中国近海藻毒素及有毒微藻产毒原因种调查研究进展
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摘要
麻痹性贝类毒素在我国近海污染问题已十分突出,基本呈现逐年加剧的趋势。20世纪90年代,南海麻痹性贝类毒素污染较重; 21世纪初,北黄海麻痹性贝类毒素污染较重;近几年,渤海和福建近海麻痹性贝类毒素污染较重;可产生麻痹性贝类毒素的微藻有亚历山大藻和裸甲藻等。采用小鼠生物法检测我国近海腹泻性贝类毒素超标率32%左右,采用液相色谱/质谱法检测,仅有3起超标的研究报道;现行小鼠生物法检测腹泻性贝类毒素假阳性问题十分突出,应尽快废除;腹泻性贝类毒素均是脂溶性的,脂溶性海洋生物毒素在我国近海常年可检出,偶有虾夷扇贝毒素和鳍藻毒素超标现象。可产生脂溶性毒素微藻有鳍藻和原甲藻及网状原角藻等。失忆性贝类毒素在我国近海常有检出,但无超标现象;产毒微藻有拟菱形藻等。西加鱼毒素在我国南海污染较重,但毒素标准物质的匮乏,限制了西加鱼毒素的调查研究;至今尚未确定产生西加鱼毒素的微藻。酶联免疫吸附法和液相色谱串联质谱法相结合已成为藻毒素快速准确检测成熟的技术,逐渐代替小鼠生物法和液相色谱法。
Phycotoxins and toxic microalgae have been investigated since early 1990 s in coastal waters of China.Paralytic shellfish poisoning(PSP) pollution has been increasing year by year, which seriously harmed the coastal waters in South China Sea in 1990 s, in the northern Yellow Sea in early 2000 s, and in the Qinhuangdao and Fujian recently. PSP had been found produced by toxic species of genus Alexandrium and Gymnodinium catenatum. Diarrhetic shellfish poisoning(DSP) in the coastal waters of China has been increasing and about 32% of the samples exceeded the safety benchmark determined by mouse bioassay but only three cases were detected by HPLC and LC-MS/MS methods.Therefore, mouse bioassay for DSP test in China produced obviously false positive results and should be repealed.Lipophilic marine biotoxins have been detected in the coastal waters of China in all seasons, and the levels of yessotoxin(YTX) and dinophysistoxin-1(DTX1) were occasionally reported high beyond the safety point. The lipophilic toxins have been found produced mainly by toxic Dinophysis spp, Prorocentrum spp. Azadinium spp. Protoceratium reticulatum, and Vulcanodinium rugosum. Domoic acid(DA), the main component of amnesic shellfish poisoning(ASP), had been confirmed secreted from diatom Pseudo-nitzschia simulans that frequently occurred recently in the coastal waters of China but remained within the safe level. Recently, ciguatera fish poisoning(CFP) polluted seriously the coastal waters of South China Sea but no microalga has yet been found to produce the toxin. The ELISA and LC-MS/MS methods for detecting marine biotoxins have become mature methods and expected to replace gradually the MBA and HPLC methods in China.
Phycotoxins and toxic microalgae have been investigated since early 1990 s in coastal waters of China.Paralytic shellfish poisoning(PSP) pollution has been increasing year by year, which seriously harmed the coastal waters in South China Sea in 1990 s, in the northern Yellow Sea in early 2000 s, and in the Qinhuangdao and Fujian recently. PSP had been found produced by toxic species of genus Alexandrium and Gymnodinium catenatum. Diarrhetic shellfish poisoning(DSP) in the coastal waters of China has been increasing and about 32% of the samples exceeded the safety benchmark determined by mouse bioassay but only three cases were detected by HPLC and LC-MS/MS methods.Therefore, mouse bioassay for DSP test in China produced obviously false positive results and should be repealed.Lipophilic marine biotoxins have been detected in the coastal waters of China in all seasons, and the levels of yessotoxin(YTX) and dinophysistoxin-1(DTX1) were occasionally reported high beyond the safety point. The lipophilic toxins have been found produced mainly by toxic Dinophysis spp, Prorocentrum spp. Azadinium spp. Protoceratium reticulatum, and Vulcanodinium rugosum. Domoic acid(DA), the main component of amnesic shellfish poisoning(ASP), had been confirmed secreted from diatom Pseudo-nitzschia simulans that frequently occurred recently in the coastal waters of China but remained within the safe level. Recently, ciguatera fish poisoning(CFP) polluted seriously the coastal waters of South China Sea but no microalga has yet been found to produce the toxin. The ELISA and LC-MS/MS methods for detecting marine biotoxins have become mature methods and expected to replace gradually the MBA and HPLC methods in China.
引文
于梅,闫鹏,徐景野,2004.宁波市1986~2003年织纹螺毒性检测结果分析.实用预防医学,11(6):1267-1268
王舟,黄薇,潘柳波等,2012.2011年深圳市水产品卫生状况及风险评估.中国公共卫生管理,28(5):595-597
王茜,程金平,高利利等,2012.软骨藻酸直接竞争ELISA方法的建立及优化.环境科学,33(2):647-651
王恒,2011.舟山海域贝类海产品中软骨藻酸含量调查.中国卫生检验杂志,21(12):2986-2988,2992
王金辉,秦玉涛,刘材材等,2007.长江口及邻近海域有毒藻类和赤潮毒素的本底调查.海洋湖沼通报,(1):52-61
王雪虹,黄世玉,2007.厦门海域养殖区麻痹性贝毒污染的研究.集美大学学报(自然科学版),12(2):114-117
勾玉晓,刘磊,李冬梅等,2018.北黄海慢原甲藻形态结构与腹泻性贝类毒素组成.中国渔业质量与标准,8(3):11-18
孔凡洲,徐子钧,于仁成等,2007.黄渤海海域贝类麻痹性贝毒的检测与分析.中国海洋大学学报,37(2):305-309
母清林,王晓华,佘运勇等,2013.浙江近岸海域贝类中重金属和贝毒污染状况研究.海洋科学,37(1):87-91
吉薇,郑洁莹,曾雪萍等,2011.南海海域软骨藻酸(DA)贝类毒素的HPLC方法检测.现代食品科技,27(1):120-122,116
刘丽,刘磊,赵芮等,2016.酶联免疫吸附分析检测鳍藻毒素DTX1和DTX2.分析科学学报,32(4):557-560
刘俏,龙丽娟,2013.南海慢原甲藻的培养和毒性初探.湖北农业科学,52(5):1113-1117
刘仁沿,许道艳,董玉华等,2009.海水和贝类中软骨藻酸的酶联免疫吸附分析方法研究.卫生研究,38(5):662-624
刘仁沿,陈冰君,梁玉波等,2008a.腹泻性贝毒软海绵酸单克隆抗体的制备和酶联免疫检测方法的建立.卫生研究,37(4):443-445
刘仁沿,高春蕾,梁玉波等,2008c.Gymnodimine,首次在我国北海缘齿牡蛎中发现的一种腹泻性贝毒组分.海洋学报,30(6):171-176
刘仁沿,梁玉波,刘磊等,2014.液相色谱结合串联质谱方法研究中国沿海贝类中脂溶性藻毒素的种类结构和分布规律.生态环境学报,23(8):1320-1326
刘仁沿,梁玉波,陈冰君等,2008b.胶体金免疫层析方法快速检测腹泻性贝毒软海绵酸的初步研究.分析试验室,27(7):26-29
刘淑娟,赵晓祥,程金平等,2014.快速检测软骨藻酸的间接ELISA方法.环境科学学报,34(2):404-408
关春江,冯志权,马明辉等,1999.长江以北沿海经济贝类中的麻痹性贝毒.海洋环境科学,18(2):49-52
江天久,尹伊伟,骆育敏等,2000b.广东大亚湾和大鹏湾麻痹性贝类毒素研究.中国环境科学,20(4):341-344
江天久,尹伊伟,黄伟建等,2000a.深圳大亚湾麻痹性贝类毒素成分特征.暨南大学学报(自然科学版),21(5):65-69
江天久,包财,雷芳等,2010.广东东部沿海麻痹性贝类毒素成分特征分析.中国水产科学,17(1):119-127
江天久,江涛,2007.中国沿海部分海域麻痹性贝毒研究.海洋与湖沼,38(1):36-41
江天久,陈菊芳,邹迎麟等,2003.中国东海和南海有害赤潮高发区麻痹性贝毒素研究.应用生态学报,14(7):1156-1160
许道艳,刘磊,刘仁沿等,2013.麻痹性贝毒单克隆抗体的制备和酶联免疫检测方法的建立.中国免疫学杂志,29(1):69-73
许道艳,刘仁沿,董玉华等,2007.失去记忆性贝毒ASP酶联免疫检测方法的研究.海洋环境科学,26(3):237-240
孙烨,刘威,郑剑等,2016.2011-2014年深圳市贝类产品生物毒素污染状况调查.检测研究,28(2):149-150,154
杜克梅,江天久,吴霓,2013a.黄海海域贝类麻痹性贝类毒素污染状况研究.海洋环境科学,32(2):182-184
杜克梅,雷芳,吴霓等,2013b.我国东海和南海近岸海域麻痹性贝类毒素污染状况.暨南大学学报(自然科学版),34(3):343-346
李大志,祝文君,朱文斌等,2002.记忆缺失性贝类毒素的主要成分--软骨藻酸的毛细管电泳分析.色谱,20(2):125-128
李伟才,栾刚,李立等,2000.中国沿海部分海区贝毒毒素的调查.海洋科学,24(9):19-23
李晓晶,彭荣飞,于鸿等,2013.广州市市售水产品中多种脂溶性贝类毒素的监测.中国卫生检验杂志,23(10):2317-2319
李嘉雯,江涛,吴锋等,2014.珠江口与大亚湾海域腹泻性贝毒污染状况分析.暨南大学学报(自然科学与医学版),35(3):228-234
杨莉,杨维东,刘洁生等,2006.广州市售贝类麻痹性贝毒和腹泻性贝毒污染状况分析.卫生研究,35(4):435-439
杨美兰,林钦,吕晓瑜等,2005.广东重要渔业水域牡蛎体中的麻痹性贝类毒素.海洋环境科学,24(1):48-50
杨美兰,林燕棠,全桂英,1999.广东沿海牡蛎体的麻痹性毒素与评价.湛江海洋大学学报,19(3):38-42
杨美兰,林燕棠,贾晓平等,2002.珠江口及邻近海域贝类麻痹性毒素调查.中国水产科学,9(3):283-285
杨美兰,贾晓平,林钦等,2009.南海海域重要养殖水域牡蛎体中的腹泻性贝类毒素.海洋环境科学,28(4):410-413
吴锋,江天久,张帆等,2010.浙江南麂列岛海域贝类中的麻痹性贝类毒素研究.海洋环境科学,29(3):360-363
吴施卫,卢楚谦,朱小山等,2005a.广东沿海麻痹性贝毒素的地理分布特征.海洋环境科学,24(3):40-43
吴施卫,张纯超,卢楚谦等,2005b.南海近岸海域腹泻性贝类毒素分析.海洋环境科学,24(4):48-51
吴施卫,曾淼,卢大鹏等,2008a.广东近岸海域2005年春季的腹泻性贝毒素特征分析.海洋环境科学,27(2):165-168,181
吴施卫,曾淼,卢大鹏等,2008b.2005年春季广东近岸海域麻痹性贝毒素的特征分析.海洋学研究,26(4):49-54
何依娜,金雷,梅光明等,2016.舟山市海水养殖贝类质量安全现状评价.安徽农业科学,44(27):122-123,203
冷科明,吴霓,杜克梅等,2013.粤西海域麻痹性贝类毒素成分特征分析.生态科学,32(5):558-563
冷科明,吴霓,杜克梅等,2014.粤中海域麻痹性贝类毒素成分特征分析.海洋环境科学,33(5):666-671
宋向明,谢小华,赵冲厚等,2013.酶联免疫法测定牡蛎中腹泻性毒素的研究.水产养殖,34(1):49-52
宋琍琍,张海琪,侯镜德等,2008.液相色谱-串联质谱法测定贝类毒素软骨藻酸的残留.水产学报,32(6):950-956
宋普江,张伟,王刚等,2011.大连近岸海域经济贝类麻痹性贝毒分析.海洋环境科学,30(4):533-535,545
宋新成,周德山,尹华斌等,2016.海州湾近海海域海水中贝类毒素的检测与季节变化.淮海工学院学报(自然科学版),25(3):83-87
张秀尧,蔡欣欣,2012.从暴发腹泻的贻贝中同时检出腹泻性贝类毒素和扇贝毒素.中国卫生检验杂志,22(8):1905-1907,1911
张树刚,邹清,陈雷等,2011.浙南海域腹泻性贝毒分析.海洋科学,35(1):44-47,53
陈西平,王成斌,胡俊明等,2001.HPLC方法检测水及水生动物中软骨藻酸.卫生研究,30(4):247-248
陈建华,于仁成,孔凡洲等,2014.北黄海海域虾夷扇贝体内脂溶性藻毒素分析.海洋与湖沼,45(4):855-863
林祥田,张明生,王志坚等,2005.连云港海州湾麻痹性贝类毒素中毒分析.中国食品卫生杂志,17(3):243-246
林燕棠,杨美兰,陈瑞雯等,1994.广东沿海麻痹性贝类毒素的研究.海洋与湖沼,25(2):220-225
林燕棠,贾晓平,杨美兰等,1999.中国沿岸染毒贝类的麻痹性毒素.热带海洋,18(1):90-96
林燕棠,贾晓平,杨美兰等,2001.我国海产贝类体中的麻痹性毒素及其来源.水产学报,25(5):479-481
罗璇,于仁成,周名江,2014.应用LC-MS联用方法分析青岛近海渐尖鳍藻(Dinophysis acuminata)细胞中的毒素成分.海洋环境科学,33(5):781-787
郑仁锦,李榕珊,黄宏南等,2016.福建省市售水产品中河豚毒素及腹泻性贝类毒素污染状况.职业与健康,32(17):2367-2370
郑重莺,张海琪,宋琍琍等,2012.浙江省市售主要食用贝类中麻痹性贝类毒素和腹泻性贝类毒素污染状况分析.浙江农业科学,(2):236-239
赵芮,刘磊,刘丽等,2015.酶联免疫吸附法和液相色谱-质谱联用法分析海洋生物中记忆缺失性贝毒.分析试验室,34(8):882-885
胡蓉,江天久,2013.海南岛近岸海域贝类中的腹泻性贝类毒素.暨南大学学报(自然科学版),34(5):527-532
胡颢琰,唐静亮,王益鸣等,2006.浙江近岸有害赤潮发生区麻痹性贝毒素研究.海洋环境科学,25(1):63-65
姚建华,谭志军,周德庆等,2010.液相色谱-串联质谱法检测贝类产品中的原多甲藻酸贝类毒素.色谱,28(4):363-367
夏远征,王双双,辛丘岩等,2010.大连海域贝类麻痹性贝毒的污染状况调查与分析.食品与机械,26(2):54-56
徐奋奋,吴蓓莉,黄亚琴等,2013.宁波市市售海水产品腹泻性贝类毒素检测结果分析.中国卫生检验杂志,23(17):3394-3395
徐轶肖,王爱辉,胡蓉等,2012.中国南部沿海近岸西加鱼毒素研究.中国环境科学,32(2):330-336
高春蕾,刘仁沿,梁玉波等,2010.虾夷扇贝毒素yessotoxins(YTXs),中国沿海贝类中首次发现的一组贝类生物毒素.海洋学报,32(3):129-137
黄翔,江天久,吴霓,2013a.黄海海域贝类腹泻性贝类毒素污染状况研究.海洋环境科学,32(2):178-181
黄翔,雷芳,江天久,2013b.我国东海和南海近岸海域腹泻性贝类毒素污染状况.暨南大学学报(自然科学版),34(1):101-105
黄海燕,黄爱君,刘建军等,2010.深圳市海域贝类腹泻性毒素的污染状况分析.中国卫生检验杂志,20(3):624-625
梁玉波,2012.中国赤潮灾害调查与评价(1933-2009).北京:海洋出版社,543-659
宿志伟,赵峰,姜雪等,2017.桑沟湾养殖牡蛎中贝类毒素监测及预警.食品科学,38(6):303-309
彭荣飞,李晓晶,于鸿等,2014.广东省经济贝类中多种贝类毒素的监测分析.中国卫生检验杂志,24(21):3129-3131
韩华,周春,于光磊等,2012.大窑湾海域虾夷扇贝体内麻痹性贝毒的周年变化.海洋环境科学,31(3):436-438
傅云娜,陈则玲,2003.腹泻性贝毒的高效液相色谱法测定条件改进及其运用.海洋通报,22(1):92-95
潘柳波,黄薇,王舟等,2016.深圳市市售贝类的腹泻性贝类毒素污染状况分析.职业与健康,32(5):630-632
Anderson D M,Kulis D M,Qi Y Z et al,1996.Paralytic shellfish poisoning in southern China.Toxicon,34(5):579-590
Bricelj V M,Shumway S E,1998.Paralytic shellfish toxins in bivalve molluscs:Occurrence,transfer kinetics,and biotransformation.Reviews in Fisheries Science,6(4):315-383
Chen J H,Han T Z,Li X T et al,2018.Occurrence and distribution of marine natural organic pollutants:Lipophilic marine algal toxins in the Yellow Sea and the Bohai Sea,China.Science of the Total Environment,612:931-939
Chen J H,Li X,Wang S et al,2017.Screening of lipophilic marine toxins in marine aquaculture environment using liquid chromatographye-mass spectrometry.Chemosphere,168:32-40
Chen J H,Yu R C,Gao Y et al,2013.Tracing the origin of paralytic shellfish toxins in scallop Patinopecten yessoensis in the northern Yellow Sea.Food Additives&Contaminants:Part A,30(11):1933-1945
Dai X F,Mak Y L,Lu C K et al,2017.Taxonomic assignment of the benthic toxigenic dinoflagellate Gambierdiscus sp.type6 as Gambierdiscus balechii(Dinophyceae),including its distribution and ciguatoxicity.Harmful Algae,67:107-118
Dickman M,Tang S M,Lai J,2002.A comparison of eastern and western Hong Kong Phytoplankton from weekly samples(1997-1999).Chinese Journal of Oceanology and Limnology,20(1):52-61
Ding L,Qiu J B,Li A F,2017.Proposed biotransformation pathways for new metabolites of paralytic shellfish toxins based on field and experimental mussel samples.Journal of Agricultural and Food Chemistry,65(27):5494-5502
Dorantes-Aranda J J,Campbell K,Bradbury A et al,2017.Comparative performance of four immunological test kits for the detection of Paralytic Shellfish Toxins in Tasmanian shellfish.Toxicon,125:110-119
EU,2011.Commission Regulation No15/2011 of 10 January 2011.Official Journal of the European Union.L6,3-9
EU,European Union Reference Laboratory for Marine Biotoxins(EU-RL-MB),2015.EU-Harmonised Standard Operating Procedure for determination of Lipophilic marine biotoxins in molluscs by LC-MS/MS.Version 5
Fernández M L,Cembella A D,1995.Mammalian bioassays.In:Hallegraeff G M,Anderson D M,Cembella A D eds.Manual on Harmful Marine Microalgae.IOC Manuals and Guides.Paris:UNESCO,33:213-228
Friedman M A,Fernandez M,Backer L C et al,2017.An updated review of ciguatera fish poisoning:clinical,epidemiological,environmental,and public health management.Marine Drugs,15(3):72
Gao H,An X,Liu L et al,2017.Characterization of Dinophysis acuminata from the Yellow Sea,China,and its response to temperature and different Mesodinium prey.Oceanology Hydrobiology Study,4(46):439-450
Gao Y,Yu R C,Chen J H et al,2015a.Distribution of Alexandrium fundyense and A.pacificum(Dinophyceae)in the Yellow Sea and Bohai Sea.Marine Pollution Bulletin,96(1-2):210-219
Gao Y,Yu R C,Murray S A et al,2015b.High specificity of a quantitative PCR assay targeting a saxitoxin gene for monitoring toxic algae associated with paralytic shellfish toxins in the Yellow Sea.Applied Environmental Microbiology,81(20):6973-6981
Genovesi B,Berrebi P,Nagai S et al,2015.Geographic structure evidenced in the toxic dinoflagellate Alexandrium pacificum Litaker(A.catenella-group IV(Whedon&Kofoid)Balech)along Japanese and Chinese coastal waters.Marine Pollution Bulletin,98(1-2):95-105
Gu H F,2011.Morphology,phylogenetic position,and ecophysiology of Alexandrium ostenfeldii(Dinophyceae)from the Bohai Sea,China.Journal of Systematics and Evolution,49(6):606-616
Gu H F,Liu T T,Vale P et al,2013b.Morphology,phylogeny and toxin profiles of Gymnodinium inusitatum sp.nov.,Gymnodinium catenatum and Gymnodinium microreticulatum(Dinophyceae)from the Yellow Sea,China.Harmful Algae,28:97-107
Gu H F,Luo Z H,Krock B et al,2013c.Morphology,phylogeny and azaspiracid profile of Azadinium poporum(Dinophyceae)from the China Sea.Harmful Algae,21-22:64-75
Gu H F,Zeng N,Liu T T et al,2013a.Morphology,toxicity,and phylogeny of Alexandrium(Dinophyceae)species along the coast of China.Harmful Algae,27:68-81
Harrison K,Johnson S,Turner A D,2016.Application of rapid test kits for the determination of paralytic shellfish poisoning(PSP)toxins in bivalve molluscs from Great Britain.Toxicon,119:352-361
Jellett J F,Roberts R L,Laycock M V et al,2002.Detection of paralytic shellfish poisoning(PSP)toxins in shellfish tissue using MIST AlertTM,a new rapid test,in parallel with the regulatory AOAC?mouse bioassay.Toxicon,40(10):1407-1425
Jiang T,Liu L,Li Y et al,2017.Occurrence of marine algal toxins in oyster and phytoplankton samples in Daya Bay,South China Sea.Chemosphere,183:80-88
Jiang T,Xu Y X,Li Y et al,2014.Seasonal dynamics of Alexandrium tamarense and occurrence of paralytic shellfish poisoning toxins in bivalves in Nanji Islands,East China Sea.Marine and Freshwater Research,65(4):350-358
John U,R.Litaker R W,Montresor M et al,2014.Formal revision of the Alexandrium tamarense species complex(Dinophyceae)taxonomy:the introduction of five species with emphasis on molecular-based(rDNA)classification.Protist,165(6):779-804
Krock B,Tillmann U,Witt M et al,2014.Azaspiracid variability of Azadinium poporum(Dinophyceae)from the China Sea.Harmful Algae,36:22-28
LeDoux M,Hall S,2000.Proficiency testing of eight French Laboratories in using the AOAC mouse bioassay for paralytic shellfish poisoning:Interlaboratory collaborative study.Journal of AOAC International,83(2):305-310
Li Z X,Guo M M,Yang S G et al,2010.Investigation of pectenotoxin profiles in the Yellow Sea(China)using a passive sampling technique.Marine Drugs,8(4):1263-1272
Li Y,Huang C X,Xu G S et al,2017b.Pseudo-nitzschia simulans sp.nov.(Bacillariophyceae),the first domoic acid producer from Chinese waters.Harmful Algae,67:119-130
Li A F,Jiang B Z,Chen H D et al,2016.Growth and toxin production of Azadinium poporum strains in batch cultures under different nutrient conditions.Ecotoxicology and Environmental Safety,127:117-126
Li X,Li Z Y,Chen J H et al,2014.Detection,occurrence and monthly variations of typical lipophilic marine toxins associated with diarrhetic shellfish poisoning in the coastal seawater of Qingdao City,China.Chemosphere,111:560-567
Li A,Ma J G,Cao J J et al,2012a.Analysis of paralytic shellfish toxins and their metabolites in shellfish from the North Yellow Sea of China.Food Additives&Contaminants:Part A,29(9):1455-1464
Li A F,Ma J G,Cao J J et al,2012b.Toxins in mussels(Mytilus galloprovincialis)associated with diarrhetic shellfish poisoning episodes in China.Toxicon,60(3):420-425
Li A F,Sun G,Qiu J B et al,2015.Lipophilic shellfish toxins in Dinophysis caudata picked cells and in shellfish from the East China Sea.Environmental Science and Pollution Research,22(4):3116-3126
Li M H,Sun G,Qiu J B et al,2017.Occurrence and variation of lipophilic shellfish toxins in phytoplankton,shellfish and seawater samples from the aquaculture zone in the Yellow Sea,China.Toxicon,127:1-10
Liu R Y,Liang Y B,Wu X L et al,2011.First report on the detection of pectenotoxin groups in Chinese shellfish by LC-MS/MS.Toxicon,57(7-8):1000-1007
Liu R Y,Liu L,Xu Y L et al,2016.The production,distribution and fate of yessotoxins,taking the northern Yellow Sea of China for example.Environment and Ecology Research,4(6):322-345
Liu L,Wei N,Gou Y X et al,2017.Seasonal variability of Protoceratium reticulatum and yessotoxins in Japanese scallop Patinopecten yessoensis in northern Yellow Sea of China.Toxicon,139:31-40
Liu Y,Yu R C,Kong F Z et al,2017a.Paralytic shellfish toxins in phytoplankton and shellfish samples collected from the Bohai Sea,China.Marine Pollution Bulletin,115(1-2):324-331
Liu Y,Yu R C,Kong F Z et al,2017b.Lipophilic marine toxins discovered in the Bohai Sea using high performance liquid chromatography coupled with tandem mass spectrometry.Chemosphere,183:380-388
Luo Z H,Krock B,Mertens K N et al,2017b.Adding new pieces to the Azadinium(Dinophyceae)diversity and biogeography puzzle:non-toxigenic Azadinium zhuanum sp.nov.from China,toxigenic A.poporum from the Mediterranean,and a non-toxigenic A.dalianense from the French Atlantic.Harmful Algae,66:65-78
Luo Z H,Zhang H,Krock B et al,2017a.Morphology,molecular phylogeny and okadaic acid production of epibenthic Prorocentrum(Dinophyceae)species from the northern South China Sea.Algal Research,22:14-30
Mattarozzi M,Milioli M,Bianchi F et al,2016.Optimization of a rapid QuEChERS sample treatment method for HILIC-MS2analysis of paralytic shellfish poisoning(PSP)toxins in Mussels.Food Control,60:138-145
McFarren E F,1959.Report on collaborative studies of the bioassay for paralytic shellfish poison.Journal of the Association of Official Analytical Chemists,42:263-271
Mountfort D,Beuzenberg V,MacKenzie L et al,2006.Enhancement of growth and gymnodimine production by the marine dinoflagellate,Karenia selliformis.Harmful Algae,5(6):658-664
Park D L,Adams W N,Graham S L et al,1986.Variability of mouse bioassay for determination of paralytic shellfish poisoning toxins.Journal of the Association of Official Analytical Chemists,69(3):547-550
Salgado P,RiobóP,Rodríguez F et al,2015.Differences in the toxin profiles of Alexandrium ostenfeldii(Dinophyceae)strains isolated from different geographic origins:Evidence of paralytic toxin,spirolide,and gymnodimine.Toxicon,103:85-98
Selwood A I,Wilkins A L,Munday R et al,2014.Pinnatoxin H:a new pinnatoxin analogue from a South China Sea Vulcanodinium rugosum isolate.Tetrahedron Letters,55(40):5508-5510
Shen H H,Chen J H,Xu X L et al,2018.Development of a high performance liquid chromatography-tandem mass spectrometry method for determination of lipophilic toxins in marine shellfishes and edible safety evaluation.Chinese Journal of Analytical Chemistry,46(6):985-992
Shin C,Jang H,Jo H et al,2017.Development and validation of an accurate and sensitive LC-ESI-MS/MS method for the simultaneous determination of paralytic shellfish poisoning toxins in shellfish and tunicate.Food Control,77:171-178
Turner A D,Goya A B,2016.Comparison of four rapid test kits for the detection of okadaic acid-group toxins in bivalve shellfish from Argentina.Food Control,59:829-840
Usup G,Ahmad A,Matsuoka K et al,2012.Biology,ecology and bloom dynamics of the toxic marine dinoflagellate Pyrodinium bahamense.Harmful Algae,14:301-312
Wang Y L,Chen J H,Li Z Y et al,2015.Determination of typical lipophilic marine toxins in marine sediments from three coastal bays of China using liquid chromatography-tandem mass spectrometry after accelerated solvent extraction.Marine Pollution Bulletin,101(2):954-960
Wang D Z,Hsieh D P H,2005.Growth and toxin production in batch cultures of a marine dinoflagellate Alexandrium tamarense HK9301 isolated from the South China Sea.Harmful Algae,4(2):401-410
Wong C K,Hung P,Lee K L H et al,2009.Effect of steam cooking on distribution of paralytic shellfish toxins in different tissue compartments of scallops Patinopecten yessoensis.Food Chemistry,114(1):72-80
Wong C K,Hung P,Lo J Y C,2014.Ciguatera fish poisoning in Hong Kong-A 10-year perspective on the class of ciguatoxins.Toxicon,86:96-106
Wang D Z,Lin L,Gu H F et al,2008.Comparative studies on morphology,ITS sequence and protein profile of Alexandrium tamarense and A.catenella isolated from the China Sea.Harmful Algae,7(1):106-113
Wang Z H,Nie X P,Jiang S J et al,2011.Source and profile of paralytic shellfish poisoning toxins in shellfish in Daya Bay,South China Sea.Marine Environmental Research,72(1-2):53-59
Wang L,Zhuang Y Y,Zhang H et al,2014.DNA barcoding species in Alexandrium tamarense complex using ITS and proposing designation of five species.Harmful Algae,31:100-113
Wu J Y,Zheng L,Wang J H,2005.Contamination of shellfish from Shanghai seafood markets with paralytic shellfish poisoning and diarrhetic shellfish poisoning toxins determined by mouse bioassay and HPLC.Food Additives&Contaminants,22(7):647-651
Zhang H,Wu Z,Cen J Y et al,2016.First report of three benthic dinoflagellates,Gambierdiscus pacificus,G.australes and G.caribaeus(Dinophyceae),from Hainan Island,South China Sea.Phycological Research,64(4):259-273
Zhuo L Y,Yin Y C,Fu W S et al,2013.Determination of paralytic shellfish poisoning toxins by HILIC-MS/MScoupled with dispersive solid phase extraction.Food Chemistry,137(1-4):115-121
Zhou M J,Li J,Luckas B et al,1999.A recent shellfish toxin investigation in China.Marine Pollution Bulletin,39(1-12):331-334
Zou C,Ye R M,Zheng J W et al,2014.Molecular phylogeny and PSP toxin profile of the Alexandrium tamarense species complex along the coast of China.Marine Pollution Bulletin,89(1-2):209-219
王舟,黄薇,潘柳波等,2012.2011年深圳市水产品卫生状况及风险评估.中国公共卫生管理,28(5):595-597
王茜,程金平,高利利等,2012.软骨藻酸直接竞争ELISA方法的建立及优化.环境科学,33(2):647-651
王恒,2011.舟山海域贝类海产品中软骨藻酸含量调查.中国卫生检验杂志,21(12):2986-2988,2992
王金辉,秦玉涛,刘材材等,2007.长江口及邻近海域有毒藻类和赤潮毒素的本底调查.海洋湖沼通报,(1):52-61
王雪虹,黄世玉,2007.厦门海域养殖区麻痹性贝毒污染的研究.集美大学学报(自然科学版),12(2):114-117
勾玉晓,刘磊,李冬梅等,2018.北黄海慢原甲藻形态结构与腹泻性贝类毒素组成.中国渔业质量与标准,8(3):11-18
孔凡洲,徐子钧,于仁成等,2007.黄渤海海域贝类麻痹性贝毒的检测与分析.中国海洋大学学报,37(2):305-309
母清林,王晓华,佘运勇等,2013.浙江近岸海域贝类中重金属和贝毒污染状况研究.海洋科学,37(1):87-91
吉薇,郑洁莹,曾雪萍等,2011.南海海域软骨藻酸(DA)贝类毒素的HPLC方法检测.现代食品科技,27(1):120-122,116
刘丽,刘磊,赵芮等,2016.酶联免疫吸附分析检测鳍藻毒素DTX1和DTX2.分析科学学报,32(4):557-560
刘俏,龙丽娟,2013.南海慢原甲藻的培养和毒性初探.湖北农业科学,52(5):1113-1117
刘仁沿,许道艳,董玉华等,2009.海水和贝类中软骨藻酸的酶联免疫吸附分析方法研究.卫生研究,38(5):662-624
刘仁沿,陈冰君,梁玉波等,2008a.腹泻性贝毒软海绵酸单克隆抗体的制备和酶联免疫检测方法的建立.卫生研究,37(4):443-445
刘仁沿,高春蕾,梁玉波等,2008c.Gymnodimine,首次在我国北海缘齿牡蛎中发现的一种腹泻性贝毒组分.海洋学报,30(6):171-176
刘仁沿,梁玉波,刘磊等,2014.液相色谱结合串联质谱方法研究中国沿海贝类中脂溶性藻毒素的种类结构和分布规律.生态环境学报,23(8):1320-1326
刘仁沿,梁玉波,陈冰君等,2008b.胶体金免疫层析方法快速检测腹泻性贝毒软海绵酸的初步研究.分析试验室,27(7):26-29
刘淑娟,赵晓祥,程金平等,2014.快速检测软骨藻酸的间接ELISA方法.环境科学学报,34(2):404-408
关春江,冯志权,马明辉等,1999.长江以北沿海经济贝类中的麻痹性贝毒.海洋环境科学,18(2):49-52
江天久,尹伊伟,骆育敏等,2000b.广东大亚湾和大鹏湾麻痹性贝类毒素研究.中国环境科学,20(4):341-344
江天久,尹伊伟,黄伟建等,2000a.深圳大亚湾麻痹性贝类毒素成分特征.暨南大学学报(自然科学版),21(5):65-69
江天久,包财,雷芳等,2010.广东东部沿海麻痹性贝类毒素成分特征分析.中国水产科学,17(1):119-127
江天久,江涛,2007.中国沿海部分海域麻痹性贝毒研究.海洋与湖沼,38(1):36-41
江天久,陈菊芳,邹迎麟等,2003.中国东海和南海有害赤潮高发区麻痹性贝毒素研究.应用生态学报,14(7):1156-1160
许道艳,刘磊,刘仁沿等,2013.麻痹性贝毒单克隆抗体的制备和酶联免疫检测方法的建立.中国免疫学杂志,29(1):69-73
许道艳,刘仁沿,董玉华等,2007.失去记忆性贝毒ASP酶联免疫检测方法的研究.海洋环境科学,26(3):237-240
孙烨,刘威,郑剑等,2016.2011-2014年深圳市贝类产品生物毒素污染状况调查.检测研究,28(2):149-150,154
杜克梅,江天久,吴霓,2013a.黄海海域贝类麻痹性贝类毒素污染状况研究.海洋环境科学,32(2):182-184
杜克梅,雷芳,吴霓等,2013b.我国东海和南海近岸海域麻痹性贝类毒素污染状况.暨南大学学报(自然科学版),34(3):343-346
李大志,祝文君,朱文斌等,2002.记忆缺失性贝类毒素的主要成分--软骨藻酸的毛细管电泳分析.色谱,20(2):125-128
李伟才,栾刚,李立等,2000.中国沿海部分海区贝毒毒素的调查.海洋科学,24(9):19-23
李晓晶,彭荣飞,于鸿等,2013.广州市市售水产品中多种脂溶性贝类毒素的监测.中国卫生检验杂志,23(10):2317-2319
李嘉雯,江涛,吴锋等,2014.珠江口与大亚湾海域腹泻性贝毒污染状况分析.暨南大学学报(自然科学与医学版),35(3):228-234
杨莉,杨维东,刘洁生等,2006.广州市售贝类麻痹性贝毒和腹泻性贝毒污染状况分析.卫生研究,35(4):435-439
杨美兰,林钦,吕晓瑜等,2005.广东重要渔业水域牡蛎体中的麻痹性贝类毒素.海洋环境科学,24(1):48-50
杨美兰,林燕棠,全桂英,1999.广东沿海牡蛎体的麻痹性毒素与评价.湛江海洋大学学报,19(3):38-42
杨美兰,林燕棠,贾晓平等,2002.珠江口及邻近海域贝类麻痹性毒素调查.中国水产科学,9(3):283-285
杨美兰,贾晓平,林钦等,2009.南海海域重要养殖水域牡蛎体中的腹泻性贝类毒素.海洋环境科学,28(4):410-413
吴锋,江天久,张帆等,2010.浙江南麂列岛海域贝类中的麻痹性贝类毒素研究.海洋环境科学,29(3):360-363
吴施卫,卢楚谦,朱小山等,2005a.广东沿海麻痹性贝毒素的地理分布特征.海洋环境科学,24(3):40-43
吴施卫,张纯超,卢楚谦等,2005b.南海近岸海域腹泻性贝类毒素分析.海洋环境科学,24(4):48-51
吴施卫,曾淼,卢大鹏等,2008a.广东近岸海域2005年春季的腹泻性贝毒素特征分析.海洋环境科学,27(2):165-168,181
吴施卫,曾淼,卢大鹏等,2008b.2005年春季广东近岸海域麻痹性贝毒素的特征分析.海洋学研究,26(4):49-54
何依娜,金雷,梅光明等,2016.舟山市海水养殖贝类质量安全现状评价.安徽农业科学,44(27):122-123,203
冷科明,吴霓,杜克梅等,2013.粤西海域麻痹性贝类毒素成分特征分析.生态科学,32(5):558-563
冷科明,吴霓,杜克梅等,2014.粤中海域麻痹性贝类毒素成分特征分析.海洋环境科学,33(5):666-671
宋向明,谢小华,赵冲厚等,2013.酶联免疫法测定牡蛎中腹泻性毒素的研究.水产养殖,34(1):49-52
宋琍琍,张海琪,侯镜德等,2008.液相色谱-串联质谱法测定贝类毒素软骨藻酸的残留.水产学报,32(6):950-956
宋普江,张伟,王刚等,2011.大连近岸海域经济贝类麻痹性贝毒分析.海洋环境科学,30(4):533-535,545
宋新成,周德山,尹华斌等,2016.海州湾近海海域海水中贝类毒素的检测与季节变化.淮海工学院学报(自然科学版),25(3):83-87
张秀尧,蔡欣欣,2012.从暴发腹泻的贻贝中同时检出腹泻性贝类毒素和扇贝毒素.中国卫生检验杂志,22(8):1905-1907,1911
张树刚,邹清,陈雷等,2011.浙南海域腹泻性贝毒分析.海洋科学,35(1):44-47,53
陈西平,王成斌,胡俊明等,2001.HPLC方法检测水及水生动物中软骨藻酸.卫生研究,30(4):247-248
陈建华,于仁成,孔凡洲等,2014.北黄海海域虾夷扇贝体内脂溶性藻毒素分析.海洋与湖沼,45(4):855-863
林祥田,张明生,王志坚等,2005.连云港海州湾麻痹性贝类毒素中毒分析.中国食品卫生杂志,17(3):243-246
林燕棠,杨美兰,陈瑞雯等,1994.广东沿海麻痹性贝类毒素的研究.海洋与湖沼,25(2):220-225
林燕棠,贾晓平,杨美兰等,1999.中国沿岸染毒贝类的麻痹性毒素.热带海洋,18(1):90-96
林燕棠,贾晓平,杨美兰等,2001.我国海产贝类体中的麻痹性毒素及其来源.水产学报,25(5):479-481
罗璇,于仁成,周名江,2014.应用LC-MS联用方法分析青岛近海渐尖鳍藻(Dinophysis acuminata)细胞中的毒素成分.海洋环境科学,33(5):781-787
郑仁锦,李榕珊,黄宏南等,2016.福建省市售水产品中河豚毒素及腹泻性贝类毒素污染状况.职业与健康,32(17):2367-2370
郑重莺,张海琪,宋琍琍等,2012.浙江省市售主要食用贝类中麻痹性贝类毒素和腹泻性贝类毒素污染状况分析.浙江农业科学,(2):236-239
赵芮,刘磊,刘丽等,2015.酶联免疫吸附法和液相色谱-质谱联用法分析海洋生物中记忆缺失性贝毒.分析试验室,34(8):882-885
胡蓉,江天久,2013.海南岛近岸海域贝类中的腹泻性贝类毒素.暨南大学学报(自然科学版),34(5):527-532
胡颢琰,唐静亮,王益鸣等,2006.浙江近岸有害赤潮发生区麻痹性贝毒素研究.海洋环境科学,25(1):63-65
姚建华,谭志军,周德庆等,2010.液相色谱-串联质谱法检测贝类产品中的原多甲藻酸贝类毒素.色谱,28(4):363-367
夏远征,王双双,辛丘岩等,2010.大连海域贝类麻痹性贝毒的污染状况调查与分析.食品与机械,26(2):54-56
徐奋奋,吴蓓莉,黄亚琴等,2013.宁波市市售海水产品腹泻性贝类毒素检测结果分析.中国卫生检验杂志,23(17):3394-3395
徐轶肖,王爱辉,胡蓉等,2012.中国南部沿海近岸西加鱼毒素研究.中国环境科学,32(2):330-336
高春蕾,刘仁沿,梁玉波等,2010.虾夷扇贝毒素yessotoxins(YTXs),中国沿海贝类中首次发现的一组贝类生物毒素.海洋学报,32(3):129-137
黄翔,江天久,吴霓,2013a.黄海海域贝类腹泻性贝类毒素污染状况研究.海洋环境科学,32(2):178-181
黄翔,雷芳,江天久,2013b.我国东海和南海近岸海域腹泻性贝类毒素污染状况.暨南大学学报(自然科学版),34(1):101-105
黄海燕,黄爱君,刘建军等,2010.深圳市海域贝类腹泻性毒素的污染状况分析.中国卫生检验杂志,20(3):624-625
梁玉波,2012.中国赤潮灾害调查与评价(1933-2009).北京:海洋出版社,543-659
宿志伟,赵峰,姜雪等,2017.桑沟湾养殖牡蛎中贝类毒素监测及预警.食品科学,38(6):303-309
彭荣飞,李晓晶,于鸿等,2014.广东省经济贝类中多种贝类毒素的监测分析.中国卫生检验杂志,24(21):3129-3131
韩华,周春,于光磊等,2012.大窑湾海域虾夷扇贝体内麻痹性贝毒的周年变化.海洋环境科学,31(3):436-438
傅云娜,陈则玲,2003.腹泻性贝毒的高效液相色谱法测定条件改进及其运用.海洋通报,22(1):92-95
潘柳波,黄薇,王舟等,2016.深圳市市售贝类的腹泻性贝类毒素污染状况分析.职业与健康,32(5):630-632
Anderson D M,Kulis D M,Qi Y Z et al,1996.Paralytic shellfish poisoning in southern China.Toxicon,34(5):579-590
Bricelj V M,Shumway S E,1998.Paralytic shellfish toxins in bivalve molluscs:Occurrence,transfer kinetics,and biotransformation.Reviews in Fisheries Science,6(4):315-383
Chen J H,Han T Z,Li X T et al,2018.Occurrence and distribution of marine natural organic pollutants:Lipophilic marine algal toxins in the Yellow Sea and the Bohai Sea,China.Science of the Total Environment,612:931-939
Chen J H,Li X,Wang S et al,2017.Screening of lipophilic marine toxins in marine aquaculture environment using liquid chromatographye-mass spectrometry.Chemosphere,168:32-40
Chen J H,Yu R C,Gao Y et al,2013.Tracing the origin of paralytic shellfish toxins in scallop Patinopecten yessoensis in the northern Yellow Sea.Food Additives&Contaminants:Part A,30(11):1933-1945
Dai X F,Mak Y L,Lu C K et al,2017.Taxonomic assignment of the benthic toxigenic dinoflagellate Gambierdiscus sp.type6 as Gambierdiscus balechii(Dinophyceae),including its distribution and ciguatoxicity.Harmful Algae,67:107-118
Dickman M,Tang S M,Lai J,2002.A comparison of eastern and western Hong Kong Phytoplankton from weekly samples(1997-1999).Chinese Journal of Oceanology and Limnology,20(1):52-61
Ding L,Qiu J B,Li A F,2017.Proposed biotransformation pathways for new metabolites of paralytic shellfish toxins based on field and experimental mussel samples.Journal of Agricultural and Food Chemistry,65(27):5494-5502
Dorantes-Aranda J J,Campbell K,Bradbury A et al,2017.Comparative performance of four immunological test kits for the detection of Paralytic Shellfish Toxins in Tasmanian shellfish.Toxicon,125:110-119
EU,2011.Commission Regulation No15/2011 of 10 January 2011.Official Journal of the European Union.L6,3-9
EU,European Union Reference Laboratory for Marine Biotoxins(EU-RL-MB),2015.EU-Harmonised Standard Operating Procedure for determination of Lipophilic marine biotoxins in molluscs by LC-MS/MS.Version 5
Fernández M L,Cembella A D,1995.Mammalian bioassays.In:Hallegraeff G M,Anderson D M,Cembella A D eds.Manual on Harmful Marine Microalgae.IOC Manuals and Guides.Paris:UNESCO,33:213-228
Friedman M A,Fernandez M,Backer L C et al,2017.An updated review of ciguatera fish poisoning:clinical,epidemiological,environmental,and public health management.Marine Drugs,15(3):72
Gao H,An X,Liu L et al,2017.Characterization of Dinophysis acuminata from the Yellow Sea,China,and its response to temperature and different Mesodinium prey.Oceanology Hydrobiology Study,4(46):439-450
Gao Y,Yu R C,Chen J H et al,2015a.Distribution of Alexandrium fundyense and A.pacificum(Dinophyceae)in the Yellow Sea and Bohai Sea.Marine Pollution Bulletin,96(1-2):210-219
Gao Y,Yu R C,Murray S A et al,2015b.High specificity of a quantitative PCR assay targeting a saxitoxin gene for monitoring toxic algae associated with paralytic shellfish toxins in the Yellow Sea.Applied Environmental Microbiology,81(20):6973-6981
Genovesi B,Berrebi P,Nagai S et al,2015.Geographic structure evidenced in the toxic dinoflagellate Alexandrium pacificum Litaker(A.catenella-group IV(Whedon&Kofoid)Balech)along Japanese and Chinese coastal waters.Marine Pollution Bulletin,98(1-2):95-105
Gu H F,2011.Morphology,phylogenetic position,and ecophysiology of Alexandrium ostenfeldii(Dinophyceae)from the Bohai Sea,China.Journal of Systematics and Evolution,49(6):606-616
Gu H F,Liu T T,Vale P et al,2013b.Morphology,phylogeny and toxin profiles of Gymnodinium inusitatum sp.nov.,Gymnodinium catenatum and Gymnodinium microreticulatum(Dinophyceae)from the Yellow Sea,China.Harmful Algae,28:97-107
Gu H F,Luo Z H,Krock B et al,2013c.Morphology,phylogeny and azaspiracid profile of Azadinium poporum(Dinophyceae)from the China Sea.Harmful Algae,21-22:64-75
Gu H F,Zeng N,Liu T T et al,2013a.Morphology,toxicity,and phylogeny of Alexandrium(Dinophyceae)species along the coast of China.Harmful Algae,27:68-81
Harrison K,Johnson S,Turner A D,2016.Application of rapid test kits for the determination of paralytic shellfish poisoning(PSP)toxins in bivalve molluscs from Great Britain.Toxicon,119:352-361
Jellett J F,Roberts R L,Laycock M V et al,2002.Detection of paralytic shellfish poisoning(PSP)toxins in shellfish tissue using MIST AlertTM,a new rapid test,in parallel with the regulatory AOAC?mouse bioassay.Toxicon,40(10):1407-1425
Jiang T,Liu L,Li Y et al,2017.Occurrence of marine algal toxins in oyster and phytoplankton samples in Daya Bay,South China Sea.Chemosphere,183:80-88
Jiang T,Xu Y X,Li Y et al,2014.Seasonal dynamics of Alexandrium tamarense and occurrence of paralytic shellfish poisoning toxins in bivalves in Nanji Islands,East China Sea.Marine and Freshwater Research,65(4):350-358
John U,R.Litaker R W,Montresor M et al,2014.Formal revision of the Alexandrium tamarense species complex(Dinophyceae)taxonomy:the introduction of five species with emphasis on molecular-based(rDNA)classification.Protist,165(6):779-804
Krock B,Tillmann U,Witt M et al,2014.Azaspiracid variability of Azadinium poporum(Dinophyceae)from the China Sea.Harmful Algae,36:22-28
LeDoux M,Hall S,2000.Proficiency testing of eight French Laboratories in using the AOAC mouse bioassay for paralytic shellfish poisoning:Interlaboratory collaborative study.Journal of AOAC International,83(2):305-310
Li Z X,Guo M M,Yang S G et al,2010.Investigation of pectenotoxin profiles in the Yellow Sea(China)using a passive sampling technique.Marine Drugs,8(4):1263-1272
Li Y,Huang C X,Xu G S et al,2017b.Pseudo-nitzschia simulans sp.nov.(Bacillariophyceae),the first domoic acid producer from Chinese waters.Harmful Algae,67:119-130
Li A F,Jiang B Z,Chen H D et al,2016.Growth and toxin production of Azadinium poporum strains in batch cultures under different nutrient conditions.Ecotoxicology and Environmental Safety,127:117-126
Li X,Li Z Y,Chen J H et al,2014.Detection,occurrence and monthly variations of typical lipophilic marine toxins associated with diarrhetic shellfish poisoning in the coastal seawater of Qingdao City,China.Chemosphere,111:560-567
Li A,Ma J G,Cao J J et al,2012a.Analysis of paralytic shellfish toxins and their metabolites in shellfish from the North Yellow Sea of China.Food Additives&Contaminants:Part A,29(9):1455-1464
Li A F,Ma J G,Cao J J et al,2012b.Toxins in mussels(Mytilus galloprovincialis)associated with diarrhetic shellfish poisoning episodes in China.Toxicon,60(3):420-425
Li A F,Sun G,Qiu J B et al,2015.Lipophilic shellfish toxins in Dinophysis caudata picked cells and in shellfish from the East China Sea.Environmental Science and Pollution Research,22(4):3116-3126
Li M H,Sun G,Qiu J B et al,2017.Occurrence and variation of lipophilic shellfish toxins in phytoplankton,shellfish and seawater samples from the aquaculture zone in the Yellow Sea,China.Toxicon,127:1-10
Liu R Y,Liang Y B,Wu X L et al,2011.First report on the detection of pectenotoxin groups in Chinese shellfish by LC-MS/MS.Toxicon,57(7-8):1000-1007
Liu R Y,Liu L,Xu Y L et al,2016.The production,distribution and fate of yessotoxins,taking the northern Yellow Sea of China for example.Environment and Ecology Research,4(6):322-345
Liu L,Wei N,Gou Y X et al,2017.Seasonal variability of Protoceratium reticulatum and yessotoxins in Japanese scallop Patinopecten yessoensis in northern Yellow Sea of China.Toxicon,139:31-40
Liu Y,Yu R C,Kong F Z et al,2017a.Paralytic shellfish toxins in phytoplankton and shellfish samples collected from the Bohai Sea,China.Marine Pollution Bulletin,115(1-2):324-331
Liu Y,Yu R C,Kong F Z et al,2017b.Lipophilic marine toxins discovered in the Bohai Sea using high performance liquid chromatography coupled with tandem mass spectrometry.Chemosphere,183:380-388
Luo Z H,Krock B,Mertens K N et al,2017b.Adding new pieces to the Azadinium(Dinophyceae)diversity and biogeography puzzle:non-toxigenic Azadinium zhuanum sp.nov.from China,toxigenic A.poporum from the Mediterranean,and a non-toxigenic A.dalianense from the French Atlantic.Harmful Algae,66:65-78
Luo Z H,Zhang H,Krock B et al,2017a.Morphology,molecular phylogeny and okadaic acid production of epibenthic Prorocentrum(Dinophyceae)species from the northern South China Sea.Algal Research,22:14-30
Mattarozzi M,Milioli M,Bianchi F et al,2016.Optimization of a rapid QuEChERS sample treatment method for HILIC-MS2analysis of paralytic shellfish poisoning(PSP)toxins in Mussels.Food Control,60:138-145
McFarren E F,1959.Report on collaborative studies of the bioassay for paralytic shellfish poison.Journal of the Association of Official Analytical Chemists,42:263-271
Mountfort D,Beuzenberg V,MacKenzie L et al,2006.Enhancement of growth and gymnodimine production by the marine dinoflagellate,Karenia selliformis.Harmful Algae,5(6):658-664
Park D L,Adams W N,Graham S L et al,1986.Variability of mouse bioassay for determination of paralytic shellfish poisoning toxins.Journal of the Association of Official Analytical Chemists,69(3):547-550
Salgado P,RiobóP,Rodríguez F et al,2015.Differences in the toxin profiles of Alexandrium ostenfeldii(Dinophyceae)strains isolated from different geographic origins:Evidence of paralytic toxin,spirolide,and gymnodimine.Toxicon,103:85-98
Selwood A I,Wilkins A L,Munday R et al,2014.Pinnatoxin H:a new pinnatoxin analogue from a South China Sea Vulcanodinium rugosum isolate.Tetrahedron Letters,55(40):5508-5510
Shen H H,Chen J H,Xu X L et al,2018.Development of a high performance liquid chromatography-tandem mass spectrometry method for determination of lipophilic toxins in marine shellfishes and edible safety evaluation.Chinese Journal of Analytical Chemistry,46(6):985-992
Shin C,Jang H,Jo H et al,2017.Development and validation of an accurate and sensitive LC-ESI-MS/MS method for the simultaneous determination of paralytic shellfish poisoning toxins in shellfish and tunicate.Food Control,77:171-178
Turner A D,Goya A B,2016.Comparison of four rapid test kits for the detection of okadaic acid-group toxins in bivalve shellfish from Argentina.Food Control,59:829-840
Usup G,Ahmad A,Matsuoka K et al,2012.Biology,ecology and bloom dynamics of the toxic marine dinoflagellate Pyrodinium bahamense.Harmful Algae,14:301-312
Wang Y L,Chen J H,Li Z Y et al,2015.Determination of typical lipophilic marine toxins in marine sediments from three coastal bays of China using liquid chromatography-tandem mass spectrometry after accelerated solvent extraction.Marine Pollution Bulletin,101(2):954-960
Wang D Z,Hsieh D P H,2005.Growth and toxin production in batch cultures of a marine dinoflagellate Alexandrium tamarense HK9301 isolated from the South China Sea.Harmful Algae,4(2):401-410
Wong C K,Hung P,Lee K L H et al,2009.Effect of steam cooking on distribution of paralytic shellfish toxins in different tissue compartments of scallops Patinopecten yessoensis.Food Chemistry,114(1):72-80
Wong C K,Hung P,Lo J Y C,2014.Ciguatera fish poisoning in Hong Kong-A 10-year perspective on the class of ciguatoxins.Toxicon,86:96-106
Wang D Z,Lin L,Gu H F et al,2008.Comparative studies on morphology,ITS sequence and protein profile of Alexandrium tamarense and A.catenella isolated from the China Sea.Harmful Algae,7(1):106-113
Wang Z H,Nie X P,Jiang S J et al,2011.Source and profile of paralytic shellfish poisoning toxins in shellfish in Daya Bay,South China Sea.Marine Environmental Research,72(1-2):53-59
Wang L,Zhuang Y Y,Zhang H et al,2014.DNA barcoding species in Alexandrium tamarense complex using ITS and proposing designation of five species.Harmful Algae,31:100-113
Wu J Y,Zheng L,Wang J H,2005.Contamination of shellfish from Shanghai seafood markets with paralytic shellfish poisoning and diarrhetic shellfish poisoning toxins determined by mouse bioassay and HPLC.Food Additives&Contaminants,22(7):647-651
Zhang H,Wu Z,Cen J Y et al,2016.First report of three benthic dinoflagellates,Gambierdiscus pacificus,G.australes and G.caribaeus(Dinophyceae),from Hainan Island,South China Sea.Phycological Research,64(4):259-273
Zhuo L Y,Yin Y C,Fu W S et al,2013.Determination of paralytic shellfish poisoning toxins by HILIC-MS/MScoupled with dispersive solid phase extraction.Food Chemistry,137(1-4):115-121
Zhou M J,Li J,Luckas B et al,1999.A recent shellfish toxin investigation in China.Marine Pollution Bulletin,39(1-12):331-334
Zou C,Ye R M,Zheng J W et al,2014.Molecular phylogeny and PSP toxin profile of the Alexandrium tamarense species complex along the coast of China.Marine Pollution Bulletin,89(1-2):209-219