金针菇生物降解与染料脱色作用研究
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摘要
为了解金针菇对染料的降解作用机制,研究测定了金针菇发酵液木质素降解酶系酶活,探究不同处理发酵液对3种化合染料的脱色影响。结果表明,液体培养条件下,化合染料的降解既可通过金针菇发酵液中的菌丝吸附,又能利用其分泌的木质素降解酶系转化。对化合染料起转化作用的并不是漆酶,而以菌体吸附为主,菌体对不同化合染料的吸附有一定选择性;已灭活的菌体与未灭活的菌体对染料的吸附效果也不同,表明温度可以改变菌体表面结构,使其更易吸附溶液中的染料。该研究对当前染料的处理具有重要应用价值。
To explore the degradation mechanism of dyes, the ligninolytic enzyme system was investigated indetail by analyzing the enzyme activity of laccase, manganese peroxidase(Mn P) and lignin peroxidase(Li P). Atthe same time, the decolorization effects on three kinds of chemical dyes by fermentation liquid in differenttreatments were studied. The results showed that under liquid culture condition, the dyes could both beadsorbed by mycelium and transformed by the ligninolytic enzyme system which was secreted by Flammulina velutipes fermentation. However, the key substances to chemical dyes' transformation was not laccase butmycelium adsorption, and the adsorption of different chemical dyes had certain selectivity. The adsorptioneffect of the dyes by the inactivated mycelia and the non killed mycelia was also different, which suggested thatthe temperature could change the surface structure of the cell and make it easier to adsorb the dyes in thesolution. This study had essential application value for the current treatment of dyes.
To explore the degradation mechanism of dyes, the ligninolytic enzyme system was investigated indetail by analyzing the enzyme activity of laccase, manganese peroxidase(Mn P) and lignin peroxidase(Li P). Atthe same time, the decolorization effects on three kinds of chemical dyes by fermentation liquid in differenttreatments were studied. The results showed that under liquid culture condition, the dyes could both beadsorbed by mycelium and transformed by the ligninolytic enzyme system which was secreted by Flammulina velutipes fermentation. However, the key substances to chemical dyes' transformation was not laccase butmycelium adsorption, and the adsorption of different chemical dyes had certain selectivity. The adsorptioneffect of the dyes by the inactivated mycelia and the non killed mycelia was also different, which suggested thatthe temperature could change the surface structure of the cell and make it easier to adsorb the dyes in thesolution. This study had essential application value for the current treatment of dyes.
引文
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[15]郭艳艳,阮玲云,冯宏昌,等.不同营养条件下斑玉蕈菌丝生长及产酶特性[J].菌物学报,2014,33(3):697-705.
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[18]池玉杰,伊洪伟.木材白腐菌分解木质素的酶系统-锰过氧化物酶、漆酶和木质素过氧化物酶催化分解木质素的机制[J].菌物学报,2007,26(1):153-160.
[19]Maurya N S,Mittal A K.Removal mechanism of cationic dye(Safranin O)from the aqueous phase by dead macro fungus biosorbent[J].Water Sci Technol,2013,68(5):1048-1054.
[20]姚璐晔,薄冰,马超,等.增稠剂对金针菇液体发酵菌丝球物理特性的影响[J].食用菌,2013,5:12-13,19.
[21]刘效梅,辛宝平,李玮,等.开放系统中4株丝状真菌的成球生长及其对染料的吸附脱色[J].环境科学,2005,4:143-146.
[22]吴海霞,康敬万,李志峰,等.亚甲基蓝与DNA相互作用的电化学研究[J].分析测试学报,2006,25(4):1-5.
[23]邵娜娜,张充,顾青莹,等.菌群EL对苯胺蓝的脱色研究[J].南京农业大学学报,2014,1:133-137.
[24]蔡伟建,李峰,李济吾.镰刀菌(Fusarium sp.)HJ01对中性艳蓝GL的脱色降解特性研究[J].环境科学学报,2007,2:213-219.
[25]许爱清,向言词,李会东.运用丝状真菌生物质生物吸附镉(Ⅱ)污染物的研究[J].环境科学与技术,2013(4):55-61,124.
[26]任南琪,周显娇,郭婉茜,杨珊珊.染料废水处理技术研究进展[J].化工学报,2013,1:84-94.
[2]Fu Y,Viraraghavan T.Fungal decolorization of dye wastewater:a review[J].Bioresource Technology,2001,79(3):251-262.
[3]李蒙英,孟祥勋.真菌对染料废水脱色降解的研究进展[J].环境污染治理技术与设备,2002,10:19-22.
[4]王海磊,郭伟云,于广丽,等.金针菇降解木质素的能力及相关酶活研究[J].河南师范大学学报:自然科学版,2004,4:96-99.
[5]郭芬芬,曲阅闻,樊健,等.木质素降解酶系染料降解机理[J].科技信息,2008,19:383,406.
[6]于存,徐红云,池玉杰.白灵菇木质素降解酶系的检测及其染料脱色能力的研究[J].安徽农业科学,2014(6):1784-1787,1882.
[7]张力,邵喜霞,韩大勇.白腐真菌木质素降解酶系研究进展[J].吉林畜牧兽医,2009(2):12.
[8]杨新美.食用菌栽培学[M].北京:农业出版社,1988:489.
[9]苗人云,周洁,谭伟,等.金针菇栽培基质替代原料初步筛选研究[J].菌物学报,2014,2:411-424.
[10]黄良水,金群力.我国金针菇产业发展现状与前景展望[J].浙江农业科学,2011(6):1252-1256.
[11]杨念.金针菇抗氧化物的提取及其抗氧化性能的研究[D].广州:华南理工大学,2011.
[12]张丽霞,赵丽娟.金针菇多糖的抗氧化活性研究[J].西南农业学报,2014,1:240-243.
[13]许晓燕,余梦瑶,魏巍,等.金针菇子实体多糖分离纯化及结构和免疫活性研究[J].菌物学报,2014,33(2):375-384.
[14]傅国平,袁生,虞光华.金针菇栽培基质组分的降解及相关酶活的变化[J].微生物学通报,1999,4:237-241.
[15]郭艳艳,阮玲云,冯宏昌,等.不同营养条件下斑玉蕈菌丝生长及产酶特性[J].菌物学报,2014,33(3):697-705.
[16]马利.白腐真菌及其漆酶对不同结构染料的降解研究[D].武汉:华中科技大学,2013.
[17]胡开辉,刘建忠,孙淑静,等.斑玉蕈育种中漆酶转化体系建立的初步研究[J].菌物学报,2010,4:528-535.
[18]池玉杰,伊洪伟.木材白腐菌分解木质素的酶系统-锰过氧化物酶、漆酶和木质素过氧化物酶催化分解木质素的机制[J].菌物学报,2007,26(1):153-160.
[19]Maurya N S,Mittal A K.Removal mechanism of cationic dye(Safranin O)from the aqueous phase by dead macro fungus biosorbent[J].Water Sci Technol,2013,68(5):1048-1054.
[20]姚璐晔,薄冰,马超,等.增稠剂对金针菇液体发酵菌丝球物理特性的影响[J].食用菌,2013,5:12-13,19.
[21]刘效梅,辛宝平,李玮,等.开放系统中4株丝状真菌的成球生长及其对染料的吸附脱色[J].环境科学,2005,4:143-146.
[22]吴海霞,康敬万,李志峰,等.亚甲基蓝与DNA相互作用的电化学研究[J].分析测试学报,2006,25(4):1-5.
[23]邵娜娜,张充,顾青莹,等.菌群EL对苯胺蓝的脱色研究[J].南京农业大学学报,2014,1:133-137.
[24]蔡伟建,李峰,李济吾.镰刀菌(Fusarium sp.)HJ01对中性艳蓝GL的脱色降解特性研究[J].环境科学学报,2007,2:213-219.
[25]许爱清,向言词,李会东.运用丝状真菌生物质生物吸附镉(Ⅱ)污染物的研究[J].环境科学与技术,2013(4):55-61,124.
[26]任南琪,周显娇,郭婉茜,杨珊珊.染料废水处理技术研究进展[J].化工学报,2013,1:84-94.