金花茶叶多酚类成分HPLC指纹图谱研究
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摘要
为了建立金花茶叶的HPLC指纹图谱,为评价其质量提供依据,本研究采用HPLC法测定,使用Agilent ZORBAX C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-0.1%磷酸水溶液为流动相,梯度洗脱,流速1 m L/min,柱温30℃,检测波长254 nm,采用"中药色谱指纹图谱相似度评价系统(2012.1版)"及SPSS软件对10个批次样品进行数据处理,并用UPLC-QTOF-MS对部分共有峰进行初步归属。研究结果表明:10批金花茶叶共标定共有峰10个,通过UPLC-QTOF-MS进一步分析,鉴定出其中5个多酚类成分分别为3'-甲基-鞣花酸-4'-葡萄糖苷、鞣花酸、Okicamellia、3'-甲基鞣花酸、3,4-o,o-次甲基-鞣花酸。10批样品指纹图谱相似度均>0.90。通过聚类分析及主成分分析,将10批样品分为4类。本研究为金花茶药材的全面质量评价提供参考。
In order to establish the HPLC fingerprint of the leaves of Camellia sinensis Chi to provide a basis for evaluating its quality,this study was performed by HPLC using an Agilent ZORBAX C18 column( 4.6 mm × 250 mm,5μm) with gradient mobile phase of acetonitrile-0.1% phosphoric acid at a flow rate of 1 m L/min. The detection wavelength was set at 254 nm,and the column temperature was 30℃. Ten batches of Camellia nitidissima Chi samples were processed by similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine( 2012.1 Version) and SPSS software,and some common peaks were summarized preliminarily based on the UPLCQTOF-MS spectra. 10 common peaks were collected from 10 batches of Camellia nitidissima Chi samples,and 5 polyphenolic components were identified by further analysis with UPLC-QTOF-MS,such as 3'-methy-4'-glucoside-ellagic acid,ellagic acid,okicamelliaside,3'-methy ellagic acid,3,4-o,o-methylidyne-ellagic acid. The fingerprint similarity of the 10 batch samples was all higher than 0.90.Samples from different batches were classified into 4 groups based on cluster analysis and principal component analysis. This study provides a reference for the overall quality evaluation of Camellia nitidissima Chi.
In order to establish the HPLC fingerprint of the leaves of Camellia sinensis Chi to provide a basis for evaluating its quality,this study was performed by HPLC using an Agilent ZORBAX C18 column( 4.6 mm × 250 mm,5μm) with gradient mobile phase of acetonitrile-0.1% phosphoric acid at a flow rate of 1 m L/min. The detection wavelength was set at 254 nm,and the column temperature was 30℃. Ten batches of Camellia nitidissima Chi samples were processed by similarity evaluation system for chromatographic fingerprint of traditional Chinese medicine( 2012.1 Version) and SPSS software,and some common peaks were summarized preliminarily based on the UPLCQTOF-MS spectra. 10 common peaks were collected from 10 batches of Camellia nitidissima Chi samples,and 5 polyphenolic components were identified by further analysis with UPLC-QTOF-MS,such as 3'-methy-4'-glucoside-ellagic acid,ellagic acid,okicamelliaside,3'-methy ellagic acid,3,4-o,o-methylidyne-ellagic acid. The fingerprint similarity of the 10 batch samples was all higher than 0.90.Samples from different batches were classified into 4 groups based on cluster analysis and principal component analysis. This study provides a reference for the overall quality evaluation of Camellia nitidissima Chi.
引文
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[2]贺栋业,王丽丽,曹振辉,等.金花茶抗肿瘤功效研究进展[J].亚太传统医药,2015,11(3):68-72.
[3]韦锦斌,农彩丽,苏志恒,等.金花茶体外抗肿瘤活性及物质基础的初步研究[J].中国实验方剂学杂志,2014,20(10):169-174.
[4]邹登峰,张伟,梁臣艳,等.金花茶叶中黄酮类成分的HPLC指纹图谱研究[J].华西药学杂志,2015,30(4):462-464.
[5]杨立芳,刘洪存,罗佳,等.金花茶茶花HPLC指纹图谱的研究[J].食品工业科技,2016,37(6):86-89.