玉屏风多糖组分对皮肤角质形成细胞的作用研究
|
摘要
目的研究中药方剂玉屏风的多糖组分在体外对角质形成细胞的免疫调节作用。方法利用Real-time PCR法检测玉屏风多糖组分对激活的皮肤角质形成细胞中各炎症因子表达的调控作用;分别用Real-time PCR法和Elisa法对聚角蛋白微丝蛋白(Filaggrin)在mRNA和蛋白水平的表达进行检测;用Western bolt法检测多糖组分对NF-κB信号通路的调节作用。结果玉屏风多糖组分明显抑制激活的皮肤角质形成细胞分泌炎症因子,促进Filaggrin的表达,并对NF-κB信号通路有明显的抑制作用,该信号通路对炎症和免疫细胞的活化有重要作用。结论玉屏风多糖组分在体外对皮肤角质形成细胞有免疫调节作用,能够减缓皮肤局部炎症的发展,为玉屏风方剂的进一步开发应用奠定理论基础。
Objective To study the immunomodulatory effect of Yupingfeng polysaccharides on keratinocytes in vitro.Methods The effects of Yupingfeng polysaccharides on the expression of various inflammatory factors in the activated keratinocytes were detected by the Real-time PCR method.The mRNA and protein expression levels of Filaggrin were detected by Real-time PCR and Elisa respectively.The western bolt assay was used to detect the regulation of NF-κB signaling pathway by polysaccharides.Results Yupingfeng polysaccharides inhibited the secretion of inflammatory factors in the activated keratinocytes,promoted the expression of Filaggrin and exhibited significant inhibitory effect on the NF-κB signaling pathway,which plays an important role in the inflammation and immune response.Conclusion Yupingfeng polysaccharides had an immune regulation effect on skin keratinocytes in vitro and alleviated the development of local inflammation.These results lay a theoretical foundation for the clinical application of Yupingfeng prescription.
Objective To study the immunomodulatory effect of Yupingfeng polysaccharides on keratinocytes in vitro.Methods The effects of Yupingfeng polysaccharides on the expression of various inflammatory factors in the activated keratinocytes were detected by the Real-time PCR method.The mRNA and protein expression levels of Filaggrin were detected by Real-time PCR and Elisa respectively.The western bolt assay was used to detect the regulation of NF-κB signaling pathway by polysaccharides.Results Yupingfeng polysaccharides inhibited the secretion of inflammatory factors in the activated keratinocytes,promoted the expression of Filaggrin and exhibited significant inhibitory effect on the NF-κB signaling pathway,which plays an important role in the inflammation and immune response.Conclusion Yupingfeng polysaccharides had an immune regulation effect on skin keratinocytes in vitro and alleviated the development of local inflammation.These results lay a theoretical foundation for the clinical application of Yupingfeng prescription.
引文
[1]LEUNG D Y,BIEBER T.Atopic dermatitis[J].Lancet,2003,361(9352):151-160.
[2]LEUNG D Y,BOGUNIEWICZ M,HOWELL M D,et al.New insights into atopic dermatitis[J].J Clin Invest,2004,113(5):651-657.
[3]GITTLER J K,KRUEGER J G,GUTTMAN-YASSKY E.Atopic dermatitis results in intrinsic barrier and immune abnormalities:Implications for contact dermatitis[J].Allergy Clin Immunol,2013,131(2):300-313.
[4]葛延全.玉屏风散应用于皮肤疾病经验[J].内蒙古中医药,2001,20(2):20.
[5]李雪娇.特异性免疫治疗联合玉屏风颗粒治疗特应性皮炎的疗效观察[J].山东大学学报(医学版),2011,49(7):144-146.
[6]韩莉.玉屏风散配合西药治疗慢性湿疹60例[J].陕西中医,2015,36(3):322-324.
[7]胡建农.玉屏风胶囊合复方甘草酸苷片治疗慢性荨麻疹48例[J].现代中西医结合杂志,2013,22(3):268-269.
[8]陈静,袁明勇,郑玲利,等.黄芪的化学成分和药理作用研究[J].临床医药实践,2009,18(11):2217-2219.
[9]KAJIMURA K,TAKAGI Y,MIYANO K,et al.Polysaccharide of Astragali radix enhances IgM antibody production in aged mice[J].Biol Pharm Bull,1997,20(11):1178-1182.
[10]曹岗,张晓炎,丛晓东,等.白术多糖的研究进展[J].北京联合大学学报(自然科学版),2009,23(3):14-18.
[11]杨淳,田维毅.防风多糖对巨噬细胞分泌细胞因子的影响[J].贵阳中医学院学报,2011,33(4):31-33.
[12]WEIDINGER S,ILLIG T,BAURECHT H,et al.Loss-offunction variations within the Filaggrin gene predispose for atopic dermatitis with allergic sensitizations[J].J Allergy Clin Immunol,2006,118(1):214-219.
[2]LEUNG D Y,BOGUNIEWICZ M,HOWELL M D,et al.New insights into atopic dermatitis[J].J Clin Invest,2004,113(5):651-657.
[3]GITTLER J K,KRUEGER J G,GUTTMAN-YASSKY E.Atopic dermatitis results in intrinsic barrier and immune abnormalities:Implications for contact dermatitis[J].Allergy Clin Immunol,2013,131(2):300-313.
[4]葛延全.玉屏风散应用于皮肤疾病经验[J].内蒙古中医药,2001,20(2):20.
[5]李雪娇.特异性免疫治疗联合玉屏风颗粒治疗特应性皮炎的疗效观察[J].山东大学学报(医学版),2011,49(7):144-146.
[6]韩莉.玉屏风散配合西药治疗慢性湿疹60例[J].陕西中医,2015,36(3):322-324.
[7]胡建农.玉屏风胶囊合复方甘草酸苷片治疗慢性荨麻疹48例[J].现代中西医结合杂志,2013,22(3):268-269.
[8]陈静,袁明勇,郑玲利,等.黄芪的化学成分和药理作用研究[J].临床医药实践,2009,18(11):2217-2219.
[9]KAJIMURA K,TAKAGI Y,MIYANO K,et al.Polysaccharide of Astragali radix enhances IgM antibody production in aged mice[J].Biol Pharm Bull,1997,20(11):1178-1182.
[10]曹岗,张晓炎,丛晓东,等.白术多糖的研究进展[J].北京联合大学学报(自然科学版),2009,23(3):14-18.
[11]杨淳,田维毅.防风多糖对巨噬细胞分泌细胞因子的影响[J].贵阳中医学院学报,2011,33(4):31-33.
[12]WEIDINGER S,ILLIG T,BAURECHT H,et al.Loss-offunction variations within the Filaggrin gene predispose for atopic dermatitis with allergic sensitizations[J].J Allergy Clin Immunol,2006,118(1):214-219.