红芪黄酮气溶胶给药与灌胃给药对COPD大鼠的影响
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摘要
目的:比较红芪黄酮灌胃给药及气溶胶给药两种不同的给药方法对大鼠慢性阻塞性肺病模型(COPD)的治疗作用。方法:选取40只SPF级雄性SD大鼠,随机分为空白对照组、模型对照组、红芪黄酮灌胃给药组及红芪黄酮气溶胶给药组,每组10只。空白对照组正常饮食喂养,不加任何处理措施。其余3组每天于小动物烟雾气溶胶染毒暴露系统吸入新鲜的香烟烟雾染毒造模,每天染毒30分钟,造模28天。造模后第7天开始给药,共21天。红芪黄酮灌胃给药组每日灌胃1 m L/100 g,37.41 mg/kg,给药,红芪黄酮气溶胶给药组气溶胶给药,气溶胶浓度保持在3.5～4.0 mg/m~3,给药时间约40分钟。实验结束后,检测清醒及麻醉状态下各组动物肺功能,观察肺组织切片病理形态。结果:清醒状态及麻醉状态下造模组动物肺功能指标与空白对照组比较,均有显著性变化,差异有统计学意义(P<0.05);与模型对照组比较,各给药组显著改善肺功能相关指标,且气溶胶给药组作用更为显著。病理切片结果显示,与空白对照组比较,造模组出现不同程度的异常改变;与模型对照组比较,各给药组病理形态有显著改善,且气溶胶给药组作用更为显著。结论:气溶胶给药与灌胃给药相比,对COPD模型大鼠的治疗作用更为显著,气溶胶给药技术对于COPD的治疗具有重要意义。
Objective: To compare therapeutic effects of astragalus flavone in the treatment for COPD rats by aerosal and intragastric administration. Methods: Forty rats were chosen and randomized into normal control group,model control group, intragastric administration group and aerosal administration group, ten rats each group. Blank control group accepted normal diet and left unhandled. The rats in the other three groups were prepared into the models by inhaling fresh cigarette smoke in animal smoke exposure system, exposing for 30 minutes each day, for28 days consecutively. The rat model were medicated since the seventh day after modelling, for 21 days. Intragastric administration group were drenched each day, 1 m L/100 g, 37.41 mg/kg, aerosal administration group were medicated by aerosal, the concentrations kept at 3.5～4.0 mg/m~3, medicated for around 40 minutes. By the end of the experiment, to detect pulmonary function of the rats in different groups when the rats were awake and under the state of anesthesia, to observe pathological morphology of pulmonary tissue. Results: Compared with blank control group,the indexes of pulmonary function in the rats under the waking and anesthesia states of the modelling group showed significant changes, the difference showed statistical meaning(P <0.05); compared with model control group,different medication groups significantly improve the related indexes of pulmonary function, and the group of aerosal administration indicated more notable effects. The results of pathological slices presented, compared with blank control group, the modelling group displayed abnormal changes to different extents; compared with model control group, pathological morphology of different medication groups improved apparently, and the effects of aerosal administration group the most notable. Conclusion: Compared with intragastric administration, aerosal administration shows more notable effects on COPD rat model, the technology of aerosal administration is significant for the therapy of COPD.
Objective: To compare therapeutic effects of astragalus flavone in the treatment for COPD rats by aerosal and intragastric administration. Methods: Forty rats were chosen and randomized into normal control group,model control group, intragastric administration group and aerosal administration group, ten rats each group. Blank control group accepted normal diet and left unhandled. The rats in the other three groups were prepared into the models by inhaling fresh cigarette smoke in animal smoke exposure system, exposing for 30 minutes each day, for28 days consecutively. The rat model were medicated since the seventh day after modelling, for 21 days. Intragastric administration group were drenched each day, 1 m L/100 g, 37.41 mg/kg, aerosal administration group were medicated by aerosal, the concentrations kept at 3.5～4.0 mg/m~3, medicated for around 40 minutes. By the end of the experiment, to detect pulmonary function of the rats in different groups when the rats were awake and under the state of anesthesia, to observe pathological morphology of pulmonary tissue. Results: Compared with blank control group,the indexes of pulmonary function in the rats under the waking and anesthesia states of the modelling group showed significant changes, the difference showed statistical meaning(P <0.05); compared with model control group,different medication groups significantly improve the related indexes of pulmonary function, and the group of aerosal administration indicated more notable effects. The results of pathological slices presented, compared with blank control group, the modelling group displayed abnormal changes to different extents; compared with model control group, pathological morphology of different medication groups improved apparently, and the effects of aerosal administration group the most notable. Conclusion: Compared with intragastric administration, aerosal administration shows more notable effects on COPD rat model, the technology of aerosal administration is significant for the therapy of COPD.
引文
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[2]张珍祥,张惠兰.慢性阻塞性肺疾病急性加重期的定义及治疗[J].实用医学进修杂志,2009,37(3):129-130.
[3]蔺兴遥,张毅,李娟,等.黄芪黄酮与红芪黄酮对肺间质纤维化模型大鼠肺功能影响的对比研究[J].中成药,2013,35(8):1770-1773.
[4]李娟,蔺兴遥,李金田,等.红芪黄酮对肺间质纤维化模型大鼠肺功能的影响[J].中药材,2013,36(5):771-775.
[5]苏韫,张毅,李娟,等.红芪黄酮对肺纤维化模型大鼠基质金属蛋白酶-2及其抑制剂-1蛋白表达的影响[J].中国中医药信息杂志,2015,22(1):47-49.
[6]舍雅莉,苏韫,张毅,等.红芪黄酮对肺间质纤维化模型大鼠微血管密度的影响[J].中国中医药信息杂志,2014,21(4):43-45.
[7]程羽,陶伟利,张晓梅,等.建立慢性阻塞性肺疾病动物模型的实验研究[J].世界中西医结合杂志,2016,11(9):445-448.