红芝漆酶高产菌株的诱变选育
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摘要
以药用真菌红芝(Ganoderma lucidum)为出发菌,通过紫外线(Ultraviolet ray,UV)、亚硝基胍(Nitrosoguanidine,NTG)及紫外线(UV)-亚硝基胍(NTG)逐级诱变处理其菌丝体片段,采用平板变色法初筛、愈创木酚法测定发酵液漆酶酶活力复筛,获得1株漆酶高产诱变菌株LYL263。用复筛液体培养基震荡培养时,其平均酶活力比出发菌株提高187%,达到145 500 U/L,且产酶稳定。
The mycelium fragments of Ganoderma lucidum were exposed successively under ultraviolet ray,nitrosoguanidine, ultraviolet ray-nitrosoguanidine and the mutants were screened in guaiacol-containing SPDA plates, and then the laccase activities of the mutated strains were determined by guaiacol. A mutant LYL263 with high yield of laccase was obtained. The average activity of laccase from LYL263 had increased up to 145 500 U/L,and its ability of producing laccase was stable on culture condition of liquid medium with glucose and yeast extract.
The mycelium fragments of Ganoderma lucidum were exposed successively under ultraviolet ray,nitrosoguanidine, ultraviolet ray-nitrosoguanidine and the mutants were screened in guaiacol-containing SPDA plates, and then the laccase activities of the mutated strains were determined by guaiacol. A mutant LYL263 with high yield of laccase was obtained. The average activity of laccase from LYL263 had increased up to 145 500 U/L,and its ability of producing laccase was stable on culture condition of liquid medium with glucose and yeast extract.
引文
[1]钞亚鹏,钱世钧.真菌漆酶及其应用[J].生物工程进展,2001,21(5):23-27
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[4]胡敏珊,张义正,曾凡亚.粗毛栓菌(Trametes gallica)基因启动子的分离与鉴定[J].四川大学学报:自然科学版,2002,39(2):225-229
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[8]谌斌,赵亮亮,聂小琦,等.组成型漆酶高产菌株的筛选及其产酶条件研究[J].广西师范大学学报:自然科学版,2009,27(2):76-79
[9]谌斌,赵亮亮,孙秋芳,等.红芝所产漆酶对蒽醌染料的脱色研究[J].环境科学与技术,2010,33(11):50-53.
[10]李青青,陈启和,蒋孝燕,等.基因工程菌EGs2产β-葡聚糖酶条件优化及产酶特性[J].农业生物技术学报,2007,15(4):708-712
[11]章名春.工业微生物育种[M].上海:上海科技出版社,1990:108-323
[2]张晓昱,黄慧艳.漆酶在食品工业中的研究与应用进展[J].食品科技,2003(4):4-7
[3]Brijwani K,Rigdon A,Vadlani P V.Fungal Laccases:production,function,and applications in food processing[J].Enzyme Research,2010,ID:149748
[4]胡敏珊,张义正,曾凡亚.粗毛栓菌(Trametes gallica)基因启动子的分离与鉴定[J].四川大学学报:自然科学版,2002,39(2):225-229
[5]刘淑珍,钱世钧.担子菌漆酶的分离纯化及其性质研究[J].微生物学报,2003,43(1):73-78
[6]朱陶,赵永芳.粗毛栓菌漆酶的分离纯化及部分性质研究[J].武汉大学学报:理学版,2002,48(2):209-212
[7]钞亚鹏,叶军,钱世钧.担子菌组成型漆酶产生特性的研究[J].微生物学报,2000,40(6):628-632
[8]谌斌,赵亮亮,聂小琦,等.组成型漆酶高产菌株的筛选及其产酶条件研究[J].广西师范大学学报:自然科学版,2009,27(2):76-79
[9]谌斌,赵亮亮,孙秋芳,等.红芝所产漆酶对蒽醌染料的脱色研究[J].环境科学与技术,2010,33(11):50-53.
[10]李青青,陈启和,蒋孝燕,等.基因工程菌EGs2产β-葡聚糖酶条件优化及产酶特性[J].农业生物技术学报,2007,15(4):708-712
[11]章名春.工业微生物育种[M].上海:上海科技出版社,1990:108-323