小麦麸皮水不溶性阿拉伯木聚糖提取及其酶解产物分析
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摘要
以小麦麸皮为原料,采用碱提法对小麦麸皮中的水不溶性阿拉伯木聚糖进行提取。以水不溶性阿拉伯木聚糖得率为响应值,采用单因素试验和响应面分析法对其提取工艺进行优化,并利用不同木聚糖酶对其进行酶解,采用薄层色谱(TLC)法对酶解产物进行分析。结果表明,水不溶性阿拉伯木聚糖的最佳提取工艺为料液比1∶193(g∶mL)、提取温度61℃、提取时间5 h。在此最优提取工艺条件下,水不溶性阿拉伯木聚糖的得率为51.61%,较优化前提高20.71%。用不同种类木聚糖酶对提取的水不溶性阿拉伯木聚糖进行酶解,TLC分析结果表明,链霉菌10904来源的木聚糖酶A对水不溶性阿拉伯木聚糖有较好的底物特异性,酶解产物丰富且以木二糖为主,为阿拉伯低聚木糖的制备提供理论依据。
Using wheat bran as raw material, the water insoluble arabinoxylan in wheat bran was extracted by alkaline extraction method. Using the yield of water insoluble arabinoxylan as response value, the extraction process was optimized by single factor tests and response surface analysis method. The water insoluble arabinoxylan was hydrolyzed by different xylanases, and the enzymatic hydrolyzed products were analyzed by thin layer chromatography method. The results showed that the optimal extraction process of water insoluble arabinoxylan was determined as follows: material to liquid ratio 1∶193(g∶ml), extraction temperature 61 ℃, time 5 h. Under the optimal extraction process conditions, the yield of water insoluble arabinoxylan was 51.61%, which increased by 20.71% than that of before optimization. The extracted water insoluble arabinoxylan was hydrolyzed by different kinds of xylanases, the results of thin layer chromatography analysis showed that the xylanase A from Streptomycete 10904 had good substrate specificity for the extracted water insoluble arabinoxylan, the enzymatic hydrolysis products were rich and were mainly xylobiose, which provided a theoretical basis for the preparation of arabinoxylan oligosaccharides.
Using wheat bran as raw material, the water insoluble arabinoxylan in wheat bran was extracted by alkaline extraction method. Using the yield of water insoluble arabinoxylan as response value, the extraction process was optimized by single factor tests and response surface analysis method. The water insoluble arabinoxylan was hydrolyzed by different xylanases, and the enzymatic hydrolyzed products were analyzed by thin layer chromatography method. The results showed that the optimal extraction process of water insoluble arabinoxylan was determined as follows: material to liquid ratio 1∶193(g∶ml), extraction temperature 61 ℃, time 5 h. Under the optimal extraction process conditions, the yield of water insoluble arabinoxylan was 51.61%, which increased by 20.71% than that of before optimization. The extracted water insoluble arabinoxylan was hydrolyzed by different kinds of xylanases, the results of thin layer chromatography analysis showed that the xylanase A from Streptomycete 10904 had good substrate specificity for the extracted water insoluble arabinoxylan, the enzymatic hydrolysis products were rich and were mainly xylobiose, which provided a theoretical basis for the preparation of arabinoxylan oligosaccharides.
引文
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[2]刘涛.小麦麸皮深加工的探索[J].现代面粉工业,2018,32(1):42-43.
[3]孙军勇,陆健.大麦麦芽阿拉伯木聚糖的研究进展[J].食品与发酵工业,2017,43(5):261-267.
[4]SUN Y,CUI S W,GU X,et al.Isolation and structural characterization of water unextractable arabinoxylans from Chinese black-grained wheat bran[J].Carbohyd Polym,2011,85(3):615-621.
[5]WANG J,SUN B,LIU Y,et al.Optimization of ultrasound-assisted enzymatic extraction of arabinoxylan from wheat bran[J].Food Chem,2008,106(2):804-810.
[6]DU C,CAMPBELL G M,MISAILIDIS N,et al.Evaluating the feasibility of commercial arabinoxylan production in the context of a wheat biorefinery principally producing ethanol.Part 1.Experimental studies of arabinoxylan extraction from wheat bran[J].Chem Eng Res Des,2009,87(9):1232-1238.
[7]KALE M S,HAMAKER B R,CAMPANELLA O H.Alkaline extraction conditions determine gelling properties of corn bran arabinoxylans[J].Food Hydrocolloid,2013,31(1):121-126.
[8]ESCARNOT E,AGUEDO M,AGNEESSENS R,et al.Extraction and characterization of water-extractable and water-unextractable arabinoxylans from spelt bran:Study of the hydrolysis conditions for monosaccharides analysis[J].J Cereal Sci,2011,53(1):45-52.
[9]杨莎,郭晓娜,朱科学,等.碱提方法对小麦麸皮阿拉伯木聚糖结构及面团特性的影响[J].中国粮油学报,2017(11):8-13.
[10]郑学玲.小麦麸皮戊聚糖的分离制备,理化性质及功能特性研究[D].无锡:江南大学,2002.
[11]马福敏,徐美玲,郭小瑞,等.全麦粉水不溶性戊聚糖碱法提取工艺的研究[J].食品工业科技,2013,34(3):1-9.
[12]雷钊,尹达菲,袁建敏.阿拉伯木聚糖和阿拉伯低聚木糖的益生功能研究进展[J].动物营养学报,2017,29(2):365-373.
[13]马福敏.阿拉伯木聚糖的功能性质及其应用研究[D].无锡:江南大学,2009.
[14]赵梦丽.小麦阿拉伯木聚糖益生与免疫调节活性研究[D].北京:中国农业科学院,2015.
[15]国家食品药品监督管理总局.GB/T 5009.5-2016食品中蛋白质的测定[S].北京:中国标准出版社,2016.
[16]国家卫生和计划生育委员会.GB/T 5009.3-2016食品中水分的测定[S].北京:中国标准出版社,2016.
[17]张晓娜,宋琳琳,冉军舰.正交试验优化玉米皮中阿拉伯木聚糖提取条件[J].科技信息,2009(26):14-15.
[18]迟燕平,胡楠楠,卢敏,等.米糠阿拉伯木聚糖提取工艺优化[J].东北农业科学,2016,41(5):97-101.
[19]张梅红.小麦麸皮阿拉伯木聚糖的制备及益生活性研究[D].北京:中国农业科学院,2013.
[20]熊科,熊苏玥,崔晓亭,等.链霉菌L10608产木聚糖酶纯化及特异水解底物生成益生元型产物研究[J].食品与发酵工业,2017(5):54-60.
[21]QI Y,YUE G,HUANG Y P,et al.Identification of three important amino acid residues of xylanase Afxyn A from Aspergillus fumigatus for enzyme activity and formation of xylobiose as the major product[J].Process Biochem,2015,50(4):571-581.