胰石蛋白在新生儿细菌感染性疾病中的作用研究
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摘要
目的监测新生儿细菌感染性疾病患儿血清胰石蛋白(PSP/reg)的水平变化,探讨血清PSP/reg在新生儿细菌感染性疾病中的作用价值。方法选择2016年6月至2017年5月在本院新生儿病房收治的细菌感染患儿92例纳入研究对象,分为重症感染组(36例)和普通感染组(56例),选择同期在本院新生儿病房收治的非感染性疾病的患儿58例为对照组。采用酶联免疫吸附试验(ELISA)定量检测血清PSP/reg、降钙素原(PCT)和C反应蛋白(CRP)水平。结果治疗前重症感染组PSP/reg、PCT和CRP水平明显高于普通感染组(P <0. 01)和对照组(P <0. 01),而普通感染组高于对照组(P <0. 01)。治疗第3天重症感染组和普通感染组PSP/reg、PCT和CRP水平较治疗前明显降低(P <0. 05),其中重症感染组的PSP/reg水平降低明显,但仍高于普通感染组(P <0. 01),治愈后两组PSP/reg、PCT和CRP水平均较治疗前明显降低(P <0. 01),且两组差异无统计学意义(P>0. 05)。结论血清PSP/reg水平与新生儿细菌感染性疾病的严重程度具有相关性,血清PSP/reg水平变化有助于评价新生儿细菌感染的治疗效果。
Objective To observe the clinical significance of serum pancreatic stone protein/regenerating protein(PSP/reg) in neonatal bacterial infectious diseases. Methods 92 cases of newborns with bacterial infection in our hospital from June 2016 to May 2017 were enrolled in the study. They were divided into severe infection group(36 cases) and mild infection group(56 cases) according to the severity of the infection. At the same period 58 newborns without infectious diseases in our hospital were also chosen as control group. The serum levels of PSP/reg,procalcitonin(PCT) and C-reactive protein(CRP) were quantitatively measured by enzyme-linked immunosorbent assay(ELISA). Results The serum levels of PSP/reg,PCT and CRP in severe infection group were significantly higher than those in mild infection group(P < 0. 01) and control group(P < 0. 01),while the levels of PSP/reg,PCT and CRP in mild infection group were significantly higher than those in control group(P < 0. 01). The levels of PSP/reg 3 day after treatment in both severe and mild infection group were significantly lower than those before treatment(P <0. 01),while the level of PSP/reg in severe infection group was higher than that in another group. After treatment,the levels of PSP/reg,PCT and CRP in the two infection groups were significantly decreased(P < 0. 01),and there was no significant difference between the two groups after healing(P > 0. 05).Conclusions The level of PSP/reg is correlated with the severity of neonatal bacterial infectious disease.The change of serum PSP/reg level may be useful in the evaluation of therapy in neonatal bacterial infection.
Objective To observe the clinical significance of serum pancreatic stone protein/regenerating protein(PSP/reg) in neonatal bacterial infectious diseases. Methods 92 cases of newborns with bacterial infection in our hospital from June 2016 to May 2017 were enrolled in the study. They were divided into severe infection group(36 cases) and mild infection group(56 cases) according to the severity of the infection. At the same period 58 newborns without infectious diseases in our hospital were also chosen as control group. The serum levels of PSP/reg,procalcitonin(PCT) and C-reactive protein(CRP) were quantitatively measured by enzyme-linked immunosorbent assay(ELISA). Results The serum levels of PSP/reg,PCT and CRP in severe infection group were significantly higher than those in mild infection group(P < 0. 01) and control group(P < 0. 01),while the levels of PSP/reg,PCT and CRP in mild infection group were significantly higher than those in control group(P < 0. 01). The levels of PSP/reg 3 day after treatment in both severe and mild infection group were significantly lower than those before treatment(P <0. 01),while the level of PSP/reg in severe infection group was higher than that in another group. After treatment,the levels of PSP/reg,PCT and CRP in the two infection groups were significantly decreased(P < 0. 01),and there was no significant difference between the two groups after healing(P > 0. 05).Conclusions The level of PSP/reg is correlated with the severity of neonatal bacterial infectious disease.The change of serum PSP/reg level may be useful in the evaluation of therapy in neonatal bacterial infection.
引文
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[2] Dong Y,Speer CP. Late-onset neonatal sepsis:recent developments[J]. Arch Dis Child Fetal Neonatal Ed,2015,100(3):F257-263. DOI:10. 1136/archdischild-2014-306213.
[3] Busani S,Girardis M. PSP/reg:a new stone in sepsis biomarkers?[J]. Crit Care,2012,16(4):143. DOI:10. 1186/cc11433.
[4] Lajaunias F. PSP and NT-proCNP assessed for predisposition to infection[J]. Crit Care,2013,17(5):450. DOI:10. 1186/cc12883.
[5] Fahmey SS,Mostafa H,Elhafeez NA,et al. Diagnostic and prognostic value of proadrenomedullin in neonatal sepsis[J]. Korean J Pediatr,2018,61(5):156-159. DOI:10. 3345/kjp. 2018. 61. 5.156.
[6] Jin CX,Hayakawa T,Ko SB,et al. Pancreatic stone protein/regenerating protein family in pancreatic and gastrointestinal diseases[J]. Intern Med,2011,50(15):1507-1516.
[7] Hou WR,Xie SN,Wang HJ,et al. Intramuscular delivery of a naked DNA plasmid encoding proinsulin and pancreatic regenerating III protein ameliorates type 1 diabetes mellitus[J]. Pharmacol Res,2011,63(4):320-327. DOI:10. 1016/j. phrs. 2010. 12.009.
[8] Rass AA,Talat MA,Arafa MA,et al. The Role of Pancreatic Stone Protein in Diagnosis of Early Onset Neonatal Sepsis[J]. Biomed Res Int,2016,2016:1035856. DOI:10. 1155/2016/1035856.
[9] Keel M,Hrter L,Reding T,et al. Pancreatic stone protein is highly increased during posttraumatic sepsis and activates neutrophil granulocytes[J]. Crit Care Med,2009,37(5):1642-1648.DOI:10. 1097/CCM. 0b013e31819da7d6.
[10] Kordek A,TorbéA,Tousty J,et al. The Determination of Procalcitonin Concentration in Early-Onset Neonatal Infection[J]. Clin Pediatr(Phila), 2017,56(4):333-340. DOI:10. 1177/0009922816656622.
[11] Klein HJ,Csordas A,Falk V,et al. Pancreatic stone protein predicts postoperative infection in cardiac surgery patients irrespective of cardiopulmonary bypass or surgical technique[J]. PLo S One,2015,10(3):e0120276. DOI:10. 1371/journal. pone. 0120276.
[12] Scherr A,Graf R,Bain M,et al. Pancreatic stone protein predicts positive sputum bacteriology in exacerbations of COPD[J].Chest,2013,143(2):379-387. DOI:10. 1378/chest. 12-0730.