升麻对牙菌斑主要组成链球菌的体外抑菌活性研究
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摘要
目的:研究中药升麻提取物对牙菌斑主要组成链球菌的抑制活性。方法:使用乙醇回流提取升麻有效成分,采用微量肉汤稀释法测定升麻提取物对变异链球菌(Streptococcus mutans)、血链球菌(Streptococcus sanguis)、唾液链球菌(Streptococcus saliva)和乙型溶血性链球菌(β-Hemolytic streptococcus)4种牙菌斑主要组成链球菌的最小抑菌浓度(MIC)。结果:升麻提取液对4种细菌均有抑菌作用,其中对变异链球菌和唾液链球菌抑制活性明显强于乙型溶血链球菌和血链球菌。结论:升麻提取液对变异链球菌和唾液链球菌的抑制活性显著,具有进一步研究升麻提取液对牙菌斑形成是否具有抑制活性的潜力。
Objective:To investigate the inhibitory activity of Cimicifuga extract on the major streptococcus in the dental plaque.Methods:The active constituents of Cimicifuga were extracted by refluxing with ethanol,and the minimum inhibitory concentration(MIC) of Cimicifuga extract against 4 types major streptococcus in dental plaque including streptococcus mutan,streptococcus sanguis,streptococcus saliva and β-Hemolytic streptococcus were determined by micro-broth dilution.Results:Cimicifuga extract showed certain inhibitory activity against Streptococcus mutans and Streptococcus salivarius, and weaker inhibitory activity against Streptococcus mutans and Streptococcus sanguis.Conclusion:The Cimicifuga extract has significant inhibitory activity against Streptococcus mutans and Streptococcus saliva, and has the potential to further study whether the Cimicifuga extract has inhibitory activity on plaque formation.
Objective:To investigate the inhibitory activity of Cimicifuga extract on the major streptococcus in the dental plaque.Methods:The active constituents of Cimicifuga were extracted by refluxing with ethanol,and the minimum inhibitory concentration(MIC) of Cimicifuga extract against 4 types major streptococcus in dental plaque including streptococcus mutan,streptococcus sanguis,streptococcus saliva and β-Hemolytic streptococcus were determined by micro-broth dilution.Results:Cimicifuga extract showed certain inhibitory activity against Streptococcus mutans and Streptococcus salivarius, and weaker inhibitory activity against Streptococcus mutans and Streptococcus sanguis.Conclusion:The Cimicifuga extract has significant inhibitory activity against Streptococcus mutans and Streptococcus saliva, and has the potential to further study whether the Cimicifuga extract has inhibitory activity on plaque formation.
引文
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[2] 葛玉琰.兴安升麻抗病毒活性及其质量标准研究[D].哈尔滨:哈尔滨工商大学.
[3] 李立方,张勇.中药对牙周致病厌氧菌体外抑菌活性的研究[J].河北中医药学报,2008,23(4):30-31.
[4] 胡桂清,李建志,王黑力,等.五种地产中药抗菌作用的实验研究[J].中医药学报,1993,(3):40.
[5] 刘天佳.口腔疾病的微生物学基础[M].北京:人民卫生出版社,1999.
[6] 高鲁胶,朱德全.口腔科病房感染的病原体调查及耐药性分析[J].中华医院感染学杂志,2004,14(5):119-121.
[7] 李建志,刘旭红,杨丽珍,等.11种中草药抗菌作用实验研究[J].中医药信息,2009,26(3):82-83.
[8] 郑雅楠.含升麻、黄连和花椒等的口腔护理剂用于防治牙周病等[J].国外医药:植物药分册,2004,19(2):83.
[9] 王记祥,金首文.南川升麻的化学成分和抗菌活性研究[J].安徽农业科学,2012,40(5):2651-2653.
[10] 刘玉梅.五倍子和升麻抗龋病相关口腔致病菌活性物质的筛选及作用机理研究[D].昆明:昆明理工大学,2016.
[11] 胡小艳.22种中药对常见口腔致病菌的体外抑菌实验[J].中国保健营养,2016,26(25):234-235.