ADAM33基因F+1位点基因多态性与小儿哮喘的关系
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摘要
目的分析ADAM33基因F+1位点基因多态性与小儿哮喘的关系,为临床诊断小儿哮喘提供依据。方法选取2014年6月-2017年6月哈尔滨医科大学附属第二医院诊治的100例小儿哮喘患儿为观察组,同期该院诊断的健康体检小儿100例为对照组。分析两组研究对象ADAM33基因F+1位点是否满足Hardy-Weinberg遗传平衡定律,检测两组研究对象的C和T等位基因的频率,并分析ADAM33基因F+1位点多态性与哮喘发病的关系。结果所有研究对象的ADAM33基因F+1位点均满足Hardy-Weinberg遗传平衡定律。两组C和T等位基因频率比较,差异无统计学意义(P>0.05);且F+1位点多态性对哮喘相对危险无明显影响(P>0.05)。结论 ADAM33基因F+1位点基因多态性与小儿哮喘发病无显著关系。
Objective To analyze the relationship between gene polymorphism of ADAM33 F+1 locus and asthma in children,provide a basis for clinical diagnosis of asthma in children. Methods One hundred children with asthma diagnosed and treated in the Second Hospital of Harbin Medical University from June 2014 to June 2017 were selected as observation group,and one hundred children receiving physical examination in the hospital during the same period were selected as control group. The coincidences of ADAM33 F+1 locus and HardyWeinberg equilibrium in the two groups were analyzed. The frequencies of C and T alleles in the two groups were detected. The relationship between gene polymorphism of ADAM33 F+1 locus and onset of asthma in children was analyzed. Results ADAM33 F+1 locus met HardyWeinberg equilibrium among all the children. There was no statistically significant difference in the frequencies of C and T alleles between the two groups( P > 0. 05). Gene polymorphism of ADAM33 F + 1 locus had no significant impact on asthma( P > 0. 05).Conclusion Gene polymorphism of ADAM33 F + 1 locus is not significantly correlated with asthma in children.
Objective To analyze the relationship between gene polymorphism of ADAM33 F+1 locus and asthma in children,provide a basis for clinical diagnosis of asthma in children. Methods One hundred children with asthma diagnosed and treated in the Second Hospital of Harbin Medical University from June 2014 to June 2017 were selected as observation group,and one hundred children receiving physical examination in the hospital during the same period were selected as control group. The coincidences of ADAM33 F+1 locus and HardyWeinberg equilibrium in the two groups were analyzed. The frequencies of C and T alleles in the two groups were detected. The relationship between gene polymorphism of ADAM33 F+1 locus and onset of asthma in children was analyzed. Results ADAM33 F+1 locus met HardyWeinberg equilibrium among all the children. There was no statistically significant difference in the frequencies of C and T alleles between the two groups( P > 0. 05). Gene polymorphism of ADAM33 F + 1 locus had no significant impact on asthma( P > 0. 05).Conclusion Gene polymorphism of ADAM33 F + 1 locus is not significantly correlated with asthma in children.
引文
[1]蒋宝安,屈斌,郑辉,等.支气管哮喘的发病机制及药物治疗研究进展[J].中国当代医药,2015,22(13):35-37.
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[7]王建荣,吴英杰,多力坤·木扎帕尔.乌鲁木齐地区维、汉族儿童哮喘与ADAM33基因多态性的相关性[J].临床儿科杂志,2016,34(12):926-929.
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[10]米合热古力·司马义,胡昕,王晶,等.解整合素-金属蛋白酶33基因F1、L1、T1位点多态性与维吾尔族成人哮喘的相关性研究[J].新疆医科大学学报,2016,39(3):257-260.
[11]方丽萍,陈敏,张方,等.解整合素-金属蛋白酶33在不同严重程度哮喘患者血清中的表达水平及其与气道慢性炎症的关系[J].医学研究生学报,2017,30(5):521-524.
[12]王爱华,陈文君,徐建亚,等.固本防哮饮含药血清对IL-4刺激的人胚肺成纤维细胞中ADAM33、ETS1表达的影响[J].中华中医药杂志,2017,32(4):1742-1746.
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[14]吕既寿,江丽汗·阿黑哈提,王建荣,等.ADAM33基因V4位点多态性和维生素D水平与乌鲁木齐地区支气管哮喘儿童相关性研究[J].中国儿童保健杂志,2017,25(3):248-250.
[15]周兆山,王燕青,朱习军,等.ADAM33基因rs2280090、rs487377、rs2787094多态性与大鱼际掌纹特应征的相关性研究[J].中华中医药学刊,2017,35(2):265-268.
[2]雷雪春,朱黎明,戴爱国.药物基因组学与哮喘个体化给药研究进展[J].中国临床药理学杂志,2014,30(9):871-874.
[3]陈凯鹏,芦爱萍.儿童及成人哮喘基因研究新进展[J].医学综述,2012,18(1):38-40.
[4]李宏彬,赵兵,席景砖.ADAM33基因S2位点多态性与支气管哮喘易感性关系的Meta分析[J].现代预防医学,2014,41(5):773-776.
[5]于水莲,曲书强.ADAM33基因与哮喘及其严重程度的关系[J].中国优生与遗传杂志,2014,22(3):6-8.
[6]王建荣,吕既寿,多力坤·木扎帕尔.ADAM33基因F+1多态性、Ig E、维生素D与乌鲁木齐地区维吾尔族、汉族哮喘儿童的相关性研究[J].新疆医科大学学报,2017,40(1):62-65.
[7]王建荣,吴英杰,多力坤·木扎帕尔.乌鲁木齐地区维、汉族儿童哮喘与ADAM33基因多态性的相关性[J].临床儿科杂志,2016,34(12):926-929.
[8]王建荣,余蓉,吴英杰.乌鲁木齐地区维吾尔族、汉族哮喘儿童ADAM33 V4基因多态性与Ig E水平的相关性研究[J].新疆医学,2017,47(2):131-134.
[9]张伟,宋秀婷,徐益恒,等.ADAM33基因T1、S2位点多态性在中国人支气管哮喘易感性的Meta分析[J].昆明医科大学学报,2016,37(6):25-30.
[10]米合热古力·司马义,胡昕,王晶,等.解整合素-金属蛋白酶33基因F1、L1、T1位点多态性与维吾尔族成人哮喘的相关性研究[J].新疆医科大学学报,2016,39(3):257-260.
[11]方丽萍,陈敏,张方,等.解整合素-金属蛋白酶33在不同严重程度哮喘患者血清中的表达水平及其与气道慢性炎症的关系[J].医学研究生学报,2017,30(5):521-524.
[12]王爱华,陈文君,徐建亚,等.固本防哮饮含药血清对IL-4刺激的人胚肺成纤维细胞中ADAM33、ETS1表达的影响[J].中华中医药杂志,2017,32(4):1742-1746.
[13]王建荣,吕既寿,陈春花.乌鲁木齐地区维汉哮喘儿童ADAM33基因多态性与Ig E、维生素D的相关性研究[J].标记免疫分析与临床,2016,23(10):1153-1157+1232.
[14]吕既寿,江丽汗·阿黑哈提,王建荣,等.ADAM33基因V4位点多态性和维生素D水平与乌鲁木齐地区支气管哮喘儿童相关性研究[J].中国儿童保健杂志,2017,25(3):248-250.
[15]周兆山,王燕青,朱习军,等.ADAM33基因rs2280090、rs487377、rs2787094多态性与大鱼际掌纹特应征的相关性研究[J].中华中医药学刊,2017,35(2):265-268.