油红O染色在斑马鱼体内脂质染色中的应用

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英文篇名：Application of Oil Red O in the staining of lipids in Zebrafish 作者：陈侃 ; 王长谦 ; 范虞琪 ; 韩志华 ; 汪月 ; 高霖 ; 曾华甦 英文作者：Chen Kan;Wang Changqian;Fan Yuqi;Han Zhihua;Wang Yue;Gao Lin;Zeng Huasu;Department of Cardiology,Shanghai Ninth People's Hospital Affiliated to Shanghai Jiaotong University School of Medicine; 关键词：斑马鱼 ; 脂质 ; 油红O染色 英文关键词：Zebrafish;;lipids;;Oil Red O staining 中文刊名：GGZZ 英文刊名：Chinese Journal of Histochemistry and Cytochemistry 机构：上海交通大学医学院附属第九人民医院心内科; 出版日期：2016-11-01 08:47 出版单位：中国组织化学与细胞化学杂志 年：2016 期：v.25 基金：上海市进一步加快中医药事业发展三年行动计划(2014年-2016年)(ZY3-CCCX-3-3002);; 国家自然科学基金(81500331) 语种：中文; 页：GGZZ201604014 页数：3 CN：04 ISSN：42-1300/Q 分类号：64-66

摘要

目的探索油红O在斑马鱼体内脂质染色中的技术方法。方法取受精后4d的斑马鱼幼鱼,4%多聚甲醛固定12h后,用油红0工作液对整鱼进行染色,比较不同的染色时间及不同的染料浓度对油红O染色效果的影响。结果斑马鱼脑组织、卵黄囊、鱼鳔及血管内的脂质被油红O染色。油红0液浓度0.3%、染色时间3h时斑马鱼整鱼油红O染色效果较好。结论油红O可对斑马鱼体内的脂质进行染色,可用于斑马鱼脂代谢研究。
        Objective To explore the technical characteristics of Oil Red O staining of lipids in zebrafish.Methods 4d- post- fertilization zebrafish larvae were fixed in 4%paraformaldehyde for 12 h and then dyed with Oil Red 0.The effects of different dyeing time lengths and dye concentrations on the staining result were compared.Results The lipids in the brain tissue,yolk sacs,swim bladders and blood vessels of zebrafish were able to be stained with Oil Red 0.The best result was observed when the larve was stained with 0.3%Oil Red 0 for 3h.Conclusion Oil Red O is able to stain the lipids in zebrafish and be used for the study of lipid metabolism in zebrafish.

引文

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