速测卡法快速检测蔬菜中有机磷和氨基甲酸酯类农药残留
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摘要
目的对数字农药残留速测卡速检蔬菜中有机磷和氨基甲酸酯类农药残留方法的可靠性及实用性进行评价。方法采用改良后的速测卡对有机磷及氨基甲酸酯类农药的最低检出限进行试验,以及检测蔬菜样品中农药残留情况,并与标准方法进行对比;并对不同生产批号的产品进行重复性试验。结果速测卡对有机磷和氨基甲酸酯类农药的最低检出限在0.01~3.0 mg/kg;对敌敌畏可达到0.01 mg/kg;对乙酰甲胺磷灵敏度较低,为3.0 mg/kg。检测样品时,速测卡与标准方法阴性符合率为95.2%,阳性符合率为94.1%。5个生产批号的速测卡检测结果一致,批次间重复性较好。结论速测卡法具有快速、准确、方便、灵敏等特点,适用于基层对蔬菜中有机磷及氨基甲酸酯类农药残留进行定性分析。
Objective To evaluate of the reliability and practicability of the method for rapid detection of pesticide residues in vegetables by test paper. Methods The minimum limits of detection for organophosphorus and carbamate pesticides were tested and pesticide residues in vegetable samples were detected by the modified rapid test paper. Then the results were compared with national method. Repeated tests were carried out for different batches of products. Results The limits of detection of the test paper were 0.01-3.0 mg/kg, and the limit of detection of dichlorvos could reach 0.01 mg/kg, while it had low sensitivity of acephate with 3 mg/kg. When the results were compared with national method for detection samples, the negative coincidence rate of rapid test paper and standard method was 95.2%, and the positive rate was 94.1%. The results of 5 batches of rapid test paper were consistent, and the repeatability between batch was better. Conclusion The test paper is rapid, accurate and convenient, which is suitable for rapid detection of pesticides in vegetables.
Objective To evaluate of the reliability and practicability of the method for rapid detection of pesticide residues in vegetables by test paper. Methods The minimum limits of detection for organophosphorus and carbamate pesticides were tested and pesticide residues in vegetable samples were detected by the modified rapid test paper. Then the results were compared with national method. Repeated tests were carried out for different batches of products. Results The limits of detection of the test paper were 0.01-3.0 mg/kg, and the limit of detection of dichlorvos could reach 0.01 mg/kg, while it had low sensitivity of acephate with 3 mg/kg. When the results were compared with national method for detection samples, the negative coincidence rate of rapid test paper and standard method was 95.2%, and the positive rate was 94.1%. The results of 5 batches of rapid test paper were consistent, and the repeatability between batch was better. Conclusion The test paper is rapid, accurate and convenient, which is suitable for rapid detection of pesticides in vegetables.
引文
[1]刘建慧,孙鑫,刘希光,等.果蔬中农药残留现状及检测技术的研究进展[J].食品研究与开发,2014,35(15):119-122.Liu Jh,Sun X,Liu XG,et al.The present situation of pesticide residues in vegetables and fruits and the progress of detection technique[J].Food Res Dev,2014,35(15):119-122.
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[4]朱明松,周晨楠,和劲松,等.基于酶抑制法的农药残留快速比色检测[J].农业工程学报,2014(6):242-248.Zhu MS,Zhou CN,He JS,et al.Rapid colormetric detection of pesticide residues based on enzyme inhibition method[J].Trans Chin Soc Agric Eng,2014(6):242-248.
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[8]朱将伟,邵小龙.气相色谱法测定粮食中有机磷农药残留[J].粮食与饲料工业,2010,(4):61-63.Zhu JW,Shao XL.Determination of organophosphorus pesticide residues in foodstuff by gas chromatography[J].Cereal Feed Ind,2010,(4):61-63.
[9]李志成,郑晓冬,闫新焕,等.快速检测技术在果蔬农药残留检测中的应用[J].中国果蔬,2016,36(12):33-36.Li ZC,Zheng XD,Yan XH,et al.Application of rapid detection technology in pesticide residue detection of fruits and vegetables[J].China Fruit Veget,2016,36(12):33-36.
[10]刘喜德.基于酶抑制法的农药残留快速比色检测研究[J].农技服务,2017,34(6):27.Liu XD.Study on rapid colorimetric detection of pesticide residues based on enzyme inhibition[J].Agric Technol Serv,2017,34(6):27.
[11]翁士状,李淼,李华龙,等.酶抑制分光光度法与表面增强拉曼散射在农药检测中的进展[J].食品安全质量检测学报,2015,6(9):3575-3579.Weng SZ,Li M,Li HL,et al.Progress of enzyme inhibition spectrophotometry and surface enhanced Raman scatter for detection of pesticides[J].J Food Saf Qual,2015,6(9):3575-3579.
[12]GB/T 5009.199-2003蔬菜中有机磷和氨基甲酸酯类农药残留的快速检测[S].GB/T 5009.199-2003 Rapid detection of organophosphorus and carbamate pesticide residues in vegetables[S].
[13]王多加,胡祥娜,周向阳,等.蔬菜农药残留快速检测技术-胆碱酯酶速测卡法[J].食品科学,2003,24(6):109-113.Wang DJ,Hu XN,Zhou XY,et al.Rapid detection technology of pesticide residues in vegetables-Cholinesterase fast test card method[J].Food Sci,2003,24(6):109-113.
[14]汤彤.速测卡法与酶抑制率法快速检测蔬菜中农药残留的对比研究[J].安徽农业科学,2017,45(1):102-104.Tang T.Rapid detection of pesticide residues in vegetables by rapid test card method and enzyme inhibition rate[J].J Anhui Agric Sci,2017,45(1):102-104.
[15]NY/T 761-2008蔬菜和水果中有机磷、有机氯、拟除虫菊酯和氨基甲酸酯类农药多残留的测定[S].NY/T 761-2008 Determination of organophosphorus,organochlorine,pyrethroid and carbamate pesticide residues in vegetables and fruits[S].
[16]颜冬云,蒋新,余贵芬,等.有机磷农药对乙酰胆碱酯酶活性的联合抑制作用[J].农药,2006,45(1):31-34.Yan DY,Jiang X,Yu GF,et al.Combined inhibitory effect of organophosphorus pesticides on acetylcholinesterase activity[J].Agrochemical,2006,45(1):31-34.
[17]王文,刘瑾盛,伟楠,等.采用酶抑制法检测大蒜中农药残留的改进方法[J].食品科学,2013,34(12):135-139.Wang W,Liu JS,Wei N,et al.An improved enzyme inhibition method for detection of peptide residues in garlic[J].Food Sci,2013,34(12):135-139.
[2]蒋雪松,王维琴,许林云,等.农产品/食品中农药残留快速检测方法研究进展[J].农业工程学报,2016,32(20):267-274.Jiang XS,Wang WQ,Xu LY,et al.Review on rapid detection of pesticide residues in agricultural and food products[J].Trans Chin Soc Agric Eng,2016,32(20):267-274.
[3]万辉.农产品农药残留检测技术及应用之研究[J].农村经济与科技,2017,28(14):39.Wan H.Study on detection technology and application of pesticide residues in agricultural products[J].Rural Econ Sci Technol,2017,28(14):39.
[4]朱明松,周晨楠,和劲松,等.基于酶抑制法的农药残留快速比色检测[J].农业工程学报,2014(6):242-248.Zhu MS,Zhou CN,He JS,et al.Rapid colormetric detection of pesticide residues based on enzyme inhibition method[J].Trans Chin Soc Agric Eng,2014(6):242-248.
[5]Ecobichon DJ.Pesticide use in developing countries[J].Toxicology,2001,160(1):27-33.
[6]王淑梅,王江,齐海鸥.2006年陕西省六地市蔬菜水果农药残留状况[J].职业与健康,2008,24(16):1619-1621.Wang SM,Wang J,Qi HO.Situation of pesticide residues in fruits and vegetables in six cities of Shanxi province in 2006[J].Occup Health,2008,24(16):1619-1621.
[7]Lee SA,Dai Q,Zheng W,et al.Association of serum concentration of organochlorine pesticides with dietary intake and other lifestyle factors among urban Chinese women[J].Environ Int,2007,33(2):157-163.
[8]朱将伟,邵小龙.气相色谱法测定粮食中有机磷农药残留[J].粮食与饲料工业,2010,(4):61-63.Zhu JW,Shao XL.Determination of organophosphorus pesticide residues in foodstuff by gas chromatography[J].Cereal Feed Ind,2010,(4):61-63.
[9]李志成,郑晓冬,闫新焕,等.快速检测技术在果蔬农药残留检测中的应用[J].中国果蔬,2016,36(12):33-36.Li ZC,Zheng XD,Yan XH,et al.Application of rapid detection technology in pesticide residue detection of fruits and vegetables[J].China Fruit Veget,2016,36(12):33-36.
[10]刘喜德.基于酶抑制法的农药残留快速比色检测研究[J].农技服务,2017,34(6):27.Liu XD.Study on rapid colorimetric detection of pesticide residues based on enzyme inhibition[J].Agric Technol Serv,2017,34(6):27.
[11]翁士状,李淼,李华龙,等.酶抑制分光光度法与表面增强拉曼散射在农药检测中的进展[J].食品安全质量检测学报,2015,6(9):3575-3579.Weng SZ,Li M,Li HL,et al.Progress of enzyme inhibition spectrophotometry and surface enhanced Raman scatter for detection of pesticides[J].J Food Saf Qual,2015,6(9):3575-3579.
[12]GB/T 5009.199-2003蔬菜中有机磷和氨基甲酸酯类农药残留的快速检测[S].GB/T 5009.199-2003 Rapid detection of organophosphorus and carbamate pesticide residues in vegetables[S].
[13]王多加,胡祥娜,周向阳,等.蔬菜农药残留快速检测技术-胆碱酯酶速测卡法[J].食品科学,2003,24(6):109-113.Wang DJ,Hu XN,Zhou XY,et al.Rapid detection technology of pesticide residues in vegetables-Cholinesterase fast test card method[J].Food Sci,2003,24(6):109-113.
[14]汤彤.速测卡法与酶抑制率法快速检测蔬菜中农药残留的对比研究[J].安徽农业科学,2017,45(1):102-104.Tang T.Rapid detection of pesticide residues in vegetables by rapid test card method and enzyme inhibition rate[J].J Anhui Agric Sci,2017,45(1):102-104.
[15]NY/T 761-2008蔬菜和水果中有机磷、有机氯、拟除虫菊酯和氨基甲酸酯类农药多残留的测定[S].NY/T 761-2008 Determination of organophosphorus,organochlorine,pyrethroid and carbamate pesticide residues in vegetables and fruits[S].
[16]颜冬云,蒋新,余贵芬,等.有机磷农药对乙酰胆碱酯酶活性的联合抑制作用[J].农药,2006,45(1):31-34.Yan DY,Jiang X,Yu GF,et al.Combined inhibitory effect of organophosphorus pesticides on acetylcholinesterase activity[J].Agrochemical,2006,45(1):31-34.
[17]王文,刘瑾盛,伟楠,等.采用酶抑制法检测大蒜中农药残留的改进方法[J].食品科学,2013,34(12):135-139.Wang W,Liu JS,Wei N,et al.An improved enzyme inhibition method for detection of peptide residues in garlic[J].Food Sci,2013,34(12):135-139.