富含半胱氨酸的酸性分泌蛋白对青光眼滤过泡瘢痕化影响的研究进展
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摘要
滤过泡瘢痕化是导致青光眼滤过术失败的主要原因。目前,临床上多应用结膜下注射丝裂霉素及5-氟尿嘧啶等抗代谢药物以减少瘢痕化的发生,一定程度上提高了手术成功率,但其伴随的细胞毒性等不良反应不可忽视。富含半胱氨酸的酸性分泌蛋白(secreted protein acidic and rich in cysteine,SPARC)作为一种基质细胞蛋白在眼内广泛分布,在创伤修复和组织重塑的过程中发挥重要作用。结膜下瘢痕化的小鼠模型中可观察到SPARC表达明显升高。研究表明SPARC可通过多种途径参与并调控滤过泡瘢痕化的形成,有望成为抗瘢痕化治疗的特异性新靶点。
Filtering bleb scarring is the main cause of glaucoma filtration surgery failure. Subconjunctival injection of antimetabolites, such as mitomycin and 5-fluorouracil, is widely used clinically to reduce the incidence of scarring, which improves the success rate of the surgery. However, accompanied side effects such as cytotoxicity should not be ignored. Secreted protein acidic and rich in cysteine(SPARC) as a matricellular protein is widely distributed in the eyes, which plays an important role in the process of wound repairing and tissue remodeling. The expression of SPARC is significantly elevated in the mouse model of subconjunctival scarring. Researches suggest that SPARC participates in and regulate the formation of bleb scarring through multiple pathways, therefore it may become a specific new target in the anti-scarring therapy.
Filtering bleb scarring is the main cause of glaucoma filtration surgery failure. Subconjunctival injection of antimetabolites, such as mitomycin and 5-fluorouracil, is widely used clinically to reduce the incidence of scarring, which improves the success rate of the surgery. However, accompanied side effects such as cytotoxicity should not be ignored. Secreted protein acidic and rich in cysteine(SPARC) as a matricellular protein is widely distributed in the eyes, which plays an important role in the process of wound repairing and tissue remodeling. The expression of SPARC is significantly elevated in the mouse model of subconjunctival scarring. Researches suggest that SPARC participates in and regulate the formation of bleb scarring through multiple pathways, therefore it may become a specific new target in the anti-scarring therapy.
引文
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[24]Rosset EM,Trombetta-Esilva J,Hepfer G,et al.SPARC and the N-propeptide of collagenⅠinfluence fibroblast proliferation and collagen assembly in the periodontal ligament[J].PLo S One,2017,12(2):e0173209.
[25]Chlenski A,Liu S,Guerrero L J,et al.SPARC expression is associated with impaired tumor growth,inhibited angiogenesis and changes in the extracellular matrix[J].Int J Cancer,2006,118(2):310-316.
[26]Przekora A,Zarnowski T,Ginalska G.A simple and effective protocol for fast isolation of human Tenon's fibroblasts from a single trabeculectomy biopsy:a comparison of cell behaviour in different culture media[J].Cell Mol Biol Lett,2017,22:5.
[27]Seet LF,Su R,Toh LZ,et al.In vitro analyses of the anti-fibrotic effect of SPARC silencing in human Tenon's fibroblasts:comparisons with mitomycin C[J].J Cell Mol Med,2012,16(6):1245-1259.
[28]Nakamura-Shibasaki M,Ko JA,Takenaka J,et al.Matrix metalloproteinase and cytokine expression in Tenon fibroblasts during scar formation after glaucoma filtration or implant surgery in rats[J].Cell Biochem Funct,2013,31(6):482-488.
[29]Zhou X,Tan FK,Guo X,et al.Attenuation of collagen production with small interfering RNA of SPARC in cultured fibroblasts from the skin of patients with scleroderma[J].Arthritis Rheum,2006,54(8):2626-2631.
[30]Seet LF,Su R,Barathi VA,et al.SPARC deficiency results in improved surgical survival in a novel mouse model of glaucoma filtration surgery[J].PLo S One,2010,5(2):e9415.
[2]Sawchyn AK,Slabaugh MA.Innovations and adaptations in trabeculectomy[J].Curr Opin Ophthalmol,2015,27(2):158-163.
[3]Md Noh SM,Sheikh Abdul Kadir SH,Bannur ZM,et al.Effects of ranibizumab on the extracellular matrix production by human Tenon's fibroblast[J].Exp Eye Res,2014,127:236-242.
[4]De Fendi LI,Arruda GV,Scott IU,et al.Mitomycin C versus 5-fluorouracil as an adjunctive treatment for trabeculectomy:a meta-analysis of randomized clinical trials[J].Clin Exp Ophthalmol,2014,41(8):798-806.
[5]Yamamoto T,Sawada A,Mayama C,et al.The 5-year incidence of bleb-related infection and its risk factors after filtering surgeries with adjunctive mitomycin C:collaborative bleb-related infection incidence and treatment study 2[J].Ophthalmology,2014,121(5):1001-1006.
[6]Al-Dabbagh N,Al-Shahrani H,Al-Dohayan N,et al.The SPARC-related modular calcium binding protein 2(SMOC2)gene polymorphism in primary glaucoma:a case-control study[J].Clin Ophthalmol,2017,11:549-555.
[7]Oh DJ,Kang MH,Ooi YH,et al.Overexpression of SPARC in human trabecular meshwork increases intraocular pressure and alters extracellular matrix[J].Invest Ophthalmol Vis Sci,2013,54(5):3309-3319.
[8]Wong SL,Sukkar MB.The SPARC protein:an overview of its role in lung cancer and pulmonary fibrosis and its potential role in chronic airways disease[J].Br J Pharmacol,2017,174(1):3-14.
[9]Toba H,de Castro Brás LE,Baicu CF,et al.Increased ADAMTS1 mediates SPARC-dependent collagen deposition in the aging myocardium[J].Am JPhysiol Endocrinol Metab,2016,310(11):E1027-E1035.
[10]Seet LF,Tan YF,Toh LZ,et al.Targeted therapy for the post-operative conjunctiva:SPARC silencing reduces collagen deposition[J].Br J Ophthalmol,2018,102(10):1460-1470.
[11]Yamanaka O,Kitano-Izutani A,Tomoyose K,et al.Pathobiology of wound healing after glaucoma filtration surgery[J].BMC Ophthalmol,2015,15(Suppl 1):157.
[12]Nakamura M,Tokura Y.Epithelial-mesenchymal transition in the skin[J].JDermatol Sci,2011,61(1):7-13.
[13]Chatterjee A,Villarreal G Jr,Rhee DJ.Matricellular proteins in the trabecular meshwork:review and update[J].J Ocul Pharmacol Ther,2014,30(6):447-463.
[14]Seet LF,Tong L,Su R,et al.Involvement of SPARC and MMP-3 in the pathogenesis of human pterygium[J].Invest Ophthalmol Vis Sci,2012,53(2):587-595.
[15]Scavelli K,Chatterjee A,Rhee DJ.Secreted protein acidic and rich in cysteine in ocular tissue[J].J Ocul Pharmacol Ther,2015,31(7):396-405.
[16]Zhang Z,Miao Y,Wang J,et al.Matricellular protein levels in aqueous humor and surgical outcomes of trabeculectomy[J].Invest Ophthalmol Vis Sci,2018,59(10):3906-3910.
[17]Wei HY,Liu JL,Lv BJ,et al.SPARC modulates expression of extracellular matrix genes in human trabecular meshwork cells[J].Acta Ophthalmol,2012,90(2):E138-E143.
[18]Swaminathan SS,Oh DJ,Kang MH,et al.TGF-β2-mediated ocular hypertension is attenuated in SPARC-null mice[J].Invest Ophthalmol Vis Sci,2014,55(7):4084-4097.
[19]Kim M,Lee C,Payne R,et al.Angiogenesis in glaucoma filtration surgery and neovascular glaucoma:s review[J].Surv Ophthalmol,2015,60(6):524-535.
[20]Crawford TN,Alfaro DV 3rd,Kerrison JB,et al.Diabetic retinopathy and angiogenesis[J].Curr Diabetes Rev,2009,5(1):8-13.
[21]Arnold SA,Rivera LB,Miller AF,et al.Lack of host SPARC enhances vascular function and tumor spread in an orthotopic murine model of pancreatic carcinoma[J].Dis Model Mech,2010,3(1-2):57-72.
[22]Seet LF,Finger SN,Chu SW,et al.Novel insight into the inflammatory and cellular responses following experimental glaucoma surgery:a roadmap for inhibiting fibrosis[J].Curr Mol Med,2013,13(6):911-28.
[23]Sobeih D,Hussein KA,Said N,et al.Deletion of SPARC enhances retinal vaso-obliteration in mouse model of oxygen-induced retinopathy[J].HSOA JOphthalmol Clin Res,2014,1(1).pii:002.
[24]Rosset EM,Trombetta-Esilva J,Hepfer G,et al.SPARC and the N-propeptide of collagenⅠinfluence fibroblast proliferation and collagen assembly in the periodontal ligament[J].PLo S One,2017,12(2):e0173209.
[25]Chlenski A,Liu S,Guerrero L J,et al.SPARC expression is associated with impaired tumor growth,inhibited angiogenesis and changes in the extracellular matrix[J].Int J Cancer,2006,118(2):310-316.
[26]Przekora A,Zarnowski T,Ginalska G.A simple and effective protocol for fast isolation of human Tenon's fibroblasts from a single trabeculectomy biopsy:a comparison of cell behaviour in different culture media[J].Cell Mol Biol Lett,2017,22:5.
[27]Seet LF,Su R,Toh LZ,et al.In vitro analyses of the anti-fibrotic effect of SPARC silencing in human Tenon's fibroblasts:comparisons with mitomycin C[J].J Cell Mol Med,2012,16(6):1245-1259.
[28]Nakamura-Shibasaki M,Ko JA,Takenaka J,et al.Matrix metalloproteinase and cytokine expression in Tenon fibroblasts during scar formation after glaucoma filtration or implant surgery in rats[J].Cell Biochem Funct,2013,31(6):482-488.
[29]Zhou X,Tan FK,Guo X,et al.Attenuation of collagen production with small interfering RNA of SPARC in cultured fibroblasts from the skin of patients with scleroderma[J].Arthritis Rheum,2006,54(8):2626-2631.
[30]Seet LF,Su R,Barathi VA,et al.SPARC deficiency results in improved surgical survival in a novel mouse model of glaucoma filtration surgery[J].PLo S One,2010,5(2):e9415.