槐叶决明SSR标记开发与应用
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摘要
决明属植物全世界约590种,我国约10种,入药用的约19种。很多种间形态差异微小,急需开发简单高效的方法进行鉴定和区分。本研究从槐叶决明转录组数据库中,共发掘15 753个SSR位点,成功设计10110个SSR标记;随机对其中的50对SSR标记进行验证,其中有25对在槐叶决明、望江南和小决明3个种的9个地理来源的种质资源中具有清晰的电泳条带,平均等位基因2.72个,平均遗传丰富度和PIC分别为0.45和0.39。各试验材料间基于SSR分子标记的遗传距离在0.111~0.963,其中槐叶决明、小决明和望江南种间,以及小决明和望江南种内不同地理来源之间均存在较大遗传差异。本研究为决明属种质资源的遗传多样性分析以及种质资源鉴定奠定了基础。
引文
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[2]连文琰.中国决明属药用植物简报[J].中草药,1986,17(70):27.
[3]韩文雯,李天祥.望江南子的生药学研究[J].天津药学,2011,23(3):49-51.
[4]SINGH R,HUSSAIN S,VERMA R,et al.Anti-mycobacterial screening of five Indian medicinal plants and partial purification of active extracts of Cassia sophera and Urtica dioica[J].Asian Pacific Journal of Tropical Medicine,2013,6(5):366-371.
[5]TIWARI R D,MISRA G.Chemical examination of the flowers of Cassia sophera[J].Planta Medica,1975,28(2):182-185.
[6]ZHAO Y,LIU J P,LU D,et al.A novel cycloartane triterpene glycoside from the seeds of Cassia sophera L.[J].Natural Product Research,2007,21(6):494-499.
[7]宋贤丽,郭宝林,刘克武,等.6种决明属药用植物种子毛细管电泳法鉴别[J].中国中药杂志,2003,28(6):14-19.
[8]李婷,冯占民,杨巡纭,等.决明属植物的化学成分及药理作用研究进展[J].林产化学与工业,2012,32(6):107-118.
[9]胡勇,陈丽琼,朱霞,等.决明属化学成分与药理作用研究进展[J].现代中药研究与实践,2013,27(5):69-71.
[10]王静,张跃进,梁宗锁,等.决明DNA提取、SRAP-PCR优化及引物筛选[J].西北农业学报,2014,23(2):137-142.
[11]KALIA R K,RAI M K,KALIA S,et al.Microsatellite markers:an overview of the recent progress in plants[J].Euphytica,2011,177(3):309-334.
[12]JAIN A,CHAUDHARY S,SHARMA P C.Mining of microsatellites using next generation sequencing of seabuckthorn(Hippophae rhamnoides L.)transcriptome[J].Physiology and Molecular Biology of Plants:An International Journal of Functional Plant Biology,2014,20(1):115-123.
[13]LI D M,ZHAO C Y,LIU X R,et al.De novo assembly and characterization of the root transcriptome and development of simple sequence repeat markers in Paphiopedilum concolor[J].Genetics and Molecular Research,2015,14(2):6189-6201.
[14]ZENG S,XIAO G,GUO J,et al.Development of a EST dataset and characterization of EST-SSRs in a traditional Chinese medicinal plant,Epimedium sagittatum(Sieb.Et Zucc.)Maxim[J].BMC Genomics,2010,11:94.
[15]DOYLE J J,DOYLE J L.A rapid DNA isolation procedure for small quantities of fresh leaf tissue[J].Phytochemical Bulletin,1987,19(1):11-15.
[16]LIU K,MUSE S V.Power Marker:an integrated analysis environment for genetic marker analysis[J].Bioinformatics,2005,21(9):2128-2129.