一测多评法同时测定复生康胶囊中8个成分的含量
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摘要
目的:采用一测多评法同时测定复生康胶囊中羟喜树碱、喜树碱、荭草苷、异荭草苷、牡荆苷、异牡荆苷、莪术二酮和莪术醇的含量。方法:色谱柱为Hypersil ODS柱(4.6 mm×250 mm,5μm);以甲醇-乙腈(2∶1)与0.05%冰醋酸溶液为流动相,梯度洗脱;柱温为25℃;检测波长分别为254 nm(检测羟喜树碱和喜树碱)、340 nm(检测荭草苷、异荭草苷、牡荆苷和异牡荆苷)、214 nm(检测莪术二酮和莪术醇);体积流量为0.9 mL·min~(-1);以牡荆苷为内标物,建立牡荆苷与羟喜树碱、喜树碱、荭草苷、异荭草苷、异牡荆苷、莪术二酮和莪术醇的相对校正因子,测定其含量。结果:羟喜树碱、喜树碱、荭草苷、异荭草苷、牡荆苷、异牡荆苷、莪术二酮和莪术醇分别在2.69～67.25、3.88～97.00、1.46～36.50、1.77～44.25、1.19～29.75、1.85～46.25、2.09～52.25、1.73～43.25μg·mL~(-1)范围内线性关系良好;平均加样回收率(RSD)分别为99.1%(0.91%)、100.1%(0.67%)、97.9%(1.2%)、98.2%(1.4%)、97.5%(0.97%)、97.0%(1.4%)、98.9%(0.89%)和98.1%(1.3%);一测多评法所得结果与外标法无显著差异。结论:一测多评法可以应用于复生康胶囊的多指标质量评价。
Objective:To establish a method for simultaneous determination of hydroxycamptothecin, camptothecine,orientin,isoorientin,vitexin,isovitexin,curdione and curcumol in Fushengkang capsule by multi-components by single-marker(QAMS) method.Methods:The analysis was performed on a Hypersil ODS column(4.6 mm×250 mm,5 μm),with the mobile phase comprising of methanol-acetonitrile(2∶1)(A)-0.05% glacial aceticacid solution(B) flowing at 0.9 mL·min~(-1) in a gradient elution.The column temperature was set at 25 ℃,and the detection wavelengths were set at 254 nm(hydroxycamptothecin and camptothecine),340 nm(orientin, isoorientin,vitexin and isovitexin) and 214 nm(curdione and curcumol).With vitexin as the internal standard, the relative correction factors of hydroxycamptothecin,camptothecine,orientin, isoorientin,isovitexin,curdione and curcumol were calculated, based on which the contents were determined.Results:The standard curves of hydroxycamptothecin,camptothecine,orientin, isoorientin,vitexin,isovitexin,curdione and curcumol showed good linearity in 2.69-67.25,3.88-97.00,1.46-36.50,1.77-44.25,1.19-29.75,1.85-46.25,2.09-52.25 and1.73-43.25 μg·mL~(-1),respectively.The average recoveries and the corresponding RSD were 99.13%(0.91%),100.1%(0.67%),97.9%(1.2%),98.2%(1.4%),97.5%(0.97%),97.0%(1.4%),98.9%(0.89%) and 98.1%(1.3%),respectively.The results obtained by QAMS approximated with those obtained by external standard method.Conclusion:The QAMS method can be used to the multi-index quality evaluation of Fushengkang capsule.
Objective:To establish a method for simultaneous determination of hydroxycamptothecin, camptothecine,orientin,isoorientin,vitexin,isovitexin,curdione and curcumol in Fushengkang capsule by multi-components by single-marker(QAMS) method.Methods:The analysis was performed on a Hypersil ODS column(4.6 mm×250 mm,5 μm),with the mobile phase comprising of methanol-acetonitrile(2∶1)(A)-0.05% glacial aceticacid solution(B) flowing at 0.9 mL·min~(-1) in a gradient elution.The column temperature was set at 25 ℃,and the detection wavelengths were set at 254 nm(hydroxycamptothecin and camptothecine),340 nm(orientin, isoorientin,vitexin and isovitexin) and 214 nm(curdione and curcumol).With vitexin as the internal standard, the relative correction factors of hydroxycamptothecin,camptothecine,orientin, isoorientin,isovitexin,curdione and curcumol were calculated, based on which the contents were determined.Results:The standard curves of hydroxycamptothecin,camptothecine,orientin, isoorientin,vitexin,isovitexin,curdione and curcumol showed good linearity in 2.69-67.25,3.88-97.00,1.46-36.50,1.77-44.25,1.19-29.75,1.85-46.25,2.09-52.25 and1.73-43.25 μg·mL~(-1),respectively.The average recoveries and the corresponding RSD were 99.13%(0.91%),100.1%(0.67%),97.9%(1.2%),98.2%(1.4%),97.5%(0.97%),97.0%(1.4%),98.9%(0.89%) and 98.1%(1.3%),respectively.The results obtained by QAMS approximated with those obtained by external standard method.Conclusion:The QAMS method can be used to the multi-index quality evaluation of Fushengkang capsule.
引文
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[8]隆林,罗朝凤,李天先,等.喜树果质量标准修定研究[J].现代食品,2017,3(15):87LONG L,LUO CF,LI TX,et al.Study on quality standard revision of Camptotheca Acuminata Decne[J].Mod Food,2017,3(15):87
[9]陈艳,姚宏,李少光,等.HPLC法测定蒲葵药材中6种组分含量[J].药物分析杂志,2011,31(4):645CHEN Y,YAO H,LI SG,et al.HPLC determination of the six components in Livistona Chinensis[J].Chin J Pharm Anal,2011,31(4):645
[10]毛春芹,陆兔林,姜国非,等.HPLC法测定不同主产地莪术饮片中莪术二酮,莪术醇,吉马酮和β-榄香烯[J].中草药,2013,44(3):305MAO CQ,LU TL,JIANG GF,et al.Determination of curdione,curcumol,germacrone,andβ-elemene in processed Curcumae Rhizoma from different habitats[J].Chin Tradit Herb Drugs,2013,44(3):305
[11]姜国非,陆兔林,毛春芹,等.不同产地醋莪术饮片中莪术二酮,莪术醇,吉马酮和β-榄香烯含量的比较[J].中国中药杂志,2010,35(21):2834JIANG GF,LU TL,MAO CQ,et al.Comparison of content of curdione,curcumol,germacrone andβ-elemene in different varieties of vinegar backed Rhizoma Curcuma[J].China J Chin Mater Med,2010,35(21):2834
[12]何欢,马双成,田颂九,等.HPLC测定莪术油及其注射液中6种成分的含量[J].中国中药杂志,2010,35(5):593HE H,MA SC,TIAN SJ,et al.Simultaneous determination by HPLC of 6 components in zedoary turmeric oil and its related injections with replacement method of chemical reference substance[J].China J Chin Mater Med,2010,35(5):593
[2]黄瑞松,叶云峰,苏青,等.复生康胶囊质量标准的研究[J].中草药,2003,34(8):698HUANG RS,YE YF,SU Q,et al.Studies on quality standard of Fushengkang capsules[J].Chin Tradit Herb Drugs,2003,34(8):698
[3]颜栋林,李萍,兰茜.复生康胶囊微生物限度检查法方法验证[J].长春中医药大学学报,2010,26(1):131YAN DL,LI P,LAN Q.Microbial limit test method verification of Fushengkang capsule[J].J Changchun Univ Tradit Chin Med,2010,26(1):131
[4]逄瑜,孙磊,金红宇,等.替代对照品法在中药多指标含量测定中的应用与技术要求探讨[J].药物分析杂志,2013,33(1):169PANG Y,SUN L,JIN HY,et al.Discussion on application and technical requirements of substitute reference substance method for simultaneous determination of multi-components in traditional Chinese medicine[J].Chin J Pharm Anal,2013,33(1):169
[5]文乾映,龙芳,杨华,等.中药质量控制中一测多评法的应用进展[J].中国药房,2014,25(23):2185WEN QY,LONG F,YANG H,et al.Advance in quality control for traditional Chinese medicines by QAMS[J].China Pharm,2014,25(23):2185
[6]黄晓舞,白林,张莉.HPLC法测定喜树果中喜树碱和羟基喜树碱的含量[J].科学技术与工程,2005,5(12):809HUANG XW,BAI L,ZHANG L.HPLC determination of camptothecine and 10-hydroxycamptothecine in Common Camptotheca fruit[J].Sci Technol Engin,2005,5(12):809
[7]刘文哲,秦海燕,索志荣.喜树果中喜树碱和10-轻基喜树碱的HPLC分析[J].药物分析杂志,2005,25(2):168LIU WZ,QING HY,SUO ZR.HPLC analysis of camptothecine and10-hydroxycamptothecine in fruit of Camptotheca Acuminata Decne[J].Chin J Pharm Anal,2005,25(2):168
[8]隆林,罗朝凤,李天先,等.喜树果质量标准修定研究[J].现代食品,2017,3(15):87LONG L,LUO CF,LI TX,et al.Study on quality standard revision of Camptotheca Acuminata Decne[J].Mod Food,2017,3(15):87
[9]陈艳,姚宏,李少光,等.HPLC法测定蒲葵药材中6种组分含量[J].药物分析杂志,2011,31(4):645CHEN Y,YAO H,LI SG,et al.HPLC determination of the six components in Livistona Chinensis[J].Chin J Pharm Anal,2011,31(4):645
[10]毛春芹,陆兔林,姜国非,等.HPLC法测定不同主产地莪术饮片中莪术二酮,莪术醇,吉马酮和β-榄香烯[J].中草药,2013,44(3):305MAO CQ,LU TL,JIANG GF,et al.Determination of curdione,curcumol,germacrone,andβ-elemene in processed Curcumae Rhizoma from different habitats[J].Chin Tradit Herb Drugs,2013,44(3):305
[11]姜国非,陆兔林,毛春芹,等.不同产地醋莪术饮片中莪术二酮,莪术醇,吉马酮和β-榄香烯含量的比较[J].中国中药杂志,2010,35(21):2834JIANG GF,LU TL,MAO CQ,et al.Comparison of content of curdione,curcumol,germacrone andβ-elemene in different varieties of vinegar backed Rhizoma Curcuma[J].China J Chin Mater Med,2010,35(21):2834
[12]何欢,马双成,田颂九,等.HPLC测定莪术油及其注射液中6种成分的含量[J].中国中药杂志,2010,35(5):593HE H,MA SC,TIAN SJ,et al.Simultaneous determination by HPLC of 6 components in zedoary turmeric oil and its related injections with replacement method of chemical reference substance[J].China J Chin Mater Med,2010,35(5):593