阳江高本底辐射土壤微生物群落代谢特征研究
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摘要
为了探索高本底辐射对土壤微生物群落的影响。研究采用Biolog-Eco生态板法对广东阳江高本底辐射区及周边正常本底辐射地区的土壤微生物群落功能代谢进行了调查。通过对不同本底辐射样品土壤微生物群落平均颜色变化率(AWCD)、碳源利用类型、微生物群落多样性的差异分析,主成分分析及土壤理化性质与微生物群落代谢特征相关性分析。分析表明,尽管各样品间AWCD变化差异显著,但土壤微生物群落代谢特征与辐射背景的高低间无明显变化规律。相关性分析表明,微生物代谢特征与本底辐射强度呈现中度或弱相关,但相关性均不显著,两者间无统计学意义,说明高本底辐射对土壤微生物群落分布影响极小或无影响。
To explore the effect of high background radiation on the soil microbial community,Biolog-Eco method was employed to investigate metabolic characteristics of soil microbial communities under different background radiation around Yangjiang.By determining variation of average-well-color-development(AWCD) ,utilizing types of carbon sources and diversity of microbial communities,analysis of principal component and correlation between soil properties and metabolic characteristics was carried out then.Results displayed that AWCD were significantly different,which could be classed into three types,but no clue were obtained that high level background radiation resulted in alteration of AWCD,utilizing types of carbon source and soil microbial diversities.Though metabolic characteristics of soil microbial communities exhibited general or weak relevance with the background radiation,there was no statistical significance.It indicated there were small or not effect of the high background radiation on soil microbial communities.
To explore the effect of high background radiation on the soil microbial community,Biolog-Eco method was employed to investigate metabolic characteristics of soil microbial communities under different background radiation around Yangjiang.By determining variation of average-well-color-development(AWCD) ,utilizing types of carbon sources and diversity of microbial communities,analysis of principal component and correlation between soil properties and metabolic characteristics was carried out then.Results displayed that AWCD were significantly different,which could be classed into three types,but no clue were obtained that high level background radiation resulted in alteration of AWCD,utilizing types of carbon source and soil microbial diversities.Though metabolic characteristics of soil microbial communities exhibited general or weak relevance with the background radiation,there was no statistical significance.It indicated there were small or not effect of the high background radiation on soil microbial communities.
引文
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[19]韩世忠,高人,李爱萍,等.中亚热带地区两种森林植被类型土壤微生物群落结构[J].应用生态学报,2015,26(7):2151-2158.Han Shizhong,Gao ren,Li Aiping,et al.Soil microbial community structure of two types of forests in the midsubtropics of China[J].Chinese Journal of Applied Ecology,2015,26(7):2151-2158.(in Chinese)
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[21]Tang Y S,Wang L,Jia J W,et al.Response of soil microbial community in jiuduansha wetland to different successional stages and its implications for soil microbial respiration and carbon turnover[J].Soil Biology&Biochemistry,2011,43(3):638-646.
[22]王传耀,蒋梦月.γ射线对大肠杆菌(Escherichia coli)的辐射效应[J].核农学报,1992,(4):248-251.Wang Chuanyao,Jiang Mengyue.The Lethal of gama irradiation on the Escherichia coli[J].Journal of Nuclear Agricultural Sciences,1992(4):248-251.(in Chinese)
[23]Dalal A,Awad T,Lynne M,et al.Deinococcus depolymerans sp.nov.,a gamma-and uv-radiation-resistant bacterium,isolated from a naturally radioactive site[J].International Journal of Systematic&Evolutionary Microbiology,2011,61(Pt6):1448-1453.
[2]朱卫国,孙全富.高本底地区剂量学研究现状及今后研究方向[J].中国职业医学,2012(1):75-76,78.Zhu Weiguo,Sun Quanfu.Dosimetry study on current situation and further research directions in high background radiation area[J].China Occupational Medicine,2012(1):75-76,78.(in Chinese)
[3]朱卫国.阳江高本底地区居民器官剂量学研究[D].北京:中国疾病预防控制中心,2012.Zhu Weiguo.Study on organ dosimetry of residents in high background radiation area of Yangjiang[D].Beijing:China Center for Disease Control and Prevention,2012.(in Chinese)
[4]Hendry J H,Simon S L,Wojcik A,et al.Human exposure to high natural background radiation:what can it teach us about radiation risks[J].Journal of Radiological Protection,2009,29(2A):A29-A42.
[5]Luxin W.Health survey in high background radiation areas in China:high background radiation research group,China[J].Science,1980,209(4459):877-880.
[6]魏履新.阳江高本底辐射地区流行病学研究概述(1986-2004年阶段)[J].辐射防护通讯,2006,26(3):1-5.Wei Luxin.A brief overview of the epidemiological study in high background radiation area of Yangjiang,China(the period from 1986-2004)[J].Radiation Protection Bulletin,2006,26(3):1-5.(in Chinese)
[7]Zou J,Tao Z,Sun Q,et al.Cancer and non-cancer epidemiological study in the high background radiation area of yangjiang,China[J].International Congress,2005,1276(1276):97-101.
[8]Ghiassinejad M,Mortazavi S M,Cameron J R,et al.Very high background radiation areas of ramsar,iran:preliminary biological studies[J].Health Physics,2002,82(1):87-93.
[9]Sajad B,Shabestani M A,Saeid A,et al.The effects of residence duration in high background radiation areas on immune surveillance[J].Journal of Natural Science Biology&Medicine,2013,4(1):218-222.
[10]Muller A P,Mousseau T A.The effects of natural variation in background radioactivity on humans,animals and otherorganisms[J].Biological Reviews,2013,88(1):226-254.
[11]顾美英,徐万里,茆军,等.新疆绿洲农田不同连作年限棉花根际土壤微生物群落多样性[J].生态学报,2012,32(10):3031-3040.Gu Meiying,Xu Wangli,Mao Jun,et al.Microbial community diversity of rhizosphere soil in continuous cotton cropping system in Xinjiang[J].Acta Ecologica Sinica,2012,32(10):3031-3040.(in Chinese)
[12]顾美英,徐万里,唐光木,等.生物炭对灰漠土和风沙土土壤微生物多样性及与氮素相关微生物功能的影响[J].新疆农业科学,2014,51(5):926-934.Gu Meiying,Xu Wangli,Tang Guangmu,et al.Effects of biochar on soil microbial diversity and function related with n transformation in grey desert soil and aeolian sandy soil in Xinjiang[J].Xinjiang Agricultural Sciences,2014,51(5):926-934.(in Chinese)
[13]田雅楠,王红旗.Biolog法在环境微生物功能多样性研究中的应用[J].环境科学与技术,2011,34(3):50-57.Tian Yanan,Wang Hongqi.Application of Biolog to study of environmental microbial function diver sity[J].Environmental Science&Technology,2011,34(3):50-57.(in Chinese)
[14]张广帅,林勇明,马瑞丰,等.汶川地震灾区不同气候区土壤微生物群落碳源代谢多样性[J].环境科学,2015,36(2):669-677.Zhang Guangshuai,Lin Yongming,Ma Ruifeng,et al.Carbon source metabolic diversity of soil microbial community under different climate types in the area affected by Wenchuan earthquake[J].Environment,2015,36(2):669-677.(in Chinese)
[15]徐万里,唐光木,葛春辉,等.长期施肥对新疆灰漠土土壤微生物群落结构与功能多样性的影响[J].生态学报,2015,35(2):468-477.Xu Wanli,Tang Guangmu,Ge Chunhui,et al.Effects of long-term fertilization on diversities of soil microbial community structure and function in grey desert soil of Xinjiang[J].Acta Ecologica Sinica,2015,35(2):468-477.(in Chinese)
[16]郑有飞,石春红,吴芳芳,等.土壤微生物活性影响因子的研究进展[J].土壤通报,2009(5):1209-1214.Zheng Youfei,Shi Chunhong,Wu Fangfang,et al.Research advances on the factors influencing the activity of Soil Microorganism[J].Chinese Journal of Soil Science,2009(5):1209-1214.(in Chinese)
[17]Liu J,Sui Y,Yu Z,et al.High throughput se quencing analysis of biogeographical distribution of bacterial communities in the black soils of northeast China[J].Soil Biology&Biochemistry,2014,70(2):113-122.
[18]宋蒙亚,李忠佩,吴萌,等.不同种植年限设施菜地土壤微生物量和群落结构的差异[J].中国农业科学,2015,48(18):3635-3644.Song Mengya,Li Zhongpei,Wu Meng,et al.Changes in soil microbial biomass and community structure with cultivation chronosequence of greenhouse vegetables[J].Scientia Agricultura Sinica,2015,48(18):3635-3644.(in Chinese)
[19]韩世忠,高人,李爱萍,等.中亚热带地区两种森林植被类型土壤微生物群落结构[J].应用生态学报,2015,26(7):2151-2158.Han Shizhong,Gao ren,Li Aiping,et al.Soil microbial community structure of two types of forests in the midsubtropics of China[J].Chinese Journal of Applied Ecology,2015,26(7):2151-2158.(in Chinese)
[20]Liu J,Sui Y,Yu Z,et al.High throughput sequencing analysis of biogeographical distribution of bacterial communities in the black soils of northeast China[J].Soil Biology&Biochem istry,2014,70(2):113-122.
[21]Tang Y S,Wang L,Jia J W,et al.Response of soil microbial community in jiuduansha wetland to different successional stages and its implications for soil microbial respiration and carbon turnover[J].Soil Biology&Biochemistry,2011,43(3):638-646.
[22]王传耀,蒋梦月.γ射线对大肠杆菌(Escherichia coli)的辐射效应[J].核农学报,1992,(4):248-251.Wang Chuanyao,Jiang Mengyue.The Lethal of gama irradiation on the Escherichia coli[J].Journal of Nuclear Agricultural Sciences,1992(4):248-251.(in Chinese)
[23]Dalal A,Awad T,Lynne M,et al.Deinococcus depolymerans sp.nov.,a gamma-and uv-radiation-resistant bacterium,isolated from a naturally radioactive site[J].International Journal of Systematic&Evolutionary Microbiology,2011,61(Pt6):1448-1453.