–118
5 傅廷栋.杂交油菜的育种与利用.武汉:湖北科学技术出版社,1995
6 陈玉宁.甘蓝型油菜S45AB隐性细胞核雄性不育表达谱的研究.博士学位论文.武汉:华中农业大学,2009
7 曾芳琴.油菜S45AB隐性核不育分子机理与应用研究.博士学位论文.武汉:华中农业大学,2011
8 涂金星,傅廷栋,郑用琏.甘蓝型油菜核不育材料育性基因的RAPD标记.华中农业大学学报,1997,16:112–117
9 Yi B,Chen Y N,Lei S L,et al.Fine mapping of the recessive genic male-sterile gene(Bnms1)in Brassica napus L.Theor Appl Genet,2006,4:643–650
10 Lei S,Yao X,Yi B,et al.Towards map-based cloning:fine mapping of a recessive genic male-sterile gene(BnMs2)in Brassica napus L.and syntenic region identification based on the Arabidopsis thaliana genome sequences.Theor Appl Genet,2007,115:643–651
11 Yi B,Zeng F Q,Lei S L,et al.Two duplicate CYP704B1-homologous genes BnMs1 and BnMs2 are required for pollen exine formation and tapetal development in Brassica napus.Plant J,2010,6:925–938
12 蒙大庆,袁代斌,郭子荣,等.双低甘蓝型油菜新型核不育系绵7AB-4的遗传研究Ⅰ.育性表现的初步研究.四川农业大学学报,2005,23:24–28
13 Xiao L,Yi B,Chen Y F,et al.Molecular markers linked to Bn;rf:a recessive epistatic inhibitor gene of recessive genic male sterility in Brassica napus L.Euphytica,2008,2:377–384
14 陈凤祥,胡宝成,李强生,等.甘蓝型油菜隐性上位互作核不育双低杂交种皖油14号的选育.中国油料作物学报,2003,25:63–65
15 陈凤祥,胡宝成,李强生,等.甘蓝型油菜隐性上位互作核不育双低杂交种“皖油18”的选育.安徽农业科学,2002,30:535–537
16 孙超才,赵华,王伟荣,等.甘蓝型双低隐性核不育杂交种沪油杂1号的选育.中国油料作物学报,2004,26:63–65
17 Zhu Y,Dun X L,Zhou Z F,et al.A separation defect of tapetum cells and microspore mother cells results in male sterility in Brassica napus:the role of abscisic acid in early anther development.Plant Mol Biol,2010,72:111–123
18 Dun X L,Zhou Z F,Xia S Q,et al.BnaC.Tic40,a plastid inner membrane translocon originating from Brassica oleracea,is essential for tapetal function and microspore development in Brassica napus.Plant J,2011,68:532–545
19 Zhou Z F,Dun X L,Xia S Q,et al.BnMs3 is required for tapetal differentiation and degradation,microspore separation,and pollen wall biosynthesis in Brassica napus.J Exp Bot,2012,63:2041–2058
20 俎峰,夏胜前,顿小玲,等.基于分子标记的油菜隐性核不育7-7365AB遗传模式探究.中国农业科学,2010,43:3067–3075
21 Zhu J,Chen H,Li H,et al.Defective in Tapetal development and function 1 is essential for anther development and tapetal function for microspore maturation in Arabidopsis.Plant J,2008,2:266–277
22 Zhu J,Lou Y,Xu X F,et al.A genetic gathway for tapetum development and function in Arabidopsis.J Integr Plant Biol,2011,11:892–900
23 Zhang Z,Zhu J,Gao J,et al.Transcription factor AtMYB103 is required for anther development by regulating tapetum development,callose dissolution and exine formation in Arabidopsis.Plant J,2007,3:528–538
24 Preuss D,Rhee S Y,Davis R W.Tetrad analysis possible in Arabidopsis with mutation of the QUARTET(QRT)genes.Science,1994,5164:1458–1460.
25 Rhee S Y,Somerville C R.Tetrad pollen formation in quartet mutants of Arabidopsis thaliana is associated with persistence of pectic polysaccharides of the pollen mother cell wall.Plant J,1998,15:79–88
2 侯国佐,王华,张瑞茂.甘蓝型油菜细胞核雄性不育材料117A的遗传研究.中国油料作物学报,1990,12:7–10
3 陈凤祥,胡宝成,李成,等.甘蓝型油菜细胞核雄性不育性的遗传研究Ⅰ.隐性核不育系9012A的遗传.作物学报,1998,24:431 –438
4 Huang Z,Chen Y,Yi B,et al.Fine mapping of the recessive genic male sterility gene(Bnms3)in Brassica napus L.Theor Appl Genet,2007,115:113–118
5 傅廷栋.杂交油菜的育种与利用.武汉:湖北科学技术出版社,1995
6 陈玉宁.甘蓝型油菜S45AB隐性细胞核雄性不育表达谱的研究.博士学位论文.武汉:华中农业大学,2009
7 曾芳琴.油菜S45AB隐性核不育分子机理与应用研究.博士学位论文.武汉:华中农业大学,2011
8 涂金星,傅廷栋,郑用琏.甘蓝型油菜核不育材料育性基因的RAPD标记.华中农业大学学报,1997,16:112–117
9 Yi B,Chen Y N,Lei S L,et al.Fine mapping of the recessive genic male-sterile gene(Bnms1)in Brassica napus L.Theor Appl Genet,2006,4:643–650
10 Lei S,Yao X,Yi B,et al.Towards map-based cloning:fine mapping of a recessive genic male-sterile gene(BnMs2)in Brassica napus L.and syntenic region identification based on the Arabidopsis thaliana genome sequences.Theor Appl Genet,2007,115:643–651
11 Yi B,Zeng F Q,Lei S L,et al.Two duplicate CYP704B1-homologous genes BnMs1 and BnMs2 are required for pollen exine formation and tapetal development in Brassica napus.Plant J,2010,6:925–938
12 蒙大庆,袁代斌,郭子荣,等.双低甘蓝型油菜新型核不育系绵7AB-4的遗传研究Ⅰ.育性表现的初步研究.四川农业大学学报,2005,23:24–28
13 Xiao L,Yi B,Chen Y F,et al.Molecular markers linked to Bn;rf:a recessive epistatic inhibitor gene of recessive genic male sterility in Brassica napus L.Euphytica,2008,2:377–384
14 陈凤祥,胡宝成,李强生,等.甘蓝型油菜隐性上位互作核不育双低杂交种皖油14号的选育.中国油料作物学报,2003,25:63–65
15 陈凤祥,胡宝成,李强生,等.甘蓝型油菜隐性上位互作核不育双低杂交种“皖油18”的选育.安徽农业科学,2002,30:535–537
16 孙超才,赵华,王伟荣,等.甘蓝型双低隐性核不育杂交种沪油杂1号的选育.中国油料作物学报,2004,26:63–65
17 Zhu Y,Dun X L,Zhou Z F,et al.A separation defect of tapetum cells and microspore mother cells results in male sterility in Brassica napus:the role of abscisic acid in early anther development.Plant Mol Biol,2010,72:111–123
18 Dun X L,Zhou Z F,Xia S Q,et al.BnaC.Tic40,a plastid inner membrane translocon originating from Brassica oleracea,is essential for tapetal function and microspore development in Brassica napus.Plant J,2011,68:532–545
19 Zhou Z F,Dun X L,Xia S Q,et al.BnMs3 is required for tapetal differentiation and degradation,microspore separation,and pollen wall biosynthesis in Brassica napus.J Exp Bot,2012,63:2041–2058
20 俎峰,夏胜前,顿小玲,等.基于分子标记的油菜隐性核不育7-7365AB遗传模式探究.中国农业科学,2010,43:3067–3075
21 Zhu J,Chen H,Li H,et al.Defective in Tapetal development and function 1 is essential for anther development and tapetal function for microspore maturation in Arabidopsis.Plant J,2008,2:266–277
22 Zhu J,Lou Y,Xu X F,et al.A genetic gathway for tapetum development and function in Arabidopsis.J Integr Plant Biol,2011,11:892–900
23 Zhang Z,Zhu J,Gao J,et al.Transcription factor AtMYB103 is required for anther development by regulating tapetum development,callose dissolution and exine formation in Arabidopsis.Plant J,2007,3:528–538
24 Preuss D,Rhee S Y,Davis R W.Tetrad analysis possible in Arabidopsis with mutation of the QUARTET(QRT)genes.Science,1994,5164:1458–1460.
25 Rhee S Y,Somerville C R.Tetrad pollen formation in quartet mutants of Arabidopsis thaliana is associated with persistence of pectic polysaccharides of the pollen mother cell wall.Plant J,1998,15:79–88