陆地棉杂交后代产量和品质性状QTL定位
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摘要
对机采棉产量和品质性状的数量性状基因座(QTL)进行定位,为培育高产优质新品种提供理论依据。本研究基于陆地棉‘中571’与‘中棉所49’构建的包括3 187个Bin标记的高密度遗传图谱,利用F2∶3群体田间表型数据,采用复合区间作图法(CIM),对产量和品质性状的QTL进行检测。结果表明:共检测到产量性状10个QTL,品质性状10个QTL。其中,产量性状中控制铃重QTL 5个,衣分QTL 1个,产量相关指标子指QTL4个,分布在2、8、15、17和20号染色体上,QTL区段中LOD值介于4.61～6.33,解释表型变异比例为2.93%～13.67%;品质性状中控制纤维上半部平均长度QTL 3个,断裂比强度QTL 2个,马克隆值QTL 5个,分布在1、2、15、23和25号染色体上,QTL区段中LOD值介于3.76～8.35,解释表型变异比例为2.97%～11.78%。此外,控制铃重的主效qBW-chr08-1,子指主效QTL qSI-chr15-1,纤维上半部平均长度主效QTLqFLq-chr15-1位点可用于精细定位和克隆。
The study was conducted to locate QTLs of fiber yield and quality traits in the crossing population of cotton,in order to provide a theoretical basis for cultivating high-yielding and quality varieties.Based on the high-density genetic map with 3 187 Bin markers constructed by‘Z571'and ‘CCRI 49'in upland cotton,the QTLs for yield and quality traits were detected by using composite interval mapping(CIM)with field phenotype data of F2∶3 population.The results indicated that 10 QTLs for yield traits and 10 QTLs for quality traits were detected.Among the yield traits,5 QTLs for controlling boll weight(BW),1 QTLs for lint percentage(LP),and 4 QTLs for seed index(SI)were located on chromosomes 2,8,15,17,and 20.The LOD scores of QTLs ranged from 4.61 to 6.33,explaining the phenotypic variation ranging from 2.93% to13.67%.3 QTLs for mean length of the upper half fiber(FL),2 QTLs for breaking tenacity of the fiber(FT),and 5 QTLs for the micronaire(Mc)were located on chromosomes 1,2,15,23 and 25.The LOD scores of QTLs ranged from 3.76 to 8.35,explaining the phenotypic variation ranging from 2.97% to 11.78%.In addition,qBW-chr8-3 amajor QTL for BW,qSI-chr15-1 amajor QTL for SI,and qFLq-chr15-1 amajor QTL for FL could be used for fine mapping and cloning.
The study was conducted to locate QTLs of fiber yield and quality traits in the crossing population of cotton,in order to provide a theoretical basis for cultivating high-yielding and quality varieties.Based on the high-density genetic map with 3 187 Bin markers constructed by‘Z571'and ‘CCRI 49'in upland cotton,the QTLs for yield and quality traits were detected by using composite interval mapping(CIM)with field phenotype data of F2∶3 population.The results indicated that 10 QTLs for yield traits and 10 QTLs for quality traits were detected.Among the yield traits,5 QTLs for controlling boll weight(BW),1 QTLs for lint percentage(LP),and 4 QTLs for seed index(SI)were located on chromosomes 2,8,15,17,and 20.The LOD scores of QTLs ranged from 4.61 to 6.33,explaining the phenotypic variation ranging from 2.93% to13.67%.3 QTLs for mean length of the upper half fiber(FL),2 QTLs for breaking tenacity of the fiber(FT),and 5 QTLs for the micronaire(Mc)were located on chromosomes 1,2,15,23 and 25.The LOD scores of QTLs ranged from 3.76 to 8.35,explaining the phenotypic variation ranging from 2.97% to 11.78%.In addition,qBW-chr8-3 amajor QTL for BW,qSI-chr15-1 amajor QTL for SI,and qFLq-chr15-1 amajor QTL for FL could be used for fine mapping and cloning.
引文
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[12]朱协飞,王鹏,司占峰,等.基于陆地棉背景的海岛棉染色体片段导入系产量性状QTL定位[J].作物学报,2017,43(12):1784-1790.
[13]Song W W,Wang M,Su W,et al.Genetic and phenotypic effects of chromosome segments introgressed fromGossypium barbadense into Gossypium hirsutum[J].BMC Genomics,2016 Mar 8;17:197.doi:10.1186/s12864-016-2560-2.
[14]张茂启,陈全家,苏秀娟,等.5个纤维品质性状在棉花海陆杂交群体中的QTL定位研究[J].西北农业学报,2015,24(11):64-71.
[15]戎福喜,汤丽魁,唐媛媛,等.海陆渐渗系棉花主要纤维品质性状的QTL定位分析[J].分子植物育种,2015,13(7):1509-1516.
[16]郭丽雪,石玉真,李俊文,等.陆海渐渗系分离群体(F2)产量和纤维品质性状的QTL定位[J].棉花学报,2015,27(6):550-560.
[17]黎波涛,陆地棉染色体片段代换系产量及纤维品质性状的遗传分析与主效QTL定位[D].北京:中国农业科学院,2016.
[18]孔令磊,石玉真,李邵琦,等.棉花陆海渐渗系双交分离群体产量和纤维品质性状的QTL定位[J].棉花学报,2018,30(2):119-127.
[19]齐海坤,严根土,王宁,等.机采棉杂交后代主要株型性状与产量和品质的关系[J].棉花学报,2017,29(5):456-465.
[2]毛树春,李付广.当代全球棉花产业[M].北京:中国农业出版社,2016:1-2.
[3]殷剑美,武耀廷,朱协飞,等.陆地棉产量与品质性状的主基因与多基因遗传分析[J].棉花学报,2003,15(2):67-72.
[4]潘玉欣,何易航,张岚,等.植物UGD基因家族系统发育分析及在棉纤维中的表达分析[J].河北农业大学学报,2018,41(3):29-36.
[5]艾先涛,梁亚军,龚照龙,等.新疆棉花产量和纤维品质性状SSR关联分析[J].新疆农业科学,2016,53(11):1969-1979.
[6]Zhai H C,Gong W K,Tan Y N,et al.Identification of chromosome segment substitution lines of gossypium barbadense introgressed in G.hirsutumand quantitative trait locus mapping for fiber quality and yield traits[J].PLoS One,2016,9:e0159101.
[7]Zhang Z,Shang H H,Shi Y Z,et al.Construction of a high-density genetic map by specific locus amplified fragment sequencing(SLAF-seq)and its application to Quantitative Trait Loci(QTL)analysis for boll weight in upland cotton(Gossypium hirsutum L.)[J].BMC Plant Biol,2016,16:79.
[8]Jamshed M,Jia F,Gong J,et al.Identification of stable quantitative trait loci(QTLs)for fiber quality traits across multiple environments in Gossypium hirsutum recombinant inbred line population[J].BMCGenomics,2016,17:197.
[9]Qi H K,Wang N,Qiao W Q,et al.Construction of a high-density genetic map using genotyping by sequencing(GBS)for quantitative trait loci(QTL)analysis of three plant morphological traits in upland cotton(Gossypium hirsutum L.)[J].Euphytica2017,213(4):83.
[10]Wang S,Basten C,Zeng Z.Windows QTL cartographer2.5.department of statistics,North Carolina State University,Raleigh,NC2007,http://statgen.ncsu.edu/qtlcart/WQTLCart.htm.
[11]李成奇,郭旺珍,张天真.衣分不同陆地棉品种的产量及产量构成因素的遗传分析[J].作物学报,2009,35(11):1990-1999.
[12]朱协飞,王鹏,司占峰,等.基于陆地棉背景的海岛棉染色体片段导入系产量性状QTL定位[J].作物学报,2017,43(12):1784-1790.
[13]Song W W,Wang M,Su W,et al.Genetic and phenotypic effects of chromosome segments introgressed fromGossypium barbadense into Gossypium hirsutum[J].BMC Genomics,2016 Mar 8;17:197.doi:10.1186/s12864-016-2560-2.
[14]张茂启,陈全家,苏秀娟,等.5个纤维品质性状在棉花海陆杂交群体中的QTL定位研究[J].西北农业学报,2015,24(11):64-71.
[15]戎福喜,汤丽魁,唐媛媛,等.海陆渐渗系棉花主要纤维品质性状的QTL定位分析[J].分子植物育种,2015,13(7):1509-1516.
[16]郭丽雪,石玉真,李俊文,等.陆海渐渗系分离群体(F2)产量和纤维品质性状的QTL定位[J].棉花学报,2015,27(6):550-560.
[17]黎波涛,陆地棉染色体片段代换系产量及纤维品质性状的遗传分析与主效QTL定位[D].北京:中国农业科学院,2016.
[18]孔令磊,石玉真,李邵琦,等.棉花陆海渐渗系双交分离群体产量和纤维品质性状的QTL定位[J].棉花学报,2018,30(2):119-127.
[19]齐海坤,严根土,王宁,等.机采棉杂交后代主要株型性状与产量和品质的关系[J].棉花学报,2017,29(5):456-465.