山楂叶与三七叶有效部位组合物对兔全脑缺血再灌注损伤的保护作用
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摘要
目的观察山楂叶与三七叶有效部位(SS)组合物对兔全脑缺血再灌注损伤的保护作用。方法采用六动脉结扎加股动脉放血法制备家兔全脑缺血再灌注损伤模型,缺血30 min,再灌注2 h。40只家兔随机分为假手术组、模型组、血塞通21. 0 mg/kg组、SS组合物高、低剂量组(6. 370、3. 185 mg/kg)。于再灌2 h后使用南京建成生物工程研究所测定试剂盒检测脑组织中超氧化物歧化酶(SOD)活性、丙二醛(MDA)、谷氨酸含量及血清中谷胱甘肽过氧化物酶(GSH-PX)活力,并进行病理学检查。结果 SS组合物高、低剂量组均能明显增加脑组织SOD活性(P<0. 01; P<0. 05),降低脑组织中MDA含量(P<0. 01)及脑组织中谷氨酸含量(P<0. 01; P<0. 05),同时还可明显增加血清GSH-PX活力(P<0. 05),病理检查结果显示,SS组合物能减少家兔全脑缺血再灌注损伤的脑组织范围,并能保护神经细胞。结论 SS组合物对兔脑缺血再灌注损伤有一定的保护及改善作用,其机制可能与其抗氧化和抑制兴奋性氨基酸释放或拮抗兴奋氨基酸毒性有关。
Objective To observe the protective effects of the effective part (SS) compositions of Hawthorn leaves and Panaxnotoginseng leaves on global cerebral ischemia-reperfusion injury in rabbits. Methods The rabbit model of global cerebral ischemia-reperfusion injury was established by six-artery ligation and femoral artery bleeding. The model was ischemia for 30 min and reperfusionfor 2 h. Forty rabbits were randomly divided into sham-operation,model,Xuesaitong,SS composition high and low dose groups. Theactivity of SOD,content of MDA and glutamic acid in brain tissue and activity of GSH-PX in serum were detected after 2 h reperfusion,and the pathological examination was conducted.Results Both doses of SS composition significantly increased SOD activity in brain tis-sue (P<0.01; P<0.05). The contents of MDA and glutamate in brain tissue (P < 0. 01; P < 0. 05) were decreased,and the activity ofGSH-PX in serum was significantly increased (P<0.05). The results of pathological examination showed that SS composition reducedthe range of brain tissue in rabbits with ischemia-reperfusion injury and protected nerve cells. Conclusions SS composition could pro-tect and ameliorate the cerebral ischemia-reperfusion injury in rabbits,and its mechanism might be related to its antioxidation,inhibitexcitatory amino acid release.
Objective To observe the protective effects of the effective part (SS) compositions of Hawthorn leaves and Panaxnotoginseng leaves on global cerebral ischemia-reperfusion injury in rabbits. Methods The rabbit model of global cerebral ischemia-reperfusion injury was established by six-artery ligation and femoral artery bleeding. The model was ischemia for 30 min and reperfusionfor 2 h. Forty rabbits were randomly divided into sham-operation,model,Xuesaitong,SS composition high and low dose groups. Theactivity of SOD,content of MDA and glutamic acid in brain tissue and activity of GSH-PX in serum were detected after 2 h reperfusion,and the pathological examination was conducted.Results Both doses of SS composition significantly increased SOD activity in brain tis-sue (P<0.01; P<0.05). The contents of MDA and glutamate in brain tissue (P < 0. 01; P < 0. 05) were decreased,and the activity ofGSH-PX in serum was significantly increased (P<0.05). The results of pathological examination showed that SS composition reducedthe range of brain tissue in rabbits with ischemia-reperfusion injury and protected nerve cells. Conclusions SS composition could pro-tect and ameliorate the cerebral ischemia-reperfusion injury in rabbits,and its mechanism might be related to its antioxidation,inhibitexcitatory amino acid release.
引文
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6闫昆明,刘英,张芮,等.新生大鼠缺氧缺血性脑损伤caspase-3表达与自由基损伤机制的研究[J].中国免疫学杂志,2015;31(8):1094-7.
7张佳,黄昌林.中频脉冲电流经皮刺激肝区对运动性疲劳大鼠大脑皮质自由基及尼氏体的影响[J].解放军医学杂志,2015;40(4):331-5.
8宋文婷,刘建勋,姚明江,等.银杏蜜环口服溶液减轻脑缺血再灌注大鼠脑梗死的实验研究[J].世界中医药,2018;13(1):36-40.
9高丽苗,徐响,高蓬勃,等.蜂胶抗脑缺血再灌注损伤作用及机制[J].中国食品学报,2018;18(3):221-5.
10欧册华,张毅,李惠芳.镁预处理对兔全脑缺血神经保护作用的实验研究[J].四川动物,2009;28(3):437-9.
2黄国钧,黄勤挽.医药实验动物模型---制作与应用[M].北京:化学工业出版社生物·医药出版分社,2008:230.
3周代星,邓普珍.家兔急性完全性脑缺血及再灌注后山莨菪碱对脑组织[Ca2+]i和超微结构变化的影响[J].同济医科大学学报,2001;30(6):571-3.
4顾远清,陈显兵,刘爱民,等.动物全脑缺血模型制作的改进[J].湖北民族学院学报·医学版,1999;16(1):63.
5胡文,尹艳艳,王玉婵,等.麝香醒脑滴丸对脑缺血再灌注损伤的保护作用[J].安徽医药,2015;19(1):39-42.
6闫昆明,刘英,张芮,等.新生大鼠缺氧缺血性脑损伤caspase-3表达与自由基损伤机制的研究[J].中国免疫学杂志,2015;31(8):1094-7.
7张佳,黄昌林.中频脉冲电流经皮刺激肝区对运动性疲劳大鼠大脑皮质自由基及尼氏体的影响[J].解放军医学杂志,2015;40(4):331-5.
8宋文婷,刘建勋,姚明江,等.银杏蜜环口服溶液减轻脑缺血再灌注大鼠脑梗死的实验研究[J].世界中医药,2018;13(1):36-40.
9高丽苗,徐响,高蓬勃,等.蜂胶抗脑缺血再灌注损伤作用及机制[J].中国食品学报,2018;18(3):221-5.
10欧册华,张毅,李惠芳.镁预处理对兔全脑缺血神经保护作用的实验研究[J].四川动物,2009;28(3):437-9.