摘要
为了解金针菇对染料的降解作用机制,研究测定了金针菇发酵液木质素降解酶系酶活,探究不同处理发酵液对3种化合染料的脱色影响。结果表明,液体培养条件下,化合染料的降解既可通过金针菇发酵液中的菌丝吸附,又能利用其分泌的木质素降解酶系转化。对化合染料起转化作用的并不是漆酶,而以菌体吸附为主,菌体对不同化合染料的吸附有一定选择性;已灭活的菌体与未灭活的菌体对染料的吸附效果也不同,表明温度可以改变菌体表面结构,使其更易吸附溶液中的染料。该研究对当前染料的处理具有重要应用价值。
To explore the degradation mechanism of dyes, the ligninolytic enzyme system was investigated indetail by analyzing the enzyme activity of laccase, manganese peroxidase(Mn P) and lignin peroxidase(Li P). Atthe same time, the decolorization effects on three kinds of chemical dyes by fermentation liquid in differenttreatments were studied. The results showed that under liquid culture condition, the dyes could both beadsorbed by mycelium and transformed by the ligninolytic enzyme system which was secreted by Flammulina velutipes fermentation. However, the key substances to chemical dyes' transformation was not laccase butmycelium adsorption, and the adsorption of different chemical dyes had certain selectivity. The adsorptioneffect of the dyes by the inactivated mycelia and the non killed mycelia was also different, which suggested thatthe temperature could change the surface structure of the cell and make it easier to adsorb the dyes in thesolution. This study had essential application value for the current treatment of dyes.
引文
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