红外光谱和逐步判别分析应用于金银花品种和产地的鉴别
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摘要
基于傅里叶变换红外光谱技术,利用逐步判别分析法对金银花品种和产地进行鉴别研究。采集、测试了5个产地9个品种150份金银花样本的红外光谱,并计算了各样品红外光谱的一阶导数光谱和二阶导数光谱。分别选用不同的样本组成训练集和检验集,以1800～900 cm~(-1)、1500～700 cm~(-1)和1200～700 cm~(-1)波数范围的红外光谱、一阶导数光谱和二阶导数光谱数据为判别变量建立判别模型对金银花的品种和产地进行鉴别。判别结果显示,以1800～900 cm~(-1)波数范围的二阶导数光谱数据为判别变量建立的模型鉴别效果相对较好,对品种和产地的鉴别正确率依次达93.20%和96.13%。研究结果表明,采用逐步判别模式识别可以很好地鉴别不同品种和产地的金银花,方法可行有效,可为金银花品种和产地朔源提供方法。
The Fourier Transform Infrared Spectroscopy of 150 Honeysuckle specimens of nine varieties collected from five regions were obtained to discriminate the sample variety and geographical origin.All specimens were randomly divided into a training set and a validation set.The discrimination analysis based on the original spectra,first-derivate spectra and second-derivate spectra revealed that the model based on the second-derivate spectra has the highest accuracy,and the model based on the waveband of 1800～900 cm~(-1) was better than that of 1500～700 cm~(-1) and 1200～700 cm~(-1),which yielded correct rate of 93.20% for distinguishing variety and 96.13% for identifying origin.The study demonstrated that Fourier Transform Infrared Spectroscopy combining with discrimination analysis might be used as an effective way to distinguish Honeysuckle variety and origins.
The Fourier Transform Infrared Spectroscopy of 150 Honeysuckle specimens of nine varieties collected from five regions were obtained to discriminate the sample variety and geographical origin.All specimens were randomly divided into a training set and a validation set.The discrimination analysis based on the original spectra,first-derivate spectra and second-derivate spectra revealed that the model based on the second-derivate spectra has the highest accuracy,and the model based on the waveband of 1800～900 cm~(-1) was better than that of 1500～700 cm~(-1) and 1200～700 cm~(-1),which yielded correct rate of 93.20% for distinguishing variety and 96.13% for identifying origin.The study demonstrated that Fourier Transform Infrared Spectroscopy combining with discrimination analysis might be used as an effective way to distinguish Honeysuckle variety and origins.
引文
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[17] 张燕,解凤岭,郭兰萍,等.不同冬剪方式对金银花生长、产量和质量影响的研究[J].中国中药杂志,2012,37(22):3371-3374.(ZHANG Yan,XIE Fengling,GUO Lanping,et al.Different pruning to yield and quanlity of Lonicerea japonicae flos[J].China Journal of Chinese Materia Medica,2012,37(22):3371-3374)
[2] 钟晓凤.金银花药用成分及现代临床药理分析[J].光明中医,2016,31(06):887-888.(ZHONG Xiaofeng.Analysis of medicinal component and modern clinical pharmacology of Lonicerae japonica flos[J].Guangming Journal of Chinese Medicine,2016,31(06):887-888)
[3] 蹇黎.金银花资源的SWOT分析[J].现代园艺,2016,(19):35-36.(JIAN Li.SWOT analysis of resources of Lonicera japonica[J].Modern Horticulture,2016,(19):35-36)
[4] 王亚丹,杨建波,戴忠,等.中药金银花的研究进展[J].药物分析杂志,2014,34(11):1928-1935.(WANG Yadan,YANG Jianbo,DAI Zhong,et al.Research progress on Lonicerae japonica flos[J].Chinese Journal of Pharmaceutical Analysis,2014,34(11):1928-1935)
[5] 蒋超,袁媛,刘贵明,等.基于EST-SSR的金银花分子鉴别方法研究[J].药学学报,2012,47(6):803-810.(JIANG Chao,YUAN Yuan,LIU Guiming,et al.EST-SSR identification of Lonicera japonica Thunb[J].Acta Pharmaceutica Sinica,2012,47(6):803-810)
[6] 辛立红,吕玲霞,管仁炜,等.金银花质量的影响因素及控制措施[J].山东农业科学,2017,49(3):154-157.(XIN Lihong,LV Lingxia,GUAN Renwei,et al.Influencing factors and control measures of honeysuckle quality[J].Shandong Agricultural Sciences,2017,49(3):154-157)
[7] 孙麒,王国华.高效液相色谱指纹图谱在中药金银花质量控制中的价值研究[J].四川中医,2015,33(02):95-97.(SUN Qi,WANG Guohua.Study on quality control of honeysuckle by HPLS fingerprinting[J].Journal of Sichuan of Traditional Chinese Medicine,2015,33(02):95-97)
[8] 耿姝,陈勇,金叶,等.基于近红外技术的金银花药材多指标成分快速检测[J].中国现代应用药学,2016,33(2):144-150.(GENG Shu,CHEN Yong,JIN Ye,et al.Rapid quantitative determination of multi-Indicators in Lonicerae japonicae flos by near-infrared spectroscopy[J].Chinese Journal of Modern Applied Pharmacy,2016,33(2):144-150)
[9] 李建军,王君,任美玲,等.金银花新品系与主栽品种产量及其指标成分分析[J].河南农业大学学报,2016,50(01):19-24.(LI Jianjun,WANG Jun,REN Meiling,et al.Analysis of yield and index component of Lonicera japonica new strain and major cultivars[J].Journal of Henan Agricultural University,2016,50(01):19-24)
[10] 崔志伟,王康才,郑辉,等.DNA条形码序列对不同品种金银花的鉴定[J].江苏农业科学,2013,41(8):43-45.(CUI Zhiwei,WANG Kangcai,ZHENG Hui,et al.Identification of the variety of Lonicera japonica based on DNA barcoding[J].Jiangsu Agricultural Sciences,2013,41(8):43-45)
[11] 朱英杰,陈士林,姚辉,等.重楼属药用植物DNA条形码鉴定研究[J].药学学报,2010,45(3):376-382.(ZHU Ying-ie,CHEN Shilin,YAO Hui,et al.DNA barcoding the medicinal plants of the genus paris[J].ACTA Pharmaceutica Sinica,2010,45(3):376-382)
[12] 龚海燕,罗晓,雷敬卫,等.不同产地金银花中红外光谱分析[J].中医学报,2016,31(01),96-98.(GONG Haiyan,LUO Xiao,LEI Jingwei,et al.Infrared spectroscopy analysis of Jinyinhua(flos lonicerae) of different origin[J].ACTA Chinese Medicine,2016,31(01),96-98)
[13] 宇传华.SPSS与统计分析(第2版)[M].北京:电子工业出版社,2014.(YU Chuanhua.SPSS application and statistical analysis sassafras.Beijing:Publishing House of Electronics Industry,2014.)
[14] 中华人民共和国药典委员会.中华人民共和国药典[M].北京:中国医药科技出版社,2015.(Chinese Pharmacopoeia Commission.Pharmacopoeia of the People's Republic of China[M].Beingjing:China Medical Science Press,2015)
[15] 赵东岳,李勇,丁万隆.我国金银花栽培品种的遗传多样性[J].世界科学技术-中医药现代化,2011,13(4):650-653.(ZHAO Dongyue,LI Yong,DING Wanlong.Genetic diversity of Lonicera japonica cultivars in China[J].World Science and Technology/Modernization of Traditional Chinese Medicine and Materia Medica,2011,13(4):650-653)
[16] 周照鑫.檫树选作行道树的可行性分析研究[J].绿色科技,2013,(7):110-112.(ZHOU Zhaoxin.The feasibilitystudy of sassafras as shade tree[J].Journal of Green Science and Technology,2013,(7):110-112)
[17] 张燕,解凤岭,郭兰萍,等.不同冬剪方式对金银花生长、产量和质量影响的研究[J].中国中药杂志,2012,37(22):3371-3374.(ZHANG Yan,XIE Fengling,GUO Lanping,et al.Different pruning to yield and quanlity of Lonicerea japonicae flos[J].China Journal of Chinese Materia Medica,2012,37(22):3371-3374)