银杏叶提取物对大鼠臂丛根性撕脱再植后神经元轴突再生及再髓鞘化的影响
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摘要
目的探讨银杏叶提取物对大鼠臂丛根性撕脱再植后神经元轴突再生及再髓鞘化的影响。方法选取健康雌性SD大鼠72只,其中54只用手术建立C5-C7臂丛神经撕脱、C6神经根再植模型,将造模成功大鼠随机分为模型组、对照组和实验组,每组18只;假手术组18只接受假手术。假手术组及模型组均给予0. 9%Na Cl 2 m L,qd,腹腔注射;对照组给予8. 5 mg·m L~(-1)氯化锂溶液2m L,qd,腹腔注射;实验组给予10. 0 mg·m L~(-1)银杏叶提取物溶液2 m L,qd,腹腔注射。各组均连续干预12周。对大鼠运动及感觉神经功能、再生运动神经元数量、肌皮神经再生神经轴突的数量和再髓鞘化程度,以及肌皮神经Slit1蛋白、Slit2蛋白表达水平进行检测。结果治疗后,实验组、对照组、模型组和假手术组的Terzis梳洗试验评分分别为(4. 21±0. 59),(3. 73±0. 51),(0. 22±0. 03)和(4. 83±0. 67)分,痛阈压力差分别为(1. 45±0. 16),(2. 26±0. 31),(4. 53±0. 62)和(0. 37±0. 05) N,停留时间差分别为(4. 02±0. 37),(6. 47±0. 75),(10. 85±1. 28)和(1. 53±0. 21) s,再生运动神经元数量分别为(414. 25±43. 63),(365. 54±35. 48),(175. 47±21. 17)和(82. 17±10. 23)个,肌皮神经轴突总数分别为(16. 64±2. 18),(13. 43±1. 53),(8. 65±1. 12)和(1. 62±0. 19)×10~3·mm~(-2),有髓轴突数量分别为(9. 27±1. 28),(6. 84±0. 73),(4. 37±0. 56)和(0. 83±0. 11)×103·mm~(-2),再生轴突髓鞘厚度分别为(0. 77±0. 11),(0. 65±0. 08),(0. 46±0. 06)和(0. 89±0. 12)μm,肌皮神经Slit1蛋白IOD值分别为(130. 59±14. 28)×10~2,(116. 84±12. 14)×10~2,(99. 75±11. 14)×10~2和(80. 32±10. 12)×10~2,Slit2蛋白IOD值分别为(224. 86±30. 45)×10~2,(165. 86±22. 85)×10~2,(127. 54±15. 45)×10~2和(104. 83±13. 24)×10~2,实验组的上述指标与假手术组、模型组、对照组比较,差异均有统计学意义(均P <0. 05)。结论银杏叶提取物能促进大鼠臂丛根性撕脱再植后神经元轴突再生及再髓鞘化,加速运动和感觉神经功能恢复,其作用机制可能与促进神经组织表达Slit1蛋白和Slit2蛋白有关。
Objective To analysis the effect of ginkgo biloba extract on neuronal axonal regeneration and remyelination in rats after replantation brachial plexus root avulsion. Methods Seventy-two healthy female SD rats were selected,54 of them were successfully established C5-C7 brachial plexus avulsion and C6 nerve root replantation model C5-C7 brachial plexus avulsion and C6 nerve root replantation model by surgery,the model rats were randomly divided into model group,control groupand test group,each with 18 rats,and 18 rats in sham-operation group underwent sham surgery. The sham-operation group and model group were given physiological saline 2 mL by intraperitoneal-injection,qd; the control group was given 8. 5 mg ·mL~(-1) lithium chloride solution 2 mL by intraperitoneal-injection,qd; the test group was given1 0. 0 mg·mL~(-1) ginkgo biloba extract solution 2 mL by intraperitoneal-injection,qd. Each group received continuous intervention for 12 weeks. Motor and sensory neurological function,the number of regenerated motor neurons,the number of regeneration axons and the degree of re myelination in musculocutaneous nerve,the expression levels of Slit1 protein and Slit2 protein in musculocutaneous nerve were detected. Results After treatment,the main indexes of test,control,model and sham-operation groups were compared: the Terzis grooming test scores were( 4. 21 ± 0. 59),( 3. 73 ± 0. 51),( 0. 22 ± 0. 03) and( 4. 83 ± 0. 67) scores, the pain threshold pressure difference were( 1. 45 ± 0. 16),( 2. 26 ± 0. 31),( 4. 53 ± 0. 62) and( 0. 37 ± 0. 05) N,the residence time difference were( 4. 02 ± 0. 37),( 6. 47 ± 0. 75),( 10. 85 ± 1. 28) and( 1. 53 ± 0. 21) s,the number of regenerated motor neurons were( 414. 25 ± 43. 63),( 365. 54 ± 35. 48),( 175. 47 ± 21. 17) and( 82. 17 ± 10. 23) neurons,the total number of nerve axons were( 16. 64 ± 2. 18),( 13. 43 ± 1. 53),( 8. 65 ± 1. 12) and( 1. 62 ± 0. 19) × 10~3·mm~(-2),the number of myelinated axons were( 9. 27 ± 1. 28),( 6. 84 ± 0. 73),( 4. 37 ± 0. 56) and( 0. 83 ± 0. 11) × 10~3·mm~(-2),the myelin sheath thickness of regenerated axon were( 0. 77 ± 0. 11),( 0. 65 ± 0. 08),( 0. 46 ± 0. 06) and( 0. 89 ± 0. 12) μm,the IOD value of Slit1 protein were( 130. 59 ± 14. 28) × 10~2,( 116. 84 ± 12. 14) × 10~2,( 99. 75 ± 11. 14) × 10~2 and( 80. 32 ± 10. 12) × 10~2,the IOD value of Slit2 protein were( 224. 86 ± 30. 45) × 10~2,( 165. 86 ± 22. 85) × 10~2,( 127. 54 ± 15. 45) × 102 and( 104. 83 ± 13. 24) × 102,the differences between the test group and sham-operation,model and control groups were statistically significant( all P < 0. 05). Conclusion Ginkgo biloba extract can promote axonal regeneration and remyelination in rats after replantation brachial plexus root avulsion,accelerate the recovery of motor and sensory nerve function,the mechanism of action may be related to promoting the expression of Slit1 protein and Slit2 protein in nerve tissue.
Objective To analysis the effect of ginkgo biloba extract on neuronal axonal regeneration and remyelination in rats after replantation brachial plexus root avulsion. Methods Seventy-two healthy female SD rats were selected,54 of them were successfully established C5-C7 brachial plexus avulsion and C6 nerve root replantation model C5-C7 brachial plexus avulsion and C6 nerve root replantation model by surgery,the model rats were randomly divided into model group,control groupand test group,each with 18 rats,and 18 rats in sham-operation group underwent sham surgery. The sham-operation group and model group were given physiological saline 2 mL by intraperitoneal-injection,qd; the control group was given 8. 5 mg ·mL~(-1) lithium chloride solution 2 mL by intraperitoneal-injection,qd; the test group was given1 0. 0 mg·mL~(-1) ginkgo biloba extract solution 2 mL by intraperitoneal-injection,qd. Each group received continuous intervention for 12 weeks. Motor and sensory neurological function,the number of regenerated motor neurons,the number of regeneration axons and the degree of re myelination in musculocutaneous nerve,the expression levels of Slit1 protein and Slit2 protein in musculocutaneous nerve were detected. Results After treatment,the main indexes of test,control,model and sham-operation groups were compared: the Terzis grooming test scores were( 4. 21 ± 0. 59),( 3. 73 ± 0. 51),( 0. 22 ± 0. 03) and( 4. 83 ± 0. 67) scores, the pain threshold pressure difference were( 1. 45 ± 0. 16),( 2. 26 ± 0. 31),( 4. 53 ± 0. 62) and( 0. 37 ± 0. 05) N,the residence time difference were( 4. 02 ± 0. 37),( 6. 47 ± 0. 75),( 10. 85 ± 1. 28) and( 1. 53 ± 0. 21) s,the number of regenerated motor neurons were( 414. 25 ± 43. 63),( 365. 54 ± 35. 48),( 175. 47 ± 21. 17) and( 82. 17 ± 10. 23) neurons,the total number of nerve axons were( 16. 64 ± 2. 18),( 13. 43 ± 1. 53),( 8. 65 ± 1. 12) and( 1. 62 ± 0. 19) × 10~3·mm~(-2),the number of myelinated axons were( 9. 27 ± 1. 28),( 6. 84 ± 0. 73),( 4. 37 ± 0. 56) and( 0. 83 ± 0. 11) × 10~3·mm~(-2),the myelin sheath thickness of regenerated axon were( 0. 77 ± 0. 11),( 0. 65 ± 0. 08),( 0. 46 ± 0. 06) and( 0. 89 ± 0. 12) μm,the IOD value of Slit1 protein were( 130. 59 ± 14. 28) × 10~2,( 116. 84 ± 12. 14) × 10~2,( 99. 75 ± 11. 14) × 10~2 and( 80. 32 ± 10. 12) × 10~2,the IOD value of Slit2 protein were( 224. 86 ± 30. 45) × 10~2,( 165. 86 ± 22. 85) × 10~2,( 127. 54 ± 15. 45) × 102 and( 104. 83 ± 13. 24) × 102,the differences between the test group and sham-operation,model and control groups were statistically significant( all P < 0. 05). Conclusion Ginkgo biloba extract can promote axonal regeneration and remyelination in rats after replantation brachial plexus root avulsion,accelerate the recovery of motor and sensory nerve function,the mechanism of action may be related to promoting the expression of Slit1 protein and Slit2 protein in nerve tissue.
引文
[1]王培荣,王玉路,李红.成人臂丛神经损伤的术后护理和康复[J].中国伤残医学,2015,23(17):128-129.
[2]董延召,刘福云,郭永成.分娩性臂丛神经损伤的早期手术治疗[J].实用医学杂志,2016,32(12):2072-2073.
[3]高杏.综合康复疗法治疗臂丛神经损伤1例体会[J].中国保健营养,2015,25(12):362-363.
[4]何方军,谭朝峰.臂丛神经损伤后影响上肢功能恢复的相关因素分析[J].中国实用神经疾病杂志,2015,18(11):82-83.
[5]王树锋.全臂丛神经损伤的治疗进展[J].临床外科杂志,2015,23(5):325-326.
[6]罗伟,周云龙,孙振毅,等.中药提取物在周围神经损伤治疗中的应用[J].世界最新医学信息文摘,2015,15(3):29-30.
[7]黄灿阳,张文明,朱维钦.神经根回植术治疗臂丛根性撕脱伤的实验研究[J].福建医药杂志,2011,33(2):71-73.
[8]BERTELLI J A,MIRA J C.The grasping test:a simple behavioral method for objective quantitative assessment of peripheral nerve regeneration in the rat[J].J Neuro Sci Meth,1995,58(1-2):151-155.
[9]邵为,仲海燕,龚凯,等.银杏叶提取物对大鼠脊髓损伤后神经细胞凋亡的影响及其机制研究[J].中华创伤骨科杂志,2014,16(10):885-890.
[10]杜旭,王瑞辉,王孟林,等.电针对坐骨神经损伤大鼠神经生长导向因子Slit1的影响[J].中国中医基础医学杂志,2014,20(3):368-371.
[2]董延召,刘福云,郭永成.分娩性臂丛神经损伤的早期手术治疗[J].实用医学杂志,2016,32(12):2072-2073.
[3]高杏.综合康复疗法治疗臂丛神经损伤1例体会[J].中国保健营养,2015,25(12):362-363.
[4]何方军,谭朝峰.臂丛神经损伤后影响上肢功能恢复的相关因素分析[J].中国实用神经疾病杂志,2015,18(11):82-83.
[5]王树锋.全臂丛神经损伤的治疗进展[J].临床外科杂志,2015,23(5):325-326.
[6]罗伟,周云龙,孙振毅,等.中药提取物在周围神经损伤治疗中的应用[J].世界最新医学信息文摘,2015,15(3):29-30.
[7]黄灿阳,张文明,朱维钦.神经根回植术治疗臂丛根性撕脱伤的实验研究[J].福建医药杂志,2011,33(2):71-73.
[8]BERTELLI J A,MIRA J C.The grasping test:a simple behavioral method for objective quantitative assessment of peripheral nerve regeneration in the rat[J].J Neuro Sci Meth,1995,58(1-2):151-155.
[9]邵为,仲海燕,龚凯,等.银杏叶提取物对大鼠脊髓损伤后神经细胞凋亡的影响及其机制研究[J].中华创伤骨科杂志,2014,16(10):885-890.
[10]杜旭,王瑞辉,王孟林,等.电针对坐骨神经损伤大鼠神经生长导向因子Slit1的影响[J].中国中医基础医学杂志,2014,20(3):368-371.