虎源大肠杆菌ESBLs基因型的检测
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摘要
为检测虎源大肠杆菌的耐药性,根据超广谱β-内酰胺酶(ESBLs)基因型的流行情况指导临床合理使用抗菌药物,有效控制产ESBLs菌株的流行和感染。实验采用K-B法检测了30株虎源大肠杆菌对20种药物的耐药性,同时用PCR方法对确证的产ESBLs大肠杆菌进行TEM、CTX-M、OXA和SHV基因型检测。结果显示:21株虎源大肠杆菌为产ESBLs株,阳性率为70%。PCR扩增出了片段大小与预期相符的CTX-M型、OXA型、SHV型和TEM型基因。4个基因型的阳性率分别为50%、20%、6. 67%、80%。可见,东北虎林园中产ESBLs的虎源大肠杆菌的检出率较高,TEM型为主要流行基因型。另外,发现产酶大肠杆菌比非产酶大肠杆菌的耐药性更高。
This study tested the antibiotic resistance of Escherichia coli from tiger,developed guidance on the proper clinical use of antibiotics,and effectively controlled the infection of strains with extended-spectrum β-lactamase( ESBL) genotypes. We tested drug resistance of 30 strains from tiger to 20 drugs by use of the KB method. E. coli with confirmed ESBLs genotype were tested for genotype of CTX-M,OXA,SHV and TEM by use of the PCR method. Twenty-one of the 30 strains were confirmed ESBL positive. The positive rate was 70%. Four different genotypes were detected among these ESBL isolates by PCR. The positive rates of genotypes CTX-M,OXA,SHV and TEM were 50%,20%,6. 7% and 80%, respectively. Tiger-derived ESBL-positive strain showed high positive rates in Northeast Tiger Park,Harbin,and TEM was the most common genotype. ESBL-positive strains showed higher drug resistance than negative ones.
This study tested the antibiotic resistance of Escherichia coli from tiger,developed guidance on the proper clinical use of antibiotics,and effectively controlled the infection of strains with extended-spectrum β-lactamase( ESBL) genotypes. We tested drug resistance of 30 strains from tiger to 20 drugs by use of the KB method. E. coli with confirmed ESBLs genotype were tested for genotype of CTX-M,OXA,SHV and TEM by use of the PCR method. Twenty-one of the 30 strains were confirmed ESBL positive. The positive rate was 70%. Four different genotypes were detected among these ESBL isolates by PCR. The positive rates of genotypes CTX-M,OXA,SHV and TEM were 50%,20%,6. 7% and 80%, respectively. Tiger-derived ESBL-positive strain showed high positive rates in Northeast Tiger Park,Harbin,and TEM was the most common genotype. ESBL-positive strains showed higher drug resistance than negative ones.
引文
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[17]苑丽,刘建华,胡功政,等.鸡大肠杆菌TEM和CTX-M型超广谱β-内酰胺酶基因分型研究[J].中国农业科学,2010,43(20):4310-4316.
[18]Liao X P,Liu B T,Yang Q E,et al.Comparison of plasmids coharboring 16S rRNA methylase and extended-spectrumβ-lactamase genes among Escherichia coli isolates from pets and poultry[J].Journal of Food Protection,2013,76(12):2018-2023.
[19]Livermore D M,Canton R,Gniadkowski M,et al.CTX-M:changing the face of ESBLs in Europe[J].Journal of Antimicrobial Chemotherapy,2007,59(2):165-174.
[20]许颖,李晓庆,江俊,等.产ESBLs大肠埃希菌耐药基因分型研究[J].四川医学,2009,30(9):1363-1365.
[21]Zong Z Y,LüX J,Valenzuela J K,et al.An outbreak of carbapenem-resistant Acinetobacter baumannii producing OXA-23 carbapenemase in western China[J].International Journal of Antimicrobial Agents,2008,31(1):50-54.
[2]陈体,伍勇.整合子介导超广谱β-内酰胺酶的研究进展[J].实验与检验医学,2009,27(3):297-299;311.
[3]黄瑞娟,叶临湘,冯启明.大肠埃希菌产超广谱β-内酰胺酶危险因素病例对照研究[J].中国感染控制杂志,2007,6(4):235-238.
[4]肖永红;王进;赵彩云.2006-2007年Mohnarin细菌耐药监测[J].中华医院感染学杂志,2008,18(8):1051-1056.
[5]汪复,朱德妹,胡付品,等.2008年中国CHINET细菌耐药性监测[J].中国感染与化疗杂志,2009,9(5):321-329.
[6]牛鑫鑫,刘闯,唐连友,等.鹤源大肠杆菌ESBLs基因型检测及其耐药性分析[J].经济动物学报,2014,18(2):104-106;110.
[7]Bush K,Jacoby G A,Medeiros A A.A functional classification scheme for beta-lactamases and its correlation with molecular structure[J].Antimicrobial Agents and Chemotherapy,1995,39(6):1211-1233.
[8]邱文影,钟国权.产超广谱β-内酰胺酶大肠埃希菌和肺炎克雷伯菌的耐药性分析[J].中华医院感染学杂志,2005,15(12):1415-1416.
[9]Yu W L,Chuang Y C,Walther-Rasmussen J.Extended-spectrum beta-lactamases in Taiwan:epidemiology,detection,treatment and infection control[J].Journal of Microbiology,Immunology,and Infection,2006,39(4):264-277.
[10]梁贤球,潘树根.大肠埃希菌感染的临床分布与耐药性[J].中国抗感染化疗杂志,2001,1(1):41-42.
[11]Clinical and Laboratory Standards Institute(CLSI).Performance standards for antimicrobial susceptibility testing[C].16th Informational Supplement.Document M100-S16,Wayne,PA:CLSI,2009:15-19.
[12]Van T T H,Chin J,Chapman T,et al.Safety of raw meat and shellfish in Vietnam:an analysis of Escherichia coli isolations for antibiotic resistance and virulence genes[J].International Journal of Food Microbiology,2008,124(3):217-223.
[13]胡同平,张文兰,张永梅.大肠埃希菌产超广谱β-内酰胺酶株的基因型研究[J].中华医院感染学杂志,2009,19(17):2239-2241.
[14]罗燕萍,张秀菊,徐雅萍,等.产超广谱β-内酰胺酶肺炎克雷伯菌和大肠埃希菌的分布及其耐药性研究[J].中华医院感染学杂志,2006,16(1):101-104.
[15]Fortini D,Fashae K,García-Fernández A,et al.Plasmid-mediated quinolone resistance andβ-lactamases in Escherichia coli from healthy animals from Nigeria[J].Journal of Antimicrobial Chemotherapy,2011,66(6):1269-1272.
[16]Guenther S,Aschenbrenner K,Stamm I,et al.Comparable high rates of extended-spectrum-beta-lactamase-producing Escherichia coli in birds of prey from Germany and Mongolia[J].PLo S One,2012,7(12):e53039.
[17]苑丽,刘建华,胡功政,等.鸡大肠杆菌TEM和CTX-M型超广谱β-内酰胺酶基因分型研究[J].中国农业科学,2010,43(20):4310-4316.
[18]Liao X P,Liu B T,Yang Q E,et al.Comparison of plasmids coharboring 16S rRNA methylase and extended-spectrumβ-lactamase genes among Escherichia coli isolates from pets and poultry[J].Journal of Food Protection,2013,76(12):2018-2023.
[19]Livermore D M,Canton R,Gniadkowski M,et al.CTX-M:changing the face of ESBLs in Europe[J].Journal of Antimicrobial Chemotherapy,2007,59(2):165-174.
[20]许颖,李晓庆,江俊,等.产ESBLs大肠埃希菌耐药基因分型研究[J].四川医学,2009,30(9):1363-1365.
[21]Zong Z Y,LüX J,Valenzuela J K,et al.An outbreak of carbapenem-resistant Acinetobacter baumannii producing OXA-23 carbapenemase in western China[J].International Journal of Antimicrobial Agents,2008,31(1):50-54.