响应曲面法优化精氨酸单糖苷合成工艺
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摘要
利用响应曲面法对苹果酸催化合成精氨酸单糖苷(AF)条件进行优化。本文在单因素的基础上,以精氨酸单糖苷的合成率为考察指标,建立以反应时间、反应温度、料液比和苹果酸加入量的4因素3水平的实验模型,采用Design Expert 8.0.6分析软件对精氨酸单糖苷的合成条件进行优化研究。结果表明,合成AF的最佳工艺条件,反应温度为85℃,反应时间150 min,料液比1∶20 g/m L,苹果酸加入量为0.75 g。优化出来的最佳合成条件稳定可行、合成率高。
The synthesis conditions of Arginy- Fructose( AF) were optimized by response surface methodology,and malic acid as catalyst. On the basis of single factor,the experimental models of 4 factors and 3 levels was used in this test with AF synthetic ratio as selection standard. Optimizing the synthesis condition of AF was simulated by Design Expert 8. 0. 6. The best synthesis condition of AF was received with 0. 75 g malic acid,85℃,150 min and 1∶ 20 g / m L solvent ratio. This preparation process of AF has the advantages of high synthesis rate and established.
The synthesis conditions of Arginy- Fructose( AF) were optimized by response surface methodology,and malic acid as catalyst. On the basis of single factor,the experimental models of 4 factors and 3 levels was used in this test with AF synthetic ratio as selection standard. Optimizing the synthesis condition of AF was simulated by Design Expert 8. 0. 6. The best synthesis condition of AF was received with 0. 75 g malic acid,85℃,150 min and 1∶ 20 g / m L solvent ratio. This preparation process of AF has the advantages of high synthesis rate and established.
引文
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[4]Gao F F.Zhang W Y,Liu L M,et al.Detection and distribution of arginine derivatives in Panax quin-quefolius L.and investigations of their antioxidant properties[J].Lebenson Wiss Technol,2012,49:34-41.
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[12]Lee K H,Ha K S,Jo S H,et al.Effect of Long-Term Dietary Arginyl-Fructose(AF)on Hyperglycemia and Hb A1c in Diabetic db/db Mice[J].Mol.Sci.2014,15,8352-8359.
[13]赵立春,杨更亮.响应曲面法在中药有效成分提取中的应用研究[J].中药与临床,2013,4(6):62-64.
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[15]王金玺,包振伟,刘笑,等.响应曲面法优化平菇多糖提取工艺[J].中国酿造,2013,23(40):108-112.
[16]柴婧,付警辉,张卫鹏,等.响应曲面法优化酶辅助闪式提取二氢槲皮素工艺[J].食品科学,2014,35(10):93-97.
[17]吴燕,盛尊来,高凌飞,等.响应面法优化丁香叶总酚酸提取工艺[J].食品工业科技,2015,36(2):286-290.
[18]钱丹丹,李新华,杨强,等.响应面分析法优化水飞蓟多糖提取工艺[J].食品工业,2015,36(6):117-120.
[19]朱盛林,郑玲利,袁明勇,等.响应面法优化附子多糖的微博提取工艺[J].中国医药导报,2015,12(16):48-51.
[20]王健,曾吉祥,龚远林.响应曲面法优化姜黄素包合物的制备[J].川北医学院学报,2015,30(2):166-169.
[21]吴军林,吴清平,张菊梅,等.L-苹果酸生物合成研究进展[J].食品科学,2014,35(2):238-242.
[2]Zheng Y N,Okuda H,Han L K,et al.A new amino acid derivative from red ginseng[J].Acta Pharmaceutica Sinica(B),1998,7:7-10.
[3]Takaku T,Han,L K,Kameda K,et al.Production of arginylfrictosyl glucose during processing of red ginseng[J].J.Tradit.Med,1996,13,118-123.
[4]Gao F F.Zhang W Y,Liu L M,et al.Detection and distribution of arginine derivatives in Panax quin-quefolius L.and investigations of their antioxidant properties[J].Lebenson Wiss Technol,2012,49:34-41.
[5]郑毅男.杨世杰.精氨酸糖苷的制备方法[P].CN,ZL201110038843.7.2012-07-04.
[6]Suzuki Y,Choi,K J,Uchida K,et al.Arginyl-fructosyl-glucose and arginyl-fructose,compounds related to browning reaction in the model system of steaming and heat-drying processes for the preparation of red ginseng[J].Ginseng Res,2004,28,143-148.
[7]Cho E,Piao X L,Jang M H,et al.The effect of steaming on the free amino acid contents and antioxidant activity of Panax ginseng.J.Food Chem.2008,107:876-882.
[8]Lee J S,Kim G N,Lee S H,et al.In vitro and cellular antioxidant activity of arginyl-fructose and arginyl-fructosyl-glucose[J].Food Science and Biotechnology,2009,18(6),1505-1510.
[9]Keum Y S,Park K K,Lee J M,et al.Antioxidant and antitumor promoting activities of the methanol extract of heat-processed ginseng[J].Cancer Lett,2000,150,41-48.
[10]郑毅男,张晶,奥田拓道,等.精氨酸衍生物对微循环的作用[J].吉林农业大学学报,1998,20(4):45-47.
[11]赵婷.人工合成L-精氨酸单糖苷(AF)及其抗糖尿病药理活性研究[D].长春:吉林农业大学,2013,16-22.
[12]Lee K H,Ha K S,Jo S H,et al.Effect of Long-Term Dietary Arginyl-Fructose(AF)on Hyperglycemia and Hb A1c in Diabetic db/db Mice[J].Mol.Sci.2014,15,8352-8359.
[13]赵立春,杨更亮.响应曲面法在中药有效成分提取中的应用研究[J].中药与临床,2013,4(6):62-64.
[14]于淼,柏云娇,代岐昌,等.响应曲面法优化文殊兰中生物碱的提取工艺[J].中草药,2013,44(10):1286-1289.
[15]王金玺,包振伟,刘笑,等.响应曲面法优化平菇多糖提取工艺[J].中国酿造,2013,23(40):108-112.
[16]柴婧,付警辉,张卫鹏,等.响应曲面法优化酶辅助闪式提取二氢槲皮素工艺[J].食品科学,2014,35(10):93-97.
[17]吴燕,盛尊来,高凌飞,等.响应面法优化丁香叶总酚酸提取工艺[J].食品工业科技,2015,36(2):286-290.
[18]钱丹丹,李新华,杨强,等.响应面分析法优化水飞蓟多糖提取工艺[J].食品工业,2015,36(6):117-120.
[19]朱盛林,郑玲利,袁明勇,等.响应面法优化附子多糖的微博提取工艺[J].中国医药导报,2015,12(16):48-51.
[20]王健,曾吉祥,龚远林.响应曲面法优化姜黄素包合物的制备[J].川北医学院学报,2015,30(2):166-169.
[21]吴军林,吴清平,张菊梅,等.L-苹果酸生物合成研究进展[J].食品科学,2014,35(2):238-242.